BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0064b.12
(223 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BI418455 307 1e-84
TC9089 similar to UP|RHRE_PEA (Q9S8P4) Rhicadhesin receptor prec... 195 6e-51
BP079498 162 3e-41
TC16696 similar to UP|Q9AR81 (Q9AR81) Germin-like protein precur... 130 2e-31
TC11836 similar to GB|AAB51577.1|1755176|ATU75199 germin-like pr... 129 3e-31
TC8987 similar to UP|ABPA_PRUPE (Q9ZRA4) Auxin-binding protein A... 128 9e-31
TC11913 weakly similar to UP|ABPA_PRUPE (Q9ZRA4) Auxin-binding p... 90 3e-19
TC9088 similar to UP|RHRE_PEA (Q9S8P4) Rhicadhesin receptor prec... 66 4e-12
AV423569 35 0.010
TC13251 similar to UP|Q9AVK8 (Q9AVK8) Allergen Gly m Bd 28K (Fra... 28 1.3
CB828647 28 1.7
TC8469 similar to UP|Q9MAH9 (Q9MAH9) F12M16.12, partial (48%) 25 8.2
TC15046 similar to UP|Q9FVN7 (Q9FVN7) NADPH-dependent mannose 6-... 25 8.2
>BI418455
Length = 477
Score = 307 bits (786), Expect = 1e-84
Identities = 151/154 (98%), Positives = 153/154 (99%)
Frame = +1
Query: 1 MKVVYFLVTILALASSFASAYDPSPLQDFCVGINDPKDGVFVNGKFCKDPALVKAEDFFK 60
M+VVYFLVTILALASSFASAYDPSPLQDFCVGINDPK GVFVNGKFCKDPALVKAEDFFK
Sbjct: 16 MEVVYFLVTILALASSFASAYDPSPLQDFCVGINDPKYGVFVNGKFCKDPALVKAEDFFK 195
Query: 61 HVEPGSTSNPLGSKVTPVTVEQLFGLNTLGISLARIDFAPKGLNPPHTHPRATEILIVLE 120
HVEPG+TSNPLGSKVTPVTVEQLFGLNTLGISLARIDFAPKGLNPPHTHPRATEILIVLE
Sbjct: 196 HVEPGNTSNPLGSKVTPVTVEQLFGLNTLGISLARIDFAPKGLNPPHTHPRATEILIVLE 375
Query: 121 GTLYVGFVTSNNANGGNRLFTKVLNKGDVFVFPI 154
GTLYVGFVTSNNANGGNRLFTKVLNKGDVFVFPI
Sbjct: 376 GTLYVGFVTSNNANGGNRLFTKVLNKGDVFVFPI 477
>TC9089 similar to UP|RHRE_PEA (Q9S8P4) Rhicadhesin receptor precursor
(Germin-like protein), partial (93%)
Length = 981
Score = 195 bits (495), Expect = 6e-51
Identities = 106/214 (49%), Positives = 141/214 (65%), Gaps = 2/214 (0%)
Frame = +1
Query: 3 VVYFLVTILALASSFASAYDPSPLQDFCVGINDPKDGVFVNGKFCKDPALVKAEDFFKHV 62
+++ L L L ++F+S DP LQD CV DP GV VNG CK+ + V A DFF ++
Sbjct: 175 LLFALPLALLLTTAFSS--DPDYLQDLCVA--DPASGVPVNGFTCKEASKVNASDFFSNI 342
Query: 63 --EPGSTSNPLGSKVTPVTVEQLFGLNTLGISLARIDFAPKGLNPPHTHPRATEILIVLE 120
+PG+T+N GS VT V+++ GLNTLG+SLARID+AP GLNPPHTHPRATE++ VLE
Sbjct: 343 LAKPGATNNTFGSLVTGANVQKVPGLNTLGVSLARIDYAPDGLNPPHTHPRATEVVFVLE 522
Query: 121 GTLYVGFVTSNNANGGNRLFTKVLNKGDVFVFPIGLIHFQLNVGSGNAVAIAGLSSQNPG 180
G L VGF+T+ GN L +K + KGD+FVFP GL+HFQ N G A ++ +SQ PG
Sbjct: 523 GELDVGFITT-----GNVLISKHIVKGDIFVFPKGLLHFQKNXGKVPAAVLSAFNSQLPG 687
Query: 181 VITIANALFKASPPISQEVLTKALQVDKSIIDNL 214
+IA LF ++P + VLTK QV I+ +
Sbjct: 688 TQSIATTLFASTPTVPDNVLTKTFQVGTKQIEKI 789
>BP079498
Length = 373
Score = 162 bits (411), Expect = 3e-41
Identities = 82/83 (98%), Positives = 82/83 (98%)
Frame = -2
Query: 141 TKVLNKGDVFVFPIGLIHFQLNVGSGNAVAIAGLSSQNPGVITIANALFKASPPISQEVL 200
TKVLNKGDVFVFPI LIHFQLNVGSGNAVAIAGLSSQNPGVITIANALFKASPPISQEVL
Sbjct: 372 TKVLNKGDVFVFPISLIHFQLNVGSGNAVAIAGLSSQNPGVITIANALFKASPPISQEVL 193
Query: 201 TKALQVDKSIIDNLLKKSWYDNN 223
TKALQVDKSIIDNLLKKSWYDNN
Sbjct: 192 TKALQVDKSIIDNLLKKSWYDNN 124
>TC16696 similar to UP|Q9AR81 (Q9AR81) Germin-like protein precursor,
partial (96%)
Length = 834
Score = 130 bits (326), Expect = 2e-31
Identities = 85/213 (39%), Positives = 120/213 (55%), Gaps = 8/213 (3%)
Frame = +1
Query: 4 VYFLVTILALASSFASAYDPSPLQDFCVGINDPKDGVFVNGKF---CKDPALVKAEDFFK 60
+ F++++L+L+ + + DFCVG D F NG CK P+ V A+DF
Sbjct: 7 ILFILSLLSLSHA--------SVVDFCVG-----DLTFPNGPAGYACKKPSKVTADDFAY 147
Query: 61 HVE--PGSTSNPLGSKVTPVTVEQLFGLNTLGISLARIDFAPKGLNPPHTHPRATEILIV 118
G+TS + + VTP Q G+N LGISLAR+D A G+ P HTHP A+E L+V
Sbjct: 148 SGLGIAGNTSYIIKAAVTPAFDAQFPGVNGLGISLARLDLASGGVIPLHTHPGASEALVV 327
Query: 119 LEGTLYVGFVTSNNANGGNRLFTKVLNKGDVFVFPIGLIHFQLNVGSGNAVAIAGLSSQN 178
++GT+ GFV S+N ++ K L KGDV VFP GL+HFQ++ G +A+A SS N
Sbjct: 328 VQGTICAGFVASDNT-----VYLKTLKKGDVMVFPQGLVHFQIHDGGSSALAFVSFSSAN 492
Query: 179 PGVITIANALFKASPP---ISQEVLTKALQVDK 208
PG+ + ALFK+ P I+ A Q+ K
Sbjct: 493 PGLQILDFALFKSDFPSDLIAATAFLDAAQIKK 591
>TC11836 similar to GB|AAB51577.1|1755176|ATU75199 germin-like protein
{Arabidopsis thaliana;} , partial (56%)
Length = 434
Score = 129 bits (325), Expect = 3e-31
Identities = 65/132 (49%), Positives = 89/132 (67%), Gaps = 2/132 (1%)
Frame = +3
Query: 1 MKVVYFLVTILALASSFASAYDPSPLQDFCVGINDPKDGVFVNGKFCKDPALVKAEDFFK 60
+ ++ +V +L ++F +A DP LQD CV D + VNG CK + VK DFF
Sbjct: 45 LHIMKLVVLLLLTLTTFTAASDPDSLQDICVA--DLSSAIKVNGFVCKSASAVKETDFFS 218
Query: 61 HV--EPGSTSNPLGSKVTPVTVEQLFGLNTLGISLARIDFAPKGLNPPHTHPRATEILIV 118
+ +PG+T+ GS VT VE++ GLNTLG+S++RID+AP GLNPPHTHPRATE++ V
Sbjct: 219 AILAKPGATNTTFGSVVTGANVEKIPGLNTLGVSISRIDYAPGGLNPPHTHPRATEVVFV 398
Query: 119 LEGTLYVGFVTS 130
LEG L VGF+T+
Sbjct: 399 LEGQLDVGFITT 434
>TC8987 similar to UP|ABPA_PRUPE (Q9ZRA4) Auxin-binding protein ABP19a
precursor, partial (97%)
Length = 985
Score = 128 bits (321), Expect = 9e-31
Identities = 82/213 (38%), Positives = 124/213 (57%), Gaps = 2/213 (0%)
Frame = +1
Query: 4 VYFLVTILALASSFASAYDPSPLQDFCVGINDPKDGVFVNGKFCKDPALVKAEDF-FKHV 62
+ FL ++L L++S A+ +QDFCV DG G CK PA V ++DF F +
Sbjct: 37 IVFLFSLL-LSTSHAA------VQDFCVADLKGSDGPA--GYPCKTPATVTSDDFVFAGL 189
Query: 63 E-PGSTSNPLGSKVTPVTVEQLFGLNTLGISLARIDFAPKGLNPPHTHPRATEILIVLEG 121
PG+ +N +G+ VTP V Q G+N LG+S AR+D P G+ P HTHP A E+LIV +G
Sbjct: 190 SAPGNITNIIGAAVTPAFVGQFPGVNGLGLSAARLDIGPGGVIPLHTHPGANELLIVTQG 369
Query: 122 TLYVGFVTSNNANGGNRLFTKVLNKGDVFVFPIGLIHFQLNVGSGNAVAIAGLSSQNPGV 181
+ GF++S + ++ K L KG++ VFP GL+HFQ+ G A+ A SS +PG+
Sbjct: 370 HITAGFISS-----ASTVYIKTLKKGELMVFPQGLLHFQVAAGKTKAIGFAIFSSASPGL 534
Query: 182 ITIANALFKASPPISQEVLTKALQVDKSIIDNL 214
+ ALF ++ S ++TK +D +++ L
Sbjct: 535 QILDFALFASN--FSTPLITKTTFLDPALVKKL 627
>TC11913 weakly similar to UP|ABPA_PRUPE (Q9ZRA4) Auxin-binding protein
ABP19a precursor, partial (61%)
Length = 530
Score = 90.1 bits (222), Expect = 3e-19
Identities = 45/104 (43%), Positives = 66/104 (63%)
Frame = +1
Query: 86 LNTLGISLARIDFAPKGLNPPHTHPRATEILIVLEGTLYVGFVTSNNANGGNRLFTKVLN 145
+N LG+S AR+D G+ P H+HP A+E++I+L+G + VGF++++N ++ K L
Sbjct: 16 INGLGLSAARLDLDAGGVVPIHSHPGASELVIILQGRITVGFISTDNT-----VYQKTLM 180
Query: 146 KGDVFVFPIGLIHFQLNVGSGNAVAIAGLSSQNPGVITIANALF 189
KGD+ V P GL+HFQLN G A A+ SS NP + ALF
Sbjct: 181 KGDIIVIPQGLLHFQLNAGGNRASAVLTFSSTNPVAQLVDVALF 312
>TC9088 similar to UP|RHRE_PEA (Q9S8P4) Rhicadhesin receptor precursor
(Germin-like protein), partial (35%)
Length = 475
Score = 66.2 bits (160), Expect = 4e-12
Identities = 31/69 (44%), Positives = 43/69 (61%)
Frame = +3
Query: 146 KGDVFVFPIGLIHFQLNVGSGNAVAIAGLSSQNPGVITIANALFKASPPISQEVLTKALQ 205
KG++FVFP GL+H+Q N G A I+ +SQ PG ++I ALF A PP+ VLTKA Q
Sbjct: 3 KGEIFVFPKGLVHYQKNNGYWPASVISAFNSQLPGTVSIPLALFAAKPPVPDNVLTKAFQ 182
Query: 206 VDKSIIDNL 214
++ +
Sbjct: 183 TSTEEVEKI 209
>AV423569
Length = 351
Score = 35.0 bits (79), Expect = 0.010
Identities = 13/16 (81%), Positives = 15/16 (93%)
Frame = +3
Query: 94 ARIDFAPKGLNPPHTH 109
+RID+AP GLNPPHTH
Sbjct: 303 SRIDYAPGGLNPPHTH 350
>TC13251 similar to UP|Q9AVK8 (Q9AVK8) Allergen Gly m Bd 28K (Fragment),
partial (11%)
Length = 484
Score = 28.1 bits (61), Expect = 1.3
Identities = 12/36 (33%), Positives = 20/36 (55%)
Frame = -1
Query: 136 GNRLFTKVLNKGDVFVFPIGLIHFQLNVGSGNAVAI 171
G+ L + L GD++V P G + +N+G G + I
Sbjct: 382 GDELSERRLKTGDLYVIPAGSAFYLVNLGEGQRLHI 275
>CB828647
Length = 527
Score = 27.7 bits (60), Expect = 1.7
Identities = 11/24 (45%), Positives = 15/24 (61%)
Frame = -2
Query: 127 FVTSNNANGGNRLFTKVLNKGDVF 150
F+ NNA R+F K +NKG +F
Sbjct: 319 FIQVNNATQNPRIFLKNINKGQIF 248
>TC8469 similar to UP|Q9MAH9 (Q9MAH9) F12M16.12, partial (48%)
Length = 1039
Score = 25.4 bits (54), Expect = 8.2
Identities = 15/50 (30%), Positives = 24/50 (48%)
Frame = +3
Query: 64 PGSTSNPLGSKVTPVTVEQLFGLNTLGISLARIDFAPKGLNPPHTHPRAT 113
P S+S+ S VTP L + ++ A +P+ NPP + P A+
Sbjct: 48 PSSSSSF*SSAVTPTLASSLLLIRFQTVAGAASFASPRRRNPPVSRPTAS 197
>TC15046 similar to UP|Q9FVN7 (Q9FVN7) NADPH-dependent mannose 6-phosphate
reductase, partial (54%)
Length = 547
Score = 25.4 bits (54), Expect = 8.2
Identities = 12/32 (37%), Positives = 17/32 (52%)
Frame = -2
Query: 90 GISLARIDFAPKGLNPPHTHPRATEILIVLEG 121
GI +DF P LNPPH+ P + ++G
Sbjct: 147 GIENEVLDFLP--LNPPHSEPNDRHLETAVQG 58
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.319 0.138 0.404
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,644,648
Number of Sequences: 28460
Number of extensions: 43612
Number of successful extensions: 209
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 199
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 199
length of query: 223
length of database: 4,897,600
effective HSP length: 87
effective length of query: 136
effective length of database: 2,421,580
effective search space: 329334880
effective search space used: 329334880
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)
Lotus: description of TM0064b.12