BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0086.17
(1019 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP066094 276 1e-79
BP084332 224 4e-59
AV780009 131 6e-31
BP052632 103 1e-22
BP075943 75 7e-14
TC16929 weakly similar to UP|Q9LFY6 (Q9LFY6) T7N9.5, partial (4%) 71 1e-12
BP043850 69 3e-12
BP057233 69 5e-12
BP063694 68 8e-12
TC10186 weakly similar to UP|Q9FH39 (Q9FH39) Copia-type polyprot... 47 2e-10
AV766665 62 5e-10
AV427921 59 5e-09
AU088980 57 2e-08
TC8952 similar to UP|O82607 (O82607) T2L5.9 protein, partial (3%) 56 3e-08
TC12832 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-rela... 55 4e-08
TC15664 weakly similar to UP|O81617 (O81617) F8M12.17 protein, p... 55 4e-08
TC17418 similar to UP|Q94AX2 (Q94AX2) AT5g39790/MKM21_80, partia... 50 1e-06
AV424544 50 2e-06
AV766088 50 2e-06
TC19389 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-rela... 49 4e-06
>BP066094
Length = 532
Score = 276 bits (705), Expect(3) = 1e-79
Identities = 134/149 (89%), Positives = 142/149 (94%)
Frame = +2
Query: 600 ARLEAVKLLISFSVNHNIILHQMDVKSAFLNGYISEEVYVHQPPGFKDEKNPDHVFKLKK 659
++ EA++LLISFSVNHNIILHQM+VKSAFLNGYISEEVYVHQPPG +DEKN DH+FKLKK
Sbjct: 86 SKTEAIRLLISFSVNHNIILHQMNVKSAFLNGYISEEVYVHQPPGXEDEKNSDHIFKLKK 265
Query: 660 SLYGLKQAPRAWYERLSSFLLENEFVRGKVDTTLFCKTYKDDILIVQIYVDDIIFGSANQ 719
SLYGLKQAPRAWYERLSSFLLENE VRGKVDTTLFCKTYKDDILIVQIYVDDIIFGSAN
Sbjct: 266 SLYGLKQAPRAWYERLSSFLLENEXVRGKVDTTLFCKTYKDDILIVQIYVDDIIFGSANP 445
Query: 720 FLCKEFSEMMQAEFEMSMMGELKYFMGIQ 748
LCKEFSEMMQAEFEM MMGELKYF+GIQ
Sbjct: 446 SLCKEFSEMMQAEFEMRMMGELKYFLGIQ 532
Score = 37.4 bits (85), Expect(3) = 1e-79
Identities = 17/18 (94%), Positives = 17/18 (94%)
Frame = +3
Query: 584 YSQQEGIDYTETFAPVAR 601
YSQQ GIDYTETFAPVAR
Sbjct: 39 YSQQ*GIDYTETFAPVAR 92
Score = 23.1 bits (48), Expect(3) = 1e-79
Identities = 10/10 (100%), Positives = 10/10 (100%)
Frame = +1
Query: 574 RNKARLVAQG 583
RNKARLVAQG
Sbjct: 10 RNKARLVAQG 39
>BP084332
Length = 368
Score = 224 bits (572), Expect = 4e-59
Identities = 110/112 (98%), Positives = 110/112 (98%)
Frame = +2
Query: 904 QSTIALSTAEAEYIPAAICSTQMLWMKHQLEDYQILESNIPIYCDNTAAISLSKNPILHS 963
QSTIALSTAEAEYI AAICSTQMLWMKHQLEDYQILESNIPIYCDNTAAISLSKNPILHS
Sbjct: 32 QSTIALSTAEAEYISAAICSTQMLWMKHQLEDYQILESNIPIYCDNTAAISLSKNPILHS 211
Query: 964 RAKHIEVKYHFIRDYVQKGVLLLKFVDIDHQWADIFTKPLAEDRFNFILKNL 1015
RAKHIEVKYHFIRDYVQKGVLLLKFVD DHQWADIFTKPLAEDRFNFILKNL
Sbjct: 212 RAKHIEVKYHFIRDYVQKGVLLLKFVDTDHQWADIFTKPLAEDRFNFILKNL 367
>AV780009
Length = 529
Score = 131 bits (329), Expect = 6e-31
Identities = 67/168 (39%), Positives = 104/168 (61%), Gaps = 6/168 (3%)
Frame = -1
Query: 841 AVKRILRYLKGSTNLGLMYKKTSEYKLSGYCDADYVGDRTERKSTSGNC*FLGSNLVSWA 900
A R+LRY+KG+ GL + S KL Y D+D+ G R+S +G FLG++L+SW
Sbjct: 526 AATRVLRYVKGAPAQGLFFSADSPLKLQAYSDSDWAGCPDTRRSVTGYSIFLGTSLISWR 347
Query: 901 SKRQSTIALSTAEAEY--IPAAICSTQMLWMKHQLEDYQILESN----IPIYCDNTAAIS 954
+K+Q+T++ S++EAEY + A +C Q W+ + +Q L+ N +P++CDN +A+
Sbjct: 346 TKKQTTVSRSSSEAEYRALAATVCEVQ--WLSYL---FQFLKLNVPLPVPLFCDNQSALH 182
Query: 955 LSKNPILHSRAKHIEVKYHFIRDYVQKGVLLLKFVDIDHQWADIFTKP 1002
++ NP H R KHIE+ H +R +Q G++ L + HQ ADIFTKP
Sbjct: 181 IAHNPTFHERTKHIELDCHVVRAKLQAGLIHLLPISTHHQLADIFTKP 38
>BP052632
Length = 489
Score = 103 bits (257), Expect = 1e-22
Identities = 49/114 (42%), Positives = 70/114 (60%)
Frame = -3
Query: 345 KTRYELWKNIKPNISYFHPFGCVCYVLNTKDRLHKFDAKSSKCLLLGYSDRSKGFRFYNT 404
KT YELW+ +P +SYFHPFGC ++LNT D + KFD K K +LLGYS SK + +N+
Sbjct: 487 KTPYELWRGRRPTVSYFHPFGCK*FILNTNDNIGKFDNKFDKRILLGYSSNSKAYIVFNS 308
Query: 405 DAKTIEESIHVRFDDKLDSDQSKLVEKFADLSINVSDKGKAPEEAEPEEEEPEE 458
+ +EESI+V+FDD +L + F +L ++ D+ E +P E EE
Sbjct: 307 RTQVVEESINVKFDD*SIEATYRLEKDFLNLDLSDQDEEVPTNEGQPSETSHEE 146
>BP075943
Length = 547
Score = 74.7 bits (182), Expect = 7e-14
Identities = 45/159 (28%), Positives = 78/159 (48%), Gaps = 2/159 (1%)
Frame = -3
Query: 707 IYVDDIIFGSANQFLCKEFSEMMQAEFEMSMMGELKYFMGIQVDQTPEGTYIHQSKYTKE 766
+YVDD++ + + + +F + +GE KYF+G+++ ++ G ++Q KY +
Sbjct: 515 LYVDDVLLAGNDMHEIQLVKSSLHDQFRIKDLGEAKYFLGLEIARSTSGIVLNQRKYALQ 336
Query: 767 LLKKFNMTELVIAKTPMHPTCILEKEDASGKVCQKL--YRGMIGSLLYLTASRPDILFSV 824
L+ + H T +G + YR ++G LLYL +RPDI F+V
Sbjct: 335 LISDSGHFGFQ-PRFYSHGTNSQTLGTNTGTPLTDIGSYRRIVGRLLYLNTTRPDITFAV 159
Query: 825 HLCARFQSDPRETHLTAVKRILRYLKGSTNLGLMYKKTS 863
+ ++F S P + H + L+YL GS GL Y +S
Sbjct: 158 NQLSQFLSAPTDIHEQQLTGFLKYL*GSPGSGLFYPASS 42
>TC16929 weakly similar to UP|Q9LFY6 (Q9LFY6) T7N9.5, partial (4%)
Length = 553
Score = 70.9 bits (172), Expect = 1e-12
Identities = 32/91 (35%), Positives = 53/91 (58%), Gaps = 1/91 (1%)
Frame = +1
Query: 928 WMKHQLEDYQI-LESNIPIYCDNTAAISLSKNPILHSRAKHIEVKYHFIRDYVQKGVLLL 986
W+ + L+D ++ E +YCDN +A ++ NP+ H R KHIE+ H +R+ +QKG++ L
Sbjct: 4 WLTYLLQDLKVPFEQPALVYCDNNSARHIAANPVFHERTKHIEIDCHIVRERIQKGLIHL 183
Query: 987 KFVDIDHQWADIFTKPLAEDRFNFILKNLNM 1017
+ ADI+TK L+ F+ I L +
Sbjct: 184 LPISSSEPLADIYTKALSPQNFHQICAKLGL 276
>BP043850
Length = 515
Score = 69.3 bits (168), Expect = 3e-12
Identities = 33/93 (35%), Positives = 54/93 (57%), Gaps = 1/93 (1%)
Frame = -1
Query: 924 TQMLWMKHQLEDYQILESN-IPIYCDNTAAISLSKNPILHSRAKHIEVKYHFIRDYVQKG 982
++++W++ L + L+S P++ DNT+AI ++ NP+ H +HIEV H +R+ +
Sbjct: 515 SEIIWLRGLLSELGFLQSQPTPLHADNTSAIQIAANPVYHEWTRHIEVDCHSVREAYDRR 336
Query: 983 VLLLKFVDIDHQWADIFTKPLAEDRFNFILKNL 1015
V+ L V Q ADI TK L R NF++ L
Sbjct: 335 VITLPHVSTSVQIADILTKSLTRQRHNFLVSKL 237
>BP057233
Length = 473
Score = 68.6 bits (166), Expect = 5e-12
Identities = 30/73 (41%), Positives = 49/73 (67%)
Frame = -2
Query: 945 IYCDNTAAISLSKNPILHSRAKHIEVKYHFIRDYVQKGVLLLKFVDIDHQWADIFTKPLA 1004
++CDN +A +L+ NP+LH+R+KHIE+ H+IRD V + +++ +V Q AD TKPL+
Sbjct: 445 LWCDNLSAKALASNPVLHARSKHIEIDVHYIRDQVLQNEVVVAYVPTTDQIADCLTKPLS 266
Query: 1005 EDRFNFILKNLNM 1017
RF+ + L +
Sbjct: 265 HTRFSQLRDKLGV 227
>BP063694
Length = 511
Score = 67.8 bits (164), Expect = 8e-12
Identities = 45/122 (36%), Positives = 61/122 (49%), Gaps = 3/122 (2%)
Frame = -2
Query: 133 VWHRRLGHVSMRKISQLSK---LNLVRGLPTLKFSSDALCEACQKDKFTKVHFKAKNVVS 189
+WH RLGHV+ I QL K L++ LP C +CQ K ++ F N
Sbjct: 360 LWHSRLGHVNFDIIKQLHKHGCLDVSSILP-----KPICCTSCQMAKSKRLVFHDNNK-R 199
Query: 190 TSRPLELLHIDLFGPVKTESIGGKRYGMVIVDDYSRWTWVKFLSRKDESHAVFSTFIAQV 249
S L+L+H DL GP SI G Y ++ VDD+SR+TW L RK + V F +
Sbjct: 198 ASAVLDLIHCDLRGPSPVASIDGFSYFVIFVDDFSRFTWFYPLKRKSDFSDVLLRFKVFM 19
Query: 250 QN 251
+N
Sbjct: 18 EN 13
>TC10186 weakly similar to UP|Q9FH39 (Q9FH39) Copia-type polyprotein, partial
(4%)
Length = 528
Score = 47.4 bits (111), Expect(2) = 2e-10
Identities = 25/51 (49%), Positives = 31/51 (60%)
Frame = +3
Query: 967 HIEVKYHFIRDYVQKGVLLLKFVDIDHQWADIFTKPLAEDRFNFILKNLNM 1017
+IE KYHF+RD V KG + LK D Q ADI TK L +RF + LN+
Sbjct: 69 NIETKYHFLRDQVTKGKISLKHCGTDLQVADIMTKGLKTERFRNMRAMLNV 221
Score = 35.4 bits (80), Expect(2) = 2e-10
Identities = 14/22 (63%), Positives = 18/22 (81%)
Frame = +2
Query: 948 DNTAAISLSKNPILHSRAKHIE 969
DN +AI L+KNP+ H R+KHIE
Sbjct: 5 DNKSAIDLAKNPVSHGRSKHIE 70
>AV766665
Length = 601
Score = 62.0 bits (149), Expect = 5e-10
Identities = 32/76 (42%), Positives = 46/76 (60%)
Frame = +3
Query: 840 TAVKRILRYLKGSTNLGLMYKKTSEYKLSGYCDADYVGDRTERKSTSGNC*FLGSNLVSW 899
T RI YLK ++ G +++K + + G+ ADY+G +R ST G FL NLV+W
Sbjct: 360 TFTTRIF*YLKANSRRGPLFQKEGKSSMDGFTYADYLGSIVDRLSTMGYYMFLSGNLVTW 539
Query: 900 ASKRQSTIALSTAEAE 915
SK+Q+ IA S+ EAE
Sbjct: 540 RSKQQNIIARSSGEAE 587
>AV427921
Length = 284
Score = 58.5 bits (140), Expect = 5e-09
Identities = 31/79 (39%), Positives = 42/79 (52%)
Frame = +1
Query: 291 TPQ*NGVVERKNRTLQEMARTMLQETGMAKYFWAEAVNTVCYIQNRISVRPILNKTRYEL 350
TPQ NGV ERKNRT+ M R++L +G+ K F EAV +I NR + N E
Sbjct: 22 TPQQNGVSERKNRTILNMVRSLLTMSGLPKSFLPEAVMWSLHILNRSPTLVVQNMMPEEA 201
Query: 351 WKNIKPNISYFHPFGCVCY 369
W +P + +F C+ Y
Sbjct: 202 WSGRQPAVDHFRISRCLAY 258
>AU088980
Length = 360
Score = 56.6 bits (135), Expect = 2e-08
Identities = 27/105 (25%), Positives = 48/105 (45%)
Frame = +2
Query: 295 NGVVERKNRTLQEMARTMLQETGMAKYFWAEAVNTVCYIQNRISVRPILNKTRYELWKNI 354
N +VERK+R + + R ++ + + K W AV Y+ N + + + Y+ N
Sbjct: 2 NSIVERKHRHILNVTRALMFHSYLPKNLWTFAVKHAAYLINXLPSPLLKGQCPYQFLNND 181
Query: 355 KPNISYFHPFGCVCYVLNTKDRLHKFDAKSSKCLLLGYSDRSKGF 399
P + FG +CY K D ++ KC+ LG+ + G+
Sbjct: 182 SPTLLDLKVFGTLCYSSTLTHNXQKLDPRARKCVFLGFKTGTXGY 316
>TC8952 similar to UP|O82607 (O82607) T2L5.9 protein, partial (3%)
Length = 550
Score = 56.2 bits (134), Expect = 3e-08
Identities = 29/82 (35%), Positives = 47/82 (56%), Gaps = 1/82 (1%)
Frame = +3
Query: 925 QMLWMKHQLEDYQILESN-IPIYCDNTAAISLSKNPILHSRAKHIEVKYHFIRDYVQKGV 983
+ LW+ + L D +I + IYCDN +A+ L+ N + H R ++IE+ H + V G+
Sbjct: 21 EALWLTYALADLRIASLLLVVIYCDNRSALHLAANSVFHKRTENIEIDCHIV*VKVLFGI 200
Query: 984 LLLKFVDIDHQWADIFTKPLAE 1005
L L V Q AD+FTK +++
Sbjct: 201 LHLLHVPSSDQVADVFTKTISQ 266
>TC12832 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-related Pol
polyprotein from transposon TNT 1-94 [Contains: Protease
; Reverse transcriptase ; Endonuclease] , partial (9%)
Length = 747
Score = 55.5 bits (132), Expect = 4e-08
Identities = 36/145 (24%), Positives = 70/145 (47%), Gaps = 9/145 (6%)
Frame = +2
Query: 677 SFLLENEFVRGKVDTTLFCKTYKD-DILIVQIYVDDIIFGSANQFLCKEFSEMMQAEFEM 735
SF++ + R D + K + D D +I+ +YVDD++ N+ +E + EF+M
Sbjct: 8 SFIMSLGYNRLSSDHCTYHKRFDDNDFIILLLYVDDMLVVGPNKDRVQELKAQLAREFDM 187
Query: 736 SMMGELKYFMGIQV--DQTPEGTYIHQSKYTKELLKKFNMTELVIAKTP------MHPTC 787
+G +G+Q+ D+ ++ Q Y +++L++FNM + TP + +
Sbjct: 188 KDLGPANKILGMQIHRDRKDRRIWLSQKNYLQKVLRRFNMQDCNPISTPLPVNYKLSSSM 367
Query: 788 ILEKEDASGKVCQKLYRGMIGSLLY 812
I E ++ + Y +GSL+Y
Sbjct: 368 IPSSEAERMEMSRVPYASAVGSLMY 442
>TC15664 weakly similar to UP|O81617 (O81617) F8M12.17 protein, partial (4%)
Length = 670
Score = 55.5 bits (132), Expect = 4e-08
Identities = 27/91 (29%), Positives = 50/91 (54%), Gaps = 1/91 (1%)
Frame = +1
Query: 928 WMKHQLEDYQI-LESNIPIYCDNTAAISLSKNPILHSRAKHIEVKYHFIRDYVQKGVLLL 986
W+ + L+D ++ S +YCD+ +A ++ N + H R KH+++ H +R+ +Q + L
Sbjct: 13 WLTYILQDLRVPFISPSLLYCDSQSARHIATNAVFHERTKHLDIDCHVVREKLQAKLFHL 192
Query: 987 KFVDIDHQWADIFTKPLAEDRFNFILKNLNM 1017
+ Q ADI TKPL F+ ++ L +
Sbjct: 193 LPISSVDQTADILTKPLESGPFSHLVSKLGV 285
>TC17418 similar to UP|Q94AX2 (Q94AX2) AT5g39790/MKM21_80, partial (20%)
Length = 739
Score = 50.4 bits (119), Expect = 1e-06
Identities = 35/99 (35%), Positives = 49/99 (49%), Gaps = 1/99 (1%)
Frame = -1
Query: 760 QSKYTKELLKKFNMTELVIAKTPMHPTCILEKEDASGK-VCQKLYRGMIGSLLYLTASRP 818
Q Y ++LKKF MT T + I GK V Y+ +IGS+ YL R
Sbjct: 739 QKIYVDDILKKFKMTNSKYISTTIGGKEIEAGRRNGGKRVDSTYYKSLIGSVRYLNTVRS 560
Query: 819 DILFSVHLCARFQSDPRETHLTAVKRILRYLKGSTNLGL 857
DI+ V L +RF +P + H +R LRY+KG+ G+
Sbjct: 559 DIVCGVGLRSRFM-EP*DCH*QGAQRSLRYIKGTLKDGI 446
>AV424544
Length = 276
Score = 50.1 bits (118), Expect = 2e-06
Identities = 27/92 (29%), Positives = 50/92 (54%), Gaps = 2/92 (2%)
Frame = +3
Query: 674 RLSSFLLENEFVRGKVDTTLFCKTYKDDILIVQIYVDDIIFGS--ANQFLCKEFSEMMQA 731
+LSS+L +++ D +LF K ++ +YVDD+I N+ C + +
Sbjct: 6 KLSSYLHILGYIQSAHDHSLFTKFRDASFTVILVYVDDLILAGNDLNEIQCVK--NKLDI 179
Query: 732 EFEMSMMGELKYFMGIQVDQTPEGTYIHQSKY 763
+F + +G LKYF+G++V ++ G ++ Q KY
Sbjct: 180 QFRIKDLGTLKYFLGLEVARSSCGLFLSQRKY 275
>AV766088
Length = 501
Score = 50.1 bits (118), Expect = 2e-06
Identities = 36/108 (33%), Positives = 56/108 (51%), Gaps = 1/108 (0%)
Frame = -3
Query: 131 Q*VWHRRLGHVSMRKISQLSKLNLVRGLPTLKFSSDALCEACQKDKFTKVHFKAKNVVST 190
Q +WH RLGH S + L + N L +S +CE+C K ++ F + N V T
Sbjct: 313 QSLWHSRLGHPSSSALRYL-RSNKFISYELLNYS--PVCESCVFGKHVRLPFVSSNNV-T 146
Query: 191 SRPLELLHIDLF-GPVKTESIGGKRYGMVIVDDYSRWTWVKFLSRKDE 237
P ++LH DL+ PV S G R+ ++ +DD++ + W LS K +
Sbjct: 145 VMPFDILHSDLWTSPVL--SSAGHRFYVLFLDDFTDFLWTFPLSNKSQ 8
>TC19389 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-related Pol
polyprotein from transposon TNT 1-94 [Contains: Protease
; Reverse transcriptase ; Endonuclease] , partial (6%)
Length = 498
Score = 48.9 bits (115), Expect = 4e-06
Identities = 34/114 (29%), Positives = 57/114 (49%), Gaps = 3/114 (2%)
Frame = +3
Query: 897 VSWASKRQSTIALSTAEAEYIPAAICSTQMLWMKHQLEDYQILESNIPIYC---DNTAAI 953
V+W S+ Q +ALSTAEAE+I A ++LWMK+ L++ + PI C A
Sbjct: 27 VAWPSRLQKCVALSTAEAEFIAATEACHELLWMKNFLQNAWF--HSHPILCCIVITKALF 200
Query: 954 SLSKNPILHSRAKHIEVKYHFIRDYVQKGVLLLKFVDIDHQWADIFTKPLAEDR 1007
+L++ + + +RD + +L L+ + D AD+ TK L ++
Sbjct: 201 TLARILLFIQDPSTLMFVIIGLRDVLNSKLLELEKIHTDDDGADMMTKSLPREK 362
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.321 0.136 0.402
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,749,777
Number of Sequences: 28460
Number of extensions: 225542
Number of successful extensions: 1289
Number of sequences better than 10.0: 78
Number of HSP's better than 10.0 without gapping: 1143
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1203
length of query: 1019
length of database: 4,897,600
effective HSP length: 99
effective length of query: 920
effective length of database: 2,080,060
effective search space: 1913655200
effective search space used: 1913655200
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 60 (27.7 bits)
Lotus: description of TM0086.17