BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0075.5
(667 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP054819 110 6e-25
BP041998 89 1e-24
TC10133 similar to UP|Q9LSS5 (Q9LSS5) Myosin heavy chain-like, p... 39 0.003
TC11361 38 0.005
TC13132 similar to UP|Q9FJA1 (Q9FJA1) Similarity to retroelement... 36 0.023
BP030371 33 0.19
BP037078 32 0.33
TC11021 32 0.33
TC9039 31 0.56
TC12218 30 0.96
TC8301 30 1.6
AV772411 28 3.6
CB827128 28 3.6
AI967308 28 4.8
BP065323 28 6.2
BP069559 28 6.2
BP028499 27 8.1
TC9247 similar to UP|Q8L6A5 (Q8L6A5) Replication factor C large ... 27 8.1
TC8135 similar to UP|GUN4_ARATH (Q9LX31) Tetrapyrrole-binding pr... 27 8.1
TC8647 similar to PIR|S32145|S32145 anther-specific protein - ra... 27 8.1
>BP054819
Length = 537
Score = 110 bits (276), Expect = 6e-25
Identities = 53/82 (64%), Positives = 61/82 (73%), Gaps = 6/82 (7%)
Frame = -3
Query: 10 PPILDGTNYDYWKARMMVFLKSMDSIAWKAIVKGWKHPV------IASTTELKPEDKWTK 63
PPILDGTNYDY K RM FLKS+D+ WKAIVKGWKHP ++T KPE++WT
Sbjct: 280 PPILDGTNYDY*KVRMTAFLKSIDNKTWKAIVKGWKHPTKVEVEGTSTTVVEKPEEEWTS 101
Query: 64 KEDDEALGNSKALNVIFNGVDK 85
ED+ ALGNSKALN IFNGVD+
Sbjct: 100 VEDEAALGNSKALNAIFNGVDR 35
>BP041998
Length = 485
Score = 89.0 bits (219), Expect(3) = 1e-24
Identities = 44/71 (61%), Positives = 51/71 (70%), Gaps = 6/71 (8%)
Frame = -3
Query: 27 VFLKSMDSIAWKAIVKGWKHPV------IASTTELKPEDKWTKKEDDEALGNSKALNVIF 80
VFLKS+D+ WKAIV GWKHP ++T KPE++WT ED+ ALGNSKALN IF
Sbjct: 243 VFLKSIDNKTWKAIVNGWKHPTKSQVEGTSTTVVEKPEEEWTSVEDEAALGNSKALNAIF 64
Query: 81 NGVDKNMFRLI 91
GVD NMFRLI
Sbjct: 63 YGVDXNMFRLI 31
Score = 39.3 bits (90), Expect(3) = 1e-24
Identities = 15/19 (78%), Positives = 16/19 (83%)
Frame = -2
Query: 6 SIYMPPILDGTNYDYWKAR 24
S+ PPILDGTNYDYWK R
Sbjct: 304 SVNRPPILDGTNYDYWKVR 248
Score = 22.3 bits (46), Expect(3) = 1e-24
Identities = 7/10 (70%), Positives = 10/10 (100%)
Frame = -2
Query: 92 NTCTVAKEAW 101
++CTVAK+AW
Sbjct: 31 HSCTVAKDAW 2
>TC10133 similar to UP|Q9LSS5 (Q9LSS5) Myosin heavy chain-like, partial
(34%)
Length = 1827
Score = 38.9 bits (89), Expect = 0.003
Identities = 67/326 (20%), Positives = 150/326 (45%), Gaps = 32/326 (9%)
Frame = +1
Query: 111 TSKVRMSKLQLLTTQFETMKMNEDESIYEFHMRIRDLANSTFALGEPMSEEKLARKILRS 170
T + R+++LQ +T + + +E E+ + L+ + AL +E +L + L
Sbjct: 232 TEEARITELQKITEECNRTRQSELEASQ------KHLSVDSAALASAANEIQLLKVQLEL 393
Query: 171 LPKRFDMKVTAIEEAQ-DISNIK--VDELIGSLQTFEMSLNGRSEKKAKSITFVSNTEED 227
+ ++ E A ++ N+K + E + ++ + L E +A++ VS T
Sbjct: 394 VANCESVQTHHAESADVELLNLKQNLSETLSLVENMKNQLRNFKEFEAQAQGLVSETLLQ 573
Query: 228 EDQREKDTD---ANIAEAV----SLLNKALKSLGRMSNTNVL------DNVSDNVKNTEF 274
+ ++ + ++A++V S+ + +S +++ VL D +++N N+
Sbjct: 574 LETAKRTVEILRGDVAKSVDGYKSIALELDQSRAKVNTLEVLVEKLNPDFINNNCNNSG- 750
Query: 275 QLKDKHENDTTKA--IHVKALIGKCYSDAESSD--GDEEELVETYKLLLAKWEESCMYGE 330
L D H +D +A +K+ +G+ S E+++ EE++ T ++ A + E
Sbjct: 751 DLVDGHRSDHVEAEICSLKSDVGRLRSAVETAEIKYQEEQIRSTVQIRNAYELMEQIKSE 930
Query: 331 KMRKEVK---DLIAEKKQLQSNNSSLQEEVKTISKLREENE----KLQI-----TNAKLQ 378
+KE + +L +K ++ ++L ++ + + EENE KLQ+ ++ +L+
Sbjct: 931 SGQKECEFEAELKRKKADIEELKANLMDKETELQGIMEENENLNSKLQVRTSCQSDHELR 1110
Query: 379 EEVTLLNSKLEGMKKSIRMMNKSTNV 404
E+ L + +K MM+K T +
Sbjct: 1111NEIKRLEHCVAALKAD--MMDKETTL 1182
>TC11361
Length = 549
Score = 38.1 bits (87), Expect = 0.005
Identities = 14/38 (36%), Positives = 22/38 (57%)
Frame = +3
Query: 459 RKSKKSTWRCHHCGKLGHIRPYCYKLYGYPQSHDQPRT 496
+ KK C HCG LGH + CY+L G+P + + ++
Sbjct: 15 KSHKKDRPTCTHCGILGHTKDKCYRLIGFPPHYKKSKS 128
>TC13132 similar to UP|Q9FJA1 (Q9FJA1) Similarity to retroelement pol
polyprotein, partial (3%)
Length = 591
Score = 35.8 bits (81), Expect = 0.023
Identities = 17/42 (40%), Positives = 21/42 (49%)
Frame = +1
Query: 462 KKSTWRCHHCGKLGHIRPYCYKLYGYPQSHDQPRTNPQVAPT 503
KK C HCG GH C+KL+GYP P P+ P+
Sbjct: 22 KKDRPICSHCGIPGHTAAKCFKLHGYP-----PGMKPKYKPS 132
>BP030371
Length = 516
Score = 32.7 bits (73), Expect = 0.19
Identities = 16/69 (23%), Positives = 36/69 (51%)
Frame = -2
Query: 136 SIYEFHMRIRDLANSTFALGEPMSEEKLARKILRSLPKRFDMKVTAIEEAQDISNIKVDE 195
++ E+ M I+ + + ++G+P+S ++ A IL P +++ V I + + +DE
Sbjct: 512 TVAEYLM*IKSITDPPRSIGDPVSAKEQAEIILEGFPTKYESLVNLIHMSDKFQALSLDE 333
Query: 196 LIGSLQTFE 204
+ L+ E
Sbjct: 332 IESLLRDQE 306
>BP037078
Length = 529
Score = 32.0 bits (71), Expect = 0.33
Identities = 31/132 (23%), Positives = 61/132 (45%)
Frame = +1
Query: 262 LDNVSDNVKNTEFQLKDKHENDTTKAIHVKALIGKCYSDAESSDGDEEELVETYKLLLAK 321
LD ++ + E Q+KDK + K + ++ E D+ VE+ + +A
Sbjct: 160 LDQLNSRILTLESQIKDKSQELKKK--------DEVIAEKEKFIQDKSSTVESLQNEVAS 315
Query: 322 WEESCMYGEKMRKEVKDLIAEKKQLQSNNSSLQEEVKTISKLREENEKLQITNAKLQEEV 381
++ + +EV A +LQ +L+ E++ K +E + A+L++++
Sbjct: 316 LQKKGSLDAQ--EEVGKAHARAGELQKQVENLKRELE---KQNKEKVNWETRVAELEKKI 480
Query: 382 TLLNSKLEGMKK 393
LNSKLE ++K
Sbjct: 481 HDLNSKLEDIQK 516
>TC11021
Length = 509
Score = 32.0 bits (71), Expect = 0.33
Identities = 21/62 (33%), Positives = 31/62 (49%)
Frame = +3
Query: 330 EKMRKEVKDLIAEKKQLQSNNSSLQEEVKTISKLREENEKLQITNAKLQEEVTLLNSKLE 389
E + KEV+ L K L N E K I L+++NE+L +L EE+ + S+ E
Sbjct: 45 ENLEKEVRSL---KLNLSFMNRKDSEXTKQIEDLQKQNEELADEKERLLEEIERILSETE 215
Query: 390 GM 391
M
Sbjct: 216 KM 221
>TC9039
Length = 1218
Score = 31.2 bits (69), Expect = 0.56
Identities = 13/23 (56%), Positives = 15/23 (64%)
Frame = +1
Query: 11 PILDGTNYDYWKARMMVFLKSMD 33
PILDGTN+ WK M + L MD
Sbjct: 154 PILDGTNFKDWKENMEIVLGCMD 222
>TC12218
Length = 737
Score = 30.4 bits (67), Expect = 0.96
Identities = 20/77 (25%), Positives = 42/77 (53%), Gaps = 1/77 (1%)
Frame = +2
Query: 335 EVKDLIAEKKQLQSNNSSL-QEEVKTISKLREENEKLQITNAKLQEEVTLLNSKLEGMKK 393
E+ ++ +Q +++ +++ + V+ +++LR E EKL+ N LQE++ +L+ K
Sbjct: 329 ELSSILEPGRQPKTDKAAIISDAVRVVTQLRNEAEKLKEMNNDLQEKI----KELKAEKN 496
Query: 394 SIRMMNKSTNVLEEILE 410
IR + +E LE
Sbjct: 497 EIRDEKNKLKLDKEKLE 547
>TC8301
Length = 1243
Score = 29.6 bits (65), Expect = 1.6
Identities = 24/99 (24%), Positives = 39/99 (39%)
Frame = +2
Query: 11 PILDGTNYDYWKARMMVFLKSMDSIAWKAIVKGWKHPVIASTTELKPEDKWTKKEDDEAL 70
P G+N+ WK ++ L+ + + P I +DKW K+ DD A+
Sbjct: 521 PKFSGSNFSLWKLKIKAILRKDNCL-----------PAIDGRPADITDDKW-KEMDDNAV 664
Query: 71 GNSKALNVIFNGVDKNMFRLINTCTVAKEAWEILKTAHE 109
N + V ++ I AKE W+ L +E
Sbjct: 665 AN------LHLAVADSVLSSIAEKKTAKEIWDTLIQLYE 763
>AV772411
Length = 443
Score = 28.5 bits (62), Expect = 3.6
Identities = 10/16 (62%), Positives = 13/16 (80%)
Frame = +2
Query: 451 VRHVYPQFRKSKKSTW 466
VR++ P+FRK KK TW
Sbjct: 188 VRYIRPKFRKGKKKTW 235
>CB827128
Length = 556
Score = 28.5 bits (62), Expect = 3.6
Identities = 30/129 (23%), Positives = 62/129 (47%), Gaps = 10/129 (7%)
Frame = +1
Query: 291 KALIGKCYSDAESSDGDEE--ELVETYKLLLAKWEESCMYGEKMRKEVKDLIAEKKQLQS 348
+AL +AE+ D +E+ L KL + E+ E + +++ + + K L++
Sbjct: 169 EALQRHAEKEAEAKDLNEKLNALEGQIKLHEEQAREAVAVSETHKSGLEESLLKLKHLET 348
Query: 349 NNSSLQEEV----KTISKLREENEKLQ----ITNAKLQEEVTLLNSKLEGMKKSIRMMNK 400
LQ ++ K + L EEN KL I +KL + + L++ L ++++ +
Sbjct: 349 VVDELQNKLLHHEKETAGLNEENTKLNQEIAIYESKLSDLQSKLSAALVEKDETVKEILT 528
Query: 401 STNVLEEIL 409
S + +EE++
Sbjct: 529 SKDAIEELV 555
>AI967308
Length = 443
Score = 28.1 bits (61), Expect = 4.8
Identities = 14/73 (19%), Positives = 36/73 (49%)
Frame = +1
Query: 336 VKDLIAEKKQLQSNNSSLQEEVKTISKLREENEKLQITNAKLQEEVTLLNSKLEGMKKSI 395
V ++ + ++ +L+E+V + + +ENE L+ + +V+ +K EGMK +
Sbjct: 193 VDEMTLKNDEIALLKCNLEEKVTELESMSKENENLKQQLNEAVSKVSEAETKEEGMKLQL 372
Query: 396 RMMNKSTNVLEEI 408
+ + + ++
Sbjct: 373 KELGEELEAASKL 411
>BP065323
Length = 446
Score = 27.7 bits (60), Expect = 6.2
Identities = 18/46 (39%), Positives = 23/46 (49%)
Frame = -1
Query: 574 GESVSADPNAIVPSVIHEISVESVSVPNVEPHVDTLVETTSDVSMG 619
GE A PNAI+ I +S+ EP++D V SDVS G
Sbjct: 440 GEKPDAIPNAIL------ILERLISMSVAEPNIDFHVRDKSDVSFG 321
>BP069559
Length = 456
Score = 27.7 bits (60), Expect = 6.2
Identities = 17/62 (27%), Positives = 28/62 (44%), Gaps = 4/62 (6%)
Frame = -1
Query: 337 KDLIAEKKQLQSNNSSLQEEVKTISKLREENEKL----QITNAKLQEEVTLLNSKLEGMK 392
+DL K+ Q+++ + E ISK E K+ + AK + + L KLE +
Sbjct: 450 EDLQRSSKEAQASSLDIAESTLDISKFHREEAKIIAWESLQKAKAEAAIRKLEMKLEKKR 271
Query: 393 KS 394
S
Sbjct: 270 SS 265
>BP028499
Length = 491
Score = 27.3 bits (59), Expect = 8.1
Identities = 14/42 (33%), Positives = 22/42 (52%)
Frame = +3
Query: 593 SVESVSVPNVEPHVDTLVETTSDVSMGPSVGNPNPIVDTSEL 634
S S S P+V P +S ++ PS+ +P+PI T+ L
Sbjct: 177 STSSASDPSVPPGATLPHPPSSPSTISPSISHPSPITITTTL 302
>TC9247 similar to UP|Q8L6A5 (Q8L6A5) Replication factor C large subunit
(Fragment), partial (10%)
Length = 884
Score = 27.3 bits (59), Expect = 8.1
Identities = 20/63 (31%), Positives = 33/63 (51%), Gaps = 5/63 (7%)
Frame = +1
Query: 346 LQSNNSSLQEEVKTISKLREEN----EKLQITNA-KLQEEVTLLNSKLEGMKKSIRMMNK 400
L+ N+ + Q + T+ + EEN E L T KLQ E+ LNSK ++ ++ K
Sbjct: 142 LEPNDEAEQVDGDTLDESEEENTSDTEDLGTTTGEKLQSELQSLNSKATQVQLELKGTEK 321
Query: 401 STN 403
S++
Sbjct: 322 SSS 330
>TC8135 similar to UP|GUN4_ARATH (Q9LX31) Tetrapyrrole-binding protein,
chloroplast precursor (Genomes uncoulped 4), partial
(51%)
Length = 1213
Score = 27.3 bits (59), Expect = 8.1
Identities = 16/52 (30%), Positives = 29/52 (55%)
Frame = +2
Query: 613 TSDVSMGPSVGNPNPIVDTSELDLQIHQSTLGSEPSTVCKKSVVESVHELLS 664
TS +++ S+ P+P S + + Q+T + PST K + ++ + ELLS
Sbjct: 230 TSSITLSNSL-LPSP-TSNSSVTFSVSQTTSSTTPSTTSKSTSLDLLRELLS 379
>TC8647 similar to PIR|S32145|S32145 anther-specific protein - rape
(fragment) {Brassica napus;} , partial (76%)
Length = 759
Score = 27.3 bits (59), Expect = 8.1
Identities = 11/34 (32%), Positives = 19/34 (55%)
Frame = -2
Query: 437 QTKQPMSDPMLHHSVRHVYPQFRKSKKSTWRCHH 470
Q ++P+ +P+LHH + P + S K + C H
Sbjct: 413 QVQKPVPNPILHHHLPSETPSYTPSHKI*YYC*H 312
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.311 0.127 0.353
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,029,695
Number of Sequences: 28460
Number of extensions: 126253
Number of successful extensions: 692
Number of sequences better than 10.0: 42
Number of HSP's better than 10.0 without gapping: 684
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 690
length of query: 667
length of database: 4,897,600
effective HSP length: 96
effective length of query: 571
effective length of database: 2,165,440
effective search space: 1236466240
effective search space used: 1236466240
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 58 (26.9 bits)
Lotus: description of TM0075.5