BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0060.7
(268 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC8428 similar to UP|Q9SNY0 (Q9SNY0) SCUTL2, partial (87%) 221 8e-59
TC20023 similar to GB|AAP13435.1|30023804|BT006327 At1g20030 {Ar... 163 3e-41
TC14162 similar to UP|OLP1_LYCES (Q41350) Osmotin-like protein p... 150 2e-37
TC9212 similar to UP|TLPH_ARATH (P50699) Thaumatin-like protein ... 133 3e-32
TC7964 homologue to UP|O81926 (O81926) Thaumatin-like protein PR... 127 3e-30
TC19049 similar to PIR|T05694|T05694 pathogenesis-related protei... 124 1e-29
TC18115 weakly similar to UP|Q93XD4 (Q93XD4) Thaumatin-like prot... 107 2e-24
BP046128 94 2e-20
TC16995 similar to PIR|T05493|T05493 pathogenesis-related protei... 91 2e-19
TC7965 homologue to UP|O81926 (O81926) Thaumatin-like protein PR... 77 2e-15
BP036640 60 5e-10
AV769280 52 1e-07
BP056986 51 2e-07
AV409685 49 7e-07
AV410524 42 8e-05
AW428867 33 0.066
TC17490 similar to GB|AAO64817.1|29028876|BT005882 At3g04570 {Ar... 30 0.33
TC17620 weakly similar to GB|AAL49941.1|17979249|AY070475 AT3g04... 30 0.56
TC14302 similar to UP|Y230_ARATH (O80934) Protein At2g37660, chl... 27 2.8
AV413391 27 2.8
>TC8428 similar to UP|Q9SNY0 (Q9SNY0) SCUTL2, partial (87%)
Length = 1131
Score = 221 bits (564), Expect = 8e-59
Identities = 102/190 (53%), Positives = 125/190 (65%), Gaps = 4/190 (2%)
Frame = +1
Query: 59 GQSVKLTTVPGWSGRIWARTGCKFDATGVGKCQTGDCGGRLECEGSGAAPPTSLFEITLG 118
G SV++T GWSGR WARTGC FD TG G C TGDC G L+C G G PP +L E T+G
Sbjct: 235 GASVQVTAPSGWSGRFWARTGCNFDGTGNGNCATGDCAGGLKCTGGGV-PPVTLAEFTIG 411
Query: 119 --AGGGQDFYDVSMVDGYNLPLVALPRGAYSGA--TGCMTDINRGCPKELQVVGGDGNQS 174
+ G +DFYDVS+VDGYN+ + G GC+ D+N CP ELQV G +
Sbjct: 412 TASNGNKDFYDVSLVDGYNVGMGVRATGGTGDCQYAGCVADLNGSCPAELQVKGENSTGE 591
Query: 175 VVGCRSACEALKLDKYCCSGEFANPQTCQPSYYSTIFKRACPSAYSYAFDDGTSTFTCKA 234
VV C+SAC A ++CC+GE A P TC P++YS IFK ACP+AYSYA+DD TST TC
Sbjct: 592 VVACKSACAAFNTTEFCCTGEHATPATCSPTHYSEIFKSACPTAYSYAYDDATSTCTCSG 771
Query: 235 NEYDIVFCPN 244
++Y I FCPN
Sbjct: 772 SDYSITFCPN 801
>TC20023 similar to GB|AAP13435.1|30023804|BT006327 At1g20030 {Arabidopsis
thaliana;}, partial (46%)
Length = 418
Score = 163 bits (412), Expect = 3e-41
Identities = 75/136 (55%), Positives = 96/136 (70%), Gaps = 3/136 (2%)
Frame = +3
Query: 97 GRLECEGSGAAPPTSLFEITLGAGGGQDFYDVSMVDGYNLPLVALPRGAYSG---ATGCM 153
G+LEC GSGAAPP +L E TL G DF+DVS+VDGYN+P++ +P G ATGC+
Sbjct: 12 GKLECAGSGAAPPATLAEFTLDGSDGLDFFDVSLVDGYNVPMLVVPEGGSGNNCTATGCV 191
Query: 154 TDINRGCPKELQVVGGDGNQSVVGCRSACEALKLDKYCCSGEFANPQTCQPSYYSTIFKR 213
D+N CP EL+V DG ++V C+SACEA +YCCSG + +P TC+PS YS +FK
Sbjct: 192 GDLNGACPSELRVTSVDGKRNVA-CKSACEAFGSPQYCCSGAYGSPNTCKPSTYSQVFKS 368
Query: 214 ACPSAYSYAFDDGTST 229
ACP AYSYA+DD +ST
Sbjct: 369 ACPRAYSYAYDDKSST 416
>TC14162 similar to UP|OLP1_LYCES (Q41350) Osmotin-like protein precursor,
partial (87%)
Length = 1232
Score = 150 bits (380), Expect = 2e-37
Identities = 74/178 (41%), Positives = 101/178 (56%), Gaps = 5/178 (2%)
Frame = +1
Query: 70 WSGRIWARTGCKFDATGVGKCQTGDCGGRLECEGSGAAPPTSLFEITLGAGGGQDF--YD 127
WSGR+WARTGC + A C +GDCGGRL+C G+G + P +L + + G DF Y
Sbjct: 250 WSGRVWARTGCTYAAGNRFTCASGDCGGRLQCAGAGGSAPATLAQFDMHHGNA-DFSSYS 426
Query: 128 VSMVDGYNLPLVALPRGAYS--GATGCMTDINRGCPKELQVVGGDGNQSVVGCRSACEAL 185
VS+VDG+N+P+ P GC +D+ CP L + VV C+S CEA
Sbjct: 427 VSLVDGFNVPMTVTPHEGKGVCPVVGCRSDLLATCPPVLHHRVPAVHGPVVACKSGCEAF 606
Query: 186 KLDKYCCSGEFANPQTCQPSYYSTIFKRACPSAYSYAFDDGTSTFTCKA-NEYDIVFC 242
D+ CC F N QTC+PS YST FK ACP+ +++A D + T C + E ++FC
Sbjct: 607 HSDELCCRNHFNNAQTCKPSVYSTFFKHACPATFTFAHDTPSLTHECSSPRELKVIFC 780
>TC9212 similar to UP|TLPH_ARATH (P50699) Thaumatin-like protein precursor,
partial (51%)
Length = 662
Score = 133 bits (335), Expect = 3e-32
Identities = 67/124 (54%), Positives = 82/124 (66%), Gaps = 3/124 (2%)
Frame = +1
Query: 124 DFYDVSMVDGYNLPLVALP---RGAYSGATGCMTDINRGCPKELQVVGGDGNQSVVGCRS 180
DFYDVS+VDGYNLP+ P G S A GC+ D+N CP LQV D N+ VV C+S
Sbjct: 1 DFYDVSLVDGYNLPISITPLKGSGKCSYA-GCVRDLNIMCPVGLQVRSRD-NKRVVACKS 174
Query: 181 ACEALKLDKYCCSGEFANPQTCQPSYYSTIFKRACPSAYSYAFDDGTSTFTCKANEYDIV 240
AC A +YCC+G + +PQ+C+P+ YS IFK ACP AYSYA+DD TS TC Y I
Sbjct: 175 ACAAFNSPRYCCTGSYGSPQSCKPTAYSRIFKTACPKAYSYAYDDPTSISTCTHASYLIT 354
Query: 241 FCPN 244
FCP+
Sbjct: 355 FCPH 366
>TC7964 homologue to UP|O81926 (O81926) Thaumatin-like protein PR-5b
precursor (Fragment), partial (74%)
Length = 778
Score = 127 bits (318), Expect = 3e-30
Identities = 62/143 (43%), Positives = 78/143 (54%)
Frame = +2
Query: 73 RIWARTGCKFDATGVGKCQTGDCGGRLECEGSGAAPPTSLFEITLGAGGGQDFYDVSMVD 132
RIW RTGC FD G G+CQTGDC G L C+G G PP +L E L G QDFYD+S+VD
Sbjct: 230 RIWGRTGCNFDGAGRGRCQTGDCTGGLNCQGWGV-PPNTLAEFALNQYGNQDFYDISLVD 406
Query: 133 GYNLPLVALPRGAYSGATGCMTDINRGCPKELQVVGGDGNQSVVGCRSACEALKLDKYCC 192
G+N+P+ P C DIN CP EL+ G GC + C A K +++CC
Sbjct: 407 GFNIPMDFFPLNGGCHKISCTADINGQCPNELRAPG--------GCNNPCTAYKTNEFCC 562
Query: 193 SGEFANPQTCQPSYYSTIFKRAC 215
+ TC P+ + FK C
Sbjct: 563 TN---GQGTCGPN*FLRFFKDRC 622
>TC19049 similar to PIR|T05694|T05694 pathogenesis-related protein
F20M13.220 - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (36%)
Length = 861
Score = 124 bits (312), Expect = 1e-29
Identities = 63/124 (50%), Positives = 76/124 (60%), Gaps = 9/124 (7%)
Frame = +3
Query: 50 FQRPDSGWTG------QSVKLTTVPGWSGRIWARTGCKFDATGVGKCQTGDCG-GRLECE 102
F +P+ G TG S GWSGR WARTGC+FD++G G C T DCG G + C
Sbjct: 492 FGKPEIGSTGFELTKGSSKSFQAPTGWSGRFWARTGCQFDSSGRGTCATADCGSGEINCN 671
Query: 103 GSGAAPPTSLFEITLGAGGGQDFYDVSMVDGYNLPLVALPRGAYS--GATGCMTDINRGC 160
G+ A PP +L E TLG G DFYDVS+VDGYNLP++ + G ATGC D+NR C
Sbjct: 672 GATALPPATLAEFTLGT-GSMDFYDVSLVDGYNLPMMVVASGGSGSCSATGCGADLNRKC 848
Query: 161 PKEL 164
P EL
Sbjct: 849 PSEL 860
>TC18115 weakly similar to UP|Q93XD4 (Q93XD4) Thaumatin-like protein,
partial (63%)
Length = 792
Score = 107 bits (268), Expect = 2e-24
Identities = 67/187 (35%), Positives = 84/187 (44%), Gaps = 1/187 (0%)
Frame = +2
Query: 58 TGQSVKLTTVPGWSGRIWARTGCKFDATGVGKCQTGDCGGRLECEGSGAAPPTSLFEITL 117
+G++ L G SGRIW RTGC FD +G G CQTGDCGG L C SG PPT+L E TL
Sbjct: 146 SGETWPLNLNAGSSGRIWGRTGCNFDGSGRGSCQTGDCGGALSCSLSG-RPPTTLAEFTL 322
Query: 118 GAGGGQDFYDVSMVDGYNLPLVALPRGAYSGATGCMTDINRGCPKELQVVGGDGNQSVVG 177
G QD+YD+S++DG+N+P+ P + GC + L+
Sbjct: 323 NGGNNQDYYDISVIDGFNVPMEFSP-------------TSNGCTRSLR------------ 427
Query: 178 CRSACEALKLDKYCCSGEFANPQTCQPSYYSTIFKRACPSAYSYAFDDGTSTFTCK-ANE 236
C A K CP AY Y DD T T +C
Sbjct: 428 ----CHADK----------------------------CPDAYLYPSDD-TKTHSCPGGTH 508
Query: 237 YDIVFCP 243
Y +VFCP
Sbjct: 509 YRVVFCP 529
>BP046128
Length = 514
Score = 94.0 bits (232), Expect = 2e-20
Identities = 40/71 (56%), Positives = 49/71 (68%)
Frame = -1
Query: 175 VVGCRSACEALKLDKYCCSGEFANPQTCQPSYYSTIFKRACPSAYSYAFDDGTSTFTCKA 234
VVGC SAC A KYCC+GEF +P+ C+PS Y+ FK+ CP AYSY DD +STF+C
Sbjct: 514 VVGCNSACVAFNEAKYCCTGEFESPELCKPSEYALFFKKKCPLAYSYPSDDESSTFSCSR 335
Query: 235 NEYDIVFCPNT 245
+Y I FCP T
Sbjct: 334 ADYTITFCP*T 302
>TC16995 similar to PIR|T05493|T05493 pathogenesis-related protein 19K4.140
- Arabidopsis thaliana {Arabidopsis thaliana;}, partial
(22%)
Length = 614
Score = 91.3 bits (225), Expect = 2e-19
Identities = 35/58 (60%), Positives = 46/58 (78%)
Frame = +1
Query: 189 KYCCSGEFANPQTCQPSYYSTIFKRACPSAYSYAFDDGTSTFTCKANEYDIVFCPNTN 246
+YCC+G F+NP TC+PS YS +FK ACP +YSYA+DD TSTFTC +Y I FCP+++
Sbjct: 4 EYCCNGAFSNPSTCKPSVYSEMFKSACPESYSYAYDDATSTFTCSGADYTITFCPSSS 177
>TC7965 homologue to UP|O81926 (O81926) Thaumatin-like protein PR-5b
precursor (Fragment), partial (37%)
Length = 633
Score = 77.4 bits (189), Expect = 2e-15
Identities = 39/93 (41%), Positives = 50/93 (52%), Gaps = 1/93 (1%)
Frame = +2
Query: 152 CMTDINRGCPKELQVVGGDGNQSVVGCRSACEALKLDKYCCSGEFANPQTCQPSYYSTIF 211
C DIN CP EL+ GG C + C K +++CC+ TC P+ +S F
Sbjct: 8 CTADINGQCPNELRAPGG--------CNNPCTVYKTNEFCCTN---GQGTCGPTNFSRFF 154
Query: 212 KRACPSAYSYAFDDGTSTFTCKA-NEYDIVFCP 243
K C +YSY DD TSTFTC A + Y +VFCP
Sbjct: 155 KDRCHDSYSYPQDDPTSTFTCPAGSNYKVVFCP 253
>BP036640
Length = 468
Score = 59.7 bits (143), Expect = 5e-10
Identities = 28/63 (44%), Positives = 37/63 (58%), Gaps = 1/63 (1%)
Frame = -2
Query: 182 CEALKLDKYCCSGEFANPQTCQPSYYSTIFKRACPSAYSYAFDDGTSTFTCKA-NEYDIV 240
C K +++CC+ TC P+ +S FK C +YSY DD TSTFTC A + Y +V
Sbjct: 464 CTVYKTNEFCCTN---GQGTCGPTNFSRFFKDRCHDSYSYPQDDPTSTFTCPAGSNYKVV 294
Query: 241 FCP 243
FCP
Sbjct: 293 FCP 285
>AV769280
Length = 327
Score = 52.0 bits (123), Expect = 1e-07
Identities = 19/32 (59%), Positives = 25/32 (77%)
Frame = -3
Query: 215 CPSAYSYAFDDGTSTFTCKANEYDIVFCPNTN 246
CP +YSYA+DD TSTFTC +Y I FCP+++
Sbjct: 325 CPKSYSYAYDDATSTFTCSGADYTITFCPSSS 230
>BP056986
Length = 450
Score = 51.2 bits (121), Expect = 2e-07
Identities = 20/32 (62%), Positives = 24/32 (74%)
Frame = -3
Query: 211 FKRACPSAYSYAFDDGTSTFTCKANEYDIVFC 242
FK ACP++YSYA+DD TS FTC +Y I FC
Sbjct: 445 FKEACPTSYSYAYDDPTSIFTCSGTDYVIAFC 350
>AV409685
Length = 270
Score = 49.3 bits (116), Expect = 7e-07
Identities = 22/45 (48%), Positives = 26/45 (56%), Gaps = 1/45 (2%)
Frame = +2
Query: 58 TGQSVKLTTVPGWSGRIWARTGCKFDATGVGKCQTGDCG-GRLEC 101
TG+S + W GR W RT C D+ G C TGDCG G+LEC
Sbjct: 134 TGESKLIPAPANWGGRFWGRTLCSQDSAGKFTCVTGDCGSGKLEC 268
>AV410524
Length = 402
Score = 42.4 bits (98), Expect = 8e-05
Identities = 20/46 (43%), Positives = 25/46 (53%)
Frame = +3
Query: 38 GPVPSPDLQALDFQRPDSGWTGQSVKLTTVPGWSGRIWARTGCKFD 83
G + PDL+ F+ G + + GWSGR WARTGCKFD
Sbjct: 273 GILGKPDLETTGFELK----RGNTRTIPAPAGWSGRFWARTGCKFD 398
>AW428867
Length = 375
Score = 32.7 bits (73), Expect = 0.066
Identities = 12/19 (63%), Positives = 13/19 (68%)
Frame = +1
Query: 70 WSGRIWARTGCKFDATGVG 88
WSGR W R C FDA+G G
Sbjct: 265 WSGRFWGRHDCTFDASGRG 321
>TC17490 similar to GB|AAO64817.1|29028876|BT005882 At3g04570 {Arabidopsis
thaliana;}, partial (9%)
Length = 604
Score = 30.4 bits (67), Expect = 0.33
Identities = 22/69 (31%), Positives = 29/69 (41%)
Frame = +1
Query: 83 DATGVGKCQTGDCGGRLECEGSGAAPPTSLFEITLGAGGGQDFYDVSMVDGYNLPLVALP 142
D G T GG GSG++PP I +G+GGG + D ++PL LP
Sbjct: 70 DDEGPSGAATAASGG-----GSGSSPPPG---IGIGSGGGHQLQAGLVPDPSSMPLYNLP 225
Query: 143 RGAYSGATG 151
S G
Sbjct: 226 PNLLSNGGG 252
>TC17620 weakly similar to GB|AAL49941.1|17979249|AY070475
AT3g04610/F7O18_9 {Arabidopsis thaliana;}, partial
(13%)
Length = 422
Score = 29.6 bits (65), Expect = 0.56
Identities = 20/53 (37%), Positives = 23/53 (42%), Gaps = 7/53 (13%)
Frame = +1
Query: 23 FLP-HSPYSTTAPILYGPVPSPDLQALDFQRPDS------GWTGQSVKLTTVP 68
FLP HSP +T PIL G P + P+S GW G V VP
Sbjct: 10 FLPFHSPMATPDPILNGTTSQPLTEPPPAAEPESADKRWPGWPGHCVYRIIVP 168
>TC14302 similar to UP|Y230_ARATH (O80934) Protein At2g37660, chloroplast
precursor, partial (75%)
Length = 1066
Score = 27.3 bits (59), Expect = 2.8
Identities = 19/52 (36%), Positives = 27/52 (51%), Gaps = 8/52 (15%)
Frame = -2
Query: 8 SSLVPLFCLVSSLALFLPHSPYSTTAPILY--------GPVPSPDLQALDFQ 51
SS P+FCL+SS+ L P + Y + +LY PV S L A+D +
Sbjct: 201 SSAPPIFCLLSSV-LTRPLATYCLSFSVLYTICPVRPPAPVTSTVLGAMDLE 49
>AV413391
Length = 428
Score = 27.3 bits (59), Expect = 2.8
Identities = 20/78 (25%), Positives = 30/78 (37%)
Frame = +1
Query: 22 LFLPHSPYSTTAPILYGPVPSPDLQALDFQRPDSGWTGQSVKLTTVPGWSGRIWARTGCK 81
L P +P+S +P P+P + +Q P T + + P W T C
Sbjct: 49 LLSPPTPHSPPSPAAPSKRPTPSPPRISYQPP*ES-TAATTRWWRTPAW-------TPCT 204
Query: 82 FDATGVGKCQTGDCGGRL 99
F + + CGGRL
Sbjct: 205 FPS-----LRASTCGGRL 243
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.321 0.139 0.453
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,030,730
Number of Sequences: 28460
Number of extensions: 99034
Number of successful extensions: 590
Number of sequences better than 10.0: 51
Number of HSP's better than 10.0 without gapping: 566
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 573
length of query: 268
length of database: 4,897,600
effective HSP length: 89
effective length of query: 179
effective length of database: 2,364,660
effective search space: 423274140
effective search space used: 423274140
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 54 (25.4 bits)
Lotus: description of TM0060.7