Lotus
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= TM0060.17
         (128 letters)

Database: LJGI 
           28,460 sequences; 14,692,800 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

TC15619 similar to UP|Q8GTX9 (Q8GTX9) Cell cycle control crn (Cr...   211  2e-56
TC18715 similar to UP|Q8GTX9 (Q8GTX9) Cell cycle control crn (Cr...    87  1e-18
TC9617                                                                 30  0.17
TC16027                                                                27  1.4
TC19566 similar to UP|O82084 (O82084) MADS-box protein 1, partia...    25  5.4
AV417887                                                               25  5.4
TC7805 homologue to PIR|PQ0772|PQ0772 4-coumarate-CoA ligase  (c...    25  5.4
TC15360 similar to UP|R36A_ARATH (O80929) 60S ribosomal protein ...    25  5.4
BP061158                                                               25  5.4
AV414780                                                               24  7.0
TC11212 similar to UP|Q84WJ9 (Q84WJ9) At5g19680, partial (38%)         24  7.0
TC17369 similar to UP|Q8W558 (Q8W558) 4-coumarate:CoA ligase  , ...    24  9.1

>TC15619 similar to UP|Q8GTX9 (Q8GTX9) Cell cycle control crn (Crooked neck)
           protein-like, partial (26%)
          Length = 822

 Score =  211 bits (537), Expect(2) = 2e-56
 Identities = 104/114 (91%), Positives = 108/114 (94%)
 Frame = +3

Query: 11  QKFSFANMWLLAAQFEIRQLNLKGAHVILGSAIGKAPKDKILKKYIEIELQLGNIDRCRK 70
           +KFSFA MWLLAAQFEIRQLNL GA +ILG+AIGKAPKDKI KKYIEIELQLGNIDRCRK
Sbjct: 234 KKFSFAKMWLLAAQFEIRQLNLTGARLILGNAIGKAPKDKIFKKYIEIELQLGNIDRCRK 413

Query: 71  LYEKYLEWALENCYAWSKYA*LERSLSETDRARSIFELAISQPASDMPELLWKV 124
           LYEKYLEWA ENCYAWSKYA*LERSLSETDRARSIFELAI+QPASDMPEL WKV
Sbjct: 414 LYEKYLEWAPENCYAWSKYA*LERSLSETDRARSIFELAIAQPASDMPELCWKV 575



 Score = 27.3 bits (59), Expect = 0.83
 Identities = 19/67 (28%), Positives = 29/67 (42%), Gaps = 11/67 (16%)
 Frame = +3

Query: 52  LKKYIEIELQLGNIDRCRKLYEK----------YLEWALENCYAWSKYA*LER-SLSETD 100
           L  YI +E  +GN +R R +YE+           L W     Y W  YA  E     + +
Sbjct: 12  LVDYIRLEESVGNKERVRDVYERAIANVPPAEEKLYWQ-RYIYLWINYALYEELDAGDVE 188

Query: 101 RARSIFE 107
           R R +++
Sbjct: 189 RTREVYK 209



 Score = 22.3 bits (46), Expect(2) = 2e-56
 Identities = 10/12 (83%), Positives = 11/12 (91%)
 Frame = +2

Query: 1   LQGVSTLDTSQK 12
           +QGVS LDTSQK
Sbjct: 203 IQGVS*LDTSQK 238


>TC18715 similar to UP|Q8GTX9 (Q8GTX9) Cell cycle control crn (Crooked neck)
           protein-like, partial (6%)
          Length = 889

 Score = 86.7 bits (213), Expect = 1e-18
 Identities = 47/67 (70%), Positives = 51/67 (75%)
 Frame = +3

Query: 2   QGVSTLDTSQKFSFANMWLLAAQFEIRQLNLKGAHVILGSAIGKAPKDKILKKYIEIELQ 61
           QG+S LDTSQKFSFA MWLLAAQFEIRQLNLK A VI G+ IGKAPKDKI KK I     
Sbjct: 402 QGLS*LDTSQKFSFARMWLLAAQFEIRQLNLKDARVISGNDIGKAPKDKIEKKVIPNGDY 581

Query: 62  LGNIDRC 68
             +I+ C
Sbjct: 582 PDDIEEC 602


>TC9617 
          Length = 615

 Score = 29.6 bits (65), Expect = 0.17
 Identities = 20/63 (31%), Positives = 30/63 (46%)
 Frame = +2

Query: 9   TSQKFSFANMWLLAAQFEIRQLNLKGAHVILGSAIGKAPKDKILKKYIEIELQLGNIDRC 68
           TSQ+F     WLL   F    L+L+   + L +++  A    +L KY +   +L N   C
Sbjct: 71  TSQRFKHPTRWLLPVNFPKHLLDLRIVLIALQTSLR*AQMLSLLMKY-QQGRRLANAHLC 247

Query: 69  RKL 71
           R L
Sbjct: 248 RAL 256


>TC16027 
          Length = 653

 Score = 26.6 bits (57), Expect = 1.4
 Identities = 21/73 (28%), Positives = 36/73 (48%), Gaps = 11/73 (15%)
 Frame = +2

Query: 17  NMWLLAAQFEIRQLN--------LKGAHVILGSAIGKAPK---DKILKKYIEIELQLGNI 65
           NM+LL  Q + +Q N        +      LG   G+  K   D  L++Y+E   +  N+
Sbjct: 188 NMFLLRRQTQNQQENGPAQQDAKVAELREALGPLSGRRLKYCTDACLRRYLEA--RNWNV 361

Query: 66  DRCRKLYEKYLEW 78
           D+ +K+ E+ L+W
Sbjct: 362 DKSKKMLEETLKW 400


>TC19566 similar to UP|O82084 (O82084) MADS-box protein 1, partial (17%)
          Length = 531

 Score = 24.6 bits (52), Expect = 5.4
 Identities = 17/48 (35%), Positives = 23/48 (47%), Gaps = 3/48 (6%)
 Frame = -3

Query: 74  KYLEWALENCYAW---SKYA*LERSLSETDRARSIFELAISQPASDMP 118
           K++ W L   YAW    K A L+ S+ E     S+ EL   QP+   P
Sbjct: 388 KHMAWCLGRVYAWI*PDKQAWLKASIQE--*THSLVELEWWQPSDLKP 251


>AV417887 
          Length = 421

 Score = 24.6 bits (52), Expect = 5.4
 Identities = 17/46 (36%), Positives = 23/46 (49%), Gaps = 4/46 (8%)
 Frame = +1

Query: 4   VSTLDTSQKFSFAN---MWLL-AAQFEIRQLNLKGAHVILGSAIGK 45
           V+T + + KF   N    W L + +    +L L GAH  LGS I K
Sbjct: 91  VATGNKNSKFGIRNEKLQWFLDSVKAHPNELKLVGAHCHLGSTITK 228


>TC7805 homologue to PIR|PQ0772|PQ0772 4-coumarate-CoA ligase  (clone
           GM4CL1B) -                soybean (fragment) {Glycine
           max;}  , partial (41%)
          Length = 965

 Score = 24.6 bits (52), Expect = 5.4
 Identities = 11/23 (47%), Positives = 16/23 (68%)
 Frame = +3

Query: 42  AIGKAPKDKILKKYIEIELQLGN 64
           AI K+P  KIL+K +  +L+ GN
Sbjct: 549 AIPKSPSGKILRKDLRAKLENGN 617


>TC15360 similar to UP|R36A_ARATH (O80929) 60S ribosomal protein L36-1,
           partial (88%)
          Length = 534

 Score = 24.6 bits (52), Expect = 5.4
 Identities = 9/20 (45%), Positives = 14/20 (70%)
 Frame = -2

Query: 6   TLDTSQKFSFANMWLLAAQF 25
           TLD S +F FA +W+ + +F
Sbjct: 338 TLDISSRFRFARLWVPSFRF 279


>BP061158 
          Length = 293

 Score = 24.6 bits (52), Expect = 5.4
 Identities = 13/22 (59%), Positives = 15/22 (68%), Gaps = 1/22 (4%)
 Frame = +3

Query: 10  SQKFSFANMWLLAAQF-EIRQL 30
           ++  SFA MWLLAA    IRQL
Sbjct: 168 TKSXSFAKMWLLAAHI*TIRQL 233


>AV414780 
          Length = 294

 Score = 24.3 bits (51), Expect = 7.0
 Identities = 8/20 (40%), Positives = 14/20 (70%)
 Frame = +1

Query: 55 YIEIELQLGNIDRCRKLYEK 74
          ++ + LQ+G +D  RKL E+
Sbjct: 46 FVTLHLQIGQLDEARKLAER 105


>TC11212 similar to UP|Q84WJ9 (Q84WJ9) At5g19680, partial (38%)
          Length = 518

 Score = 24.3 bits (51), Expect = 7.0
 Identities = 10/18 (55%), Positives = 13/18 (71%)
 Frame = -3

Query: 43  IGKAPKDKILKKYIEIEL 60
           +GKA   K+L+KYI I L
Sbjct: 411 LGKAEAKKLLRKYIRINL 358


>TC17369 similar to UP|Q8W558 (Q8W558) 4-coumarate:CoA ligase  , partial
           (20%)
          Length = 580

 Score = 23.9 bits (50), Expect = 9.1
 Identities = 14/39 (35%), Positives = 20/39 (50%), Gaps = 8/39 (20%)
 Frame = +2

Query: 42  AIGKAPKDKILKKYIEIELQLG--------NIDRCRKLY 72
           AI K+P  KIL+K +  +L  G        NID C  ++
Sbjct: 248 AIPKSPSGKILRKNLRSKLVAGVPI*NPLFNIDPCYMMF 364


  Database: LJGI
    Posted date:  Jul 30, 2004 11:16 AM
  Number of letters in database: 14,692,800
  Number of sequences in database:  28,460
  
Lambda     K      H
   0.325    0.138    0.417 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,188,698
Number of Sequences: 28460
Number of extensions: 23358
Number of successful extensions: 129
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 128
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 129
length of query: 128
length of database: 4,897,600
effective HSP length: 80
effective length of query: 48
effective length of database: 2,620,800
effective search space: 125798400
effective search space used: 125798400
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 50 (23.9 bits)


Lotus: description of TM0060.17