BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0087.18
(433 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP081393 209 5e-55
AV777294 135 1e-32
AW719790 104 2e-29
TC13974 similar to UP|Q9SXE3 (Q9SXE3) T3P18.10, partial (14%) 124 3e-29
TC11595 weakly similar to UP|Q9FKE7 (Q9FKE7) Dimethylaniline mon... 61 4e-10
TC20127 56 1e-08
AW720305 45 2e-05
BP081724 42 2e-04
TC10426 similar to PIR|S22384|S22384 dihydrolipoamide dehydrogen... 31 0.45
AV409788 28 2.3
TC17211 similar to UP|PRT2_SEPOF (P80002) Spermatid-specific pro... 28 2.9
TC8422 UP|AAQ63461 (AAQ63461) Calmodulin 4 (Fragment), complete 27 5.0
AV410093 27 5.0
TC8857 similar to UP|Q8LKG0 (Q8LKG0) Drm4, partial (84%) 27 6.6
TC14216 UP|Q84KK4 (Q84KK4) S-adenosyl-L-methionine: 2,7,4'-trihy... 27 6.6
BP037372 27 8.6
BI419523 27 8.6
>BP081393
Length = 433
Score = 209 bits (533), Expect = 5e-55
Identities = 98/98 (100%), Positives = 98/98 (100%)
Frame = -2
Query: 336 EMMKSIKELYHSREVANVPKRYTHSIADNDGFEYFDKYGDNAGFPKLEEWRKEICISSTV 395
EMMKSIKELYHSREVANVPKRYTHSIADNDGFEYFDKYGDNAGFPKLEEWRKEICISSTV
Sbjct: 432 EMMKSIKELYHSREVANVPKRYTHSIADNDGFEYFDKYGDNAGFPKLEEWRKEICISSTV 253
Query: 396 NMFANLETFRDSWNDDEMLQEVLKIPYFTQLGDHGFEN 433
NMFANLETFRDSWNDDEMLQEVLKIPYFTQLGDHGFEN
Sbjct: 252 NMFANLETFRDSWNDDEMLQEVLKIPYFTQLGDHGFEN 139
>AV777294
Length = 628
Score = 135 bits (341), Expect = 1e-32
Identities = 77/211 (36%), Positives = 114/211 (53%), Gaps = 31/211 (14%)
Frame = +3
Query: 230 LVKVAKEVHLSSNSAYISEGLSKVIHKYQNFHLHPQIDNLQEDGREWF------------ 277
+ VAKEVH+++ S + E + + N LH ID++ EDG+ F
Sbjct: 3 IATVAKEVHIAARS--VEEDKLGKVPGHDNLWLHSMIDSVHEDGKVVFQDGSAIAVDCIL 176
Query: 278 -------------------IEDNRVGPLYEHTFPPSLAPSLSFMGIPSKVLGPHFFESQA 318
++DNRVGPLY+H FPP+LAP LSF+G+P KV+ E Q+
Sbjct: 177 HCTGYKYDFPFLETEGLVTVDDNRVGPLYQHVFPPALAPWLSFVGLPWKVIPFPLCELQS 356
Query: 319 KWIAQLLSGKKILPSFEEMMKSIKELYHSREVANVPKRYTHSIADNDGFEYFDKYGDNAG 378
KWIA +LS + LPS EEM K I+ Y S E + PK YTH++A ++Y + D G
Sbjct: 357 KWIASILSNRIALPSQEEMAKDIEAFYVSLEASGTPKSYTHNLAFVQ-WDYNNWIADQCG 533
Query: 379 FPKLEEWRKEICISSTVNMFANLETFRDSWN 409
P +EEWRK++ +++ + E++RD W+
Sbjct: 534 VPAVEEWRKQMYKAASKSKLVRPESYRDEWD 626
>AW719790
Length = 449
Score = 104 bits (259), Expect(2) = 2e-29
Identities = 55/105 (52%), Positives = 71/105 (67%), Gaps = 4/105 (3%)
Frame = +1
Query: 36 VVFEQNHDIGGQWLYNPNVQCEDPLGKNPVLKV-HSSIYESLRLMSPREIMGFTDFPFLV 94
VVFEQ +GG W+Y P V+ DPLG++P ++ SS+YESLR PRE MGF D+PF+
Sbjct: 112 VVFEQGEQVGGTWVYTPEVE-SDPLGQDPKRRIIQSSMYESLRTNLPRESMGFRDYPFVR 288
Query: 95 KKG--RDMRRFPGHRELLFYLNDFCEWFGL-REVIRFNTRVEYVG 136
K G RD RR+PGHRE+L YL DF F + E++RF T V + G
Sbjct: 289 KDGKERDPRRYPGHREVLMYLKDFAXDFEIDGELVRFQTEVVFXG 423
Score = 42.0 bits (97), Expect(2) = 2e-29
Identities = 19/29 (65%), Positives = 24/29 (82%)
Frame = +3
Query: 10 KNVCVIGAGPSGLVATRELKREGHKVVVF 38
++V VIGAG +GLVA REL+REGH+V F
Sbjct: 33 RHVAVIGAGAAGLVAARELRREGHRVGCF 119
>TC13974 similar to UP|Q9SXE3 (Q9SXE3) T3P18.10, partial (14%)
Length = 528
Score = 124 bits (311), Expect = 3e-29
Identities = 61/135 (45%), Positives = 86/135 (63%)
Frame = +2
Query: 278 IEDNRVGPLYEHTFPPSLAPSLSFMGIPSKVLGPHFFESQAKWIAQLLSGKKILPSFEEM 337
IEDNRVGPLY+H FPP+LAPSLSF+G+ K H E Q+KW+A++LSGK +LP+ EEM
Sbjct: 2 IEDNRVGPLYKHVFPPALAPSLSFIGLTFKEAIFHVKELQSKWVARILSGKVLLPTEEEM 181
Query: 338 MKSIKELYHSREVANVPKRYTHSIADNDGFEYFDKYGDNAGFPKLEEWRKEICISSTVNM 397
M+ I+ Y E +PKRYTH++ +Y D G P LE+W +++ N+
Sbjct: 182 MEDIRNFYQFMEENGLPKRYTHALRPFQ-VDYKDWLVAQIGLPPLEDWMEQMYSECFKNL 358
Query: 398 FANLETFRDSWNDDE 412
E +RD W+D++
Sbjct: 359 VEMKENYRDEWDDNQ 403
>TC11595 weakly similar to UP|Q9FKE7 (Q9FKE7) Dimethylaniline monooxygenase
(N-oxide-forming)-like protein, partial (13%)
Length = 435
Score = 60.8 bits (146), Expect = 4e-10
Identities = 36/121 (29%), Positives = 59/121 (48%)
Frame = +2
Query: 12 VCVIGAGPSGLVATRELKREGHKVVVFEQNHDIGGQWLYNPNVQCEDPLGKNPVLKVHSS 71
+ +IGAG SG+ A ++L H +VFE IGG W +
Sbjct: 104 IAIIGAGVSGIAAAKQLSH--HNPIVFEATDSIGGIWRH--------------------C 217
Query: 72 IYESLRLMSPREIMGFTDFPFLVKKGRDMRRFPGHRELLFYLNDFCEWFGLREVIRFNTR 131
+Y +L S F+DFP+ ++ D +P + E+L YL ++ E F L ++++FNT+
Sbjct: 218 VYNCTKLQSQTWNYEFSDFPWPKRESTD---YPSYLEILEYLENYAERFDLNKLVKFNTK 388
Query: 132 V 132
V
Sbjct: 389 V 391
>TC20127
Length = 582
Score = 56.2 bits (134), Expect = 1e-08
Identities = 29/81 (35%), Positives = 48/81 (58%)
Frame = +1
Query: 147 NDDLKWVVRSKEKESDGVVEEVFDAVVVASGHYSHPKLPCIQGMDTWKRKQMHSHIYRTP 206
N W V++K + S V E +VVA+G + P++P ++G++ +K K +HS Y+
Sbjct: 340 NSHQNWRVKAKNRTSGHVEEYAGKFLVVATGETAEPRIPEVEGLEGFKGKVIHSTGYKNG 519
Query: 207 QPFGKEIVVVVGNSFSGQEIS 227
+ F + V+VVG+ SG EIS
Sbjct: 520 KEFKNQNVLVVGSGNSGMEIS 582
>AW720305
Length = 577
Score = 45.4 bits (106), Expect = 2e-05
Identities = 20/44 (45%), Positives = 28/44 (63%)
Frame = +3
Query: 7 QNSKNVCVIGAGPSGLVATRELKREGHKVVVFEQNHDIGGQWLY 50
+ K V V+G+GP+GL A +L + GH V VFE+ IGG +Y
Sbjct: 291 RTGKRVAVVGSGPAGLAAADQLNKMGHTVTVFERADRIGGLMMY 422
>BP081724
Length = 334
Score = 42.4 bits (98), Expect = 2e-04
Identities = 22/72 (30%), Positives = 35/72 (48%)
Frame = -1
Query: 341 IKELYHSREVANVPKRYTHSIADNDGFEYFDKYGDNAGFPKLEEWRKEICISSTVNMFAN 400
+ +LY E P+RYTHSI +Y G P LE+W +++ N+
Sbjct: 334 VTDLYQFMEENGFPQRYTHSIRPFQ-VDYKHWLVAQIGLPPLEDWMEQMLRECFKNVAEM 158
Query: 401 LETFRDSWNDDE 412
E +RD W+D++
Sbjct: 157 KENYRDEWDDNQ 122
>TC10426 similar to PIR|S22384|S22384 dihydrolipoamide dehydrogenase
precursor - garden pea {Pisum sativum;}
, partial (31%)
Length = 512
Score = 30.8 bits (68), Expect = 0.45
Identities = 18/52 (34%), Positives = 25/52 (47%)
Frame = +3
Query: 5 TNQNSKNVCVIGAGPSGLVATRELKREGHKVVVFEQNHDIGGQWLYNPNVQC 56
+ + +V VIG GP G VA + + G K E+ +GG L NV C
Sbjct: 138 SGSDQNDVVVIGGGPGGYVAAIKAAQLGLKTTCIEKRGTLGGTCL---NVGC 284
>AV409788
Length = 423
Score = 28.5 bits (62), Expect = 2.3
Identities = 12/28 (42%), Positives = 19/28 (67%)
Frame = +2
Query: 12 VCVIGAGPSGLVATRELKREGHKVVVFE 39
V +IGAG +G+ EL +GH+V ++E
Sbjct: 335 VAIIGAGLAGMSTAVELLDQGHEVDIYE 418
>TC17211 similar to UP|PRT2_SEPOF (P80002) Spermatid-specific protein T2
[Contains: Sperm protamine SP2], partial (31%)
Length = 540
Score = 28.1 bits (61), Expect = 2.9
Identities = 14/42 (33%), Positives = 24/42 (56%), Gaps = 6/42 (14%)
Frame = +2
Query: 12 VCVIGAGPSGLVATRELKREGH------KVVVFEQNHDIGGQ 47
VC++GAG +G L++ K++VFE+N +GG+
Sbjct: 68 VCIVGAGIAGSSVAHFLRKYSPEPTPTTKILVFERNAIVGGR 193
>TC8422 UP|AAQ63461 (AAQ63461) Calmodulin 4 (Fragment), complete
Length = 832
Score = 27.3 bits (59), Expect = 5.0
Identities = 10/29 (34%), Positives = 20/29 (68%)
Frame = +1
Query: 48 WLYNPNVQCEDPLGKNPVLKVHSSIYESL 76
+L+NP + E+P+ NP+L H+++ + L
Sbjct: 64 FLHNPFKERENPIQSNPILSHHTTMADQL 150
>AV410093
Length = 428
Score = 27.3 bits (59), Expect = 5.0
Identities = 16/49 (32%), Positives = 23/49 (46%), Gaps = 9/49 (18%)
Frame = +2
Query: 2 VSKTNQNSKN---------VCVIGAGPSGLVATRELKREGHKVVVFEQN 41
+S +N NS + VIG GP+G A L G + +FE+N
Sbjct: 134 ISASNSNSAHSSIAGRKLRAAVIGGGPAGASAAEALATGGIETFLFERN 280
>TC8857 similar to UP|Q8LKG0 (Q8LKG0) Drm4, partial (84%)
Length = 772
Score = 26.9 bits (58), Expect = 6.6
Identities = 10/29 (34%), Positives = 14/29 (47%)
Frame = -1
Query: 178 HYSHPKLPCIQGMDTWKRKQMHSHIYRTP 206
HYSH C +G W + + HI+ P
Sbjct: 550 HYSHILFICTKGKQKWIAPKQNKHIFGHP 464
>TC14216 UP|Q84KK4 (Q84KK4) S-adenosyl-L-methionine:
2,7,4'-trihydroxyisoflavanone 4'-O-methyltransferase,
complete
Length = 1696
Score = 26.9 bits (58), Expect = 6.6
Identities = 10/27 (37%), Positives = 16/27 (59%)
Frame = +1
Query: 262 LHPQIDNLQEDGREWFIEDNRVGPLYE 288
LHP ++ ++WF+EDN L+E
Sbjct: 703 LHPSSLDMWRSSKKWFLEDNEELTLFE 783
>BP037372
Length = 495
Score = 26.6 bits (57), Expect = 8.6
Identities = 16/46 (34%), Positives = 26/46 (55%), Gaps = 1/46 (2%)
Frame = +2
Query: 244 AYISEGLS-KVIHKYQNFHLHPQIDNLQEDGREWFIEDNRVGPLYE 288
A +SE +I+KYQN H + N ++G+E + +NRV L +
Sbjct: 359 ALVSENAEGSLINKYQNNHYGGETQNETKEGKE--VNNNRVTHLIQ 490
>BI419523
Length = 548
Score = 26.6 bits (57), Expect = 8.6
Identities = 16/60 (26%), Positives = 27/60 (44%)
Frame = -1
Query: 97 GRDMRRFPGHRELLFYLNDFCEWFGLREVIRFNTRVEYVGPLNYNGAFSGNDDLKWVVRS 156
GRD R+ GHR L + C + + E + V +N+ A +D+ K ++ S
Sbjct: 548 GRDHRKPIGHRHLGSH*KSLCPFTPIAEALILRDAVVTAVNMNWGKAIFESDNKKLILSS 369
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.319 0.138 0.423
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,292,585
Number of Sequences: 28460
Number of extensions: 123607
Number of successful extensions: 605
Number of sequences better than 10.0: 34
Number of HSP's better than 10.0 without gapping: 599
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 600
length of query: 433
length of database: 4,897,600
effective HSP length: 93
effective length of query: 340
effective length of database: 2,250,820
effective search space: 765278800
effective search space used: 765278800
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 57 (26.6 bits)
Lotus: description of TM0087.18