Lotus
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= TM0046a.5
         (327 letters)

Database: LJGI 
           28,460 sequences; 14,692,800 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

BP035341                                                               91  3e-19
AV427373                                                               35  0.013
BP061576                                                               34  0.029
TC8858 homologue to UP|Q84N37 (Q84N37) Potyvirus VPg interacting...    32  0.19
TC9614 similar to UP|O22799 (O22799) At2g33490 protein, partial ...    28  2.1
TC9443 similar to UP|Q84N37 (Q84N37) Potyvirus VPg interacting p...    28  2.7
TC16726 similar to UP|GS11_ARATH (Q9LMP7) Golgi SNARE 11 protein...    28  2.7
BP045352                                                               28  2.7
BP051546                                                               27  4.7
TC14602 UP|Q8GS56 (Q8GS56) Phosphoenolpyruvate carboxylase kinas...    27  6.1
TC13257                                                                26  8.0

>BP035341 
          Length = 523

 Score = 90.5 bits (223), Expect = 3e-19
 Identities = 40/72 (55%), Positives = 54/72 (74%), Gaps = 1/72 (1%)
 Frame = -3

Query: 250 SENGSGKTM-YFLAEATHPFYGDSEKELSFSKGDFIVVRKVTPTGWSEGECNGKAGWFPS 308
           + NGS  +M YFL E   P++ +SE EL+ S GD+IV+RKVT  GW+EGEC G+AGWFP 
Sbjct: 458 AHNGSTDSMGYFLGEVLFPYHAESEVELNLSAGDYIVIRKVTNNGWAEGECKGRAGWFPF 279

Query: 309 AYVEKRQRVPSS 320
            Y+E+R+RV +S
Sbjct: 278 GYIERRERVLAS 243


>AV427373 
          Length = 393

 Score = 35.4 bits (80), Expect = 0.013
 Identities = 21/71 (29%), Positives = 32/71 (44%)
 Frame = +3

Query: 75  TAIGYKHIETGTKLSEDCCKYGAENNIDNILAKAASVLGDARKHVEKEHEELNRLLASQV 134
           T  G  H+E  TK+ +D  K   +NN + +   A S L  A            +LLA Q 
Sbjct: 189 TGTGEDHVENATKVKDDGAKTNLDNNFEKLKRAAVSTLAAAAVKA--------KLLADQE 344

Query: 135 LDPLRQMINGV 145
            D +RQ+ + +
Sbjct: 345 EDEIRQLTSSL 377


>BP061576 
          Length = 528

 Score = 34.3 bits (77), Expect = 0.029
 Identities = 24/69 (34%), Positives = 35/69 (49%), Gaps = 1/69 (1%)
 Frame = -2

Query: 165 ETLREEISKRQVRVRESPTSEQVAKLHAAEARMKELKANMAVLGKE-AAAALASVDAQQQ 223
           E  R EI K    VRES   ++ A+  AAE +  ELKA  A   K+    AL+    Q +
Sbjct: 509 ELARYEIDKYDAAVRESAYLKECAEREAAERKETELKAIRAAXEKDKLEGALSGSVLQYR 330

Query: 224 RLTFQRLVA 232
           + T+  +V+
Sbjct: 329 KFTWDEIVS 303


>TC8858 homologue to UP|Q84N37 (Q84N37) Potyvirus VPg interacting protein
           (Fragment), partial (65%)
          Length = 1272

 Score = 31.6 bits (70), Expect = 0.19
 Identities = 25/83 (30%), Positives = 41/83 (49%), Gaps = 2/83 (2%)
 Frame = +1

Query: 162 QEAETLREEISKRQVRVRESPTSEQVAKLHAAEARMKELKANMAVLGKE--AAAALASVD 219
           +EA  L+ E  K+++++ E    E++ +L  AEA M +LKAN A    E     ALA  D
Sbjct: 607 REAGELKTERQKKKLQIEEL---ERIVRLKNAEADMFQLKANEAKREAERLQRIALAKSD 777

Query: 220 AQQQRLTFQRLVAMMVSDRQKKE 242
             ++  T   L   +     +K+
Sbjct: 778 KSEEEYTSNYLKQKLSEAEAEKQ 846


>TC9614 similar to UP|O22799 (O22799) At2g33490 protein, partial (12%)
          Length = 673

 Score = 28.1 bits (61), Expect = 2.1
 Identities = 21/78 (26%), Positives = 36/78 (45%), Gaps = 3/78 (3%)
 Frame = +2

Query: 49  QLEKLYKATR---TMRDFQKEIVKAAETFTAIGYKHIETGTKLSEDCCKYGAENNIDNIL 105
           QLE+L +ATR    MRD    ++ AA    +  Y+  E+   +     +  A N+ +   
Sbjct: 329 QLEELAQATRDMQDMRDCYDTLLSAAAATASSAYEFAESLRDMGSCLLEKTALNDHEEET 508

Query: 106 AKAASVLGDARKHVEKEH 123
            K   +LG  +  ++K H
Sbjct: 509 GKVLLMLGKIQFKLQKTH 562


>TC9443 similar to UP|Q84N37 (Q84N37) Potyvirus VPg interacting protein
           (Fragment), partial (41%)
          Length = 1025

 Score = 27.7 bits (60), Expect = 2.7
 Identities = 22/67 (32%), Positives = 33/67 (48%), Gaps = 2/67 (2%)
 Frame = +1

Query: 162 QEAETLREEISKRQVRVRESPTSEQVAKLHAAEARMKELKANMAVLGKE--AAAALASVD 219
           +E   L+ E  K++ +V E    E++ +L  AEA M +LKAN A    E     A A  D
Sbjct: 232 REVAELKMERQKKKSQVEEL---EKIVRLKNAEADMFQLKANEAKREAERLQRIAFAKPD 402

Query: 220 AQQQRLT 226
             ++  T
Sbjct: 403 KSEEEFT 423


>TC16726 similar to UP|GS11_ARATH (Q9LMP7) Golgi SNARE 11 protein (AtGOS11)
           (Golgi SNAP receptor complex member 1-1), partial (80%)
          Length = 655

 Score = 27.7 bits (60), Expect = 2.7
 Identities = 20/60 (33%), Positives = 29/60 (48%)
 Frame = +2

Query: 2   DALRKQASKLREQVSKQQQAVIKQFSTSGYESSDVVVIDEGEMQIHQQLEKLYKATRTMR 61
           DALRKQA  L  Q+ ++  +  K  S S    +D    D  E  I Q L++L +    M+
Sbjct: 140 DALRKQARNLEAQLDERMSSFRKLVSASVSAKTDAAENDL-ESWIEQLLKQLQQVNSQMQ 316


>BP045352 
          Length = 434

 Score = 27.7 bits (60), Expect = 2.7
 Identities = 12/39 (30%), Positives = 21/39 (53%)
 Frame = -3

Query: 90  EDCCKYGAENNIDNILAKAASVLGDARKHVEKEHEELNR 128
           E   +  AE N +    ++   + + RKH+E+ HEEL +
Sbjct: 291 EPAARLKAEENANLARVRSDKEIRELRKHLERAHEELRQ 175


>BP051546 
          Length = 574

 Score = 26.9 bits (58), Expect = 4.7
 Identities = 11/32 (34%), Positives = 17/32 (52%)
 Frame = -2

Query: 92  CCKYGAENNIDNILAKAASVLGDARKHVEKEH 123
           CC  G +NNID+  AK        + H +++H
Sbjct: 510 CCAPGCKNNIDHPRAKPLKDFRTLQTHYKRKH 415


>TC14602 UP|Q8GS56 (Q8GS56) Phosphoenolpyruvate carboxylase kinase, complete
          Length = 1256

 Score = 26.6 bits (57), Expect = 6.1
 Identities = 10/22 (45%), Positives = 13/22 (58%)
 Frame = +2

Query: 255 GKTMYFLAEATHPFYGDSEKEL 276
           G  +Y +   T PFYGDS  E+
Sbjct: 629 GVILYIMLSGTPPFYGDSAAEI 694


>TC13257 
          Length = 544

 Score = 26.2 bits (56), Expect = 8.0
 Identities = 13/27 (48%), Positives = 16/27 (59%)
 Frame = +1

Query: 241 KESAPPVVVSENGSGKTMYFLAEATHP 267
           K   PP   S NG GKT+  +A+A HP
Sbjct: 55  KLKVPP---SSNGDGKTVAVVADAFHP 126


  Database: LJGI
    Posted date:  Jul 30, 2004 11:16 AM
  Number of letters in database: 14,692,800
  Number of sequences in database:  28,460
  
Lambda     K      H
   0.313    0.127    0.343 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,778,160
Number of Sequences: 28460
Number of extensions: 37065
Number of successful extensions: 168
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 168
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 168
length of query: 327
length of database: 4,897,600
effective HSP length: 91
effective length of query: 236
effective length of database: 2,307,740
effective search space: 544626640
effective search space used: 544626640
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 55 (25.8 bits)


Lotus: description of TM0046a.5