BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0061.9
(277 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC17153 UP|AAQ87023 (AAQ87023) VDAC3.1, complete 553 e-158
TC7918 UP|AAQ87019 (AAQ87019) VDAC1.1, complete 263 3e-71
TC8300 UP|AAQ87020 (AAQ87020) VDAC1.2, complete 254 1e-68
TC8287 UP|AAQ87022 (AAQ87022) VDAC2.1, complete 248 1e-66
TC8223 UP|AAQ87021 (AAQ87021) VDAC1.3, complete 243 3e-65
AV772563 43 3e-08
BP038896 27 2.9
AV409225 27 3.8
TC15910 similar to UP|Q9AR64 (Q9AR64) Urease accessory protein G... 27 5.0
AI967626 26 6.5
BP075269 26 6.5
TC19967 26 6.5
AI967493 26 8.5
AI967315 26 8.5
BP046590 26 8.5
>TC17153 UP|AAQ87023 (AAQ87023) VDAC3.1, complete
Length = 1175
Score = 553 bits (1424), Expect = e-158
Identities = 277/277 (100%), Positives = 277/277 (100%)
Frame = +2
Query: 1 MANGPALYPEIGKRARDLLYKDYNFDQKFNLSIPSSTGLGLIATGLKKDQIFIGDINTLY 60
MANGPALYPEIGKRARDLLYKDYNFDQKFNLSIPSSTGLGLIATGLKKDQIFIGDINTLY
Sbjct: 146 MANGPALYPEIGKRARDLLYKDYNFDQKFNLSIPSSTGLGLIATGLKKDQIFIGDINTLY 325
Query: 61 KSGNLTVDVKVDTYSNVSTKVTLNDIFRCKKVALSFNIPDHKSGKLDVQYLHPHAAIDSS 120
KSGNLTVDVKVDTYSNVSTKVTLNDIFRCKKVALSFNIPDHKSGKLDVQYLHPHAAIDSS
Sbjct: 326 KSGNLTVDVKVDTYSNVSTKVTLNDIFRCKKVALSFNIPDHKSGKLDVQYLHPHAAIDSS 505
Query: 121 IGLNPSPQLELSTAIGSKDLCMGAEVGFNTTSASFTKYNAGITFNRPDFSAALMLVDKGK 180
IGLNPSPQLELSTAIGSKDLCMGAEVGFNTTSASFTKYNAGITFNRPDFSAALMLVDKGK
Sbjct: 506 IGLNPSPQLELSTAIGSKDLCMGAEVGFNTTSASFTKYNAGITFNRPDFSAALMLVDKGK 685
Query: 181 SLKASYIHHVDRPDGFTVAAEINHRFSSFENRFTIGSSQLIDPNTVLKTRFSDDGKAAFL 240
SLKASYIHHVDRPDGFTVAAEINHRFSSFENRFTIGSSQLIDPNTVLKTRFSDDGKAAFL
Sbjct: 686 SLKASYIHHVDRPDGFTVAAEINHRFSSFENRFTIGSSQLIDPNTVLKTRFSDDGKAAFL 865
Query: 241 CQRAWRPKSLITLSAEYDSTKIFGSSTKLGLALALQP 277
CQRAWRPKSLITLSAEYDSTKIFGSSTKLGLALALQP
Sbjct: 866 CQRAWRPKSLITLSAEYDSTKIFGSSTKLGLALALQP 976
>TC7918 UP|AAQ87019 (AAQ87019) VDAC1.1, complete
Length = 1601
Score = 263 bits (671), Expect = 3e-71
Identities = 134/277 (48%), Positives = 182/277 (65%)
Frame = +2
Query: 1 MANGPALYPEIGKRARDLLYKDYNFDQKFNLSIPSSTGLGLIATGLKKDQIFIGDINTLY 60
MA GP LY +IGK+ARDLL+KDY+ DQKF ++ S TG+ + ++G KK ++F+ D+NT
Sbjct: 80 MAKGPGLYTDIGKKARDLLFKDYHSDQKFTITTFSPTGVAITSSGTKKGELFVADVNTQL 259
Query: 61 KSGNLTVDVKVDTYSNVSTKVTLNDIFRCKKVALSFNIPDHKSGKLDVQYLHPHAAIDSS 120
K+ N+T D+KVDT SN+ T + +ND K SF +PD +SGK+++QYLH +A I SS
Sbjct: 260 KNKNITTDIKVDTDSNLFTTIAVNDPAPGLKAIFSFKVPDQRSGKVELQYLHDYAGISSS 439
Query: 121 IGLNPSPQLELSTAIGSKDLCMGAEVGFNTTSASFTKYNAGITFNRPDFSAALMLVDKGK 180
IGL +P + S IG+ L +GA++ F+T TK NAG++F + D AAL L DKG
Sbjct: 440 IGLTANPIVNFSGVIGTNVLALGADLTFDTKIGELTKSNAGLSFTKDDLIAALTLNDKGD 619
Query: 181 SLKASYIHHVDRPDGFTVAAEINHRFSSFENRFTIGSSQLIDPNTVLKTRFSDDGKAAFL 240
+L ASY H V V AE+ HRFS+ EN T+G+ +DP T LK R ++ GKA+ L
Sbjct: 620 ALNASYYHVVSPLTNTAVGAEVTHRFSTNENTLTLGTQHALDPLTTLKARVNNFGKASAL 799
Query: 241 CQRAWRPKSLITLSAEYDSTKIFGSSTKLGLALALQP 277
Q WRPKS T+S E D TK S K+GLALAL+P
Sbjct: 800 IQHEWRPKSFFTISGEVD-TKAIEKSAKVGLALALKP 907
>TC8300 UP|AAQ87020 (AAQ87020) VDAC1.2, complete
Length = 1293
Score = 254 bits (648), Expect = 1e-68
Identities = 126/277 (45%), Positives = 181/277 (64%)
Frame = +3
Query: 1 MANGPALYPEIGKRARDLLYKDYNFDQKFNLSIPSSTGLGLIATGLKKDQIFIGDINTLY 60
MA GP LY +IGKRARDLL+KDY+ DQKF ++ S TG+ + ++G+KK ++F+ D+NT
Sbjct: 87 MAKGPGLYTDIGKRARDLLFKDYHSDQKFTITTYSPTGVAITSSGIKKGELFVADVNTQL 266
Query: 61 KSGNLTVDVKVDTYSNVSTKVTLNDIFRCKKVALSFNIPDHKSGKLDVQYLHPHAAIDSS 120
K+ N+T D+KVDT SN+ T VT+++ K SF +PD +SGK+++QYLH +A I +S
Sbjct: 267 KNKNITTDLKVDTDSNLFTTVTVDEPAPGLKAIFSFRVPDQRSGKVELQYLHDYAGISTS 446
Query: 121 IGLNPSPQLELSTAIGSKDLCMGAEVGFNTTSASFTKYNAGITFNRPDFSAALMLVDKGK 180
+GL P+P + S +G+ L +G ++ F+T TK+NAG+ F + D A+L + DKG
Sbjct: 447 VGLTPNPIVNFSGVVGTNALAVGTDLSFDTKIGELTKFNAGLNFTKADLIASLTVNDKGD 626
Query: 181 SLKASYIHHVDRPDGFTVAAEINHRFSSFENRFTIGSSQLIDPNTVLKTRFSDDGKAAFL 240
+L ASY H V+ AE+ HRFS+ EN T+G+ +DP T +K R ++ GKA L
Sbjct: 627 ALNASYYHIVNPLTNTAFGAEVTHRFSTNENTLTLGTQHALDPLTSVKARVNNLGKANAL 806
Query: 241 CQRAWRPKSLITLSAEYDSTKIFGSSTKLGLALALQP 277
Q WR KS T+S E D TK S K+GL LAL+P
Sbjct: 807 IQHEWRAKSFFTISGEVD-TKAIEKSAKIGLGLALKP 914
>TC8287 UP|AAQ87022 (AAQ87022) VDAC2.1, complete
Length = 1194
Score = 248 bits (632), Expect = 1e-66
Identities = 124/277 (44%), Positives = 181/277 (64%)
Frame = +1
Query: 1 MANGPALYPEIGKRARDLLYKDYNFDQKFNLSIPSSTGLGLIATGLKKDQIFIGDINTLY 60
M+ GP L+ +IGK+ RDLL KDY+ DQK +S S+ G+ L +T +KK + +GD+ LY
Sbjct: 91 MSKGPGLFSDIGKKPRDLLTKDYSSDQKLTVSSYSTAGVALTSTAVKKGGLSVGDVAALY 270
Query: 61 KSGNLTVDVKVDTYSNVSTKVTLNDIFRCKKVALSFNIPDHKSGKLDVQYLHPHAAIDSS 120
K N +DVK+DT S +ST +T D+ K SF +PD+ SGKL+VQY H HA + ++
Sbjct: 271 KYKNTLIDVKLDTASIISTTLTFTDLLPSTKAIASFKLPDYNSGKLEVQYFHDHATLTAA 450
Query: 121 IGLNPSPQLELSTAIGSKDLCMGAEVGFNTTSASFTKYNAGITFNRPDFSAALMLVDKGK 180
LN SP +++S G+ + +GAE G++T+S FTKY AGI+ +PD SA+++L DKG
Sbjct: 451 AALNQSPIIDVSATFGTPVIAVGAEAGYDTSSGGFTKYTAGISVTKPDASASIILGDKGD 630
Query: 181 SLKASYIHHVDRPDGFTVAAEINHRFSSFENRFTIGSSQLIDPNTVLKTRFSDDGKAAFL 240
S+KASY+HH+D+ AEI +FS+ EN FT+G S ID T ++ R ++ GK L
Sbjct: 631 SIKASYLHHLDQLKKSAAVAEITRKFSTNENTFTVGGSFAIDHLTQVRARLNNHGKLGAL 810
Query: 241 CQRAWRPKSLITLSAEYDSTKIFGSSTKLGLALALQP 277
Q PKS++T+S+E D TK + + GLA+AL+P
Sbjct: 811 LQHEIIPKSVLTISSEVD-TKALDKNPRFGLAIALKP 918
>TC8223 UP|AAQ87021 (AAQ87021) VDAC1.3, complete
Length = 1261
Score = 243 bits (620), Expect = 3e-65
Identities = 126/277 (45%), Positives = 178/277 (63%)
Frame = +2
Query: 1 MANGPALYPEIGKRARDLLYKDYNFDQKFNLSIPSSTGLGLIATGLKKDQIFIGDINTLY 60
M GP LY +IGK+ARDLL+KDY D KF ++ +S G+ + +TG+KK +I++ D++T
Sbjct: 29 MVKGPGLYSDIGKKARDLLFKDYQNDHKFTITTYTSAGVEITSTGVKKGEIYLADVSTKL 208
Query: 61 KSGNLTVDVKVDTYSNVSTKVTLNDIFRCKKVALSFNIPDHKSGKLDVQYLHPHAAIDSS 120
K+ N+T DVKVDT SN+ T +T+++ K SF PD KSGK+++QY H +A I +S
Sbjct: 209 KNKNITTDVKVDTNSNLHTTITVDEPAPGLKTIFSFIFPDQKSGKVELQYQHEYAGISTS 388
Query: 121 IGLNPSPQLELSTAIGSKDLCMGAEVGFNTTSASFTKYNAGITFNRPDFSAALMLVDKGK 180
IGL SP + S IG+ + +G ++ F+T S +FTKYNAG+ D A+L L DKG
Sbjct: 389 IGLTASPIVNFSGIIGNNLVAVGTDLSFDTASGNFTKYNAGLNVTHADLIASLTLNDKGD 568
Query: 181 SLKASYIHHVDRPDGFTVAAEINHRFSSFENRFTIGSSQLIDPNTVLKTRFSDDGKAAFL 240
SL ASY H V V AE++H FSS EN TIG+ +DP T+LK R ++ G+A+ L
Sbjct: 569 SLNASYYHVVSPLTNTAVGAELSHSFSSNENILTIGTQHALDPITLLKARVNNYGRASAL 748
Query: 241 CQRAWRPKSLITLSAEYDSTKIFGSSTKLGLALALQP 277
Q W P++ ++L E D T S K+GLALAL+P
Sbjct: 749 IQHEWNPRTRVSLVGEVD-TGAIEKSAKVGLALALKP 856
>AV772563
Length = 477
Score = 43.1 bits (100), Expect(2) = 3e-08
Identities = 20/48 (41%), Positives = 28/48 (57%)
Frame = -3
Query: 204 HRFSSFENRFTIGSSQLIDPNTVLKTRFSDDGKAAFLCQRAWRPKSLI 251
HRFS EN T+G+ +DP T +K R + G+A Q WR +SL+
Sbjct: 475 HRFSIHENTLTLGTQHALDPLTSVKARVNHLGQANAFIQHEWRAQSLL 332
Score = 30.4 bits (67), Expect(2) = 3e-08
Identities = 17/31 (54%), Positives = 18/31 (57%)
Frame = -1
Query: 247 PKSLITLSAEYDSTKIFGSSTKLGLALALQP 277
P TLS E D TK S K+GL LALQP
Sbjct: 345 PSHFFTLSGEGD-TKALEKSAKIGLGLALQP 256
>BP038896
Length = 518
Score = 27.3 bits (59), Expect = 2.9
Identities = 13/32 (40%), Positives = 19/32 (58%)
Frame = -1
Query: 78 STKVTLNDIFRCKKVALSFNIPDHKSGKLDVQ 109
++KVTL +I CK+ + DH SGK+ Q
Sbjct: 251 TSKVTLTEIIHCKRYD---ELEDHYSGKMKTQ 165
>AV409225
Length = 416
Score = 26.9 bits (58), Expect = 3.8
Identities = 9/29 (31%), Positives = 20/29 (68%)
Frame = +2
Query: 204 HRFSSFENRFTIGSSQLIDPNTVLKTRFS 232
HR SS+ N+ T ++ +DP+T+ ++ ++
Sbjct: 194 HRLSSYHNKCTSNATICLDPSTIFESFYT 280
>TC15910 similar to UP|Q9AR64 (Q9AR64) Urease accessory protein G, partial
(88%)
Length = 1061
Score = 26.6 bits (57), Expect = 5.0
Identities = 41/170 (24%), Positives = 66/170 (38%), Gaps = 3/170 (1%)
Frame = +2
Query: 13 KRARDLLYKDYNFDQKFNLSIPSSTGLGLIATGLKKDQIFIGDINTLYKSGNLTVDVKVD 72
+RA L+ +D+N ++ F + I G G A L + F+ D
Sbjct: 221 RRAPPLINRDFN-ERAFTIGIGGPVGTGKTALMLALCE-FLRD----------------- 343
Query: 73 TYSNVSTKVTLNDIFRCKK---VALSFNIPDHKSGKLDVQYLHPHAAIDSSIGLNPSPQL 129
N S NDIF + + + +P+ + ++ PHAAI I +N P
Sbjct: 344 ---NYSLAAVTNDIFTKEDGEFLVRNKALPEERIRAVETGGC-PHAAIREDISINLGPLE 511
Query: 130 ELSTAIGSKDLCMGAEVGFNTTSASFTKYNAGITFNRPDFSAALMLVDKG 179
ELS + L E G + +A+F++ A D S + KG
Sbjct: 512 ELSNLYKTDILL--CESGGDNLAANFSRELADYIIYIIDVSGGDKIPRKG 655
>AI967626
Length = 386
Score = 26.2 bits (56), Expect = 6.5
Identities = 14/34 (41%), Positives = 19/34 (55%), Gaps = 4/34 (11%)
Frame = +3
Query: 233 DDGKAAFLCQRAWRPKSLI----TLSAEYDSTKI 262
DDG FLC+R +R I TL+ E D T++
Sbjct: 75 DDGVCGFLCRRQFRSFIFISFVPTLAREIDRTRL 176
>BP075269
Length = 218
Score = 26.2 bits (56), Expect = 6.5
Identities = 16/59 (27%), Positives = 30/59 (50%), Gaps = 2/59 (3%)
Frame = +3
Query: 66 TVDVKVDTYSNVSTKVTLNDIFRCKKVALSFNIPDHK--SGKLDVQYLHPHAAIDSSIG 122
T+ K +T N +T+ +I + ++IP+ + SGK D++Y H + S+G
Sbjct: 63 TISSKNNTMDNNKLPITIQNI-------IDYSIPEARLRSGKPDLRYESAHPKSNRSVG 218
>TC19967
Length = 520
Score = 26.2 bits (56), Expect = 6.5
Identities = 12/38 (31%), Positives = 21/38 (54%)
Frame = -2
Query: 83 LNDIFRCKKVALSFNIPDHKSGKLDVQYLHPHAAIDSS 120
++ F C+ V L++ + S V+Y+ P AID+S
Sbjct: 513 VDKFFNCEDVLLNYLYANASSSSRTVEYVKPAWAIDTS 400
>AI967493
Length = 345
Score = 25.8 bits (55), Expect = 8.5
Identities = 12/40 (30%), Positives = 22/40 (55%)
Frame = -2
Query: 223 PNTVLKTRFSDDGKAAFLCQRAWRPKSLITLSAEYDSTKI 262
PN L+ +F+ +RAW+PK T+S E++ ++
Sbjct: 134 PNLHLRVQFATPQ------ERAWKPKRETTMSVEHELQRV 33
>AI967315
Length = 1308
Score = 25.8 bits (55), Expect = 8.5
Identities = 8/17 (47%), Positives = 13/17 (76%)
Frame = +1
Query: 22 DYNFDQKFNLSIPSSTG 38
+Y +D F++S+P STG
Sbjct: 1045 EYEYDSSFSMSLPDSTG 1095
>BP046590
Length = 498
Score = 25.8 bits (55), Expect = 8.5
Identities = 11/29 (37%), Positives = 14/29 (47%)
Frame = +2
Query: 101 HKSGKLDVQYLHPHAAIDSSIGLNPSPQL 129
H +G D +Y HP D + L P P L
Sbjct: 122 HHNGNQDNEYKHPSTHNDFDVSLFPPPTL 208
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.318 0.135 0.387
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,169,462
Number of Sequences: 28460
Number of extensions: 50818
Number of successful extensions: 200
Number of sequences better than 10.0: 30
Number of HSP's better than 10.0 without gapping: 197
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 197
length of query: 277
length of database: 4,897,600
effective HSP length: 89
effective length of query: 188
effective length of database: 2,364,660
effective search space: 444556080
effective search space used: 444556080
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 54 (25.4 bits)
Lotus: description of TM0061.9