BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0069.1
(367 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC14009 similar to UP|HSF5_ARATH (Q9S7U5) Heat shock factor prot... 256 3e-69
TC19060 similar to UP|Q9SXK9 (Q9SXK9) Heat shock factor, partial... 129 8e-31
TC13752 similar to GB|CAB63803.1|6624618|ATH251868 heat shock fa... 117 2e-27
AV415736 94 4e-20
TC12119 68 3e-12
AV423559 60 6e-10
BP056960 55 2e-08
TC19063 similar to UP|O23259 (O23259) Heat shock transcription f... 32 0.13
TC19330 similar to UP|Q96258 (Q96258) AR791, partial (11%) 32 0.22
TC15958 similar to GB|AAP37838.1|30725632|BT008479 At4g24220 {Ar... 30 0.83
BI419806 28 1.8
TC9130 homologue to UP|Q8LLE2 (Q8LLE2) BEL1-related homeotic pro... 28 2.4
AV428128 28 3.1
TC15304 28 3.1
CN825616 27 4.1
TC15257 similar to PIR|T39903|T39903 serine-rich protein - fissi... 27 4.1
CB829481 27 5.4
TC7848 homologue to UP|Q9AVR8 (Q9AVR8) Sucrose synthase isoform ... 27 5.4
BI419956 27 7.0
TC17878 similar to GB|AAK91488.1|15215889|AY050475 AT5g02770/F9G... 27 7.0
>TC14009 similar to UP|HSF5_ARATH (Q9S7U5) Heat shock factor protein 5 (HSF
5) (Heat shock transcription factor 5) (HSTF 5), partial
(27%)
Length = 406
Score = 256 bits (655), Expect = 3e-69
Identities = 123/123 (100%), Positives = 123/123 (100%)
Frame = +1
Query: 1 MVKSKENASGSSSVAPFLNKCYDMVEDDSTDSIISWTEPSGHTFVISDITAFSVTLLPTY 60
MVKSKENASGSSSVAPFLNKCYDMVEDDSTDSIISWTEPSGHTFVISDITAFSVTLLPTY
Sbjct: 37 MVKSKENASGSSSVAPFLNKCYDMVEDDSTDSIISWTEPSGHTFVISDITAFSVTLLPTY 216
Query: 61 FKHNNFASFIRQLNIYGFRKVDTDRWEFANENFVRGQKHLLKNIRRRKHPHVTDQQKALP 120
FKHNNFASFIRQLNIYGFRKVDTDRWEFANENFVRGQKHLLKNIRRRKHPHVTDQQKALP
Sbjct: 217 FKHNNFASFIRQLNIYGFRKVDTDRWEFANENFVRGQKHLLKNIRRRKHPHVTDQQKALP 396
Query: 121 EHN 123
EHN
Sbjct: 397 EHN 405
>TC19060 similar to UP|Q9SXK9 (Q9SXK9) Heat shock factor, partial (58%)
Length = 660
Score = 129 bits (324), Expect = 8e-31
Identities = 80/203 (39%), Positives = 113/203 (55%), Gaps = 17/203 (8%)
Frame = +1
Query: 5 KENASGSSSVAPFLNKCYDMVEDDSTDSIISWTEPSGHTFVISDITAFSVTLLPTYFKHN 64
+E S S APFL K Y+MV+D +T+ +ISW+E SG+TFV+ F+ LLP+YFKHN
Sbjct: 10 EEKMSQRSVPAPFLTKTYEMVDDPATEDVISWSE-SGNTFVVWKHADFAKDLLPSYFKHN 186
Query: 65 NFASFIRQLNIYGFRKVDTDRWEFANENFVRGQKHLLKNIRRRKHPHVTDQQKALPE--- 121
NF+SF+RQLN YGFRK+ D+WEFANENF RG++ LL I+RRK V PE
Sbjct: 187 NFSSFVRQLNTYGFRKIVPDKWEFANENFKRGKRELLSEIKRRK-TVVAQPSAQAPEVGK 363
Query: 122 ------HNNSDE-----PSREAPNHGLRKEVENLKSDRNSL---MQELVHLSQHLESAES 167
++ DE S +P VE + N+L + +LS E +
Sbjct: 364 SGNSTSNSGGDEVGSSSTSTSSPGSKNPGSVETTTAPPNTLPPATLQCANLSSENEKLKK 543
Query: 168 KMLVLSDRLQGMEKHQQQMLSFL 190
+ L L +K +++++FL
Sbjct: 544 ENETLGWELARAKKQCEELVAFL 612
>TC13752 similar to GB|CAB63803.1|6624618|ATH251868 heat shock factor 7
{Arabidopsis thaliana;} , partial (36%)
Length = 501
Score = 117 bits (294), Expect = 2e-27
Identities = 55/93 (59%), Positives = 70/93 (75%)
Frame = +3
Query: 16 PFLNKCYDMVEDDSTDSIISWTEPSGHTFVISDITAFSVTLLPTYFKHNNFASFIRQLNI 75
PFL K Y +V+D S D +ISW + G++F++ F+ LLP YFKHNNF+SF+RQLN
Sbjct: 75 PFLTKTYQLVDDPSVDDLISWNK-DGNSFIVWRPAEFARDLLPKYFKHNNFSSFVRQLNT 251
Query: 76 YGFRKVDTDRWEFANENFVRGQKHLLKNIRRRK 108
YGFRKV DRWEFAN+ F RGQK LL++I+RRK
Sbjct: 252 YGFRKVVPDRWEFANDGFRRGQKDLLRDIQRRK 350
>AV415736
Length = 214
Score = 94.0 bits (232), Expect = 4e-20
Identities = 43/72 (59%), Positives = 54/72 (74%)
Frame = +2
Query: 27 DDSTDSIISWTEPSGHTFVISDITAFSVTLLPTYFKHNNFASFIRQLNIYGFRKVDTDRW 86
D S D +ISW + G TF++ + T F+ LLP YFKHNNF+SF+RQLN YGFRKV DRW
Sbjct: 2 DQSIDDVISWND-DGSTFIVWNPTVFARDLLPKYFKHNNFSSFVRQLNTYGFRKVIPDRW 178
Query: 87 EFANENFVRGQK 98
EF+NE F +G+K
Sbjct: 179EFSNEFFRKGEK 214
>TC12119
Length = 718
Score = 67.8 bits (164), Expect = 3e-12
Identities = 32/32 (100%), Positives = 32/32 (100%)
Frame = +3
Query: 336 GGMEMEATIPETPNEHSRIFESMIMEMEKTQV 367
GGMEMEATIPETPNEHSRIFESMIMEMEKTQV
Sbjct: 3 GGMEMEATIPETPNEHSRIFESMIMEMEKTQV 98
>AV423559
Length = 448
Score = 60.1 bits (144), Expect = 6e-10
Identities = 32/73 (43%), Positives = 44/73 (59%)
Frame = +3
Query: 140 EVENLKSDRNSLMQELVHLSQHLESAESKMLVLSDRLQGMEKHQQQMLSFLVMVVQSPGF 199
EV+ L+ DR LM ELV L Q +S + + + R++ E+ QQQM+SFL VQ+P F
Sbjct: 6 EVDRLRRDRQVLMVELVKLRQQQQSTRAHIQAMEGRIKRTEQKQQQMMSFLARAVQNPNF 185
Query: 200 MVQLLHPKENSWR 212
+ QL KE WR
Sbjct: 186 LQQLAQQKE--WR 218
>BP056960
Length = 511
Score = 55.1 bits (131), Expect = 2e-08
Identities = 22/31 (70%), Positives = 28/31 (89%)
Frame = -2
Query: 282 LKVLLDEKVSPLDNHSPFLLPDLPDDGSWEQ 312
+++ +DEKV PL+NHSP LLPDLP+DGSWEQ
Sbjct: 510 VRMCMDEKVCPLNNHSPLLLPDLPNDGSWEQ 418
>TC19063 similar to UP|O23259 (O23259) Heat shock transcription factor
homolog (Heat shock transcription factor like protein),
partial (14%)
Length = 492
Score = 32.3 bits (72), Expect = 0.13
Identities = 14/29 (48%), Positives = 20/29 (68%)
Frame = +3
Query: 175 RLQGMEKHQQQMLSFLVMVVQSPGFMVQL 203
RL GMEK Q+Q+L+F +Q+P F+ L
Sbjct: 15 RLDGMEKRQKQLLNFFEKALQNPTFVEHL 101
>TC19330 similar to UP|Q96258 (Q96258) AR791, partial (11%)
Length = 507
Score = 31.6 bits (70), Expect = 0.22
Identities = 15/25 (60%), Positives = 20/25 (80%)
Frame = +2
Query: 134 NHGLRKEVENLKSDRNSLMQELVHL 158
N GL KEVE L++DR S ++ELV+L
Sbjct: 341 NEGLTKEVEQLQADRCSDVEELVYL 415
>TC15958 similar to GB|AAP37838.1|30725632|BT008479 At4g24220 {Arabidopsis
thaliana;}, partial (52%)
Length = 800
Score = 29.6 bits (65), Expect = 0.83
Identities = 16/54 (29%), Positives = 26/54 (47%), Gaps = 7/54 (12%)
Frame = +2
Query: 224 KEDDKPVASDGMIVQYKPPVGEKRKHVIPIPLSP-------GFDRQPEPELSAD 270
+E +P S G+I+ VG ++P+P +P G R+P P +AD
Sbjct: 221 EEPPRPFTSVGLIIGVTGIVGNSLAEILPLPDTPGGRWKVYGVARRPRPSWNAD 382
>BI419806
Length = 448
Score = 28.5 bits (62), Expect = 1.8
Identities = 23/88 (26%), Positives = 39/88 (44%), Gaps = 6/88 (6%)
Frame = +3
Query: 67 ASFIRQ-----LNIYGFRKVDTDRWEFANENFVRGQKHLLKNIRRRKHPHVTDQQKALPE 121
A F+R +N YG + R E VRGQ+ L++ +R + K L E
Sbjct: 141 AQFVRDPLDMDVNEYGRALSPSSR----TEAIVRGQRELMEMVRNMPESNYELSLKDLVE 308
Query: 122 HNN-SDEPSREAPNHGLRKEVENLKSDR 148
H + D P + + K++++L+ R
Sbjct: 309 HQHWRDAPQENSEENEEEKKIKSLQEAR 392
>TC9130 homologue to UP|Q8LLE2 (Q8LLE2) BEL1-related homeotic protein 13
(Fragment), partial (5%)
Length = 728
Score = 28.1 bits (61), Expect = 2.4
Identities = 11/22 (50%), Positives = 17/22 (77%)
Frame = +1
Query: 7 NASGSSSVAPFLNKCYDMVEDD 28
NA+ +S VAP +++C+D V DD
Sbjct: 76 NATTASEVAPPVSQCFDSVSDD 141
>AV428128
Length = 415
Score = 27.7 bits (60), Expect = 3.1
Identities = 17/59 (28%), Positives = 29/59 (48%), Gaps = 1/59 (1%)
Frame = -1
Query: 283 KVLLDEKVSPLDNHSPFLLPDLPDDGSWEQLFLGSPFLENIEDTD-HENEEPTVGGMEM 340
+ L E +S + H+P L ++ SW+ GS ++ D+D E E ++G EM
Sbjct: 235 RALARETLSMTERHAPQLARSWREERSWQSSSNGSRRRGSVWDSDLGEGESESIGRSEM 59
>TC15304
Length = 477
Score = 27.7 bits (60), Expect = 3.1
Identities = 16/51 (31%), Positives = 22/51 (42%)
Frame = -1
Query: 100 LLKNIRRRKHPHVTDQQKALPEHNNSDEPSREAPNHGLRKEVENLKSDRNS 150
LL++I R +H V Q+ L P PNHG K+ N + S
Sbjct: 291 LLQSIERSEHVGVVQHQRTLV-------PPASTPNHGYLKKTRNESGNSRS 160
>CN825616
Length = 586
Score = 27.3 bits (59), Expect = 4.1
Identities = 17/46 (36%), Positives = 25/46 (53%)
Frame = -1
Query: 101 LKNIRRRKHPHVTDQQKALPEHNNSDEPSREAPNHGLRKEVENLKS 146
LK RK HV Q+++ P+HN +P+R+ + VE LKS
Sbjct: 358 LKQSFFRKIAHV--QERSYPDHNQMPDPTRK-DAYAYEPRVETLKS 230
>TC15257 similar to PIR|T39903|T39903 serine-rich protein - fission yeast
(Schizosaccharomyces pombe)
{Schizosaccharomyces pombe;}, partial (5%)
Length = 572
Score = 27.3 bits (59), Expect = 4.1
Identities = 15/61 (24%), Positives = 26/61 (42%)
Frame = +2
Query: 101 LKNIRRRKHPHVTDQQKALPEHNNSDEPSREAPNHGLRKEVENLKSDRNSLMQELVHLSQ 160
L++++R H ++ P H NS+ PS P H L+ D+ +H +
Sbjct: 119 LRSLQRILQDHPLMEKNLQPNHPNSNPPSHLYPPHPHGSLQHPLQQDQQQHTGNALHTTG 298
Query: 161 H 161
H
Sbjct: 299 H 301
>CB829481
Length = 576
Score = 26.9 bits (58), Expect = 5.4
Identities = 14/41 (34%), Positives = 23/41 (55%), Gaps = 3/41 (7%)
Frame = +3
Query: 129 SREAPNHGLRKEVENLKSDRNSLMQEL---VHLSQHLESAE 166
S + +H LRK+ +++ RNSL+ + + L QHL E
Sbjct: 159 SIKTQHHSLRKQSNSMRVSRNSLLARVMFWIFLHQHLTRLE 281
>TC7848 homologue to UP|Q9AVR8 (Q9AVR8) Sucrose synthase isoform 3 ,
complete
Length = 2779
Score = 26.9 bits (58), Expect = 5.4
Identities = 15/53 (28%), Positives = 27/53 (50%)
Frame = +1
Query: 135 HGLRKEVENLKSDRNSLMQELVHLSQHLESAESKMLVLSDRLQGMEKHQQQML 187
+G K V NL N E+ + ++ + AES L + + QGM+ +++ L
Sbjct: 2374 YGFWKHVTNLDRRENKRYLEMFYALKYSQLAESVPLAVEE*NQGMQLSEEKRL 2532
>BI419956
Length = 495
Score = 26.6 bits (57), Expect = 7.0
Identities = 10/24 (41%), Positives = 13/24 (53%)
Frame = +3
Query: 105 RRRKHPHVTDQQKALPEHNNSDEP 128
RRR+H H D A P N ++P
Sbjct: 54 RRREHAHQMDSALATPPRNGGEQP 125
>TC17878 similar to GB|AAK91488.1|15215889|AY050475 AT5g02770/F9G14_80
{Arabidopsis thaliana;}, partial (17%)
Length = 465
Score = 26.6 bits (57), Expect = 7.0
Identities = 16/59 (27%), Positives = 29/59 (49%), Gaps = 1/59 (1%)
Frame = +2
Query: 213 LAEAGNMFDPGKED-DKPVASDGMIVQYKPPVGEKRKHVIPIPLSPGFDRQPEPELSAD 270
+A + N P +E+ +K + + + PP E + PI P + +PEPE ++D
Sbjct: 65 MASSPNDTTPTQENPNKTLEPEEEAEEPTPPQSEPQSQPDPIAAEPETEPEPEPEPASD 241
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.315 0.133 0.387
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,437,612
Number of Sequences: 28460
Number of extensions: 94273
Number of successful extensions: 373
Number of sequences better than 10.0: 41
Number of HSP's better than 10.0 without gapping: 366
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 371
length of query: 367
length of database: 4,897,600
effective HSP length: 92
effective length of query: 275
effective length of database: 2,279,280
effective search space: 626802000
effective search space used: 626802000
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 56 (26.2 bits)
Lotus: description of TM0069.1