BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0065.12
(1309 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC12832 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-rela... 159 3e-39
BP066094 91 2e-18
TC8301 74 1e-13
AV424544 63 3e-10
TC8302 63 3e-10
BP075943 62 4e-10
BP063694 54 2e-08
TC9039 55 9e-08
BP033602 50 2e-06
AV766088 48 1e-05
BP063902 43 4e-04
AV421607 36 0.035
BP081717 35 0.10
TC17853 similar to UP|Q42412 (Q42412) RNA-binding protein RZ-1, ... 34 0.13
TC9521 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, part... 34 0.13
BP058111 34 0.13
TC19389 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-rela... 29 0.14
TC9337 similar to UP|PME_PRUPE (Q43062) Pectinesterase PPE8B pre... 34 0.17
BP065763 33 0.22
TC11095 similar to PIR|T05112|T05112 splicing factor 9G8-like SR... 32 0.50
>TC12832 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-related Pol
polyprotein from transposon TNT 1-94 [Contains: Protease
; Reverse transcriptase ; Endonuclease] , partial (9%)
Length = 747
Score = 159 bits (402), Expect = 3e-39
Identities = 76/143 (53%), Positives = 101/143 (70%)
Frame = +2
Query: 954 FDSYMLRIGYRRCDYDCCVYVRSLDDGSFIFLLLYVDDMLIAANHLHNVNELKTKLGKEF 1013
FDS+++ +GY R D C Y + DD FI LLLYVDDML+ + V ELK +L +EF
Sbjct: 2 FDSFIMSLGYNRLSSDHCTYHKRFDDNDFIILLLYVDDMLVVGPNKDRVQELKAQLAREF 181
Query: 1014 DMKDLGAAKKILGMEIHRDRGTKKLWLSQKSYVEGVLSRFDMSKENPVSTPLANHFKLSL 1073
DMKDLG A KILGM+IHRDR +++WLSQK+Y++ VL RF+M NP+STPL ++KLS
Sbjct: 182 DMKDLGPANKILGMQIHRDRKDRRIWLSQKNYLQKVLRRFNMQDCNPISTPLPVNYKLSS 361
Query: 1074 GQCSKTDSEIEGMSKIPYASAVG 1096
+++E MS++PYASAVG
Sbjct: 362 SMIPSSEAERMEMSRVPYASAVG 430
>BP066094
Length = 532
Score = 90.5 bits (223), Expect = 2e-18
Identities = 46/146 (31%), Positives = 85/146 (57%)
Frame = +2
Query: 883 SIRAVLALVASRDMHLEQMDVKTTFLHGNLEEQIYMEQPEGFSETGDGRLVCKLKRSLYG 942
+IR +++ + ++ L QM+VK+ FL+G + E++Y+ QP G + + + KLK+SLYG
Sbjct: 98 AIRLLISFSVNHNIILHQMNVKSAFLNGYISEEVYVHQPPGXEDEKNSDHIFKLKKSLYG 277
Query: 943 LKQSPRQ*YKRFDSYMLRIGYRRCDYDCCVYVRSLDDGSFIFLLLYVDDMLIAANHLHNV 1002
LKQ+PR Y+R S++L R D ++ ++ D + + +YVDD++ + +
Sbjct: 278 LKQAPRAWYERLSSFLLENEXVRGKVDTTLFCKTYKD-DILIVQIYVDDIIFGSANPSLC 454
Query: 1003 NELKTKLGKEFDMKDLGAAKKILGME 1028
E + EF+M+ +G K LG++
Sbjct: 455 KEFSEMMQAEFEMRMMGELKYFLGIQ 532
>TC8301
Length = 1243
Score = 74.3 bits (181), Expect = 1e-13
Identities = 40/127 (31%), Positives = 71/127 (55%)
Frame = +2
Query: 2 AKFEVTRFYGTGNFGLWHRRVKDLLAQQSLQKALRDEKPTDIATVDWNEMKEKAASLIRL 61
AKFE+ +F G+ NF LW ++K +L + + A+ D +P DI W EM + A + + L
Sbjct: 506 AKFEIPKFSGS-NFSLWKLKIKAILRKDNCLPAI-DGRPADITDDKWKEMDDNAVANLHL 679
Query: 62 CVSDDLMNHILDLTTPKDVWDKLESLYMSKTLMNKLFAKQRLYSLKMQEGGDLQAHVYAF 121
V+D +++ I + T K++WD L LY K+L N++ ++ L S D +H +
Sbjct: 680 AVADSVLSSIAEKKTAKEIWDTLIQLYEVKSLHNRILKEETLNSSNE*IHFDAGSHQQSE 859
Query: 122 NNILADL 128
+N+ + +
Sbjct: 860 HNVCSTI 880
>AV424544
Length = 276
Score = 63.2 bits (152), Expect = 3e-10
Identities = 34/93 (36%), Positives = 57/93 (60%)
Frame = +3
Query: 953 RFDSYMLRIGYRRCDYDCCVYVRSLDDGSFIFLLLYVDDMLIAANHLHNVNELKTKLGKE 1012
+ SY+ +GY + +D ++ + D SF +L+YVDD+++A N L+ + +K KL +
Sbjct: 6 KLSSYLHILGYIQSAHDHSLFTK-FRDASFTVILVYVDDLILAGNDLNEIQCVKNKLDIQ 182
Query: 1013 FDMKDLGAAKKILGMEIHRDRGTKKLWLSQKSY 1045
F +KDLG K LG+E+ R + L+LSQ+ Y
Sbjct: 183 FRIKDLGTLKYFLGLEV--ARSSCGLFLSQRKY 275
>TC8302
Length = 494
Score = 62.8 bits (151), Expect = 3e-10
Identities = 42/145 (28%), Positives = 68/145 (45%), Gaps = 2/145 (1%)
Frame = +3
Query: 101 QRLYSLKMQEGGDLQAHVYAFNNILADLTRLRVKVDDEDKAIILLRSLPGSYDHLVTTLT 160
+RLY+L+M E + H+ N + A L+ + + ++A +LL SLP SYD LV +
Sbjct: 3 KRLYTLRMSESTSMSDHIDHMNTLFAQLSASNFTIGENERAELLLESLPDSYDQLVINIK 182
Query: 161 YGK--DSIKLDSISSTLLQHAQRRQSVEEGGGSSGKCLFVKGGQGRARGKGKAVDSGKKK 218
+ + L +S LL+ +R + E+ SS K + +
Sbjct: 183 NNNIVNHLPLMMLSEVLLEEDSQR*NKEDR*DSS---------------KPMEALTMTRG 317
Query: 219 RSKSKDRKTAECYSCKQIGHWKRDC 243
RSKS+ + +CY C Q H K+DC
Sbjct: 318 RSKSRKKINLKCYHCGQR*HLKKDC 392
>BP075943
Length = 547
Score = 62.4 bits (150), Expect = 4e-10
Identities = 33/72 (45%), Positives = 47/72 (64%)
Frame = -3
Query: 980 GSFIFLLLYVDDMLIAANHLHNVNELKTKLGKEFDMKDLGAAKKILGMEIHRDRGTKKLW 1039
GSF LLLYVDD+L+A N +H + +K+ L +F +KDLG AK LG+EI R T +
Sbjct: 536 GSFTALLLYVDDVLLAGNDMHEIQLVKSSLHDQFRIKDLGEAKYFLGLEI--ARSTSGIV 363
Query: 1040 LSQKSYVEGVLS 1051
L+Q+ Y ++S
Sbjct: 362 LNQRKYALQLIS 327
>BP063694
Length = 511
Score = 53.9 bits (128), Expect(2) = 2e-08
Identities = 27/83 (32%), Positives = 42/83 (50%), Gaps = 1/83 (1%)
Frame = -2
Query: 416 KMWHMRLGHLSERGMMQLHKRNLLKGVRSCTIGLC-KYCVLGKQCRVRFKTGQHKTKGIL 474
++WH RLGH++ + QLHK L +C C + K R+ F + +L
Sbjct: 363 ELWHSRLGHVNFDIIKQLHKHGCLDVSSILPKPICCTSCQMAKSKRLVFHDNNKRASAVL 184
Query: 475 DYVHSDVWGPTKEPSVGGFRYFL 497
D +H D+ GP+ S+ GF YF+
Sbjct: 183 DLIHCDLRGPSPVASIDGFSYFV 115
Score = 22.3 bits (46), Expect(2) = 2e-08
Identities = 12/25 (48%), Positives = 16/25 (64%)
Frame = -3
Query: 492 GFRYFLHLLMIFLVRSGFIF*NINL 516
GF L+LLM V GFI *+++L
Sbjct: 131 GFLTLLYLLMTSPVLLGFIH*SVSL 57
>TC9039
Length = 1218
Score = 54.7 bits (130), Expect = 9e-08
Identities = 45/215 (20%), Positives = 89/215 (40%), Gaps = 2/215 (0%)
Frame = +2
Query: 31 LQKALRDEKPTDIATVDWNEMKEKAASLIRLCVSDDLMNHILD-LTTPKDVWDKLESLYM 89
L+K+ E+ D W+ + +I+ + + I + + KD ++E +
Sbjct: 260 LRKSSTSEQRKDYEK--WDRSNRMSLMIIKRGIPEVFRGTISEEIKGAKDFLAEIEKRFA 433
Query: 90 SKTLMNKLFAKQRLYSLKMQEGGDLQAHVYAFNNILADLTRLRVKVDDEDKAIILLRSLP 149
Q L S+K Q G+++ ++ +NI + L L++++ D+ ++L SLP
Sbjct: 434 KSDKAETSTLLQNLISMKYQGKGNIREYIMGMSNIASKLKALKLELSDDLLIHLVLLSLP 613
Query: 150 GSYDHLVTTLTYGKDSIKLDSISSTLLQHAQRRQSVEEGGGSSGKCLFVKGGQGRA-RGK 208
+ + K+ L+ + S +Q +R + + KG + + K
Sbjct: 614 AQFSQFKISYNCPKEKWSLNELISFCVQEEERLKQERKESAHFVSTSKDKGKRKKTVEPK 793
Query: 209 GKAVDSGKKKRSKSKDRKTAECYSCKQIGHWKRDC 243
+A D+ K+ K D CY C GH K+ C
Sbjct: 794 NEAADAPAPKKQKEDDT----CYFCNVSGHMKKKC 886
Score = 36.6 bits (83), Expect = 0.026
Identities = 17/55 (30%), Positives = 29/55 (51%)
Frame = +3
Query: 310 YVYLGDDKPCIIKGMRQVKIALDDGRVRTLSQVRYVPEVTKNMISLGTLHENGYS 364
Y+Y+GD K ++ + ++ L G L VP +N+IS+ L ++GYS
Sbjct: 957 YIYVGDGKTVEVEAIGHFRLLLCTGFYLDLKDTFVVPSFRRNLISVSNLDKSGYS 1121
>BP033602
Length = 533
Score = 50.1 bits (118), Expect = 2e-06
Identities = 29/85 (34%), Positives = 45/85 (52%)
Frame = +3
Query: 413 DATKMWHMRLGHLSERGMMQLHKRNLLKGVRSCTIGLCKYCVLGKQCRVRFKTGQHKTKG 472
D +WH RLGH + + + L +L K V +C+ C+ CVL K R + + +
Sbjct: 264 DQIMLWHNRLGHPNFQYLRHLFP-DLFKNV-NCSSLECESCVLAKNQRAPYYSQPYHASR 437
Query: 473 ILDYVHSDVWGPTKEPSVGGFRYFL 497
+HSDVWGP+K + G R+F+
Sbjct: 438 PFYLIHSDVWGPSKITTQFGKRWFV 512
>AV766088
Length = 501
Score = 47.8 bits (112), Expect = 1e-05
Identities = 27/86 (31%), Positives = 37/86 (42%)
Frame = -3
Query: 414 ATKMWHMRLGHLSERGMMQLHKRNLLKGVRSCTIGLCKYCVLGKQCRVRFKTGQHKTKGI 473
A +WH RLGH S + L + +C+ CV GK R+ F + + T
Sbjct: 316 AQSLWHSRLGHPSSSALRYLRSNKFISYELLNYSPVCESCVFGKHVRLPFVSSNNVTVMP 137
Query: 474 LDYVHSDVWGPTKEPSVGGFRYFLHL 499
D +HSD+W S G Y L L
Sbjct: 136 FDILHSDLWTSPVLSSAGHRFYVLFL 59
>BP063902
Length = 494
Score = 42.7 bits (99), Expect = 4e-04
Identities = 28/95 (29%), Positives = 44/95 (45%)
Frame = -3
Query: 371 RDILRVSKGAMTVMRAKRTAGNIYKLLGGTVMGDVASVETDDDATKMWHMRLGHLSERGM 430
R ILR K M ++R + G++Y + + +AS +WH RLGH + +
Sbjct: 294 RPILRHLKTGMPLLRCN-SLGDLYPVTRSSPFAGLAS--------SVWHHRLGHPASSAL 142
Query: 431 MQLHKRNLLKGVRSCTIGLCKYCVLGKQCRVRFKT 465
L L+ S + +C CVLGK R+ F +
Sbjct: 141 NHLRNNKLIFCEPSRSSSVCDSCVLGKHVRLPFSS 37
>AV421607
Length = 245
Score = 36.2 bits (82), Expect = 0.035
Identities = 19/58 (32%), Positives = 24/58 (40%)
Frame = +3
Query: 201 GQGRARGKGKAVDSGKKKRSKSKDRKTAECYSCKQIGHWKRDCPNRPGKSSNSNSSAN 258
G G G G +G CY C + GHW RDCP+ + NSN + N
Sbjct: 9 GSGPGSGSGSGTATG--------------CYKCGRPGHWSRDCPS---SAPNSNPNPN 131
>BP081717
Length = 291
Score = 34.7 bits (78), Expect = 0.10
Identities = 17/55 (30%), Positives = 29/55 (51%)
Frame = -1
Query: 417 MWHMRLGHLSERGMMQLHKRNLLKGVRSCTIGLCKYCVLGKQCRVRFKTGQHKTK 471
MWH RLGH S + + + K + V+ + +C +C + KQ ++ F K+K
Sbjct: 168 MWHFRLGHPSSKVLQHISK--VFPYVKLHSNIICDFCHMAKQTKLPFPISDTKSK 10
>TC17853 similar to UP|Q42412 (Q42412) RNA-binding protein RZ-1, partial
(58%)
Length = 881
Score = 34.3 bits (77), Expect = 0.13
Identities = 21/65 (32%), Positives = 32/65 (48%), Gaps = 14/65 (21%)
Frame = +2
Query: 200 GGQGRARGKGKAVDSGKKKRSK----SKDRKTAECYSCKQIGHWKRDCPNR--------- 246
G + R RG+ + D G + RS+ S+ EC+ C + GH+ R+CP+
Sbjct: 320 GDRYRERGRDRD-DRGDRDRSRGYGGSRGSNGGECFKCGKPGHFARECPSEGETRRGKVW 496
Query: 247 -PGKS 250
PGKS
Sbjct: 497 WPGKS 511
>TC9521 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, partial (56%)
Length = 598
Score = 34.3 bits (77), Expect = 0.13
Identities = 24/74 (32%), Positives = 36/74 (48%), Gaps = 4/74 (5%)
Frame = +1
Query: 182 RQSVEEGGGSSGKCLFVKGGQGRARGKGKA----VDSGKKKRSKSKDRKTAECYSCKQIG 237
R S E GGG ++ G+G G G+ +D + K+ D K +CY C + G
Sbjct: 310 RGSREGGGGRDRDREYM--GRGPPPGSGRCFNCGIDGHWARDCKAGDWKN-KCYRCGERG 480
Query: 238 HWKRDCPNRPGKSS 251
H +++C N P K S
Sbjct: 481 HIEKNCKNSPKKLS 522
Score = 32.3 bits (72), Expect = 0.50
Identities = 17/60 (28%), Positives = 26/60 (43%), Gaps = 3/60 (5%)
Frame = +1
Query: 187 EGGGSSGKCLFVKGGQGRARGK---GKAVDSGKKKRSKSKDRKTAECYSCKQIGHWKRDC 243
+G G L V+ +G RG G D ++ + + C++C GHW RDC
Sbjct: 253 DGRDVDGSRLIVEFAKGVPRGSREGGGGRDRDREYMGRGPPPGSGRCFNCGIDGHWARDC 432
>BP058111
Length = 570
Score = 34.3 bits (77), Expect = 0.13
Identities = 18/63 (28%), Positives = 29/63 (45%)
Frame = -1
Query: 417 MWHMRLGHLSERGMMQLHKRNLLKGVRSCTIGLCKYCVLGKQCRVRFKTGQHKTKGILDY 476
+WH+RLGH S + + L K + T C C + KQ ++ F + I D
Sbjct: 186 LWHLRLGHTSSKKLAILQKNFPFITCKKIT-SPCDTCHMAKQKKLSFPNSVTLSSEIFDL 10
Query: 477 VHS 479
+H+
Sbjct: 9 IHT 1
>TC19389 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-related Pol
polyprotein from transposon TNT 1-94 [Contains: Protease
; Reverse transcriptase ; Endonuclease] , partial (6%)
Length = 498
Score = 28.9 bits (63), Expect(2) = 0.14
Identities = 18/39 (46%), Positives = 21/39 (53%)
Frame = +1
Query: 1181 LLLQGDLYVGSHQFNP**LCLQLRQNTWQ*LKLPRKHCG 1219
LLLQG+L+ G H L L LRQ+ LKL CG
Sbjct: 7 LLLQGELWHGHHDCRSVLLYLLLRQSLLLQLKLAMSCCG 123
Score = 23.9 bits (50), Expect(2) = 0.14
Identities = 12/35 (34%), Positives = 17/35 (48%)
Frame = +3
Query: 1226 SWVLSEVEFSCIVIVRVLSIWRKIRCIMLEPSILM 1260
+W S CIVI + L +I + +PS LM
Sbjct: 144 AWFHSHPILCCIVITKALFTLARILLFIQDPSTLM 248
>TC9337 similar to UP|PME_PRUPE (Q43062) Pectinesterase PPE8B precursor
(Pectin methylesterase) (PE) , partial (54%)
Length = 1145
Score = 33.9 bits (76), Expect = 0.17
Identities = 14/34 (41%), Positives = 20/34 (58%)
Frame = +3
Query: 449 LCKYCVLGKQCRVRFKTGQHKTKGILDYVHSDVW 482
+C CVLGK R+ F + + T D +HSD+W
Sbjct: 1026 VCDSCVLGKHVRLPFSSSETITLRPFDILHSDLW 1127
>BP065763
Length = 537
Score = 33.5 bits (75), Expect = 0.22
Identities = 15/40 (37%), Positives = 22/40 (54%), Gaps = 8/40 (20%)
Frame = -1
Query: 215 GKKKRSKSKDRKTAE--------CYSCKQIGHWKRDCPNR 246
G+K++ K + K E CY CK+ GH+K+ CP R
Sbjct: 450 GRKEQKKDQALKVKEGRIHKEHVCYFCKKAGHFKKYCPKR 331
>TC11095 similar to PIR|T05112|T05112 splicing factor 9G8-like SR protein
RSZp22 [validated] - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (89%)
Length = 912
Score = 32.3 bits (72), Expect = 0.50
Identities = 18/57 (31%), Positives = 24/57 (41%)
Frame = +1
Query: 200 GGQGRARGKGKAVDSGKKKRSKSKDRKTAECYSCKQIGHWKRDCPNRPGKSSNSNSS 256
GG G RG G++ G K CY C + GH+ R+C R G + S
Sbjct: 313 GGGGGGRGGGRSGGGGGGSDMK--------CYECGEPGHFARECRMRGGSGRRRSRS 459
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.337 0.146 0.474
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 24,074,650
Number of Sequences: 28460
Number of extensions: 360282
Number of successful extensions: 2764
Number of sequences better than 10.0: 63
Number of HSP's better than 10.0 without gapping: 2692
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2733
length of query: 1309
length of database: 4,897,600
effective HSP length: 101
effective length of query: 1208
effective length of database: 2,023,140
effective search space: 2443953120
effective search space used: 2443953120
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.7 bits)
S2: 61 (28.1 bits)
Lotus: description of TM0065.12