BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0065.18
(799 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC12832 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-rela... 151 4e-37
BP066094 111 3e-26
AV780009 105 3e-23
BP075943 84 9e-17
BP084332 80 1e-15
AV427921 80 2e-15
AU088980 71 8e-13
TC19389 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-rela... 69 4e-12
AV424544 62 5e-10
BP043850 60 1e-09
TC16929 weakly similar to UP|Q9LFY6 (Q9LFY6) T7N9.5, partial (4%) 59 2e-09
TC15664 weakly similar to UP|O81617 (O81617) F8M12.17 protein, p... 50 1e-06
BP057233 47 2e-05
BP052632 44 1e-04
BP084259 44 1e-04
TC10186 weakly similar to UP|Q9FH39 (Q9FH39) Copia-type polyprot... 32 7e-04
TC18909 30 0.015
BP037734 31 0.016
TC17272 34 0.10
TC14791 similar to UP|Q9LJ04 (Q9LJ04) ESTs AU082210(C53655), par... 33 0.14
>TC12832 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-related Pol
polyprotein from transposon TNT 1-94 [Contains: Protease
; Reverse transcriptase ; Endonuclease] , partial (9%)
Length = 747
Score = 151 bits (381), Expect = 4e-37
Identities = 76/149 (51%), Positives = 102/149 (68%), Gaps = 1/149 (0%)
Frame = +2
Query: 435 FNEFMSNSGFNRCDMDHCCFVKKFADS-YIILALYVDDMLIAGSNMTEINRLKQQMSENF 493
F+ F+ + G+NR DHC + K+F D+ +IIL LYVDDML+ G N + LK Q++ F
Sbjct: 2 FDSFIMSLGYNRLSSDHCTYHKRFDDNDFIILLLYVDDMLVVGPNKDRVQELKAQLAREF 181
Query: 494 EMKDLGPAKQILGMRISRNRSEGVLKLSQEKYVEKLLDRFNVGDANTRSTPLGNHLKFSK 553
+MKDLGPA +ILGM+I R+R + + LSQ+ Y++K+L RFN+ D N STPL + K S
Sbjct: 182 DMKDLGPANKILGMQIHRDRKDRRIWLSQKNYLQKVLRRFNMQDCNPISTPLPVNYKLSS 361
Query: 554 KQSPQTDEEESYMSTVPYASAVGSLMYAM 582
P ++ E MS VPYASAVGSLMYAM
Sbjct: 362 SMIPSSEAERMEMSRVPYASAVGSLMYAM 448
>BP066094
Length = 532
Score = 111 bits (277), Expect(2) = 3e-26
Identities = 55/148 (37%), Positives = 86/148 (57%)
Frame = +2
Query: 361 KMTTIRVILSIVAAENLHLEQLDVKIAFLHGDLEEEIYMTQPEGFEVLGTKNLVCKLHKS 420
K IR+++S N+ L Q++VK AFL+G + EE+Y+ QP G E + + KL KS
Sbjct: 89 KTEAIRLLISFSVNHNIILHQMNVKSAFLNGYISEEVYVHQPPGXEDEKNSDHIFKLKKS 268
Query: 421 LYGLKQAPRQWYKKFNEFMSNSGFNRCDMDHCCFVKKFADSYIILALYVDDMLIAGSNMT 480
LYGLKQAPR WY++ + F+ + R +D F K + D +I+ +YVDD++ +N +
Sbjct: 269 LYGLKQAPRAWYERLSSFLLENEXVRGKVDTTLFCKTYKDDILIVQIYVDDIIFGSANPS 448
Query: 481 EINRLKQQMSENFEMKDLGPAKQILGMR 508
+ M FEM+ +G K LG++
Sbjct: 449 LCKEFSEMMQAEFEMRMMGELKYFLGIQ 532
Score = 25.0 bits (53), Expect(2) = 3e-26
Identities = 9/18 (50%), Positives = 14/18 (77%)
Frame = +3
Query: 344 FQQKQGIDFTEIFSPVVK 361
+ Q+ GID+TE F+PV +
Sbjct: 39 YSQQ*GIDYTETFAPVAR 92
>AV780009
Length = 529
Score = 105 bits (262), Expect = 3e-23
Identities = 63/175 (36%), Positives = 93/175 (53%), Gaps = 2/175 (1%)
Frame = -1
Query: 614 ILRYLKGSSRMCLCFRRNN-LTLQEFSDADLGGDSDGGKSTTGYIFTLGGTAVSWKSKLQ 672
+LRY+KG+ L F ++ L LQ +SD+D G D +S TGY LG + +SW++K Q
Sbjct: 514 VLRYVKGAPAQGLFFSADSPLKLQAYSDSDWAGCPDTRRSVTGYSIFLGTSLISWRTKKQ 335
Query: 673 NRVALSTTESEYVAISEAAKEMIWLKSFLKELGKEQDVP-PLFSDSQSVIFLAKNPVFHS 731
V+ S++E+EY A++ E+ WL + L +P PLF D+QS + +A NP FH
Sbjct: 334 TTVSRSSSEAEYRALAATVCEVQWLSYLFQFLKLNVPLPVPLFCDNQSALHIAHNPTFHE 155
Query: 732 RCKHIQMKYHFIRELISDEELSLLKILGSENPTDMLTKTVTADNLRLCIASAGLR 786
R KHI++ H +R + + LL I D+ TK LC G R
Sbjct: 154 RTKHIELDCHVVRAKLQAGLIHLLPISTHHQLADIFTKPCP----HLCFPLLGAR 2
>BP075943
Length = 547
Score = 84.0 bits (206), Expect = 9e-17
Identities = 66/182 (36%), Positives = 97/182 (53%), Gaps = 7/182 (3%)
Frame = -3
Query: 461 SYIILALYVDDMLIAGSNMTEINRLKQQMSENFEMKDLGPAKQILGMRISRNRSEGVLKL 520
S+ L LYVDD+L+AG++M EI +K + + F +KDLG AK LG+ I+R+ S G++ L
Sbjct: 533 SFTALLLYVDDVLLAGNDMHEIQLVKSSLHDQFRIKDLGEAKYFLGLEIARSTS-GIV-L 360
Query: 521 SQEKYVEKLL-DRFNVG------DANTRSTPLGNHLKFSKKQSPQTDEEESYMSTVPYAS 573
+Q KY +L+ D + G T S LG + +P TD Y
Sbjct: 359 NQRKYALQLISDSGHFGFQPRFYSHGTNSQTLG-----TNTGTPLTD-------IGSYRR 216
Query: 574 AVGSLMYAMVCTRPDIAHAVGVVSRFMSNPGKEHWECVKWILRYLKGSSRMCLCFRRNNL 633
VG L+Y + TRPDI AV +S+F+S P H + + L+YL GS L + ++
Sbjct: 215 IVGRLLY-LNTTRPDITFAVNQLSQFLSAPTDIHEQQLTGFLKYL*GSPGSGLFYPASSS 39
Query: 634 TL 635
TL
Sbjct: 38 TL 33
>BP084332
Length = 368
Score = 80.5 bits (197), Expect = 1e-15
Identities = 39/110 (35%), Positives = 69/110 (62%), Gaps = 1/110 (0%)
Frame = +2
Query: 672 QNRVALSTTESEYVAISEAAKEMIWLKSFLKELGKEQDVPPLFSDSQSVIFLAKNPVFHS 731
Q+ +ALST E+EY++ + + +M+W+K L++ + P++ D+ + I L+KNP+ HS
Sbjct: 32 QSTIALSTAEAEYISAAICSTQMLWMKHQLEDYQILESNIPIYCDNTAAISLSKNPILHS 211
Query: 732 RCKHIQMKYHFIRELISDEELSLLKILGSENP-TDMLTKTVTADNLRLCI 780
R KHI++KYHFIR+ + L LLK + +++ D+ TK + D +
Sbjct: 212 RAKHIEVKYHFIRDYVQKGVL-LLKFVDTDHQWADIFTKPLAEDRFNFIL 358
>AV427921
Length = 284
Score = 79.7 bits (195), Expect = 2e-15
Identities = 32/87 (36%), Positives = 56/87 (63%)
Frame = +1
Query: 48 TPEQNGVAERMNRTLNERARCMRIQSGLPKMFWVDAINTAAYLINRGPSIPLDYQLPEEV 107
TP+QNGV+ER NRT+ R + SGLPK F +A+ + +++NR P++ + +PEE
Sbjct: 22 TPQQNGVSERKNRTILNMVRSLLTMSGLPKSFLPEAVMWSLHILNRSPTLVVQNMMPEEA 201
Query: 108 WSGKEVSLSHLKVFGCVSYVLIDSDRR 134
WSG++ ++ H ++ C++Y + +R
Sbjct: 202 WSGRQPAVDHFRISRCLAYAHVPDQKR 282
>AU088980
Length = 360
Score = 70.9 bits (172), Expect = 8e-13
Identities = 41/109 (37%), Positives = 54/109 (48%)
Frame = +2
Query: 52 NGVAERMNRTLNERARCMRIQSGLPKMFWVDAINTAAYLINRGPSIPLDYQLPEEVWSGK 111
N + ER +R + R + S LPK W A+ AAYLIN PS L Q P + +
Sbjct: 2 NSIVERKHRHILNVTRALMFHSYLPKNLWTFAVKHAAYLINXLPSPLLKGQCPYQFLNND 181
Query: 112 EVSLSHLKVFGCVSYVLIDSDRRDKLDPKAIKCFFIGYGFDMYGYRFWD 160
+L LKVFG + Y + KLDP+A KC F+G+ GY D
Sbjct: 182 SPTLLDLKVFGTLCYSSTLTHNXQKLDPRARKCVFLGFKTGTXGYIVMD 328
>TC19389 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-related Pol
polyprotein from transposon TNT 1-94 [Contains: Protease
; Reverse transcriptase ; Endonuclease] , partial (6%)
Length = 498
Score = 68.6 bits (166), Expect = 4e-12
Identities = 39/130 (30%), Positives = 73/130 (56%), Gaps = 1/130 (0%)
Frame = +3
Query: 657 IFTLGGTAVSWKSKLQNRVALSTTESEYVAISEAAKEMIWLKSFLKELG-KEQDVPPLFS 715
+ T G AV+W S+LQ VALST E+E++A +EA E++W+K+FL+ +
Sbjct: 3 LVTFAGGAVAWPSRLQKCVALSTAEAEFIAATEACHELLWMKNFLQNAWFHSHPILCCIV 182
Query: 716 DSQSVIFLAKNPVFHSRCKHIQMKYHFIRELISDEELSLLKILGSENPTDMLTKTVTADN 775
++++ LA+ +F + +R++++ + L L KI ++ DM+TK++ +
Sbjct: 183 ITKALFTLARILLFIQDPSTLMFVIIGLRDVLNSKLLELEKIHTDDDGADMMTKSLPREK 362
Query: 776 LRLCIASAGL 785
L +C AG+
Sbjct: 363 LEVCDMIAGM 392
>AV424544
Length = 276
Score = 61.6 bits (148), Expect = 5e-10
Identities = 32/92 (34%), Positives = 54/92 (57%)
Frame = +3
Query: 434 KFNEFMSNSGFNRCDMDHCCFVKKFADSYIILALYVDDMLIAGSNMTEINRLKQQMSENF 493
K + ++ G+ + DH F K S+ ++ +YVDD+++AG+++ EI +K ++ F
Sbjct: 6 KLSSYLHILGYIQSAHDHSLFTKFRDASFTVILVYVDDLILAGNDLNEIQCVKNKLDIQF 185
Query: 494 EMKDLGPAKQILGMRISRNRSEGVLKLSQEKY 525
+KDLG K LG+ ++ RS L LSQ KY
Sbjct: 186 RIKDLGTLKYFLGLEVA--RSSCGLFLSQRKY 275
>BP043850
Length = 515
Score = 60.5 bits (145), Expect = 1e-09
Identities = 32/81 (39%), Positives = 48/81 (58%), Gaps = 1/81 (1%)
Frame = -1
Query: 693 EMIWLKSFLKELGKEQDVP-PLFSDSQSVIFLAKNPVFHSRCKHIQMKYHFIRELISDEE 751
E+IWL+ L ELG Q P PL +D+ S I +A NPV+H +HI++ H +RE
Sbjct: 512 EIIWLRGLLSELGFLQSQPTPLHADNTSAIQIAANPVYHEWTRHIEVDCHSVREAYDRRV 333
Query: 752 LSLLKILGSENPTDMLTKTVT 772
++L + S D+LTK++T
Sbjct: 332 ITLPHVSTSVQIADILTKSLT 270
>TC16929 weakly similar to UP|Q9LFY6 (Q9LFY6) T7N9.5, partial (4%)
Length = 553
Score = 59.3 bits (142), Expect = 2e-09
Identities = 34/91 (37%), Positives = 47/91 (51%), Gaps = 1/91 (1%)
Frame = +1
Query: 696 WLKSFLKELGKEQDVPPL-FSDSQSVIFLAKNPVFHSRCKHIQMKYHFIRELISDEELSL 754
WL L++L + P L + D+ S +A NPVFH R KHI++ H +RE I + L
Sbjct: 4 WLTYLLQDLKVPFEQPALVYCDNNSARHIAANPVFHERTKHIEIDCHIVRERIQKGLIHL 183
Query: 755 LKILGSENPTDMLTKTVTADNLRLCIASAGL 785
L I SE D+ TK ++ N A GL
Sbjct: 184 LPISSSEPLADIYTKALSPQNFHQICAKLGL 276
>TC15664 weakly similar to UP|O81617 (O81617) F8M12.17 protein, partial (4%)
Length = 670
Score = 50.4 bits (119), Expect = 1e-06
Identities = 30/94 (31%), Positives = 47/94 (49%), Gaps = 1/94 (1%)
Frame = +1
Query: 693 EMIWLKSFLKELGKEQDVPPL-FSDSQSVIFLAKNPVFHSRCKHIQMKYHFIRELISDEE 751
E+ WL L++L P L + DSQS +A N VFH R KH+ + H +RE + +
Sbjct: 4 EL*WLTYILQDLRVPFISPSLLYCDSQSARHIATNAVFHERTKHLDIDCHVVREKLQAKL 183
Query: 752 LSLLKILGSENPTDMLTKTVTADNLRLCIASAGL 785
LL I + D+LTK + + ++ G+
Sbjct: 184 FHLLPISSVDQTADILTKPLESGPFSHLVSKLGV 285
>BP057233
Length = 473
Score = 46.6 bits (109), Expect = 2e-05
Identities = 22/62 (35%), Positives = 37/62 (59%)
Frame = -2
Query: 711 PPLFSDSQSVIFLAKNPVFHSRCKHIQMKYHFIRELISDEELSLLKILGSENPTDMLTKT 770
P L+ D+ S LA NPV H+R KHI++ H+IR+ + E+ + + ++ D LTK
Sbjct: 451 PILWCDNLSAKALASNPVLHARSKHIEIDVHYIRDQVLQNEVVVAYVPTTDQIADCLTKP 272
Query: 771 VT 772
++
Sbjct: 271 LS 266
>BP052632
Length = 489
Score = 43.9 bits (102), Expect = 1e-04
Identities = 21/73 (28%), Positives = 38/73 (51%)
Frame = -3
Query: 104 PEEVWSGKEVSLSHLKVFGCVSYVLIDSDRRDKLDPKAIKCFFIGYGFDMYGYRFWDEQN 163
P E+W G+ ++S+ FGC ++L +D K D K K +GY + Y ++ +
Sbjct: 481 PYELWRGRRPTVSYFHPFGCK*FILNTNDNIGKFDNKFDKRILLGYSSNSKAYIVFNSRT 302
Query: 164 KKIIRSRNVTFNE 176
+ + S NV F++
Sbjct: 301 QVVEESINVKFDD 263
>BP084259
Length = 385
Score = 43.9 bits (102), Expect = 1e-04
Identities = 22/57 (38%), Positives = 32/57 (55%), Gaps = 1/57 (1%)
Frame = -3
Query: 687 ISEAAKEMIWLKSFLKELGKE-QDVPPLFSDSQSVIFLAKNPVFHSRCKHIQMKYHF 742
I A E +WL+ LKEL VP +FS++ + ++ N VFHS KH+ + HF
Sbjct: 173 IGSTAAEFLWLQQLLKELQFSLPKVPCIFSNNINATYVCLNLVFHSHMKHVALDCHF 3
>TC10186 weakly similar to UP|Q9FH39 (Q9FH39) Copia-type polyprotein,
partial (4%)
Length = 528
Score = 32.3 bits (72), Expect(2) = 7e-04
Identities = 14/22 (63%), Positives = 17/22 (76%)
Frame = +2
Query: 716 DSQSVIFLAKNPVFHSRCKHIQ 737
D++S I LAKNPV H R KHI+
Sbjct: 5 DNKSAIDLAKNPVSHGRSKHIE 70
Score = 27.7 bits (60), Expect(2) = 7e-04
Identities = 12/43 (27%), Positives = 24/43 (54%)
Frame = +3
Query: 735 HIQMKYHFIRELISDEELSLLKILGSENPTDMLTKTVTADNLR 777
+I+ KYHF+R+ ++ ++SL D++TK + + R
Sbjct: 69 NIETKYHFLRDQVTKGKISLKHCGTDLQVADIMTKGLKTERFR 197
>TC18909
Length = 621
Score = 30.4 bits (67), Expect(2) = 0.015
Identities = 12/21 (57%), Positives = 17/21 (80%)
Frame = +2
Query: 351 DFTEIFSPVVKMTTIRVILSI 371
D+TE SPVVK T+R++LS+
Sbjct: 62 DYTETVSPVVKPVTVRILLSL 124
Score = 25.0 bits (53), Expect(2) = 0.015
Identities = 10/13 (76%), Positives = 11/13 (83%)
Frame = +1
Query: 334 RYKARLVVKGFQQ 346
+YKARLV KGF Q
Sbjct: 13 KYKARLVAKGFHQ 51
>BP037734
Length = 490
Score = 31.2 bits (69), Expect(2) = 0.016
Identities = 18/66 (27%), Positives = 28/66 (42%), Gaps = 11/66 (16%)
Frame = +2
Query: 391 GDLEEEIYMTQPEGF--EVLGTKNLVCKLHKS---------LYGLKQAPRQWYKKFNEFM 439
G ++ E+ + F E+ +C H LYGLKQ PR W+++F
Sbjct: 71 GKIKSELIIISLRNFNRELPNRMTWICSYHSMTIIDHCW**LYGLKQLPRDWFERFTFLR 250
Query: 440 SNSGFN 445
S + N
Sbjct: 251 SKAVHN 268
Score = 24.3 bits (51), Expect(2) = 0.016
Identities = 12/31 (38%), Positives = 21/31 (67%)
Frame = +3
Query: 483 NRLKQQMSENFEMKDLGPAKQILGMRISRNR 513
+RLK ++ F+++DLGP L + I+R+R
Sbjct: 369 DRLKNSLTAEFKIEDLGPLTCFLEI-IARSR 458
>TC17272
Length = 437
Score = 33.9 bits (76), Expect = 0.10
Identities = 25/77 (32%), Positives = 40/77 (51%), Gaps = 1/77 (1%)
Frame = +3
Query: 630 RNNLTLQEFSDADLGGDSDGGKSTTGYIFTLGGTAVSWKSKLQNRVALSTTESEYVAISE 689
R++L L++ +D D+ + DG KS +G G + S KL + ++ ++E
Sbjct: 231 RDDLVLEDCADLDIDREPDGRKSKSG-----GALSCSQDCKLTIN*S*ASADAE------ 377
Query: 690 AAKEMIWLKS-FLKELG 705
A KEM+WLK F K G
Sbjct: 378 AGKEMLWLKQVFFKS*G 428
>TC14791 similar to UP|Q9LJ04 (Q9LJ04) ESTs AU082210(C53655), partial (37%)
Length = 1779
Score = 33.5 bits (75), Expect = 0.14
Identities = 17/61 (27%), Positives = 32/61 (51%)
Frame = +2
Query: 198 SERVALEEISESDVVKRNQINPENEDDEVEVELEQEPTTVIETPITQVRRSTRTPKAPQR 257
+E A + S+ V N E E++E ++ELE+EP + P T+ + + +A ++
Sbjct: 374 NESSAAAAAAASESVANGSNNNEEEEEEEDLELEEEPVERLLEPFTREQLHSLVKQAAEK 553
Query: 258 Y 258
Y
Sbjct: 554 Y 556
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.318 0.134 0.392
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,660,375
Number of Sequences: 28460
Number of extensions: 164234
Number of successful extensions: 829
Number of sequences better than 10.0: 52
Number of HSP's better than 10.0 without gapping: 823
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 824
length of query: 799
length of database: 4,897,600
effective HSP length: 98
effective length of query: 701
effective length of database: 2,108,520
effective search space: 1478072520
effective search space used: 1478072520
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 59 (27.3 bits)
Lotus: description of TM0065.18