BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0044a.5
(578 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP063694 62 2e-10
AV766088 57 6e-09
BP033602 50 1e-06
TC17853 similar to UP|Q42412 (Q42412) RNA-binding protein RZ-1, ... 40 0.001
BP063902 37 0.007
BI418821 37 0.011
AV420911 36 0.020
TC18003 similar to PIR|T05112|T05112 splicing factor 9G8-like SR... 36 0.020
TC11095 similar to PIR|T05112|T05112 splicing factor 9G8-like SR... 35 0.026
AV421607 34 0.074
BP058111 34 0.074
BP030995 32 0.37
TC13322 similar to UP|Q9ZS13 (Q9ZS13) RNA helicase (Fragment), p... 30 0.82
BP054351 30 1.1
BP065763 29 1.8
AV773507 29 2.4
BP078157 28 3.1
TC10011 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, par... 28 3.1
TC9521 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, part... 28 3.1
>BP063694
Length = 511
Score = 62.4 bits (150), Expect = 2e-10
Identities = 41/123 (33%), Positives = 63/123 (50%), Gaps = 4/123 (3%)
Frame = -2
Query: 426 SVSLWHSRLAHIGISTMNRLIKSKL--ISCNIHEFEKCEIC--VKSKMIKKHFKSVERKF 481
S LWHSRL H+ + +L K +S + + C C KSK + H + +R
Sbjct: 369 SFELWHSRLGHVNFDIIKQLHKHGCLDVSSILPKPICCTSCQMAKSKRLVFHDNN-KRAS 193
Query: 482 NLLDLVHSDLCEFNGMLTRGGNRYFITFIDDCSRYTHVYLLKHKDDAFNAFKSYKAEVEN 541
+LDL+H DL + + + G YF+ F+DD SR+T Y LK K D + +K +EN
Sbjct: 192 AVLDLIHCDLRGPSPVASIDGFSYFVIFVDDFSRFTWFYPLKRKSDFSDVLLRFKVFMEN 13
Query: 542 QLN 544
+ +
Sbjct: 12 RFS 4
>AV766088
Length = 501
Score = 57.4 bits (137), Expect = 6e-09
Identities = 34/104 (32%), Positives = 55/104 (52%), Gaps = 2/104 (1%)
Frame = -3
Query: 428 SLWHSRLAHIGISTMNRLIKSKLISCNIHEFEK-CEICVKSKMIKKHFKSVERKFNL-LD 485
SLWHSRL H S + L +K IS + + CE CV K ++ F S + D
Sbjct: 310 SLWHSRLGHPSSSALRYLRSNKFISYELLNYSPVCESCVFGKHVRLPFVSSNNVTVMPFD 131
Query: 486 LVHSDLCEFNGMLTRGGNRYFITFIDDCSRYTHVYLLKHKDDAF 529
++HSDL + +L+ G+R+++ F+DD + + + L +K F
Sbjct: 130 ILHSDLWT-SPVLSSAGHRFYVLFLDDFTDFLWTFPLSNKSQVF 2
>BP033602
Length = 533
Score = 49.7 bits (117), Expect = 1e-06
Identities = 31/92 (33%), Positives = 45/92 (48%), Gaps = 4/92 (4%)
Frame = +3
Query: 425 DSVSLWHSRLAHIGISTMNRLIKSKLISCNIHEFEKCEICVKSKMIKKHFKS----VERK 480
D + LWH+RL H + L + N E CE CV +K + + S R
Sbjct: 264 DQIMLWHNRLGHPNFQYLRHLFPDLFKNVNCSSLE-CESCVLAKNQRAPYYSQPYHASRP 440
Query: 481 FNLLDLVHSDLCEFNGMLTRGGNRYFITFIDD 512
F L +HSD+ + + T+ G R+F+TFIDD
Sbjct: 441 FYL---IHSDVWGPSKITTQFGKRWFVTFIDD 527
>TC17853 similar to UP|Q42412 (Q42412) RNA-binding protein RZ-1, partial
(58%)
Length = 881
Score = 40.0 bits (92), Expect = 0.001
Identities = 20/65 (30%), Positives = 34/65 (51%), Gaps = 1/65 (1%)
Frame = +2
Query: 198 DKAESSGFSKANTVTTKGKKKYDGKQQHLGPKKEHNKFKNNNGTKGPKGG-CYVCGKPDH 256
DKA+ G S+ +Y + + + + ++ + G++G GG C+ CGKP H
Sbjct: 278 DKAQPQGSSRDRD----DGDRYRERGRDRDDRGDRDRSRGYGGSRGSNGGECFKCGKPGH 445
Query: 257 FARDC 261
FAR+C
Sbjct: 446 FAREC 460
>BP063902
Length = 494
Score = 37.4 bits (85), Expect = 0.007
Identities = 19/50 (38%), Positives = 25/50 (50%), Gaps = 1/50 (2%)
Frame = -3
Query: 428 SLWHSRLAHIGISTMNRLIKSKLISCN-IHEFEKCEICVKSKMIKKHFKS 476
S+WH RL H S +N L +KLI C C+ CV K ++ F S
Sbjct: 186 SVWHHRLGHPASSALNHLRNNKLIFCEPSRSSSVCDSCVLGKHVRLPFSS 37
>BI418821
Length = 614
Score = 36.6 bits (83), Expect = 0.011
Identities = 14/29 (48%), Positives = 17/29 (58%)
Frame = +2
Query: 236 KNNNGTKGPKGGCYVCGKPDHFARDCRQN 264
+N G G GGCY CG H ARDC ++
Sbjct: 356 RNGGGGGGGGGGCYNCGDTGHLARDCHRS 442
Score = 35.8 bits (81), Expect = 0.020
Identities = 14/28 (50%), Positives = 16/28 (57%)
Frame = +2
Query: 237 NNNGTKGPKGGCYVCGKPDHFARDCRQN 264
NNNG G CY CG H ARDC ++
Sbjct: 443 NNNGGGGGGAACYNCGDAGHLARDCNRS 526
Score = 35.8 bits (81), Expect = 0.020
Identities = 13/29 (44%), Positives = 18/29 (61%)
Frame = +2
Query: 236 KNNNGTKGPKGGCYVCGKPDHFARDCRQN 264
++NN + G GCY CG H ARDC ++
Sbjct: 521 RSNNNSGGGGAGCYNCGDTGHLARDCNRS 607
>AV420911
Length = 418
Score = 35.8 bits (81), Expect = 0.020
Identities = 16/36 (44%), Positives = 20/36 (55%), Gaps = 10/36 (27%)
Frame = +2
Query: 237 NNNGTKGPKGG----------CYVCGKPDHFARDCR 262
N+ G G +GG CY CG+P HFAR+CR
Sbjct: 311 NSKGGGGGRGGGRGRGGEDLKCYECGEPGHFARECR 418
>TC18003 similar to PIR|T05112|T05112 splicing factor 9G8-like SR protein
RSZp22 [validated] - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (42%)
Length = 587
Score = 35.8 bits (81), Expect = 0.020
Identities = 16/36 (44%), Positives = 20/36 (55%), Gaps = 10/36 (27%)
Frame = +1
Query: 237 NNNGTKGPKGG----------CYVCGKPDHFARDCR 262
N+ G G +GG CY CG+P HFAR+CR
Sbjct: 103 NSKGGGGGRGGGRGRGGEDLKCYECGEPGHFARECR 210
>TC11095 similar to PIR|T05112|T05112 splicing factor 9G8-like SR protein
RSZp22 [validated] - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (89%)
Length = 912
Score = 35.4 bits (80), Expect = 0.026
Identities = 17/44 (38%), Positives = 20/44 (44%), Gaps = 13/44 (29%)
Frame = +1
Query: 232 HNKFKNNNGTKGPKGG-------------CYVCGKPDHFARDCR 262
HN G G +GG CY CG+P HFAR+CR
Sbjct: 292 HNSRGGGGGGGGGRGGGRSGGGGGGSDMKCYECGEPGHFARECR 423
>AV421607
Length = 245
Score = 33.9 bits (76), Expect = 0.074
Identities = 12/29 (41%), Positives = 16/29 (54%)
Frame = +3
Query: 243 GPKGGCYVCGKPDHFARDCRQNKTKKEVN 271
G GCY CG+P H++RDC + N
Sbjct: 39 GTATGCYKCGRPGHWSRDCPSSAPNSNPN 125
>BP058111
Length = 570
Score = 33.9 bits (76), Expect = 0.074
Identities = 25/81 (30%), Positives = 37/81 (44%), Gaps = 2/81 (2%)
Frame = -1
Query: 411 DNIINKVSNSAYMIDSVSLWHSRLAHIGISTMNRLIKS-KLISCNIHEFEKCEICVKSKM 469
DNI +VS+ + +LWH RL H + L K+ I+C C+ C +K
Sbjct: 240 DNIKTQVSHFNFCQFDCNLWHLRLGHTSSKKLAILQKNFPFITCK-KITSPCDTCHMAKQ 64
Query: 470 IKKHF-KSVERKFNLLDLVHS 489
K F SV + DL+H+
Sbjct: 63 KKLSFPNSVTLSSEIFDLIHT 1
>BP030995
Length = 458
Score = 31.6 bits (70), Expect = 0.37
Identities = 38/149 (25%), Positives = 56/149 (37%), Gaps = 45/149 (30%)
Frame = -3
Query: 351 VTLVNVLHVPEMSRDLVSGDLLGKPG------------IKSVYESEKLI--LTRNGVFVG 396
+ L N+LHVP + ++L S K +KS E L+ RNG
Sbjct: 426 LVLNNLLHVPGVCKNLSSVSXFCKQNNVFFEFHSDSCLVKSQETEETLLQGTLRNG---- 259
Query: 397 KGYSAEGMIKLCPIDNIINKV--------SNSAYM-----------------------ID 425
LC DN+++KV SNSA + ID
Sbjct: 258 ----------LCAFDNLLHKVNLHSASTLSNSALLSSSSPASALSVSVFSSNKCAQADID 109
Query: 426 SVSLWHSRLAHIGISTMNRLIKSKLISCN 454
S LWHSRL H ++ ++K ++ C+
Sbjct: 108 S-RLWHSRLGHASQKIVSHVLKHVILLCH 25
>TC13322 similar to UP|Q9ZS13 (Q9ZS13) RNA helicase (Fragment), partial (7%)
Length = 528
Score = 30.4 bits (67), Expect = 0.82
Identities = 26/106 (24%), Positives = 44/106 (40%)
Frame = +3
Query: 185 QKHLRIEEESKVRDKAESSGFSKANTVTTKGKKKYDGKQQHLGPKKEHNKFKNNNGTKGP 244
+KH + V D+A +S ++ K K + PK++ + N NGT+
Sbjct: 60 RKHDAVAASEPVADEAPNSPELQSEKKKKKKNKDKHQNGEKTSPKRKLEELDNQNGTESE 239
Query: 245 KGGCYVCGKPDHFARDCRQNKTKKEVNAVQVDDEIIATVSEVMAVK 290
K K D+ + + + KE N DE +A +EV+ K
Sbjct: 240 KKKKKKKEKHDNKSEE--NAEETKEANGNGNADETVADGAEVVTGK 371
>BP054351
Length = 431
Score = 30.0 bits (66), Expect = 1.1
Identities = 14/41 (34%), Positives = 18/41 (43%)
Frame = -1
Query: 425 DSVSLWHSRLAHIGISTMNRLIKSKLISCNIHEFEKCEICV 465
D + LWH+RL H + L + N E CE CV
Sbjct: 122 DQIMLWHNRLGHPNFQYLRHLFPDLFKNVNCSSLE-CESCV 3
>BP065763
Length = 537
Score = 29.3 bits (64), Expect = 1.8
Identities = 20/86 (23%), Positives = 34/86 (39%)
Frame = -1
Query: 191 EEESKVRDKAESSGFSKANTVTTKGKKKYDGKQQHLGPKKEHNKFKNNNGTKGPKGGCYV 250
+EE +++ + + S + + K K K+Q K+ K G + CY
Sbjct: 537 QEEGRLKKEKDHSIHLTTHDGASXSKAKPGRKEQ-----KKDQALKVKEGRIHKEHVCYF 373
Query: 251 CGKPDHFARDCRQNKTKKEVNAVQVD 276
C K HF + C + K E + D
Sbjct: 372 CKKAGHFKKYCPKRKAWFEKKGIPFD 295
>AV773507
Length = 496
Score = 28.9 bits (63), Expect = 2.4
Identities = 10/22 (45%), Positives = 14/22 (63%)
Frame = +2
Query: 240 GTKGPKGGCYVCGKPDHFARDC 261
G + + C+ CG+P H ARDC
Sbjct: 326 GDRVGQDDCFECGRPGHRARDC 391
>BP078157
Length = 395
Score = 28.5 bits (62), Expect = 3.1
Identities = 16/51 (31%), Positives = 23/51 (44%), Gaps = 3/51 (5%)
Frame = -2
Query: 429 LWHSRLAHIGISTMNRLIKSKLISCNI---HEFEKCEICVKSKMIKKHFKS 476
LWHSRL H+ + + S L+ + C +C SKM + F S
Sbjct: 148 LWHSRLGHLSDKVLK--VVSNLVPFTVLPDFHSHNCNVCPLSKMRRLSFVS 2
>TC10011 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, partial (62%)
Length = 684
Score = 28.5 bits (62), Expect = 3.1
Identities = 12/31 (38%), Positives = 17/31 (54%), Gaps = 3/31 (9%)
Frame = +2
Query: 242 KGP---KGGCYVCGKPDHFARDCRQNKTKKE 269
+GP G C+ CG H+ARDC+ K +
Sbjct: 344 RGPPPGSGRCFNCGLDGHWARDCKAGDWKNK 436
Score = 28.1 bits (61), Expect = 4.1
Identities = 16/61 (26%), Positives = 27/61 (44%), Gaps = 5/61 (8%)
Frame = +2
Query: 214 KGKKKYDGKQQHLGPKKEHNKFKNNNGTKGPKGG-----CYVCGKPDHFARDCRQNKTKK 268
+G ++Y G+ G + N + + + K G CY CG H R+C+ + K
Sbjct: 320 RGNREYLGRGPPPGSGRCFNCGLDGHWARDCKAGDWKNKCYRCGDRGHVERNCKNSPKKN 499
Query: 269 E 269
E
Sbjct: 500 E 502
>TC9521 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, partial (56%)
Length = 598
Score = 28.5 bits (62), Expect = 3.1
Identities = 12/43 (27%), Positives = 20/43 (45%)
Frame = +1
Query: 227 GPKKEHNKFKNNNGTKGPKGGCYVCGKPDHFARDCRQNKTKKE 269
G ++ ++ G G C+ CG H+ARDC+ K +
Sbjct: 328 GGGRDRDREYMGRGPPPGSGRCFNCGIDGHWARDCKAGDWKNK 456
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.318 0.135 0.397
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,133,906
Number of Sequences: 28460
Number of extensions: 114048
Number of successful extensions: 618
Number of sequences better than 10.0: 45
Number of HSP's better than 10.0 without gapping: 609
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 616
length of query: 578
length of database: 4,897,600
effective HSP length: 95
effective length of query: 483
effective length of database: 2,193,900
effective search space: 1059653700
effective search space used: 1059653700
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 58 (26.9 bits)
Lotus: description of TM0044a.5