BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0082b.8
(726 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP060014 215 5e-59
TC13789 93 2e-19
TC13791 weakly similar to UP|RO52_HUMAN (P19474) 52 kDa Ro prote... 40 0.001
BP065703 40 0.002
TC19557 similar to PIR|E86326|E86326 protein F18O14.3 [imported]... 39 0.003
TC12104 39 0.004
AV411356 38 0.005
TC11793 similar to UP|AAR99376 (AAR99376) Ring domain containing... 37 0.009
TC8961 similar to GB|BAB71852.1|16902294|AB062739 kinesin-relate... 37 0.011
TC14669 similar to PIR|E86326|E86326 protein F18O14.3 [imported]... 37 0.011
TC9772 weakly similar to GB|AAG50239.1|12276054|AF304126 RING an... 37 0.011
BP084627 37 0.015
TC14940 36 0.019
TC11949 36 0.019
AV413409 35 0.032
BP036228 35 0.032
TC15777 similar to UP|AAR99376 (AAR99376) Ring domain containing... 35 0.042
TC10929 similar to UP|Q94AX6 (Q94AX6) AT3g23280/K14B15_17, parti... 33 0.12
TC10567 similar to UP|AAR99376 (AAR99376) Ring domain containing... 33 0.12
TC14881 33 0.21
>BP060014
Length = 366
Score = 215 bits (548), Expect(3) = 5e-59
Identities = 97/97 (100%), Positives = 97/97 (100%)
Frame = +3
Query: 340 GPMLHYANGKSVIGDAAMQPNVIHVHRCCVDWAPQVYFVDETCKNLKAEVARGAKLKCST 399
GPMLHYANGKSVIGDAAMQPNVIHVHRCCVDWAPQVYFVDETCKNLKAEVARGAKLKCST
Sbjct: 75 GPMLHYANGKSVIGDAAMQPNVIHVHRCCVDWAPQVYFVDETCKNLKAEVARGAKLKCST 254
Query: 400 CGLKGAALGCYVKSCKRTYHVPCAMDVSTCRWDQEKY 436
CGLKGAALGCYVKSCKRTYHVPCAMDVSTCRWDQEKY
Sbjct: 255 CGLKGAALGCYVKSCKRTYHVPCAMDVSTCRWDQEKY 365
Score = 26.2 bits (56), Expect(3) = 5e-59
Identities = 11/12 (91%), Positives = 11/12 (91%)
Frame = +1
Query: 330 CQSSETSEATGP 341
CQSSETSEAT P
Sbjct: 46 CQSSETSEATDP 81
Score = 25.0 bits (53), Expect(3) = 5e-59
Identities = 10/10 (100%), Positives = 10/10 (100%)
Frame = +2
Query: 320 LNPSTSICSF 329
LNPSTSICSF
Sbjct: 17 LNPSTSICSF 46
>TC13789
Length = 636
Score = 92.8 bits (229), Expect = 2e-19
Identities = 51/113 (45%), Positives = 70/113 (61%)
Frame = +3
Query: 610 LANEPKLFSGLKFYFSGDYVSSYKEDLEDLVEVGGGAVLTSKDKLEAKRHEFEVTSNLLI 669
L +PKLF G KFYF GD++ SYK L+D+V GG VL K L H+ LI
Sbjct: 9 LNKQPKLFDGYKFYFMGDFMPSYKGFLQDIVIAAGGLVLHRKPVLADHPHQ------TLI 170
Query: 670 VYNLDPPQGSKLGDEVSTLWQRLNEAEDLAANTGSQVIGHTWILESIAACKLQ 722
+Y+L+ P ++ + +R ++AE LA++TGS+V +TWIL SIAACKLQ
Sbjct: 171 IYSLELPNKCNPSEKDTIFSRRRSDAEVLASSTGSKVASNTWILNSIAACKLQ 329
>TC13791 weakly similar to UP|RO52_HUMAN (P19474) 52 kDa Ro protein
(Sjogren syndrome type A antigen) (SS-A) (Ro(SS-A)) (52
kDa ribonucleoprotein autoantigen Ro/SS-A), partial
(5%)
Length = 545
Score = 40.4 bits (93), Expect = 0.001
Identities = 20/52 (38%), Positives = 27/52 (51%), Gaps = 4/52 (7%)
Frame = +3
Query: 23 KLALELKCPLCLSLFEKPVLLPCDHLFCDSCLVD----SSLSGSECAVCKTK 70
K+ ++L CP+CL PV L C H+FC C S + G + AV K K
Sbjct: 39 KIDIDLTCPICLDTVFDPVSLTCGHIFCYMCACSAASVSIVDGLKSAVTKQK 194
>BP065703
Length = 480
Score = 39.7 bits (91), Expect = 0.002
Identities = 17/53 (32%), Positives = 30/53 (56%), Gaps = 1/53 (1%)
Frame = -3
Query: 28 LKCPLCLSLFEKPVLLPCDHLFCDSCLV-DSSLSGSECAVCKTKYAQTDIRNV 79
+KC +C ++ V++ C HLFC+ C+ + L +C C + Q+D+R V
Sbjct: 325 IKCTVCSDRPKEVVIVKCYHLFCNPCIQRNLELRHRKCPACGAAFGQSDVRFV 167
>TC19557 similar to PIR|E86326|E86326 protein F18O14.3 [imported] -
Arabidopsis thaliana {Arabidopsis thaliana;}, partial
(24%)
Length = 457
Score = 38.9 bits (89), Expect = 0.003
Identities = 17/45 (37%), Positives = 23/45 (50%), Gaps = 3/45 (6%)
Frame = +2
Query: 27 ELKCPLCLSLFEKPVLLPCDHLFCDSCL---VDSSLSGSECAVCK 68
+ +C +C L + PV+ C HLFC CL + EC VCK
Sbjct: 302 DFECNICFELAQDPVITLCGHLFCWPCLYRWLHHHSQSQECPVCK 436
>TC12104
Length = 771
Score = 38.5 bits (88), Expect = 0.004
Identities = 13/48 (27%), Positives = 23/48 (47%)
Frame = +1
Query: 30 CPLCLSLFEKPVLLPCDHLFCDSCLVDSSLSGSECAVCKTKYAQTDIR 77
C +C P+LL C H+FC+ C+ + C +C+ D++
Sbjct: 421 CAICQEKMHSPILLCCKHMFCEECVSEWFERERTCPLCRASVKPADLQ 564
>AV411356
Length = 358
Score = 38.1 bits (87), Expect = 0.005
Identities = 17/42 (40%), Positives = 23/42 (54%), Gaps = 3/42 (7%)
Frame = +2
Query: 30 CPLCLSLFEKPVLLPCDHLFCDSCL---VDSSLSGSECAVCK 68
C +CL L +PV+ C HLFC +C+ + EC VCK
Sbjct: 44 CNICLDLAREPVVTCCGHLFCWTCVYRWLHLHSDAKECPVCK 169
>TC11793 similar to UP|AAR99376 (AAR99376) Ring domain containing protein,
partial (23%)
Length = 527
Score = 37.4 bits (85), Expect = 0.009
Identities = 18/58 (31%), Positives = 32/58 (55%), Gaps = 11/58 (18%)
Frame = +3
Query: 30 CPLCLSLFEKPVLLPCDHLFCDSCL---VDSSLSGSE--------CAVCKTKYAQTDI 76
C +CL + PV+ C HL+C C+ ++S+ + SE C VCK++ +Q+ +
Sbjct: 351 CNICLDCVQDPVVTLCGHLYCWPCIYKWLNSNSASSENVEQNKPQCPVCKSEVSQSSL 524
>TC8961 similar to GB|BAB71852.1|16902294|AB062739 kinesin-related protein
{Arabidopsis thaliana;} , partial (8%)
Length = 735
Score = 37.0 bits (84), Expect = 0.011
Identities = 24/87 (27%), Positives = 35/87 (39%), Gaps = 17/87 (19%)
Frame = +1
Query: 3 DSGKPNNKSKLMNPWMLHFQKLALELK----------------CPLCLSLFEKPVLLPCD 46
D +P N+ P ++ + E+K C +C +LLPC
Sbjct: 124 DGSEPGNELPKEEPLVVRLKARMQEMKEKELKHLGNGDANSHVCKVCFESSTAAILLPCR 303
Query: 47 HL-FCDSCLVDSSLSGSECAVCKTKYA 72
H C SC SL+ SEC +C+T A
Sbjct: 304 HFCLCKSC----SLACSECPICRTSIA 372
>TC14669 similar to PIR|E86326|E86326 protein F18O14.3 [imported] -
Arabidopsis thaliana {Arabidopsis thaliana;}, partial
(77%)
Length = 1120
Score = 37.0 bits (84), Expect = 0.011
Identities = 16/43 (37%), Positives = 22/43 (50%), Gaps = 3/43 (6%)
Frame = +2
Query: 29 KCPLCLSLFEKPVLLPCDHLFCDSCL---VDSSLSGSECAVCK 68
+C +C L + P++ C HLFC CL + EC VCK
Sbjct: 125 ECNICFDLAQDPIITLCGHLFCWPCLYKWLHFHSQSRECPVCK 253
>TC9772 weakly similar to GB|AAG50239.1|12276054|AF304126 RING and zinc
finger protein {Caenorhabditis elegans;} , partial
(13%)
Length = 558
Score = 37.0 bits (84), Expect = 0.011
Identities = 14/43 (32%), Positives = 20/43 (45%)
Frame = +3
Query: 25 ALELKCPLCLSLFEKPVLLPCDHLFCDSCLVDSSLSGSECAVC 67
+L C +C F PV+ C H FC+ C + +C VC
Sbjct: 21 SLPFACFICRQPFVDPVVTKCKHYFCEHCALKHHAKNKKCFVC 149
>BP084627
Length = 327
Score = 36.6 bits (83), Expect = 0.015
Identities = 16/53 (30%), Positives = 30/53 (56%), Gaps = 3/53 (5%)
Frame = -2
Query: 30 CPLCLSLFEKPVLLPCDHLFCDSCLVDSSLSGS---ECAVCKTKYAQTDIRNV 79
C +C ++ V+ C HLFC++C++ ++GS +C C T + D++ V
Sbjct: 326 CSICQDRTKEVVITKCYHLFCNTCML--KVTGSRHRKCPQCGTSFGANDVKPV 174
>TC14940
Length = 781
Score = 36.2 bits (82), Expect = 0.019
Identities = 12/50 (24%), Positives = 26/50 (52%)
Frame = +2
Query: 30 CPLCLSLFEKPVLLPCDHLFCDSCLVDSSLSGSECAVCKTKYAQTDIRNV 79
CP+C+ + + C H+FC +C+ + + ++C C+ K ++ V
Sbjct: 149 CPICMGPLVEEMSTRCGHIFCKACIKAAISAQNKCPTCRKKVTVKELIRV 298
>TC11949
Length = 780
Score = 36.2 bits (82), Expect = 0.019
Identities = 13/31 (41%), Positives = 19/31 (60%)
Frame = +3
Query: 23 KLALELKCPLCLSLFEKPVLLPCDHLFCDSC 53
K+ ++L C +C+ PV L C H+FC SC
Sbjct: 108 KIDIDLTCSICMDTVFDPVSLTCGHIFCYSC 200
>AV413409
Length = 374
Score = 35.4 bits (80), Expect = 0.032
Identities = 21/60 (35%), Positives = 29/60 (48%), Gaps = 7/60 (11%)
Frame = +1
Query: 28 LKCPLCLSLFEKPVLL----PCDHLFCDSCLVDSSLSGSECAVCK---TKYAQTDIRNVP 80
L+C +CL+ FE L CDH+F C+ + S S C VC+ T +R VP
Sbjct: 43 LECAVCLNEFEDTETLRLIPKCDHVFHPECIDEWLSSHSTCPVCRANLTPQPGDSVRGVP 222
>BP036228
Length = 626
Score = 35.4 bits (80), Expect = 0.032
Identities = 19/62 (30%), Positives = 29/62 (46%), Gaps = 1/62 (1%)
Frame = +2
Query: 28 LKCPLCLSLFEKPVLLPCDHLFCDSCLVDSSLSGSE-CAVCKTKYAQTDIRNVPFVENMV 86
+ C C+ L ++PV PC H FC C G CA C+ + I + P + + +
Sbjct: 416 INCSFCMQLPDRPVTTPCGHNFCLKCFEKWIGQGKRTCANCR-RDIPAKIASQPRINSQL 592
Query: 87 AI 88
AI
Sbjct: 593 AI 598
>TC15777 similar to UP|AAR99376 (AAR99376) Ring domain containing protein,
partial (34%)
Length = 566
Score = 35.0 bits (79), Expect = 0.042
Identities = 17/58 (29%), Positives = 28/58 (47%), Gaps = 11/58 (18%)
Frame = +1
Query: 30 CPLCLSLFEKPVLLPCDHLFCDSCL--------VDSSLSGSE---CAVCKTKYAQTDI 76
C +CL + PV+ C HL+C C+ + S E C VCK++ +Q+ +
Sbjct: 289 CNICLDCVQDPVVTLCGHLYCWPCIYKWLNFYSISSDYEEKEEPICPVCKSEISQSSL 462
>TC10929 similar to UP|Q94AX6 (Q94AX6) AT3g23280/K14B15_17, partial (16%)
Length = 783
Score = 33.5 bits (75), Expect = 0.12
Identities = 18/69 (26%), Positives = 30/69 (43%), Gaps = 1/69 (1%)
Frame = +2
Query: 30 CPLCLSLFEKPVLLPCDHLF-CDSCLVDSSLSGSECAVCKTKYAQTDIRNVPFVENMVAI 88
C +CL + +PC H+ C SCL + C VC+ K ++ ++ +
Sbjct: 269 CVICLDAPAEGACIPCGHVAGCMSCLNEVKTKKWGCPVCRAK-----------IDQVIKL 415
Query: 89 YRSLDASFC 97
YR D+ C
Sbjct: 416 YRV*DSKHC 442
>TC10567 similar to UP|AAR99376 (AAR99376) Ring domain containing protein,
partial (27%)
Length = 423
Score = 33.5 bits (75), Expect = 0.12
Identities = 16/58 (27%), Positives = 29/58 (49%), Gaps = 11/58 (18%)
Frame = +2
Query: 30 CPLCLSLFEKPVLLPCDHLFCDSCLVD----SSLSGS-------ECAVCKTKYAQTDI 76
C +CL + PV+ C HL+C C+ S+S +C VCK++ +++ +
Sbjct: 86 CSICLDRVQDPVVTLCGHLYCWPCIYKWLNFHSVSSDHEEKKEPQCPVCKSEISESSL 259
>TC14881
Length = 609
Score = 32.7 bits (73), Expect = 0.21
Identities = 11/41 (26%), Positives = 23/41 (55%), Gaps = 1/41 (2%)
Frame = +3
Query: 29 KCPLCLSLFEKPVLLPCDHL-FCDSCLVDSSLSGSECAVCK 68
+C +C++ + +LPC H+ C C + L ++C +C+
Sbjct: 96 ECVICMTEPKDTAVLPCRHMCMCSDCAKELRLQSNKCPICR 218
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.316 0.132 0.396
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,802,149
Number of Sequences: 28460
Number of extensions: 205802
Number of successful extensions: 1144
Number of sequences better than 10.0: 76
Number of HSP's better than 10.0 without gapping: 1133
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1142
length of query: 726
length of database: 4,897,600
effective HSP length: 97
effective length of query: 629
effective length of database: 2,136,980
effective search space: 1344160420
effective search space used: 1344160420
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 59 (27.3 bits)
Lotus: description of TM0082b.8