BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0083.13
(195 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP030794 38 0.001
TC9450 similar to GB|AAM16172.1|20334832|AY094016 At2g39720/T5I7... 31 0.12
TC16643 similar to UP|Q9FIH3 (Q9FIH3) Serine/threonine protein k... 31 0.16
CN825698 30 0.36
AV428640 28 0.80
AV769781 28 1.0
AW719736 28 1.4
CB829437 27 1.8
BP033938 27 2.3
TC15352 similar to GB|AAM70520.1|21700793|AY124811 At2g22250/T26... 27 3.1
TC8088 similar to UP|Q9C5Q6 (Q9C5Q6) Fasciclin-like arabinogalac... 27 3.1
AV407897 27 3.1
TC13829 similar to UP|P93321 (P93321) Cdc2MsD protein, partial (... 27 3.1
TC14982 similar to UP|Q9FPI2 (Q9FPI2) At1g17620, partial (32%) 26 4.0
TC9107 similar to UP|Q9SWE2 (Q9SWE2) Alanine:glyoxylate aminotra... 26 5.2
TC12756 weakly similar to UP|Q96VI4 (Q96VI4) Protease 1, partial... 26 5.2
TC9356 weakly similar to UP|AGOL_ARATH (Q9SJK3) Argonaute-like p... 26 5.2
AV777727 25 6.8
TC20072 similar to UP|Q8V7C1 (Q8V7C1) ORF1 (Fragment), partial (... 25 6.8
AV423678 25 6.8
>BP030794
Length = 513
Score = 37.7 bits (86), Expect = 0.001
Identities = 30/100 (30%), Positives = 45/100 (45%)
Frame = +1
Query: 96 IRSLVSGLLTEDLDPQLKAGLLTFLELTEEMWELHRRRLEVNMLIWGLEVSATEHKGEET 155
I L LL EDL P+L+ L FL EE+ EL +LE+ +L V E++
Sbjct: 196 IHELTKALLEEDLRPELEKDLKEFLAFVEEIQEL--SQLELMLLEEKESVEGRIKTIEDS 369
Query: 156 GTMGEIEAELTSLLATREGLDRDLGAVHLRYNAMRVTPPF 195
E+ +L ++ E L+++ MR PPF
Sbjct: 370 LEKTELSIKLGNIEYALERLEKERADQRRECRRMRKDPPF 489
>TC9450 similar to GB|AAM16172.1|20334832|AY094016 At2g39720/T5I7.2
{Arabidopsis thaliana;}, partial (22%)
Length = 646
Score = 31.2 bits (69), Expect = 0.12
Identities = 22/74 (29%), Positives = 29/74 (38%), Gaps = 8/74 (10%)
Frame = -3
Query: 6 TKGHRPIRPCHVRK-EAVTAKRHTPPKARGRGLAKQRDPPGNDTYHP-------KSQASH 57
T H P P + + TA R+TPP R A R PP P +S
Sbjct: 626 TVAHAPPHPARILPCKRRTAPRYTPPSPRSPPSASTRSPPWKPADSPAGRIRSSRSARIA 447
Query: 58 PPQDKMTSPEAYLK 71
P D++ +P LK
Sbjct: 446 PTSDRIQTPAETLK 405
>TC16643 similar to UP|Q9FIH3 (Q9FIH3) Serine/threonine protein kinase-like
protein, partial (28%)
Length = 585
Score = 30.8 bits (68), Expect = 0.16
Identities = 29/100 (29%), Positives = 38/100 (38%)
Frame = +3
Query: 9 HRPIRPCHVRKEAVTAKRHTPPKARGRGLAKQRDPPGNDTYHPKSQASHPPQDKMTSPEA 68
H P RP H+R+ +RH PP+ R L P + Q +PPQ SP +
Sbjct: 21 HGPRRPSHLRRHRQLHRRHAPPRRRHPLL------PAPQIH----QIPNPPQQPDPSPPS 170
Query: 69 YLKALRAEALDCNLYYSYQTQVTAPGRIRSLVSGLLTEDL 108
Q Q+ R R LV G T+DL
Sbjct: 171 ---------------PKPQPQLLPLHRRRQLVLGSQTQDL 245
>CN825698
Length = 704
Score = 29.6 bits (65), Expect = 0.36
Identities = 15/37 (40%), Positives = 19/37 (50%), Gaps = 3/37 (8%)
Frame = +1
Query: 29 PPKARGRGLAKQRD---PPGNDTYHPKSQASHPPQDK 62
PP AR R RD PP Y+ +S S PP+D+
Sbjct: 199 PPPARRRDRRDDRDFDRPPNRRDYYDRSNNSPPPKDR 309
>AV428640
Length = 368
Score = 28.5 bits (62), Expect = 0.80
Identities = 17/53 (32%), Positives = 22/53 (41%), Gaps = 6/53 (11%)
Frame = +1
Query: 11 PIRPCHVRKEAVTAKRHTPP----KARGRGLAKQRDPPG--NDTYHPKSQASH 57
P+ P + T +RH PP G G +R PPG + H ASH
Sbjct: 178 PVNPPRLSHPCKTIRRHLPPPHGVHPHGGGFRPRRSPPGPPSPRQHLLQPASH 336
>AV769781
Length = 404
Score = 28.1 bits (61), Expect = 1.0
Identities = 20/70 (28%), Positives = 31/70 (43%), Gaps = 6/70 (8%)
Frame = +3
Query: 26 RHT---PPKARGRGLAKQRDPPGNDTYHPKSQASHPPQDKMTS---PEAYLKALRAEALD 79
RHT PKA R K++D PG + +Q+ + Q + T YL L+ ++ +
Sbjct: 165 RHTNAVKPKALSRSKPKKQDHPGENLSITNTQSINEKQKQKTKDLLSLLYLTLLQVDSAE 344
Query: 80 CNLYYSYQTQ 89
Y Q Q
Sbjct: 345 IQYQYGAQVQ 374
>AW719736
Length = 379
Score = 27.7 bits (60), Expect = 1.4
Identities = 16/53 (30%), Positives = 27/53 (50%)
Frame = +3
Query: 89 QVTAPGRIRSLVSGLLTEDLDPQLKAGLLTFLELTEEMWELHRRRLEVNMLIW 141
+V +P R L S LL EDL+ L+ + + + +E W L+++M W
Sbjct: 174 EVFSPFLARLLHSSLLIEDLEVSLRNFVDSKKKFVKEEWRNKLENLQMSMAEW 332
>CB829437
Length = 543
Score = 27.3 bits (59), Expect = 1.8
Identities = 23/112 (20%), Positives = 40/112 (35%)
Frame = +2
Query: 80 CNLYYSYQTQVTAPGRIRSLVSGLLTEDLDPQLKAGLLTFLELTEEMWELHRRRLEVNML 139
C+L +TAP + S L P++ L + + R+ +
Sbjct: 41 CSLAADQPPCLTAPPPLASTSPPHLLPSRSPKISKALTIRFHFPRSGFSRSQGRINLEQE 220
Query: 140 IWGLEVSATEHKGEETGTMGEIEAELTSLLATREGLDRDLGAVHLRYNAMRV 191
+W S H + +E E SL+A RE + + L+ A+ V
Sbjct: 221 MWKTMCSLLRHMTIPLRLLRHLELERMSLMAIRERMSLGHPCLTLKMGAVTV 376
>BP033938
Length = 479
Score = 26.9 bits (58), Expect = 2.3
Identities = 12/26 (46%), Positives = 16/26 (61%)
Frame = -3
Query: 90 VTAPGRIRSLVSGLLTEDLDPQLKAG 115
V PGR RS++S + P+LKAG
Sbjct: 360 VPTPGRSRSVLSAFFVKPKTPKLKAG 283
>TC15352 similar to GB|AAM70520.1|21700793|AY124811 At2g22250/T26C19.9
{Arabidopsis thaliana;}, partial (33%)
Length = 1061
Score = 26.6 bits (57), Expect = 3.1
Identities = 17/40 (42%), Positives = 19/40 (47%), Gaps = 1/40 (2%)
Frame = +1
Query: 7 KGH-RPIRPCHVRKEAVTAKRHTPPKARGRGLAKQRDPPG 45
KGH I P R EAV +RH P R R + RD G
Sbjct: 577 KGH*HFIEPTSQRCEAVQDRRHQRPSHRSRRSWRSRDSIG 696
>TC8088 similar to UP|Q9C5Q6 (Q9C5Q6) Fasciclin-like
arabinogalactan-protein 2, partial (69%)
Length = 1527
Score = 26.6 bits (57), Expect = 3.1
Identities = 13/28 (46%), Positives = 17/28 (60%)
Frame = +3
Query: 25 KRHTPPKARGRGLAKQRDPPGNDTYHPK 52
KR PP+ R R L +Q+ PP N HP+
Sbjct: 321 KRPLPPRPR-RLLWRQKAPPDNQRNHPR 401
>AV407897
Length = 295
Score = 26.6 bits (57), Expect = 3.1
Identities = 15/49 (30%), Positives = 22/49 (44%), Gaps = 6/49 (12%)
Frame = +1
Query: 18 RKEAVTAKRHTPPKARGRGLAKQRDP------PGNDTYHPKSQASHPPQ 60
R+EA+ HT P +R + + P D YH Q S+PP+
Sbjct: 43 RREAIETLLHTLPSSRSHDPSLRHSHTLRLTWPPRDHYHHPFQRSNPPK 189
>TC13829 similar to UP|P93321 (P93321) Cdc2MsD protein, partial (43%)
Length = 515
Score = 26.6 bits (57), Expect = 3.1
Identities = 15/39 (38%), Positives = 19/39 (48%)
Frame = +1
Query: 44 PGNDTYHPKSQASHPPQDKMTSPEAYLKALRAEALDCNL 82
P N+ +HP + A HPP P L L E LD +L
Sbjct: 310 PKNNNHHPSAAADHPP-----PPSKPLLYLVFEYLDTDL 411
>TC14982 similar to UP|Q9FPI2 (Q9FPI2) At1g17620, partial (32%)
Length = 1036
Score = 26.2 bits (56), Expect = 4.0
Identities = 15/50 (30%), Positives = 21/50 (42%)
Frame = +3
Query: 24 AKRHTPPKARGRGLAKQRDPPGNDTYHPKSQASHPPQDKMTSPEAYLKAL 73
A H+P + + PP P +Q S P + T+P A L AL
Sbjct: 6 ASLHSPTT*QTGSTLPPKPPPPQTAPPPPTQLSPPQRPNSTAPTALLTAL 155
>TC9107 similar to UP|Q9SWE2 (Q9SWE2) Alanine:glyoxylate aminotransferase 2
homolog, partial (81%)
Length = 1379
Score = 25.8 bits (55), Expect = 5.2
Identities = 12/29 (41%), Positives = 15/29 (51%)
Frame = -2
Query: 37 LAKQRDPPGNDTYHPKSQASHPPQDKMTS 65
L++Q+ YH K AS PPQD S
Sbjct: 421 LSQQKHQGNASQYHHKEIASSPPQDSEAS 335
>TC12756 weakly similar to UP|Q96VI4 (Q96VI4) Protease 1, partial (4%)
Length = 730
Score = 25.8 bits (55), Expect = 5.2
Identities = 20/79 (25%), Positives = 33/79 (41%), Gaps = 10/79 (12%)
Frame = +2
Query: 7 KGHRPIRPCHVRKEAVTAKRH----------TPPKARGRGLAKQRDPPGNDTYHPKSQAS 56
+GHR R H R+ + + H T G+G ++ R P + +S
Sbjct: 92 QGHRSGRHPHRRRSRRSLRHHNSTG*ASRGSTAITTHGKGASRLRLSPLS-----RSSLP 256
Query: 57 HPPQDKMTSPEAYLKALRA 75
PP + SPE + ++ RA
Sbjct: 257 EPPTKEPRSPEGHARSRRA 313
>TC9356 weakly similar to UP|AGOL_ARATH (Q9SJK3) Argonaute-like protein
At2g27880, partial (10%)
Length = 697
Score = 25.8 bits (55), Expect = 5.2
Identities = 13/38 (34%), Positives = 18/38 (47%)
Frame = +3
Query: 29 PPKARGRGLAKQRDPPGNDTYHPKSQASHPPQDKMTSP 66
P GRG + R PP + P S +SH P + +P
Sbjct: 99 PDAGTGRGRGRARAPP---SQQPPSSSSHSPAPVIGAP 203
>AV777727
Length = 467
Score = 25.4 bits (54), Expect = 6.8
Identities = 10/33 (30%), Positives = 16/33 (48%)
Frame = +3
Query: 124 EEMWELHRRRLEVNMLIWGLEVSATEHKGEETG 156
+ W++H R E LIWGL+ + + G
Sbjct: 135 KSFWKVHLVRFEFEGLIWGLKARTSLASSTKNG 233
>TC20072 similar to UP|Q8V7C1 (Q8V7C1) ORF1 (Fragment), partial (41%)
Length = 380
Score = 25.4 bits (54), Expect = 6.8
Identities = 15/33 (45%), Positives = 21/33 (63%)
Frame = +1
Query: 143 LEVSATEHKGEETGTMGEIEAELTSLLATREGL 175
LEV A+E T +++AEL SL+ATR+ L
Sbjct: 151 LEVVASE----ATARQADMQAELRSLMATRDEL 237
>AV423678
Length = 426
Score = 25.4 bits (54), Expect = 6.8
Identities = 18/56 (32%), Positives = 24/56 (42%), Gaps = 1/56 (1%)
Frame = +3
Query: 11 PIRPCHVRKEAVTAKR-HTPPKARGRGLAKQRDPPGNDTYHPKSQASHPPQDKMTS 65
PI P H +A +PP + R A PP + HP S AS P+ T+
Sbjct: 84 PISPFHPPPLTTSAAAISSPPPSPPRFTAP---PPSSSVSHPTSSASSAPRPAATA 242
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.316 0.133 0.395
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,530,065
Number of Sequences: 28460
Number of extensions: 49179
Number of successful extensions: 317
Number of sequences better than 10.0: 51
Number of HSP's better than 10.0 without gapping: 313
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 317
length of query: 195
length of database: 4,897,600
effective HSP length: 85
effective length of query: 110
effective length of database: 2,478,500
effective search space: 272635000
effective search space used: 272635000
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 53 (25.0 bits)
Lotus: description of TM0083.13