BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0039.13
(673 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP063300 84 7e-17
AV423371 37 0.008
AI967308 37 0.008
TC13195 33 0.20
TC10133 similar to UP|Q9LSS5 (Q9LSS5) Myosin heavy chain-like, p... 32 0.43
TC15757 weakly similar to GB|AAP37804.1|30725564|BT008445 At2g15... 31 0.57
TC15534 weakly similar to GB|AAO63420.1|28950993|BT005356 At5g28... 31 0.74
TC15394 similar to UP|Q9FFQ8 (Q9FFQ8) Emb|CAB62360.1, partial (16%) 30 1.7
TC18612 30 1.7
TC19034 30 1.7
TC18231 similar to UP|Q9LNG5 (Q9LNG5) F21D18.16 (Serine/threoni... 29 2.2
AV412834 29 2.2
TC14103 similar to UP|Q84XC6 (Q84XC6) Plasma intrinsic protein 2... 29 2.8
TC11509 similar to UP|Q80TY1 (Q80TY1) MKIAA0553 protein (Fragmen... 29 2.8
BI420177 29 2.8
BP071102 28 6.3
TC15746 27 8.2
TC12621 27 8.2
>BP063300
Length = 507
Score = 84.0 bits (206), Expect = 7e-17
Identities = 43/91 (47%), Positives = 57/91 (62%), Gaps = 4/91 (4%)
Frame = +1
Query: 5 EKKKGKAVGGGPKPKMSK----DAPKRKKIDREKWPHLTGDRAHLDDRCREILGIESCYA 60
+K+KGK V P ++ APK KKIDREK+PHL GD D CR IL + S Y
Sbjct: 235 QKRKGKTVDEAPDGALTSRPRSGAPKSKKIDREKFPHLKGDEXVYDKWCRTILDLPSEYX 414
Query: 61 NEDEGHFRRQYYLSEKQIGSGHDVFAPGKKE 91
+E+E +F Q ++S+ QIG GH V++P KKE
Sbjct: 415 HENEDYFWSQRFMSQTQIGPGHMVYSPSKKE 507
>AV423371
Length = 437
Score = 37.4 bits (85), Expect = 0.008
Identities = 43/139 (30%), Positives = 66/139 (46%), Gaps = 10/139 (7%)
Frame = +3
Query: 476 RASLKTASAVRYLQGSTIPELEELREKTEKDDQLISSMSKELEEGCSAIEELSKALDNMK 535
++SL + + T+ LE +KT D ++ + +KE +G I EL K L K
Sbjct: 3 KSSLASIRQREGMASITVSSLEAELDKTRSD--IVLAQAKE-NDGREMIIELPKKL---K 164
Query: 536 LENEQLKKKVQDGDAVREDLEGRLKAAEGKAETGAARL--------KEAEAAWLKEREQS 587
E+ + +A RE+L R+K +A+ GA+ + KE EAA ER
Sbjct: 165 EAAEEANQANLHAEAAREELR-RVKEEAEQAKAGASTMQSKLLAAQKEIEAARASERLAL 341
Query: 588 SAVK--KELENRVSSLEAD 604
SAVK +E E+ S+ E D
Sbjct: 342 SAVKALQESESARSNNEVD 398
>AI967308
Length = 443
Score = 37.4 bits (85), Expect = 0.008
Identities = 26/89 (29%), Positives = 47/89 (52%), Gaps = 4/89 (4%)
Frame = +1
Query: 494 PELEELREKTEKDDQLISSMSKELEEGCSAIEELSKALDNMKLENEQLKKKVQDGDAVRE 553
PE+ + E T K+D+ I+ + LEE + +E +SK +N+K + + KV + + E
Sbjct: 178 PEIPCVDEMTLKNDE-IALLKCNLEEKVTELESMSKENENLKQQLNEAVSKVSEAETKEE 354
Query: 554 DLEGRLK----AAEGKAETGAARLKEAEA 578
++ +LK E ++ A +LK EA
Sbjct: 355 GMKLQLKELGEELEAASKLNAEKLKCVEA 441
>TC13195
Length = 482
Score = 32.7 bits (73), Expect = 0.20
Identities = 22/74 (29%), Positives = 35/74 (46%)
Frame = +1
Query: 289 CSYSVRRNAKLLRGMADIDRTLLDALRVEDGQSTDEEVDQETDVAQGEEGHAENQPQPDP 348
C+ SVR+ L G ++ R+LL + S D + E + G E E++P+P P
Sbjct: 49 CTDSVRK----LNGSRNVVRSLLQNSMADVRASQDSTLSSEINKNGGSE--PESEPEPVP 210
Query: 349 EDRGGAEVGGTSGS 362
D G +SG+
Sbjct: 211 SDNESVTSGSSSGA 252
>TC10133 similar to UP|Q9LSS5 (Q9LSS5) Myosin heavy chain-like, partial (34%)
Length = 1827
Score = 31.6 bits (70), Expect = 0.43
Identities = 25/126 (19%), Positives = 62/126 (48%), Gaps = 1/126 (0%)
Frame = +1
Query: 498 ELREKTEKDDQLISSMSKELEEGCSAIEELSKALDNMKLENEQLKKKVQDGDAVR-EDLE 556
ELR + ++ + ++++ ++ + + ++ +S+ + +KLE + K ++ +A + + E
Sbjct: 1102 ELRNEIKRLEHCVAALKADMMDKETTLQSISEENEMLKLE----RNKSEEIEAAKAAEHE 1269
Query: 557 GRLKAAEGKAETGAARLKEAEAAWLKEREQSSAVKKELENRVSSLEADKKKLAATVSKIN 616
+K E ++ K A E Q+S + E E R +++D+ + AA +
Sbjct: 1270 ALMKLGIAMQEVESSSRKAARVTEQLEAAQASNSEMEAELRRMKVQSDQWRKAAEAAAAM 1449
Query: 617 KASFNN 622
++ NN
Sbjct: 1450 LSAGNN 1467
>TC15757 weakly similar to GB|AAP37804.1|30725564|BT008445 At2g15890
{Arabidopsis thaliana;}, partial (67%)
Length = 913
Score = 31.2 bits (69), Expect = 0.57
Identities = 28/78 (35%), Positives = 38/78 (47%), Gaps = 6/78 (7%)
Frame = +3
Query: 493 IPELEELR----EKTEKDDQLISSMSKELEEGCSAIE-ELSKALDNMKLENEQLKKKVQD 547
IP+LE E+ KD LI KEL + CSA+E E S + E + L+K+
Sbjct: 147 IPKLEPFSRSKSERAAKDPPLIEKSEKELLDYCSAVEGEESYSCWQAYFELKDLEKESPK 326
Query: 548 GDAVREDLE-GRLKAAEG 564
D R LE G +K+ G
Sbjct: 327 SDIERLILETGGVKSLVG 380
>TC15534 weakly similar to GB|AAO63420.1|28950993|BT005356 At5g28040
{Arabidopsis thaliana;}, partial (8%)
Length = 937
Score = 30.8 bits (68), Expect = 0.74
Identities = 17/55 (30%), Positives = 24/55 (42%)
Frame = -3
Query: 375 DPVQGKGRSVSEEKDTRGPASKKRRVEDHQDHADGEVQGSEPERAEPVTVVPPSP 429
DP S SEE+D + P S+ + ++ E +GS E E T P P
Sbjct: 854 DPPTASSSSESEEEDDQ-PLSQVKHAAAEEEELSSEEEGSSEEEEEDETTAPAPP 693
>TC15394 similar to UP|Q9FFQ8 (Q9FFQ8) Emb|CAB62360.1, partial (16%)
Length = 1093
Score = 29.6 bits (65), Expect = 1.7
Identities = 23/112 (20%), Positives = 50/112 (44%), Gaps = 13/112 (11%)
Frame = +2
Query: 317 EDGQSTDEEVDQETDVAQGEEGHAENQPQPDPE---DRGGAEVGGTSGSAKNVSLEADHG 373
E+ + +E D + ++ EE ++ Q + + + G++ G + SAK +++
Sbjct: 44 ENVAAPHKESDDDESKSEEEEDKSKAQKKTSKKIVKEGSGSKAGERTKSAKKTPVKSSKN 223
Query: 374 SD--PVQGKGRSVSEEKDT--------RGPASKKRRVEDHQDHADGEVQGSE 415
D P + + + E D+ + PASKKR+ E+ + G+ +
Sbjct: 224 VDKTPKKSTPKQTAAEHDSASASLSKSKQPASKKRKTENEKPDTKGKASSKK 379
>TC18612
Length = 881
Score = 29.6 bits (65), Expect = 1.7
Identities = 12/43 (27%), Positives = 26/43 (59%)
Frame = +3
Query: 505 KDDQLISSMSKELEEGCSAIEELSKALDNMKLENEQLKKKVQD 547
+DD+ ++S+ + E+ AIE+ A + K E+ ++K+Q+
Sbjct: 261 QDDEYLASLQADREKELKAIEKAEAAREEEKRREEEARRKLQE 389
>TC19034
Length = 843
Score = 29.6 bits (65), Expect = 1.7
Identities = 33/132 (25%), Positives = 60/132 (45%), Gaps = 9/132 (6%)
Frame = +1
Query: 494 PELEELREKTEKDDQLISSMSKELEEGCSAIEELSK-----ALDNMKLENE--QLKKKVQ 546
PE+ KDD + ++L+ G E+S D+ K + + + K
Sbjct: 379 PEVSPNPPSPGKDDSKKDTDQEDLKTGTENPPEVSPNPPSPGKDDSKKDTDLPEESDKQV 558
Query: 547 DGDAVREDLEGRLKAAEGKAETGAARLKEAEAAWLKEREQSSAVK--KELENRVSSLEAD 604
G + ++ +LK + ++ AA+LK + L++ Q + K KE++N SS +AD
Sbjct: 559 SGKSTPRTMKAKLKIVK---KSLAAKLKAKKNNGLQKIRQKTINKNKKEVDNAESSHDAD 729
Query: 605 KKKLAATVSKIN 616
KK + S+ N
Sbjct: 730 KKHIPDNNSQKN 765
>TC18231 similar to UP|Q9LNG5 (Q9LNG5) F21D18.16 (Serine/threonine protein
phosphatase) , partial (7%)
Length = 567
Score = 29.3 bits (64), Expect = 2.2
Identities = 25/81 (30%), Positives = 33/81 (39%), Gaps = 4/81 (4%)
Frame = +1
Query: 365 NVSLEAD--HGS--DPVQGKGRSVSEEKDTRGPASKKRRVEDHQDHADGEVQGSEPERAE 420
+++L AD HGS DP G + SEE++ Q S P +
Sbjct: 16 SMTLHADSHHGSPPDPATSNGAATSEEENAAQ-------------------QSSPPPSSC 138
Query: 421 PVTVVPPSPERLSIWGPRAGN 441
P+ PP RL IW P GN
Sbjct: 139 PI---PPEELRLPIWWPEDGN 192
>AV412834
Length = 296
Score = 29.3 bits (64), Expect = 2.2
Identities = 10/16 (62%), Positives = 10/16 (62%)
Frame = +3
Query: 205 RVAPTSPPPFWWFEQG 220
R P SP PFWWF G
Sbjct: 225 RPPPDSPRPFWWFSSG 272
>TC14103 similar to UP|Q84XC6 (Q84XC6) Plasma intrinsic protein 2,2, partial
(94%)
Length = 1199
Score = 28.9 bits (63), Expect = 2.8
Identities = 18/68 (26%), Positives = 28/68 (40%), Gaps = 1/68 (1%)
Frame = -1
Query: 336 EEGHAENQPQPDPEDRGGAEVGGTSG-SAKNVSLEADHGSDPVQGKGRSVSEEKDTRGPA 394
+E HA P + A VGG SG V+ + + G + G + +E GP
Sbjct: 368 DENHAAEGPSDAEDANAAARVGGVSGVGLAAVADDGEDGDVEEEQGGDELGDEGSVEGPL 189
Query: 395 SKKRRVED 402
+ VE+
Sbjct: 188 GELGGVEE 165
>TC11509 similar to UP|Q80TY1 (Q80TY1) MKIAA0553 protein (Fragment), partial
(3%)
Length = 624
Score = 28.9 bits (63), Expect = 2.8
Identities = 26/129 (20%), Positives = 46/129 (35%), Gaps = 5/129 (3%)
Frame = -1
Query: 282 PLRCYDLCSYSVRRNAKLLRGMADIDRTLLDALRVEDGQSTDEEVDQETDVAQGEEGHAE 341
P C+ L + L++G+AD+ D R + QS D+E + A+ G +
Sbjct: 558 PSHCFSLSEFPQDIMEGLIKGLADVILGANDQDRDQHHQSHDDEERSRSSWAEVVSGDQD 379
Query: 342 NQPQPDPEDRGGAEVGGTSGSAKNVSLEADHGSDPVQGK-----GRSVSEEKDTRGPASK 396
Q + G+ ++ + E D K R E + P
Sbjct: 378 QDQQDQTQSHQGSGSRFSNRPHREKEEEESWRDDSYSHKEKYRPQREECESESFNSPNRP 199
Query: 397 KRRVEDHQD 405
+ +ED+ D
Sbjct: 198 PKLIEDNND 172
>BI420177
Length = 444
Score = 28.9 bits (63), Expect = 2.8
Identities = 14/49 (28%), Positives = 22/49 (44%)
Frame = +3
Query: 337 EGHAENQPQPDPEDRGGAEVGGTSGSAKNVSLEADHGSDPVQGKGRSVS 385
E + + +PDP D +E + GS K+ E D P K + +S
Sbjct: 219 EPQNDGRSEPDPSDDLSSEEAASDGSKKSPKRELDEEQQPASKKQKPLS 365
>BP071102
Length = 450
Score = 27.7 bits (60), Expect = 6.3
Identities = 16/42 (38%), Positives = 18/42 (42%)
Frame = -2
Query: 16 PKPKMSKDAPKRKKIDREKWPHLTGDRAHLDDRCREILGIES 57
P P + R I R+ HL G AHLD R G ES
Sbjct: 218 PTPSFLRRLLHRDYIGRKNGKHLCGT*AHLDLRSSSFAGAES 93
>TC15746
Length = 619
Score = 27.3 bits (59), Expect = 8.2
Identities = 29/120 (24%), Positives = 50/120 (41%), Gaps = 2/120 (1%)
Frame = +2
Query: 524 IEELSKALDNMKLENEQLKKKVQDGDAVREDLEGRLKAAEGKAETGAARLKEAEAAWLKE 583
+ ++ + +MK EN ++ + + E+L+ +L + K L+EAEA +
Sbjct: 26 VTRYTENVGDMKSENGDVQLNLAEDS---EELKSKLNIMDSK-------LREAEATIMML 175
Query: 584 REQSSAVKKELENRVSSLEAD--KKKLAATVSKINKASFNNAVSQLAVVNPGLETAPVGY 641
E E R+++ E D K++L KIN L V L + VGY
Sbjct: 176 NE---------ERRMNTKEKDFFKRELEVLKKKINTKGVQTGFPLLFVCMVALVSVAVGY 328
>TC12621
Length = 897
Score = 27.3 bits (59), Expect = 8.2
Identities = 27/94 (28%), Positives = 38/94 (39%), Gaps = 8/94 (8%)
Frame = +1
Query: 341 ENQPQPDPEDRGGAEV--------GGTSGSAKNVSLEADHGSDPVQGKGRSVSEEKDTRG 392
E+ P +P++ G V S SA + SL D S +E +D G
Sbjct: 376 EDSPVENPDNVGSTSVPVQSVPEDSSESASAFDGSLYKDINSYQSHRHPFDNNEAED--G 549
Query: 393 PASKKRRVEDHQDHADGEVQGSEPERAEPVTVVP 426
+S +E H D QG+EPE P V+P
Sbjct: 550 TSSVAANLEQLNLHNDD--QGTEPEEENPSVVIP 645
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.314 0.134 0.392
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,332,284
Number of Sequences: 28460
Number of extensions: 136923
Number of successful extensions: 654
Number of sequences better than 10.0: 37
Number of HSP's better than 10.0 without gapping: 650
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 651
length of query: 673
length of database: 4,897,600
effective HSP length: 96
effective length of query: 577
effective length of database: 2,165,440
effective search space: 1249458880
effective search space used: 1249458880
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 58 (26.9 bits)
Lotus: description of TM0039.13