BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0031.12
(227 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP056483 41 1e-04
TC10380 similar to UP|Q8S8U2 (Q8S8U2) Ycf1 protein, partial (40%) 34 0.024
TC8758 similar to GB|AAC77862.2|20197419|AC005623 Argonaute (AGO... 34 0.024
TC18497 similar to UP|Q9FJX5 (Q9FJX5) Genomic DNA, chromosome 5,... 32 0.12
TC16154 weakly similar to UP|E75C_DROME (P13055) Ecdysone-induce... 31 0.20
TC10255 30 0.34
CB827703 30 0.45
TC14320 similar to UP|Q41111 (Q41111) Dehydrin, partial (56%) 29 0.59
BP030023 27 2.9
TC15976 weakly similar to UP|Q9LIH5 (Q9LIH5) Genomic DNA, chromo... 27 3.8
BP082331 27 3.8
TC15584 26 5.0
TC11310 similar to UP|BAD11331 (BAD11331) BRI1-KD interacting pr... 26 5.0
AV780992 26 5.0
TC17161 homologue to UP|THIF_PEA (P29450) Thioredoxin F-type, ch... 26 5.0
TC15527 similar to UP|AAR87166 (AAR87166) Expressed protein (Wit... 26 5.0
AV767728 26 5.0
AW164114 26 5.0
BP041040 26 6.5
TC7927 similar to UP|Q93YR3 (Q93YR3) HSP associated protein like... 26 6.5
>BP056483
Length = 333
Score = 41.2 bits (95), Expect = 1e-04
Identities = 18/20 (90%), Positives = 20/20 (100%)
Frame = -1
Query: 170 SLTEEENAERVRTRNPNQRE 189
S++EEENAERVRTRNPNQRE
Sbjct: 84 SMSEEENAERVRTRNPNQRE 25
>TC10380 similar to UP|Q8S8U2 (Q8S8U2) Ycf1 protein, partial (40%)
Length = 5664
Score = 33.9 bits (76), Expect = 0.024
Identities = 33/106 (31%), Positives = 55/106 (51%), Gaps = 5/106 (4%)
Frame = +2
Query: 64 RNSKINKITENAAPIITETTKKREENHKT---LPERKHRASNESKAKTQSKLKKMKRKTQ 120
+N K IT+N PII E+T + +KT L E+K +A N AKT+ +KK++R T+
Sbjct: 2990 QNKKNFPITKNN-PIIYESTILIQAINKTNCSLTEKKIKAIN---AKTKKIIKKIERMTK 3157
Query: 121 --NAGQIMKQTSEKIVKKKQKINKKSKTIFTTSTERAKQRNRRITH 164
G ++ + + KK N K + + ++RN ++TH
Sbjct: 3158 ENKGGFLISEINSN--SKKTSSNTKGLELEKKILQILQRRNVQLTH 3289
>TC8758 similar to GB|AAC77862.2|20197419|AC005623 Argonaute (AGO1)-like
protein {Arabidopsis thaliana;} , partial (32%)
Length = 1142
Score = 33.9 bits (76), Expect = 0.024
Identities = 14/19 (73%), Positives = 17/19 (88%)
Frame = -2
Query: 170 SLTEEENAERVRTRNPNQR 188
S++EEENAER +RNPNQR
Sbjct: 58 SMSEEENAERYESRNPNQR 2
>TC18497 similar to UP|Q9FJX5 (Q9FJX5) Genomic DNA, chromosome 5, TAC
clone:K9I9 (AT5g67480/K9I9_4), partial (61%)
Length = 696
Score = 31.6 bits (70), Expect = 0.12
Identities = 16/52 (30%), Positives = 30/52 (56%)
Frame = +2
Query: 138 QKINKKSKTIFTTSTERAKQRNRRITHGEVRFSLTEEENAERVRTRNPNQRE 189
+KI K K + + +A +++ I E+ S+ +EEN+++ R R N+RE
Sbjct: 374 RKILKNFKAVSVSEGWKAMKQSHPILEKEILESVIDEENSKKERIRKINERE 529
>TC16154 weakly similar to UP|E75C_DROME (P13055) Ecdysone-induced protein
75B isoform C (E75-C), partial (3%)
Length = 815
Score = 30.8 bits (68), Expect = 0.20
Identities = 21/61 (34%), Positives = 32/61 (52%), Gaps = 10/61 (16%)
Frame = -3
Query: 51 NNHKIQRNEQKYTRNSKI--NKITENAAP------IITETTKKREENHKTLPERK--HRA 100
N++KIQ N K+T+ KI N+ T+ P + + +RE HK LP + HR+
Sbjct: 696 NSNKIQNNLLKFTKKIKIQQNRRTKKNNPNSESIKTLRRRSHRRERTHKLLPHPRTDHRS 517
Query: 101 S 101
S
Sbjct: 516 S 514
>TC10255
Length = 539
Score = 30.0 bits (66), Expect = 0.34
Identities = 30/135 (22%), Positives = 53/135 (39%), Gaps = 14/135 (10%)
Frame = -2
Query: 53 HKIQRNEQKYTRNSKINKITENAAPIITETTKKREENHKTLPERKHRASNESKAKTQSKL 112
++I+ N++K TR+ ++ T N A KK + KT H + KL
Sbjct: 445 NRIKDNQKKKTRD*RL---TSNRAKEKKTK*KKNRDEAKTKTATAHVRRGLGQHPKDQKL 275
Query: 113 KKMKRKTQNAGQIMKQT--------------SEKIVKKKQKINKKSKTIFTTSTERAKQR 158
++ + + Q KQT K+ K KI + + + TTS+ Q+
Sbjct: 274 RRKRGQEQPGDSH*KQTFTPGKILTHAASKGQSKVYKAAAKITRDTNPMATTSSPSIVQK 95
Query: 159 NRRITHGEVRFSLTE 173
N I + + + E
Sbjct: 94 NIAIQNSTYQIASQE 50
>CB827703
Length = 555
Score = 29.6 bits (65), Expect = 0.45
Identities = 15/34 (44%), Positives = 19/34 (55%)
Frame = -2
Query: 52 NHKIQRNEQKYTRNSKINKITENAAPIITETTKK 85
NHKI RN+ K + SK K+ + P IT T K
Sbjct: 146 NHKIHRNQSKPSYFSKFPKVKQ*RKP*ITHKTSK 45
>TC14320 similar to UP|Q41111 (Q41111) Dehydrin, partial (56%)
Length = 1231
Score = 29.3 bits (64), Expect = 0.59
Identities = 24/101 (23%), Positives = 43/101 (41%), Gaps = 7/101 (6%)
Frame = +3
Query: 38 KTEDQASNACKGNNNHKIQRNEQKYTRNSKINKITENAAPIITETTKKREENHKTLPERK 97
+T+D+ G + + E+ + K++E E + E+ H TL E+
Sbjct: 405 ETQDRGVFDFLGKKKEEEKPQEEVIVTEFEKVKVSEEPEKKKEEVDHEGEKKHSTLLEKL 584
Query: 98 HR-------ASNESKAKTQSKLKKMKRKTQNAGQIMKQTSE 131
HR +S+E + K + KK K+K + +K T E
Sbjct: 585 HRSDSSSSSSSDEEEGKEGEEKKKKKKKEKKE---VKSTDE 698
>BP030023
Length = 538
Score = 26.9 bits (58), Expect = 2.9
Identities = 18/64 (28%), Positives = 31/64 (48%), Gaps = 11/64 (17%)
Frame = +3
Query: 81 ETTKKREENHKTLPERKHRASN----ESKAKTQS-------KLKKMKRKTQNAGQIMKQT 129
+T K REE + +KH A N + K T+S +KM+ + NA +I +
Sbjct: 75 KTAKAREEKEQNRKIKKHSAQNRK*KQGKNTTKSITCRGSDTARKMQGRRNNAAKISNRQ 254
Query: 130 SEKI 133
S+++
Sbjct: 255 SQQL 266
>TC15976 weakly similar to UP|Q9LIH5 (Q9LIH5) Genomic DNA, chromosome 3, TAC
clone:K24M9, partial (13%)
Length = 665
Score = 26.6 bits (57), Expect = 3.8
Identities = 18/68 (26%), Positives = 30/68 (43%)
Frame = +1
Query: 69 NKITENAAPIITETTKKREENHKTLPERKHRASNESKAKTQSKLKKMKRKTQNAGQIMKQ 128
NKIT+ + P+ + + K + K K +K++ + T GQI K
Sbjct: 43 NKITKESGPLASMDLSGGPDEIKNTKDVK--GMRAFKGSLVEFIKELLKPTWKEGQITKD 216
Query: 129 TSEKIVKK 136
++IVKK
Sbjct: 217 DYKEIVKK 240
>BP082331
Length = 494
Score = 26.6 bits (57), Expect = 3.8
Identities = 24/101 (23%), Positives = 44/101 (42%), Gaps = 2/101 (1%)
Frame = +2
Query: 78 IITETTKKREENHKTLPERKHRASNESKAKTQ--SKLKKMKRKTQNAGQIMKQTSEKIVK 135
I++E E+ E K S +K + +K+KKM++ ++ + E +
Sbjct: 116 ILSEIQAPAEKRLVFHSELKRLGSGGAKVLRELGNKIKKMEKLGPE--DLLYEVHEAAEE 289
Query: 136 KKQKINKKSKTIFTTSTERAKQRNRRITHGEVRFSLTEEEN 176
+QKI+KKS + + R R + F++ EE N
Sbjct: 290 LQQKIDKKSYLLVNSERWEIGNRPREEVDPQGLFTMHEERN 412
>TC15584
Length = 797
Score = 26.2 bits (56), Expect = 5.0
Identities = 14/33 (42%), Positives = 18/33 (54%)
Frame = -2
Query: 100 ASNESKAKTQSKLKKMKRKTQNAGQIMKQTSEK 132
AS SKA + KM R T + Q+M +TS K
Sbjct: 751 ASKSSKAPYLATTLKMMRHTNDKKQVMMKTSNK 653
>TC11310 similar to UP|BAD11331 (BAD11331) BRI1-KD interacting protein 102
(Fragment), partial (13%)
Length = 667
Score = 26.2 bits (56), Expect = 5.0
Identities = 19/58 (32%), Positives = 32/58 (54%)
Frame = +1
Query: 81 ETTKKREENHKTLPERKHRASNESKAKTQSKLKKMKRKTQNAGQIMKQTSEKIVKKKQ 138
E +K+++ K + +S+E KA+ + K KK K K ++ + Q SEK KKK+
Sbjct: 79 EKKEKKKKKKKDQENGEVASSDEEKAEKEKK-KKHKVKVEDGSPDLDQ-SEKKKKKKK 246
>AV780992
Length = 561
Score = 26.2 bits (56), Expect = 5.0
Identities = 11/19 (57%), Positives = 13/19 (67%)
Frame = -1
Query: 81 ETTKKREENHKTLPERKHR 99
E KKREE +T PER +R
Sbjct: 444 EVKKKREEEEETEPERNYR 388
>TC17161 homologue to UP|THIF_PEA (P29450) Thioredoxin F-type, chloroplast
precursor (TRX-F), partial (72%)
Length = 850
Score = 26.2 bits (56), Expect = 5.0
Identities = 16/47 (34%), Positives = 24/47 (51%)
Frame = -3
Query: 94 PERKHRASNESKAKTQSKLKKMKRKTQNAGQIMKQTSEKIVKKKQKI 140
P+ H +SN K + KLKKMK+K Q K + K+++ I
Sbjct: 209 PQHYHFSSNSHFCK-RKKLKKMKKKNQVVAWKKKHYQTRSWKEERSI 72
>TC15527 similar to UP|AAR87166 (AAR87166) Expressed protein (With
alternative splicing), partial (8%)
Length = 562
Score = 26.2 bits (56), Expect = 5.0
Identities = 14/37 (37%), Positives = 20/37 (53%)
Frame = -3
Query: 81 ETTKKREENHKTLPERKHRASNESKAKTQSKLKKMKR 117
E+ + EE+ +PER + A + KA S L MKR
Sbjct: 332 ESLRDDEEDEDEIPERLNTALSLLKALLTSSLSSMKR 222
>AV767728
Length = 332
Score = 26.2 bits (56), Expect = 5.0
Identities = 17/58 (29%), Positives = 27/58 (46%)
Frame = -2
Query: 115 MKRKTQNAGQIMKQTSEKIVKKKQKINKKSKTIFTTSTERAKQRNRRITHGEVRFSLT 172
+K Q G Q E++ KKK ++++ + +QRNRR G+ FS T
Sbjct: 304 LKNSAQKLGTFSSQALEQLKKKKNVGARQTRMLQQELDMLKRQRNRR-GGGDPGFSFT 134
>AW164114
Length = 297
Score = 26.2 bits (56), Expect = 5.0
Identities = 16/78 (20%), Positives = 31/78 (39%)
Frame = +2
Query: 45 NACKGNNNHKIQRNEQKYTRNSKINKITENAAPIITETTKKREENHKTLPERKHRASNES 104
N+ + +++ + E K N++ N K + + E+ + + +
Sbjct: 2 NSARASHDQSYRAGETMGRTEEKGNQMMGNIGEKAQAAKDKTAQTAQAAKEKTAQGAQAA 181
Query: 105 KAKTQSKLKKMKRKTQNA 122
K KTQ + K KTQ A
Sbjct: 182 KEKTQHTAQAAKDKTQQA 235
>BP041040
Length = 550
Score = 25.8 bits (55), Expect = 6.5
Identities = 12/36 (33%), Positives = 20/36 (55%)
Frame = +3
Query: 62 YTRNSKINKITENAAPIITETTKKREENHKTLPERK 97
Y + K N+I + A +++ T EN+ T PER+
Sbjct: 435 YLGDEKANEIVKEALYLVSIGTNDFLENYYTFPERR 542
>TC7927 similar to UP|Q93YR3 (Q93YR3) HSP associated protein like, partial
(56%)
Length = 1116
Score = 25.8 bits (55), Expect = 6.5
Identities = 17/73 (23%), Positives = 29/73 (39%)
Frame = +1
Query: 51 NNHKIQRNEQKYTRNSKINKITENAAPIITETTKKREENHKTLPERKHRASNESKAKTQS 110
N HKI+ + +KY E K E +T ER+ R + A ++
Sbjct: 259 NAHKIEEHRRKY------------------ERLHKEREEKRTERERQRRRAEAQAAYEKA 384
Query: 111 KLKKMKRKTQNAG 123
K ++ ++N G
Sbjct: 385 KKQEQSSSSRNPG 423
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.307 0.122 0.323
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,016,654
Number of Sequences: 28460
Number of extensions: 31830
Number of successful extensions: 235
Number of sequences better than 10.0: 47
Number of HSP's better than 10.0 without gapping: 232
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 235
length of query: 227
length of database: 4,897,600
effective HSP length: 87
effective length of query: 140
effective length of database: 2,421,580
effective search space: 339021200
effective search space used: 339021200
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.7 bits)
S2: 53 (25.0 bits)
Lotus: description of TM0031.12