BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0034b.2
(494 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC14645 similar to UP|Q41122 (Q41122) Proline-rich protein precu... 38 0.003
AV780231 38 0.004
TC13802 similar to UP|Q9M1T1 (Q9M1T1) Sugar-phosphate isomerase-... 34 0.048
TC17919 similar to UP|KPYA_RICCO (Q43117) Pyruvate kinase isozym... 34 0.048
AW720177 33 0.081
TC12700 similar to UP|P93390 (P93390) Phosphate/phosphoenolpyruv... 33 0.11
AU089273 32 0.24
TC7849 similar to GB|AAN18126.1|23308313|BT000557 At2g10940/F15K... 32 0.31
AV406930 31 0.40
BI418438 30 0.69
TC18336 similar to UP|Q84KA9 (Q84KA9) RING/C3HC4/PHD zinc finger... 30 0.69
TC9284 weakly similar to UP|Q9LPY0 (Q9LPY0) T23J18.22, partial (... 30 1.2
AV766417 29 1.5
TC14545 similar to UP|Q9FT78 (Q9FT78) P23 co-chaperone, partial ... 29 1.5
BP038767 29 1.5
TC9777 similar to UP|SOX_ARATH (Q9SJA7) Potential sarcosine oxid... 29 1.5
TC12321 similar to UP|Q15415 (Q15415) YRRM2, partial (5%) 29 1.5
AV407514 29 2.0
TC13364 similar to UP|Q9LUM3 (Q9LUM3) Subtilisin proteinase-like... 29 2.0
BP066856 28 3.4
>TC14645 similar to UP|Q41122 (Q41122) Proline-rich protein precursor,
partial (52%)
Length = 1060
Score = 38.1 bits (87), Expect = 0.003
Identities = 33/126 (26%), Positives = 54/126 (42%)
Frame = +2
Query: 79 FHNRPIVRSNSPLHATHLPKTIMTSNLPHASNTRHPSSFSPSPIKTLLPHSHPFLRPLFT 138
+HN P+ + P H+ P ++ PH + HP + +P+PIK P HP
Sbjct: 212 YHNHPVAPVHPPTHSPIHPP----AHAPHHHHHHHPPAPAPAPIK---PPVHP------P 352
Query: 139 SLIAYQTPSAHFRSGAVAYHPFRYPLFTSYSGESHCPYLCITHILIFSFLSLHCLLKMAS 198
+ + + P+ H + P P Y+ + P L TH+ S +L L K+ S
Sbjct: 353 TSVPVKPPTGHHHH---HHPPSPAPAPALYTTITLPPLLLFTHLFTPSLEAL-LLFKVLS 520
Query: 199 NPTSPN 204
P+ N
Sbjct: 521 LPSPAN 538
>AV780231
Length = 530
Score = 37.7 bits (86), Expect = 0.004
Identities = 17/17 (100%), Positives = 17/17 (100%)
Frame = -3
Query: 407 AKSSFARKYFRVAVHPA 423
AKSSFARKYFRVAVHPA
Sbjct: 528 AKSSFARKYFRVAVHPA 478
>TC13802 similar to UP|Q9M1T1 (Q9M1T1) Sugar-phosphate isomerase-like
protein, partial (41%)
Length = 522
Score = 34.3 bits (77), Expect = 0.048
Identities = 43/171 (25%), Positives = 66/171 (38%), Gaps = 2/171 (1%)
Frame = +2
Query: 80 HNRPIVRSNSPLHATHLPKTIMTSNLPH--ASNTRHPSSFSPSPIKTLLPHSHPFLRPLF 137
H + + N PLH+T S+ P+ S T P+S +P P + P S
Sbjct: 29 HFQSTILPNKPLHSTLTKPPSQISSNPNKTTSTTSSPTSTTPKPSPSPAPSS-------- 184
Query: 138 TSLIAYQTPSAHFRSGAVAYHPFRYPLFTSYSGESHCPYLCITHILIFSFLSLHCLLKMA 197
+ PS+ S + A P + P +S S + +T S
Sbjct: 185 ----TLRAPSSSPASASPASSPTKSPKPSSPSASAPPSSTPLTPSTATS----------E 322
Query: 198 SNPTSPNHSSSSSGSDPDATAAGGLRLEAPEIPPYRLKTYATLPLPVQIAP 248
S+PT+ + SSS+S + P + +A LE LK A+ P P P
Sbjct: 323 SSPTATSSSSSASPAPPTSFSALSRALE--------LKALASSPSPPSSPP 451
>TC17919 similar to UP|KPYA_RICCO (Q43117) Pyruvate kinase isozyme A,
chloroplast precursor , partial (11%)
Length = 566
Score = 34.3 bits (77), Expect = 0.048
Identities = 27/78 (34%), Positives = 33/78 (41%), Gaps = 7/78 (8%)
Frame = +1
Query: 76 HFPFHNRPI-------VRSNSPLHATHLPKTIMTSNLPHASNTRHPSSFSPSPIKTLLPH 128
H HN P + SP H T P T TS+LPH+S+ P++ SPS PH
Sbjct: 130 HIHIHNPPCQPLTPSTTFNTSPQHRTPPPST-PTSHLPHSSSPAPPATTSPS-FAPPPPH 303
Query: 129 SHPFLRPLFTSLIAYQTP 146
F P T A P
Sbjct: 304 PLKFSSPATTMAPAQDCP 357
>AW720177
Length = 505
Score = 33.5 bits (75), Expect = 0.081
Identities = 29/83 (34%), Positives = 38/83 (44%)
Frame = +2
Query: 54 PPHVAAPRDASVTSISLFLRNRHFPFHNRPIVRSNSPLHATHLPKTIMTSNLPHASNTRH 113
PPH R +SL LR++ P+ +SPL LP+ T LPH
Sbjct: 215 PPHRFLRR------VSLLLRHQPTLSRPLPLPLRHSPLR--RLPRA--TVPLPHPP---- 352
Query: 114 PSSFSPSPIKTLLPHSHPFLRPL 136
PS +P+P + LP HP L PL
Sbjct: 353 PSRXAPNPKRPRLPPRHPRLPPL 421
>TC12700 similar to UP|P93390 (P93390) Phosphate/phosphoenolpyruvate
translocator, partial (9%)
Length = 442
Score = 33.1 bits (74), Expect = 0.11
Identities = 27/81 (33%), Positives = 33/81 (40%), Gaps = 1/81 (1%)
Frame = +3
Query: 54 PPHVAAPRDASVTSISLFLRNRHF-PFHNRPIVRSNSPLHATHLPKTIMTSNLPHASNTR 112
PPH + S+ SISL R+R P P SP + P T P AS
Sbjct: 21 PPHTHTTQSCSLLSISLSFRSRKCKPRRRSPPSPPPSPTATSTAPTTSGDLPSPSASLPN 200
Query: 113 HPSSFSPSPIKTLLPHSHPFL 133
P++ LLPH PFL
Sbjct: 201 SPTAAQIQMAPLLLPHP-PFL 260
>AU089273
Length = 420
Score = 32.0 bits (71), Expect = 0.24
Identities = 23/82 (28%), Positives = 30/82 (36%), Gaps = 21/82 (25%)
Frame = +1
Query: 70 LFLRNRHFPFHNRPIVRSNSPLHATHL---------------------PKTIMTSNLPHA 108
L + NR P N+ + P H THL P T+ S + H
Sbjct: 151 LLMPNRPQPHQNQCQIFQTPPQHKTHLIPKLPTYSNLNYQTYQSPTTFPSTLTASRIFHN 330
Query: 109 SNTRHPSSFSPSPIKTLLPHSH 130
S T H SS +P T P +H
Sbjct: 331 SPTVHASSSAPPEKSTPTPETH 396
>TC7849 similar to GB|AAN18126.1|23308313|BT000557 At2g10940/F15K19.1
{Arabidopsis thaliana;}, partial (55%)
Length = 1157
Score = 31.6 bits (70), Expect = 0.31
Identities = 17/49 (34%), Positives = 23/49 (46%)
Frame = +1
Query: 166 TSYSGESHCPYLCITHILIFSFLSLHCLLKMASNPTSPNHSSSSSGSDP 214
+SYS +HCP C TH +S+ HSSS SG++P
Sbjct: 319 SSYSSSNHCPSGCETH--------------SSSDSRCDTHSSSGSGAEP 423
>AV406930
Length = 433
Score = 31.2 bits (69), Expect = 0.40
Identities = 36/130 (27%), Positives = 54/130 (40%), Gaps = 12/130 (9%)
Frame = +1
Query: 50 LNLYPPHVAAPRDASVTSISLFLRNRHF------------PFHNRPIVRSNSPLHATHLP 97
L+L P H + P +S+++++L HF P ++ + S P+ H P
Sbjct: 4 LHLPPLHSSHPPHSSISALAL--APPHFLAPPPPPQPPNPPSISQNLSISTHPITPFHTP 177
Query: 98 KTIMTSNLPHASNTRHPSSFSPSPIKTLLPHSHPFLRPLFTSLIAYQTPSAHFRSGAVAY 157
+ + NLP P SFS P + P S P L P PS+ R G+ +
Sbjct: 178 LS-HSFNLPLPKPASIPLSFSALPSPSPSPSSSPTLIP--------PPPSSSRRRGSCS- 327
Query: 158 HPFRYPLFTS 167
P PLF S
Sbjct: 328 -PMNSPLFVS 354
>BI418438
Length = 431
Score = 30.4 bits (67), Expect = 0.69
Identities = 18/44 (40%), Positives = 26/44 (58%)
Frame = +3
Query: 176 YLCITHILIFSFLSLHCLLKMASNPTSPNHSSSSSGSDPDATAA 219
+L ++ +L SFL L L AS+ + +H S + S PDATAA
Sbjct: 144 FLTMSPLLPLSFLLLILTLSSASHSHNNHHHHSPTPSLPDATAA 275
>TC18336 similar to UP|Q84KA9 (Q84KA9) RING/C3HC4/PHD zinc finger-like
protein, partial (15%)
Length = 761
Score = 30.4 bits (67), Expect = 0.69
Identities = 14/24 (58%), Positives = 15/24 (62%)
Frame = -2
Query: 119 PSPIKTLLPHSHPFLRPLFTSLIA 142
PSP LPH HP LRP TS +A
Sbjct: 505 PSPTSPPLPHHHPSLRPKTTS*LA 434
>TC9284 weakly similar to UP|Q9LPY0 (Q9LPY0) T23J18.22, partial (33%)
Length = 898
Score = 29.6 bits (65), Expect = 1.2
Identities = 22/72 (30%), Positives = 33/72 (45%), Gaps = 3/72 (4%)
Frame = +1
Query: 163 PLFTSYSGESHCPYLCITHILIFSFLSLHCLLKMASNPTSPNHSSSSSGSDPD---ATAA 219
PL +S+ G S Y + H ++ S PT+ N S+S S S+PD A++A
Sbjct: 73 PLRSSHCGSSSPSYGAVCHRILLS------------TPTTTNESASFSPSNPDPNPASSA 216
Query: 220 GGLRLEAPEIPP 231
+ A PP
Sbjct: 217 STTKPSAATSPP 252
>AV766417
Length = 440
Score = 29.3 bits (64), Expect = 1.5
Identities = 16/48 (33%), Positives = 21/48 (43%)
Frame = +3
Query: 83 PIVRSNSPLHATHLPKTIMTSNLPHASNTRHPSSFSPSPIKTLLPHSH 130
P S+ H T+ P +T PH S + PS+ P P P SH
Sbjct: 195 PTKASDPIAHTTNAPPLSLTQYPPHVSRAQPPSAHGPRP----SPFSH 326
>TC14545 similar to UP|Q9FT78 (Q9FT78) P23 co-chaperone, partial (46%)
Length = 1127
Score = 29.3 bits (64), Expect = 1.5
Identities = 21/61 (34%), Positives = 29/61 (47%), Gaps = 6/61 (9%)
Frame = -1
Query: 79 FHNRPIVRSNSPLHATHLPKTIMTSNLPHASNTR----HPS--SFSPSPIKTLLPHSHPF 132
FH+ S P H+TH K+ TS H +T+ HPS FS + L+ HPF
Sbjct: 596 FHSSQSSHSTHPSHSTHPSKSSKTSITAHTHSTKFREVHPSHIRFSNNIFFILI---HPF 426
Query: 133 L 133
+
Sbjct: 425 I 423
>BP038767
Length = 561
Score = 29.3 bits (64), Expect = 1.5
Identities = 22/72 (30%), Positives = 33/72 (45%), Gaps = 4/72 (5%)
Frame = +1
Query: 176 YLCITHILIF---SFLSLHCLLKMASNPTSPNHSSSSSGSDPD-ATAAGGLRLEAPEIPP 231
Y+ +HI+ F S +SL + N T+PNH + +S S P T + P PP
Sbjct: 151 YIQFSHIIFFPIQSSISLFHHARSRQNSTAPNHHAPNSNSHPKIRTRRNQQQNSLPVAPP 330
Query: 232 YRLKTYATLPLP 243
AT+ +P
Sbjct: 331 EE----ATISVP 354
>TC9777 similar to UP|SOX_ARATH (Q9SJA7) Potential sarcosine oxidase ,
partial (38%)
Length = 820
Score = 29.3 bits (64), Expect = 1.5
Identities = 27/79 (34%), Positives = 32/79 (40%), Gaps = 9/79 (11%)
Frame = -1
Query: 94 THLPKTIMTSNLPHASNTRHPSSFSPSPI-------KTLLPHSHPFLRPLFTSLIAYQTP 146
TH PKT PH S T HP + S +P K P +H P + TP
Sbjct: 427 THDPKTRHPP--PHPSQTTHPRNPSQNPHQES*NTGKIASPQAH--WNPPHRKISLATTP 260
Query: 147 SAH--FRSGAVAYHPFRYP 163
SAH SG A +P P
Sbjct: 259 SAHPASHSGPTASYPGHTP 203
>TC12321 similar to UP|Q15415 (Q15415) YRRM2, partial (5%)
Length = 812
Score = 29.3 bits (64), Expect = 1.5
Identities = 28/92 (30%), Positives = 36/92 (38%), Gaps = 1/92 (1%)
Frame = +1
Query: 101 MTSNLPHASNTRHPSSFS-PSPIKTLLPHSHPFLRPLFTSLIAYQTPSAHFRSGAVAYHP 159
+ S P N ++P FS PSP + P S F+ P F+SL P
Sbjct: 205 LNSLSPIIINKQNPLPFSTPSPSLSPPPKSFHFISPFFSSLT----------------QP 336
Query: 160 FRYPLFTSYSGESHCPYLCITHILIFSFLSLH 191
F +PL + C H L FS SLH
Sbjct: 337 FTFPLTLYFF*T*KC-----NHSLPFSLSSLH 417
>AV407514
Length = 329
Score = 28.9 bits (63), Expect = 2.0
Identities = 18/57 (31%), Positives = 27/57 (46%)
Frame = +2
Query: 161 RYPLFTSYSGESHCPYLCITHILIFSFLSLHCLLKMASNPTSPNHSSSSSGSDPDAT 217
R PL T + H + I +LIF F S H MAS + +H +++ P +T
Sbjct: 2 RLPLLTR-TATGHAT-ITIVPVLIFLFFSFHVQTTMASPKLASSHGDAATSFSPAST 166
>TC13364 similar to UP|Q9LUM3 (Q9LUM3) Subtilisin proteinase-like protein,
partial (13%)
Length = 514
Score = 28.9 bits (63), Expect = 2.0
Identities = 20/59 (33%), Positives = 27/59 (44%)
Frame = +2
Query: 114 PSSFSPSPIKTLLPHSHPFLRPLFTSLIAYQTPSAHFRSGAVAYHPFRYPLFTSYSGES 172
P SFSPS + LPHSH L L+ ++ + +S PF P+ T S S
Sbjct: 77 PLSFSPSLSHSSLPHSHYHLH---HHLLTQKSKPSSSKSNMKPSLPFSQPISTGTSPPS 244
>BP066856
Length = 485
Score = 28.1 bits (61), Expect = 3.4
Identities = 17/58 (29%), Positives = 26/58 (44%), Gaps = 7/58 (12%)
Frame = +2
Query: 64 SVTSISLFLRNRHFPFHNR-------PIVRSNSPLHATHLPKTIMTSNLPHASNTRHP 114
SV + L+ H P HNR PI+R + P+ T +P ++ L S+ P
Sbjct: 272 SVKHLQ*LLQQVHGPTHNRHSTELLPPIIRDDEPMGHTAIPPHVILHXLSRISSRDPP 445
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.322 0.138 0.434
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,677,087
Number of Sequences: 28460
Number of extensions: 187948
Number of successful extensions: 1495
Number of sequences better than 10.0: 86
Number of HSP's better than 10.0 without gapping: 1474
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1493
length of query: 494
length of database: 4,897,600
effective HSP length: 94
effective length of query: 400
effective length of database: 2,222,360
effective search space: 888944000
effective search space used: 888944000
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 57 (26.6 bits)
Lotus: description of TM0034b.2