BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0060.15
(372 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC16829 homologue to UP|AAQ65183 (AAQ65183) At5g58020, partial (... 73 7e-14
AV406891 60 6e-13
TC11847 50 6e-07
TC14478 weakly similar to UP|Q94G86 (Q94G86) Beta-1,3-glucanase-... 39 0.001
TC12686 similar to UP|Q9FS24 (Q9FS24) Neoxanthin cleavage enzyme... 39 0.001
BI418540 37 0.007
TC12462 weakly similar to UP|Q8TW78 (Q8TW78) Uncharacterized mem... 33 0.058
TC7994 similar to UP|Q8RU73 (Q8RU73) Ribose-5-phosphate isomeras... 33 0.076
AV409746 33 0.076
TC14636 weakly similar to UP|Q9ZUS6 (Q9ZUS6) At2g37400 protein, ... 33 0.076
TC13393 similar to UP|Q9FMU6 (Q9FMU6) Mitochondrial phosphate tr... 33 0.100
TC8093 homologue to UP|EFTU_TOBAC (P41342) Elongation factor Tu,... 33 0.100
TC10380 similar to UP|Q8S8U2 (Q8S8U2) Ycf1 protein, partial (40%) 32 0.13
TC17922 32 0.13
AV428413 32 0.17
TC15016 weakly similar to UP|Q8L685 (Q8L685) Pherophorin-dz1 pro... 32 0.17
AV409798 32 0.17
AV408760 32 0.17
TC15809 weakly similar to PIR|JN0747|JN0747 histone H1-I - Volvo... 32 0.17
TC8096 weakly similar to GB|AAF89661.1|9622185|AF168782 acinusL ... 32 0.22
>TC16829 homologue to UP|AAQ65183 (AAQ65183) At5g58020, partial (13%)
Length = 422
Score = 73.2 bits (178), Expect = 7e-14
Identities = 46/98 (46%), Positives = 57/98 (57%), Gaps = 27/98 (27%)
Frame = +3
Query: 194 LRIYVQVAVAGLGAHCGVVCDTAAEAGSEDEKEDRWCDSGNR------------------ 235
LR+++QVAVAGLGA + AAEAG ++E +R D G+R
Sbjct: 60 LRVHLQVAVAGLGARRAALASAAAEAGLKNEGGERG-DLGDRERGIVGEGFSMEGEVERG 236
Query: 236 ---------AFQVGEGLIAGDGFVTELLEVQRLDEDLE 264
AFQVGEGLIAGDGFV +L+EV+RLDEDLE
Sbjct: 237 SVV*FREERAFQVGEGLIAGDGFVADLMEVRRLDEDLE 350
>AV406891
Length = 229
Score = 60.5 bits (145), Expect(2) = 6e-13
Identities = 29/31 (93%), Positives = 30/31 (96%)
Frame = -1
Query: 235 RAFQVGEGLIAGDGFVTELLEVQRLDEDLEG 265
RAFQVGEGLIAGDGFV ELLEV+RLDEDLEG
Sbjct: 136 RAFQVGEGLIAGDGFVAELLEVRRLDEDLEG 44
Score = 29.6 bits (65), Expect(2) = 6e-13
Identities = 10/11 (90%), Positives = 11/11 (99%)
Frame = -2
Query: 225 KEDRWCDSGNR 235
KEDRWCD+GNR
Sbjct: 168 KEDRWCDAGNR 136
>TC11847
Length = 460
Score = 50.1 bits (118), Expect = 6e-07
Identities = 26/50 (52%), Positives = 32/50 (64%)
Frame = +1
Query: 1 MALSSFAILAGVTTAVIGALPFFLLPMGGEFPIYIAEAVGLFVAVSMVYA 50
MA FA+LAG T + GALP+F L M GE P YIA VG+F A ++ A
Sbjct: 304 MAQYWFAMLAGFTLGMFGALPWFFLQMEGELPFYIATLVGIFEAALVLCA 453
>TC14478 weakly similar to UP|Q94G86 (Q94G86) Beta-1,3-glucanase-like
protein, partial (83%)
Length = 1784
Score = 39.3 bits (90), Expect = 0.001
Identities = 25/84 (29%), Positives = 38/84 (44%)
Frame = +2
Query: 108 FHNQPPPRTSSFSSFFPRSVSHPSSHNPTNKPLQQRTLSLSLPQRQALTNACPKMATQKR 167
FH Q PP TSS PS + + P+ + + +P+ ++ ++ P AT +
Sbjct: 179 FHRQSPPSTSSDPP--------PSEKSASTAPIPRSSRPSPIPESES--SSAPPTATFQI 328
Query: 168 KQQTPTPKTHKTTPTGYPQNPIKT 191
PTP+ TPT YP P T
Sbjct: 329 SPPIPTPQFSG*TPTSYPTTPPAT 400
>TC12686 similar to UP|Q9FS24 (Q9FS24) Neoxanthin cleavage enzyme, partial
(18%)
Length = 632
Score = 38.9 bits (89), Expect = 0.001
Identities = 29/88 (32%), Positives = 39/88 (43%), Gaps = 2/88 (2%)
Frame = +1
Query: 87 SRIQPQQHKHCKSSPLENDGGFHNQPPPRTSSFSSFFPR--SVSHPSSHNPTNKPLQQRT 144
SRI Q H +S G PPP++++ F R + P S NP P
Sbjct: 196 SRIWAPQVSHQRSQSPSRRG-----PPPQSAAIKPFHARFKHFTSPKSTNPQKPPPPPP- 357
Query: 145 LSLSLPQRQALTNACPKMATQKRKQQTP 172
LPQR+ N CPK+ T RK++ P
Sbjct: 358 -KPPLPQRKTPPNHCPKIGTFYRKRRPP 438
>BI418540
Length = 518
Score = 36.6 bits (83), Expect = 0.007
Identities = 36/123 (29%), Positives = 44/123 (35%), Gaps = 14/123 (11%)
Frame = +1
Query: 80 NPSQPQK-------SRIQPQQHKHCKSSPLE------NDGGFHNQPPPRTSSFSSFFPRS 126
NP QK S+ +P H S P + F PPP S S P S
Sbjct: 7 NPKHAQKP*MR*THSQFKPHTHSLTHSPPCHKWRSSIDLASFSPNPPPSVPSPHS--PSS 180
Query: 127 VSHPSSHNPTNKPLQQRTLSLSLPQRQALTNACPKMATQKRKQQTPTPKTHKTTPTG-YP 185
+ +PSS NP P LSL +P AL A P P TP +
Sbjct: 181 LKNPSSPNPPLTPTPPPPLSLPIPPPAALFTARIGGTPIHAPPPPPAPSPPSATPAAPHC 360
Query: 186 QNP 188
+NP
Sbjct: 361 RNP 369
>TC12462 weakly similar to UP|Q8TW78 (Q8TW78) Uncharacterized membrane
protein specific for M.kandleri, MK-9 family, partial
(6%)
Length = 812
Score = 33.5 bits (75), Expect = 0.058
Identities = 28/97 (28%), Positives = 42/97 (42%), Gaps = 4/97 (4%)
Frame = +3
Query: 97 CKSSPLENDGGFHNQPPPRTSSFSSFFPRSVSHPSSHNPTNKPLQQRTLSLSLPQRQALT 156
C +PL G FH+ P + S RS+ + H T+ P + SLSL + + T
Sbjct: 24 CSRTPLFLPG-FHDSPHDSRTPLLSDHHRSIQSTTKH--TSSPSRLPRFSLSLSRCHSST 194
Query: 157 NACPKMATQKRKQQTP----TPKTHKTTPTGYPQNPI 189
P ++ + + Q P TH TTP P P+
Sbjct: 195 TPPPTLSLKHKLQPAQHHHRRPATHCTTPP--PTQPL 299
>TC7994 similar to UP|Q8RU73 (Q8RU73) Ribose-5-phosphate isomerase
precursor , partial (81%)
Length = 1355
Score = 33.1 bits (74), Expect = 0.076
Identities = 25/77 (32%), Positives = 33/77 (42%), Gaps = 5/77 (6%)
Frame = +2
Query: 64 FGPPVDQAYTPAA----EVRNPSQPQKSRIQPQQHKHCKSSPLENDGGFHNQPPPRTS-S 118
+ PP+ Y P V +PS SR P SSP + PPP +S S
Sbjct: 329 YAPPLPLNYAPPPLPNPSVPSPSPKTTSRDSPPTRPSTTSSPAWSSASAPAPPPPSSSPS 508
Query: 119 FSSFFPRSVSHPSSHNP 135
+SF P + S SS +P
Sbjct: 509 SASFSPPANSPTSSASP 559
>AV409746
Length = 411
Score = 33.1 bits (74), Expect = 0.076
Identities = 23/72 (31%), Positives = 30/72 (40%)
Frame = +3
Query: 110 NQPPPRTSSFSSFFPRSVSHPSSHNPTNKPLQQRTLSLSLPQRQALTNACPKMATQKRKQ 169
+ PPP T + FP+S + P + T KP R S P RQ ++ T
Sbjct: 117 SSPPPSTPPIAFQFPQSPTRPRTPVRTKKPPSHRN---SSPPRQLKSSHSAPTGT----- 272
Query: 170 QTPTPKTHKTTP 181
TPTP T P
Sbjct: 273 STPTPSTPPPVP 308
>TC14636 weakly similar to UP|Q9ZUS6 (Q9ZUS6) At2g37400 protein, partial
(43%)
Length = 1316
Score = 33.1 bits (74), Expect = 0.076
Identities = 18/44 (40%), Positives = 27/44 (60%), Gaps = 1/44 (2%)
Frame = +2
Query: 95 KHCKSSPLENDGGFHNQPPPR-TSSFSSFFPRSVSHPSSHNPTN 137
+H S+P + F +QPPP +SSF S R+ S PS +NP++
Sbjct: 71 RHFPSAPPFSSLSFRSQPPPSFSSSFKSSRIRASSSPSPNNPSS 202
>TC13393 similar to UP|Q9FMU6 (Q9FMU6) Mitochondrial phosphate translocator
(AT5g14040/MUA22_4), partial (8%)
Length = 541
Score = 32.7 bits (73), Expect = 0.100
Identities = 30/96 (31%), Positives = 44/96 (45%), Gaps = 2/96 (2%)
Frame = +1
Query: 100 SPLENDGGFHNQPPPRTSSFSSFFPRSVSHPSSHNPTNKPLQQRTLSLSLPQRQALTNAC 159
+P F N PP RTSS SS P SV PS+ P+ T SL+ P + +
Sbjct: 55 NPPSASSTFPNPPPTRTSSPSS-SPHSVPPPST--PSKSFPITPTGSLAAPAASSSRTSP 225
Query: 160 PKMATQKRKQQTPTPKTHKTTPT--GYPQNPIKTYP 193
P A +P P++ ++PT P+ P+ + P
Sbjct: 226 PSPA----PSLSPNPRSSSSSPTSSASPKTPMISSP 321
>TC8093 homologue to UP|EFTU_TOBAC (P41342) Elongation factor Tu,
chloroplast precursor (EF-Tu), partial (58%)
Length = 1146
Score = 32.7 bits (73), Expect = 0.100
Identities = 26/92 (28%), Positives = 37/92 (39%), Gaps = 8/92 (8%)
Frame = +2
Query: 110 NQPPPRTSSFSSFFPRSVSHPSSHNP------TNKPLQQRTLSLSLPQRQALTNACPKMA 163
N PPP TS S P+S S +NP P L+ S R ++ + P
Sbjct: 203 NYPPP-TSPPPSILPQSSSTRRRNNPPPPSTAAPPPSPSAPLAASSSARNPMSTSAPSAT 379
Query: 164 TQKRKQQTPTPKTHKTTP--TGYPQNPIKTYP 193
+ K +P P + P T P+N K+ P
Sbjct: 380 STTAKPPSPPPSPWPSLPSETAPPRNTTKSTP 475
>TC10380 similar to UP|Q8S8U2 (Q8S8U2) Ycf1 protein, partial (40%)
Length = 5664
Score = 32.3 bits (72), Expect = 0.13
Identities = 22/62 (35%), Positives = 31/62 (49%)
Frame = +1
Query: 253 LLEVQRLDEDLEGKERLGRSGQRWLGLAVHRWLVRKRWGKRKNRKGKGKESRKRKRVGSP 312
L VQ+LD+ E ++LG G++ KRK+ K +GK +KRKR G
Sbjct: 2167 LFYVQKLDKKKERIKKLGLYGRK---------------SKRKSEKRRGKNKKKRKR-GKK 2298
Query: 313 TY 314
TY
Sbjct: 2299 TY 2304
>TC17922
Length = 470
Score = 32.3 bits (72), Expect = 0.13
Identities = 25/86 (29%), Positives = 34/86 (39%), Gaps = 6/86 (6%)
Frame = +1
Query: 112 PPPRTSSFSSFFPRSVSHPSSHNPTNKPLQQRTLSLSLPQRQALTNACPKMATQKRKQQT 171
P TS ++ P S P+S P++ P + T P QA NA P + QT
Sbjct: 91 PTTSTSRSTT*LPPQASPPTSSPPSSPPSRSTT-----PTSQATANAPPYSPNVSTRHQT 255
Query: 172 PT------PKTHKTTPTGYPQNPIKT 191
P+ +H T T P KT
Sbjct: 256 PSGPSSAASTSHSPTNTSSKAAPSKT 333
>AV428413
Length = 416
Score = 32.0 bits (71), Expect = 0.17
Identities = 21/72 (29%), Positives = 38/72 (52%), Gaps = 6/72 (8%)
Frame = +2
Query: 120 SSFFPRSVSHP---SSHNPTNKPLQQRTLSLSL-PQRQALTNACPKMATQKRKQQTPT-- 173
S FF + ++ S+ PT PL + + + P + +LT+ + T ++K++T
Sbjct: 50 SLFFSKQLARKYGASTPTPTPTPLLPAVVEVRISPLKTSLTSLTVTLFTTRKKKRTIHLF 229
Query: 174 PKTHKTTPTGYP 185
P+TH+T PT P
Sbjct: 230 PRTHRTVPTPIP 265
>TC15016 weakly similar to UP|Q8L685 (Q8L685) Pherophorin-dz1 protein
precursor, partial (4%)
Length = 948
Score = 32.0 bits (71), Expect = 0.17
Identities = 26/102 (25%), Positives = 39/102 (37%), Gaps = 1/102 (0%)
Frame = +1
Query: 82 SQPQKSRIQPQQHKHCKSSPLENDGGFHNQPPPRTSSFSSFFPRSVSHPSSH-NPTNKPL 140
+ P + I P + S P QPPP +S SS S PSS +PT K
Sbjct: 49 TSPFPTTITPTKTSSFSSQPTTTTTAAAAQPPPASSPPSSSSSSSPPPPSSSTHPTRKSA 228
Query: 141 QQRTLSLSLPQRQALTNACPKMATQKRKQQTPTPKTHKTTPT 182
+ S + + + + + K T T + TTP+
Sbjct: 229 SSESKSTTSGSESTRSPSSTSRSPSEFKFATGTSSPYPTTPS 354
>AV409798
Length = 411
Score = 32.0 bits (71), Expect = 0.17
Identities = 25/87 (28%), Positives = 42/87 (47%), Gaps = 8/87 (9%)
Frame = +2
Query: 112 PPPRTSSFSSFFPRSVSHPSS-------HNPTNKP-LQQRTLSLSLPQRQALTNACPKMA 163
P PR+ + +FF S+PS+ P N P L+ RTL+ + A N P+
Sbjct: 125 PSPRSCARLNFFSGG-SNPSTPRLQQQQSQPVNSPSLRCRTLA----EAAATVNESPRFQ 289
Query: 164 TQKRKQQTPTPKTHKTTPTGYPQNPIK 190
++ + T +PKT + P++P+K
Sbjct: 290 SKTTPRATKSPKTVAASNPNSPRSPLK 370
>AV408760
Length = 425
Score = 32.0 bits (71), Expect = 0.17
Identities = 21/72 (29%), Positives = 38/72 (52%), Gaps = 6/72 (8%)
Frame = +3
Query: 120 SSFFPRSVSHP---SSHNPTNKPLQQRTLSLSL-PQRQALTNACPKMATQKRKQQTPT-- 173
S FF + ++ S+ PT PL + + + P + +LT+ + T ++K++T
Sbjct: 87 SLFFSKQLARKYGASTPTPTPTPLLPAVVEVRISPLKTSLTSLTVTLFTTRKKKRTIHLF 266
Query: 174 PKTHKTTPTGYP 185
P+TH+T PT P
Sbjct: 267 PRTHRTVPTPIP 302
>TC15809 weakly similar to PIR|JN0747|JN0747 histone H1-I - Volvox carteri
{Volvox carteri;}, partial (10%)
Length = 526
Score = 32.0 bits (71), Expect = 0.17
Identities = 36/122 (29%), Positives = 49/122 (39%), Gaps = 11/122 (9%)
Frame = +3
Query: 78 VRNPSQPQKSRIQPQ--QHKHCKSSPLENDGGFHNQPPPRTSSFSSFFPRSVSHPSSHN- 134
++ P PQ+ QPQ QH H Q PP T+S PRS P S
Sbjct: 168 LQQPQNPQQKHPQPQRLQHHHQNH*SPHYLNPLTPQIPPLTTS-----PRSSGRPKSSQS 332
Query: 135 --PTNKPLQQ---RTLSLSLPQRQALTNACPKMA---TQKRKQQTPTPKTHKTTPTGYPQ 186
+ KPL+ T S SLP+ +A ++ T K+ TP KT K+
Sbjct: 333 *FAS*KPLKS*TTSTHSSSLPRTEA*PSSHQMTLLSHTSKQGSSTP*TKTKKSNSANSTS 512
Query: 187 NP 188
+P
Sbjct: 513 SP 518
>TC8096 weakly similar to GB|AAF89661.1|9622185|AF168782 acinusL protein
{Mus musculus;} , partial (3%)
Length = 1044
Score = 31.6 bits (70), Expect = 0.22
Identities = 23/73 (31%), Positives = 32/73 (43%), Gaps = 3/73 (4%)
Frame = +1
Query: 112 PPPRTSSFSSFFPRSVSHPSSHNPTNKPLQQR---TLSLSLPQRQALTNACPKMATQKRK 168
PPP+ +S S P PSS P P QQR + ++LP L+ A P + +
Sbjct: 37 PPPQAASVS--VPTVAPAPSSSQPEPSPRQQREQHPIPVALPPPPPLSRAPP---VARER 201
Query: 169 QQTPTPKTHKTTP 181
+P P K P
Sbjct: 202 LPSPPPLPEKVDP 240
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.321 0.137 0.432
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,794,714
Number of Sequences: 28460
Number of extensions: 139186
Number of successful extensions: 1353
Number of sequences better than 10.0: 157
Number of HSP's better than 10.0 without gapping: 1304
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1334
length of query: 372
length of database: 4,897,600
effective HSP length: 92
effective length of query: 280
effective length of database: 2,279,280
effective search space: 638198400
effective search space used: 638198400
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 56 (26.2 bits)
Lotus: description of TM0060.15