BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0065.20
(196 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV766088 67 2e-12
BP063694 65 6e-12
BP033602 59 7e-10
AV764934 40 3e-04
BP063902 40 3e-04
BP058111 40 3e-04
BP078157 40 4e-04
BP061842 39 6e-04
TC9039 36 0.004
BP081717 34 0.015
AV406379 33 0.025
TC9337 similar to UP|PME_PRUPE (Q43062) Pectinesterase PPE8B pre... 31 0.16
BP073434 29 0.62
BP054557 27 1.8
BP030995 27 3.1
TC11719 27 3.1
BP035453 25 6.9
TC12784 similar to UP|Q850H6 (Q850H6) Gag-pol polyprotein (Fragm... 25 6.9
BP037555 25 9.0
AV418055 25 9.0
>AV766088
Length = 501
Score = 67.4 bits (163), Expect = 2e-12
Identities = 37/107 (34%), Positives = 58/107 (53%)
Frame = -3
Query: 77 SSSIWHQRLGHMSEKGMKIMASKGKMSNLKHVDLGVCEHCILGKQRKVSFSKAGRKSKLE 136
+ S+WH RLGH S ++ + S +S VCE C+ GK ++ F + + +
Sbjct: 316 AQSLWHSRLGHPSSSALRYLRSNKFISYELLNYSPVCESCVFGKHVRLPFVSSNNVTVMP 137
Query: 137 KLKLVHTDVWGPAPVKSLGGSRYYVTFIDDSTRKV*VYFLKNKSDVF 183
++H+D+W +PV S G R+YV F+DD T + + L NKS VF
Sbjct: 136 -FDILHSDLW-TSPVLSSAGHRFYVLFLDDFTDFLWTFPLSNKSQVF 2
>BP063694
Length = 511
Score = 65.5 bits (158), Expect = 6e-12
Identities = 42/145 (28%), Positives = 72/145 (48%), Gaps = 5/145 (3%)
Frame = -2
Query: 46 KGNLVVARGKKRGSLYMVAEEDMIAVTEAVN----SSSIWHQRLGHMSEKGMKIMASKGK 101
K V+ R + LY++ ++ +T + S +WH RLGH++ +K + G
Sbjct: 474 KTGAVLGRXRCDQGLYVLNQDSQALLTTSSPLPRASFELWHSRLGHVNFDIIKQLHKHGC 295
Query: 102 MSNLKHVDLGVC-EHCILGKQRKVSFSKAGRKSKLEKLKLVHTDVWGPAPVKSLGGSRYY 160
+ + +C C + K +++ F +++ L L+H D+ GP+PV S+ G Y+
Sbjct: 294 LDVSSILPKPICCTSCQMAKSKRLVFHDNNKRASAV-LDLIHCDLRGPSPVASIDGFSYF 118
Query: 161 VTFIDDSTRKV*VYFLKNKSDVFSV 185
V F+DD +R Y LK KSD V
Sbjct: 117 VIFVDDFSRFTWFYPLKRKSDFSDV 43
>BP033602
Length = 533
Score = 58.5 bits (140), Expect = 7e-10
Identities = 34/102 (33%), Positives = 56/102 (54%), Gaps = 2/102 (1%)
Frame = +3
Query: 69 IAVTEAVNSSSIWHQRLGHMSEKGMK-IMASKGKMSNLKHVDLGVCEHCILGK-QRKVSF 126
++ T + +WH RLGH + + ++ + K N ++ CE C+L K QR +
Sbjct: 243 MSCTSVRDQIMLWHNRLGHPNFQYLRHLFPDLFKNVNCSSLE---CESCVLAKNQRAPYY 413
Query: 127 SKAGRKSKLEKLKLVHTDVWGPAPVKSLGGSRYYVTFIDDST 168
S+ S+ L+H+DVWGP+ + + G R++VTFIDD T
Sbjct: 414 SQPYHASR--PFYLIHSDVWGPSKITTQFGKRWFVTFIDDHT 533
>AV764934
Length = 406
Score = 40.0 bits (92), Expect = 3e-04
Identities = 25/75 (33%), Positives = 38/75 (50%)
Frame = -2
Query: 79 SIWHQRLGHMSEKGMKIMASKGKMSNLKHVDLGVCEHCILGKQRKVSFSKAGRKSKLEKL 138
S WH RLGH +K + S +S + C LGK ++ S+ + L
Sbjct: 219 STWHSRLGHPHLDNLKRVLSSCNVSYSPKDTVEFRTACCLGKAHRLP-SQMSTTTYLP-F 46
Query: 139 KLVHTDVWGPAPVKS 153
+L+++D+WGPAPV S
Sbjct: 45 ELIYSDLWGPAPVCS 1
>BP063902
Length = 494
Score = 40.0 bits (92), Expect = 3e-04
Identities = 23/84 (27%), Positives = 37/84 (43%)
Frame = -3
Query: 46 KGNLVVARGKKRGSLYMVAEEDMIAVTEAVNSSSIWHQRLGHMSEKGMKIMASKGKMSNL 105
K + + R G LY V A +SS+WH RLGH + + + + +
Sbjct: 273 KTGMPLLRCNSLGDLYPVTRSSPFAGL----ASSVWHHRLGHPASSALNHLRNNKLIFCE 106
Query: 106 KHVDLGVCEHCILGKQRKVSFSKA 129
VC+ C+LGK ++ FS +
Sbjct: 105 PSRSSSVCDSCVLGKHVRLPFSSS 34
>BP058111
Length = 570
Score = 40.0 bits (92), Expect = 3e-04
Identities = 22/65 (33%), Positives = 33/65 (49%)
Frame = -1
Query: 79 SIWHQRLGHMSEKGMKIMASKGKMSNLKHVDLGVCEHCILGKQRKVSFSKAGRKSKLEKL 138
++WH RLGH S K + I+ K + C+ C + KQ+K+SF + S E
Sbjct: 189 NLWHLRLGHTSSKKLAILQKNFPFITCKKI-TSPCDTCHMAKQKKLSFPNSVTLSS-EIF 16
Query: 139 KLVHT 143
L+HT
Sbjct: 15 DLIHT 1
>BP078157
Length = 395
Score = 39.7 bits (91), Expect = 4e-04
Identities = 17/47 (36%), Positives = 28/47 (59%)
Frame = -2
Query: 80 IWHQRLGHMSEKGMKIMASKGKMSNLKHVDLGVCEHCILGKQRKVSF 126
+WH RLGH+S+K +K++++ + L C C L K R++SF
Sbjct: 148 LWHSRLGHLSDKVLKVVSNLVPFTVLPDFHSHNCNVCPLSKMRRLSF 8
>BP061842
Length = 318
Score = 38.9 bits (89), Expect = 6e-04
Identities = 15/28 (53%), Positives = 21/28 (74%)
Frame = -3
Query: 138 LKLVHTDVWGPAPVKSLGGSRYYVTFID 165
L+LV D+WGPAP S G +YY++F+D
Sbjct: 88 LELVPCDLWGPAPCVSTNGFKYYLSFVD 5
>TC9039
Length = 1218
Score = 36.2 bits (82), Expect = 0.004
Identities = 18/52 (34%), Positives = 30/52 (57%), Gaps = 2/52 (3%)
Frame = +3
Query: 15 VPGIKRNLISIGQLDDEGYHTTFGGGAWKVTKGNLVVARGKKRG--SLYMVA 64
VP +RNLIS+ LD GY +FG +++ + +V G G +LY+++
Sbjct: 1062 VPSFRRNLISVSNLDKSGYSCSFGNSKAELSFNSNIVGTGSLIGYDNLYLLS 1217
>BP081717
Length = 291
Score = 34.3 bits (77), Expect = 0.015
Identities = 23/83 (27%), Positives = 41/83 (48%), Gaps = 7/83 (8%)
Frame = -1
Query: 59 SLYMVAEEDMIAVTEAVNSSS-------IWHQRLGHMSEKGMKIMASKGKMSNLKHVDLG 111
SL +++ + + E V + +WH RLGH S K ++ ++ L H ++
Sbjct: 252 SLIGISQISFVNIAETVPGKNGSHPQFDMWHFRLGHPSSKVLQHISKVFPYVKL-HSNI- 79
Query: 112 VCEHCILGKQRKVSFSKAGRKSK 134
+C+ C + KQ K+ F + KSK
Sbjct: 78 ICDFCHMAKQTKLPFPISDTKSK 10
>AV406379
Length = 226
Score = 33.5 bits (75), Expect = 0.025
Identities = 21/50 (42%), Positives = 29/50 (58%)
Frame = -3
Query: 77 SSSIWHQRLGHMSEKGMKIMASKGKMSNLKHVDLGVCEHCILGKQRKVSF 126
S + H RLGH S + +K M K +S+LK ++ CE C LGK + SF
Sbjct: 176 SPDLLHCRLGHPSLERLKQMVPK--LSHLKTLE---CESCQLGKHVRASF 42
>TC9337 similar to UP|PME_PRUPE (Q43062) Pectinesterase PPE8B precursor
(Pectin methylesterase) (PE) , partial (54%)
Length = 1145
Score = 30.8 bits (68), Expect = 0.16
Identities = 15/42 (35%), Positives = 25/42 (58%)
Frame = +3
Query: 112 VCEHCILGKQRKVSFSKAGRKSKLEKLKLVHTDVWGPAPVKS 153
VC+ C+LGK ++ FS + L ++H+D+W +PV S
Sbjct: 1026 VCDSCVLGKHVRLPFS-SSETITLRPFDILHSDLW-TSPVLS 1145
>BP073434
Length = 490
Score = 28.9 bits (63), Expect = 0.62
Identities = 14/49 (28%), Positives = 19/49 (38%)
Frame = -1
Query: 72 TEAVNSSSIWHQRLGHMSEKGMKIMASKGKMSNLKHVDLGVCEHCILGK 120
T N IWH RLGH + ++ + + C C LGK
Sbjct: 199 TTTTNKFLIWHSRLGHPHQDALQAVLQSCNIHLSSRDMTDFCYACCLGK 53
>BP054557
Length = 538
Score = 27.3 bits (59), Expect = 1.8
Identities = 12/38 (31%), Positives = 18/38 (46%)
Frame = -2
Query: 79 SIWHQRLGHMSEKGMKIMASKGKMSNLKHVDLGVCEHC 116
++WH RLGH S K + I+ K + C+ C
Sbjct: 120 NLWHLRLGHTSSKKLAILQKNFPFITCKQI-TSPCDTC 10
>BP030995
Length = 458
Score = 26.6 bits (57), Expect = 3.1
Identities = 9/14 (64%), Positives = 11/14 (78%)
Frame = -3
Query: 78 SSIWHQRLGHMSEK 91
S +WH RLGH S+K
Sbjct: 108 SRLWHSRLGHASQK 67
>TC11719
Length = 376
Score = 26.6 bits (57), Expect = 3.1
Identities = 9/14 (64%), Positives = 11/14 (78%)
Frame = +3
Query: 78 SSIWHQRLGHMSEK 91
S +WH RLGH S+K
Sbjct: 267 SRLWHSRLGHASQK 308
>BP035453
Length = 550
Score = 25.4 bits (54), Expect = 6.9
Identities = 20/78 (25%), Positives = 32/78 (40%), Gaps = 9/78 (11%)
Frame = -1
Query: 27 QLDDEGYHTTFGGGAWKVTKGNLVVARGKKRGSLYMVAEEDMIAVTEAVNSSS------- 79
++D + T G K KG ++ + + + + + +VN SS
Sbjct: 313 RMDSSAWRTI---GTVKAVKGLYILNKSSVTPLSHCNSVHSTVNIPSSVNVSSHFDPSVH 143
Query: 80 -IWHQRLGHMS-EKGMKI 95
+WH RLGH S KG I
Sbjct: 142 NLWHYRLGHPSFVKGQSI 89
>TC12784 similar to UP|Q850H6 (Q850H6) Gag-pol polyprotein (Fragment),
partial (15%)
Length = 460
Score = 25.4 bits (54), Expect = 6.9
Identities = 10/17 (58%), Positives = 11/17 (63%)
Frame = +3
Query: 143 TDVWGPAPVKSLGGSRY 159
+DVWGP PV S G Y
Sbjct: 333 SDVWGPCPVTSKLGYEY 383
>BP037555
Length = 549
Score = 25.0 bits (53), Expect = 9.0
Identities = 11/19 (57%), Positives = 14/19 (72%)
Frame = -2
Query: 145 VWGPAPVKSLGGSRYYVTF 163
VWGP PVK++ G RY + F
Sbjct: 503 VWGPLPVKNIIG-RYVMCF 450
>AV418055
Length = 407
Score = 25.0 bits (53), Expect = 9.0
Identities = 12/39 (30%), Positives = 18/39 (45%)
Frame = +1
Query: 130 GRKSKLEKLKLVHTDVWGPAPVKSLGGSRYYVTFIDDST 168
G ++ + + K + W P P S+ S V I DST
Sbjct: 4 GGEAVVNRCKALQLKHWAPIPASSVADSLLSVLHIGDST 120
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.320 0.136 0.407
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,361,139
Number of Sequences: 28460
Number of extensions: 42383
Number of successful extensions: 211
Number of sequences better than 10.0: 42
Number of HSP's better than 10.0 without gapping: 204
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 205
length of query: 196
length of database: 4,897,600
effective HSP length: 85
effective length of query: 111
effective length of database: 2,478,500
effective search space: 275113500
effective search space used: 275113500
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 53 (25.0 bits)
Lotus: description of TM0065.20