BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0051b.10
(187 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP049491 261 4e-71
BP042557 184 1e-47
TC19318 similar to UP|Q94JM3 (Q94JM3) AT5g62000/mtg10_20, partia... 160 1e-40
AV410062 80 3e-16
TC10231 similar to UP|O23110 (O23110) AP2 domain containing prot... 44 2e-05
AV409033 37 0.002
AV416874 36 0.004
CB828503 36 0.005
TC13465 similar to UP|O23110 (O23110) AP2 domain containing prot... 31 0.15
TC10047 similar to UP|Q9SGP6 (Q9SGP6) F3M18.8, partial (53%) 30 0.33
TC15287 similar to UP|Q9FMY5 (Q9FMY5) U2 snRNP auxiliary factor,... 29 0.44
BP059539 27 2.8
BP072209 27 2.8
TC9680 homologue to UP|Q9LDY2 (Q9LDY2) Branched chain alpha-keto... 26 3.7
TC17642 similar to UP|SR09_ARATH (Q9SMU7) Signal recognition par... 26 3.7
TC8963 homologue to UP|TBB3_SOYBN (P28551) Tubulin beta chain (F... 26 4.8
TC8645 similar to UP|Q9FKS5 (Q9FKS5) Cytochrome c1, partial (47%) 26 4.8
TC11709 similar to UP|CPCJ_HUMAN (P33261) Cytochrome P450 2C19 ... 25 6.3
TC16467 UP|NUCC_LOTJA (Q9BBN8) NAD(P)H-quinone oxidoreductase ch... 25 8.2
AV779189 25 8.2
>BP049491
Length = 516
Score = 261 bits (668), Expect = 4e-71
Identities = 125/127 (98%), Positives = 126/127 (98%)
Frame = -3
Query: 23 VPRRAAEDCLPPLDYKQQRPSQELVAKDLHGVEWKFCHIYRG*PRWHLLTTGWSIFVSQN 82
VPRRAAEDCLPPLDYKQQRPSQELVAKDLHGVEWKFCHIYRG*PRWHLLTTGWSIFVSQN
Sbjct: 514 VPRRAAEDCLPPLDYKQQRPSQELVAKDLHGVEWKFCHIYRG*PRWHLLTTGWSIFVSQN 335
Query: 83 NLVSGDAVSFLRGENGELRLGIRRSVGPRNGLPESTVGNQNSYPHILSVVANVISTRSMF 142
NLVSGDAVSFLRGENGELRLGIRRSVGPRNGLPESTVGNQNSYPHILSVVAN ISTRSMF
Sbjct: 334 NLVSGDAVSFLRGENGELRLGIRRSVGPRNGLPESTVGNQNSYPHILSVVANAISTRSMF 155
Query: 143 HVFYSPR 149
+VFYSPR
Sbjct: 154 YVFYSPR 134
>BP042557
Length = 545
Score = 184 bits (466), Expect = 1e-47
Identities = 89/165 (53%), Positives = 118/165 (70%)
Frame = +2
Query: 1 STSHMFCKTLTAFDTITHRGFSVPRRAAEDCLPPLDYKQQRPSQELVAKDLHGVEWKFCH 60
+T++ FCKTLTA DT TH GFSVPRRAAE PPLDY QQ P+QEL+A+DL+ EWKF H
Sbjct: 50 TTNNYFCKTLTASDTSTHGGFSVPRRAAEKVFPPLDYSQQPPAQELIARDLNDNEWKFRH 229
Query: 61 IYRG*PRWHLLTTGWSIFVSQNNLVSGDAVSFLRGENGELRLGIRRSVGPRNGLPESTVG 120
I+RG P+ HLLTTGWS+FVS LV+GD+V F+ E +L LGIRR+ + +P S +
Sbjct: 230 IFRGQPKRHLLTTGWSVFVSAKRLVAGDSVLFIWNEKNQLLLGIRRANRSQTIMPSSVLS 409
Query: 121 NQNSYPHILSVVANVISTRSMFHVFYSPRASNSDFVFPYQKYAKS 165
+ + + +L+ A+ ST S F +FY+PRAS S+FV P +Y K+
Sbjct: 410 SDSMHIGLLAAAAHAASTNSRFTIFYNPRASPSEFVIPLAQYVKA 544
>TC19318 similar to UP|Q94JM3 (Q94JM3) AT5g62000/mtg10_20, partial (23%)
Length = 585
Score = 160 bits (405), Expect = 1e-40
Identities = 80/149 (53%), Positives = 104/149 (69%)
Frame = +3
Query: 5 MFCKTLTAFDTITHRGFSVPRRAAEDCLPPLDYKQQRPSQELVAKDLHGVEWKFCHIYRG 64
+ CKTLTA DT T GFSV RR A++CLPPLD +Q P+Q LVAKDLHG EW+F HI+ G
Sbjct: 30 ILCKTLTASDTSTDGGFSVLRRHADECLPPLDMSKQPPTQALVAKDLHGSEWRFRHIFLG 209
Query: 65 *PRWHLLTTGWSIFVSQNNLVSGDAVSFLRGENGELRLGIRRSVGPRNGLPESTVGNQNS 124
PR HLL +GWS+FVS LV+GDA FLRGENGELR+G+RR++ + + S + + +
Sbjct: 210 QPRRHLLLSGWSVFVSSKRLVAGDAFIFLRGENGELRVGVRRAMRQQGNVSSSVISSPSL 389
Query: 125 YPHILSVVANVISTRSMFHVFYSPRASNS 153
+ +L+ + T SMF V+Y PR S S
Sbjct: 390 HLGVLASAWHANLTGSMFTVYY*PRTSPS 476
>AV410062
Length = 435
Score = 79.7 bits (195), Expect = 3e-16
Identities = 36/51 (70%), Positives = 42/51 (81%)
Frame = +3
Query: 3 SHMFCKTLTAFDTITHRGFSVPRRAAEDCLPPLDYKQQRPSQELVAKDLHG 53
++ FCKTLTA DT TH GFSVPRRAAE PPLD+ QQ P+QEL+A+DLHG
Sbjct: 279 TNYFCKTLTASDTSTHGGFSVPRRAAEKVFPPLDFSQQPPAQELIARDLHG 431
>TC10231 similar to UP|O23110 (O23110) AP2 domain containing protein RAP2.8
(Fragment), partial (22%)
Length = 707
Score = 43.9 bits (102), Expect = 2e-05
Identities = 25/70 (35%), Positives = 39/70 (55%), Gaps = 2/70 (2%)
Frame = +1
Query: 49 KDLHGVEWKFCHIYRG*PRWHLLTTGWSIFVSQNNLVSGDAVSFLR--GENGELRLGIRR 106
+D+ G W+F + Y + ++LT GWS FV + NL +GDAV F R G + +L + +
Sbjct: 64 EDVGGKVWRFRYSYWNSSQSYVLTKGWSRFVKEKNLRAGDAVRFHRSTGPDRQLYIDCKA 243
Query: 107 SVGPRNGLPE 116
S G+ E
Sbjct: 244 SSSSGGGVGE 273
>AV409033
Length = 418
Score = 37.0 bits (84), Expect = 0.002
Identities = 16/22 (72%), Positives = 17/22 (76%)
Frame = +3
Query: 3 SHMFCKTLTAFDTITHRGFSVP 24
+ FCKTLTA DT TH GFSVP
Sbjct: 351 TEFFCKTLTASDTSTHGGFSVP 416
>AV416874
Length = 256
Score = 36.2 bits (82), Expect = 0.004
Identities = 16/27 (59%), Positives = 22/27 (81%)
Frame = +2
Query: 81 QNNLVSGDAVSFLRGENGELRLGIRRS 107
+ LV+GD+V FLR ENG+L +GIRR+
Sbjct: 2 RKKLVAGDSVVFLRAENGDLCVGIRRA 82
>CB828503
Length = 487
Score = 35.8 bits (81), Expect = 0.005
Identities = 22/73 (30%), Positives = 34/73 (46%)
Frame = -1
Query: 4 HMFCKTLTAFDTITHRGFSVPRRAAEDCLPPLDYKQQRPSQELVAKDLHGVEWKFCHIYR 63
HMF K +T D +P++ AE P LD L +D +G W+F + Y
Sbjct: 220 HMFDKVVTPSDVGKLNRLVIPKQHAEKYFP-LDSSSNEKGLLLNFEDRNGKLWRFRYSYW 44
Query: 64 G*PRWHLLTTGWS 76
+ +++T GWS
Sbjct: 43 NSSQSYVMTKGWS 5
>TC13465 similar to UP|O23110 (O23110) AP2 domain containing protein RAP2.8
(Fragment), partial (18%)
Length = 609
Score = 30.8 bits (68), Expect = 0.15
Identities = 15/36 (41%), Positives = 22/36 (60%)
Frame = +1
Query: 69 HLLTTGWSIFVSQNNLVSGDAVSFLRGENGELRLGI 104
++LT GWS FV + L +GD+V F R + +L I
Sbjct: 34 YVLTKGWSRFVKEKCLKAGDSVCFHRSTGPDRQLFI 141
>TC10047 similar to UP|Q9SGP6 (Q9SGP6) F3M18.8, partial (53%)
Length = 909
Score = 29.6 bits (65), Expect = 0.33
Identities = 16/50 (32%), Positives = 28/50 (56%), Gaps = 1/50 (2%)
Frame = -2
Query: 94 RGENG-ELRLGIRRSVGPRNGLPESTVGNQNSYPHILSVVANVISTRSMF 142
+G N E+ + +RRS P N P +T GN N++P + +++ + S F
Sbjct: 551 KGTNSPEI*VAMRRSNPPNNLPPINTTGNCNNFPSSIMASNSLVKSTSSF 402
>TC15287 similar to UP|Q9FMY5 (Q9FMY5) U2 snRNP auxiliary factor, small
subunit, partial (81%)
Length = 1263
Score = 29.3 bits (64), Expect = 0.44
Identities = 16/36 (44%), Positives = 23/36 (63%)
Frame = +3
Query: 85 VSGDAVSFLRGENGELRLGIRRSVGPRNGLPESTVG 120
V G+AV + GE G + +G+RR G +NG P S+ G
Sbjct: 774 VIGEAVIGMVGEGGGM-VGVRRGKGAKNGEPGSSNG 878
>BP059539
Length = 515
Score = 26.6 bits (57), Expect = 2.8
Identities = 10/28 (35%), Positives = 18/28 (63%)
Frame = +3
Query: 136 ISTRSMFHVFYSPRASNSDFVFPYQKYA 163
IST ++ +F+ P +SN VFP +++
Sbjct: 342 ISTETLLSLFWXPSSSNHSRVFPAARFS 425
>BP072209
Length = 176
Score = 26.6 bits (57), Expect = 2.8
Identities = 10/17 (58%), Positives = 13/17 (75%)
Frame = -1
Query: 141 MFHVFYSPRASNSDFVF 157
MF FYSPR++ +DF F
Sbjct: 128 MFKPFYSPRSAKTDFXF 78
>TC9680 homologue to UP|Q9LDY2 (Q9LDY2) Branched chain alpha-keto acid
dehydrogenase E1 beta subunit, partial (13%)
Length = 488
Score = 26.2 bits (56), Expect = 3.7
Identities = 17/49 (34%), Positives = 23/49 (46%)
Frame = +1
Query: 122 QNSYPHILSVVANVISTRSMFHVFYSPRASNSDFVFPYQKYAKSIHDNK 170
Q+SY H+L IS + + NS F+FPY YAK+ K
Sbjct: 196 QSSYDHVLG*--GCISVTQIIVL-----GGNSKFIFPYCSYAKNEKSTK 321
>TC17642 similar to UP|SR09_ARATH (Q9SMU7) Signal recognition particle 9
kDa protein (SRP9), partial (54%)
Length = 687
Score = 26.2 bits (56), Expect = 3.7
Identities = 11/27 (40%), Positives = 17/27 (62%), Gaps = 1/27 (3%)
Frame = +2
Query: 54 VEWKFCHIYRG*P-RWHLLTTGWSIFV 79
+E++FCH+Y * WHL +S F+
Sbjct: 20 IEYEFCHLYEA*A*YWHLYKIFYSPFL 100
>TC8963 homologue to UP|TBB3_SOYBN (P28551) Tubulin beta chain (Fragment),
partial (75%)
Length = 985
Score = 25.8 bits (55), Expect = 4.8
Identities = 9/17 (52%), Positives = 12/17 (69%)
Frame = -2
Query: 110 PRNGLPESTVGNQNSYP 126
P+NG PE+ N+NS P
Sbjct: 489 PKNGTPETLASNRNSQP 439
>TC8645 similar to UP|Q9FKS5 (Q9FKS5) Cytochrome c1, partial (47%)
Length = 622
Score = 25.8 bits (55), Expect = 4.8
Identities = 12/32 (37%), Positives = 17/32 (52%)
Frame = -1
Query: 18 HRGFSVPRRAAEDCLPPLDYKQQRPSQELVAK 49
H G P+ LP L YKQ RP+ +++K
Sbjct: 547 H*GHQPPQSPQP*PLPLLQYKQHRPNHGMISK 452
>TC11709 similar to UP|CPCJ_HUMAN (P33261) Cytochrome P450 2C19
((R)-limonene 6-monooxygenase) ((S)-limonene
6-monooxygenase) ((S)-limonene 7-monooxygenase)
(CYPIIC19) (P450-11A) (Mephenytoin 4-hydroxylase)
(CYPIIC17) (P450-254C) , partial (7%)
Length = 473
Score = 25.4 bits (54), Expect = 6.3
Identities = 13/36 (36%), Positives = 19/36 (52%)
Frame = +1
Query: 129 LSVVANVISTRSMFHVFYSPRASNSDFVFPYQKYAK 164
+S+ + S RSM F AS +FP+QK+ K
Sbjct: 259 ISIFNSGTSQRSMVQFFPFDSASRKPSLFPHQKWPK 366
>TC16467 UP|NUCC_LOTJA (Q9BBN8) NAD(P)H-quinone oxidoreductase chain H,
chloroplast (NAD(P)H dehydrogenase, chain H)
(NADH-plastoquinone oxidoreductase 49 kDa subunit) ,
complete
Length = 2407
Score = 25.0 bits (53), Expect = 8.2
Identities = 9/25 (36%), Positives = 13/25 (52%)
Frame = -2
Query: 154 DFVFPYQKYAKSIHDNKRNEPSHRV 178
D P +Y+KS H + P HR+
Sbjct: 1020 DGFLPMNRYSKSFHSGSFSLPKHRI 946
>AV779189
Length = 529
Score = 25.0 bits (53), Expect = 8.2
Identities = 12/34 (35%), Positives = 20/34 (58%)
Frame = +2
Query: 143 HVFYSPRASNSDFVFPYQKYAKSIHDNKRNEPSH 176
++F+S +S+S F K +S N+ NEPS+
Sbjct: 140 NIFFSIPSSSSHFFNTQIKKPRSTTSNEHNEPSN 241
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.323 0.137 0.434
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,681,723
Number of Sequences: 28460
Number of extensions: 51696
Number of successful extensions: 246
Number of sequences better than 10.0: 41
Number of HSP's better than 10.0 without gapping: 246
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 246
length of query: 187
length of database: 4,897,600
effective HSP length: 85
effective length of query: 102
effective length of database: 2,478,500
effective search space: 252807000
effective search space used: 252807000
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 52 (24.6 bits)
Lotus: description of TM0051b.10