BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0028b.3
(288 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC8112 similar to UP|ODPA_PEA (P52902) Pyruvate dehydrogenase E1... 582 e-167
TC9451 homologue to UP|O24457 (O24457) Pyruvate dehydrogenase E1... 99 5e-36
TC12700 similar to UP|P93390 (P93390) Phosphate/phosphoenolpyruv... 30 0.62
TC18837 weakly similar to UP|Q39856 (Q39856) Epoxide hydrolase ... 29 1.1
TC17487 UP|CAE45597 (CAE45597) SAR DNA-binding protein-like prot... 28 1.8
AV409182 28 2.4
TC10816 homologue to UP|CAE45585 (CAE45585) Coatomer alpha subun... 28 2.4
TC8606 similar to UP|AAQ82688 (AAQ82688) Epa4p, partial (7%) 28 2.4
AV426624 27 3.1
AV426208 27 3.1
TC10062 similar to BAD08700 (BAD08700) Cold shock domain protein... 27 3.1
AV778595 27 4.0
TC8181 homologue to UP|O23310 (O23310) CCAAT-binding transcripti... 27 4.0
TC9682 similar to UP|Q8VZC0 (Q8VZC0) DTDP-glucose 4-6-dehydratas... 27 4.0
BP048961 27 4.0
TC19949 UP|Q8H0D4 (Q8H0D4) Rac GTPase, partial (33%) 27 5.3
TC19072 homologue to UP|CC37_CAEEL (O02108) Probable Hsp90 co-ch... 26 6.9
TC18988 similar to UP|FUS_MOUSE (P56959) RNA-binding protein FUS... 26 6.9
TC12269 homologue to UP|Q8GZT8 (Q8GZT8) PTEN-like protein, parti... 26 6.9
TC8133 similar to GB|BAA97157.1|8809606|AB018117 ethylene respon... 26 9.0
>TC8112 similar to UP|ODPA_PEA (P52902) Pyruvate dehydrogenase E1 component
alpha subunit, mitochondrial precursor (PDHE1-A) ,
partial (90%)
Length = 1816
Score = 582 bits (1499), Expect = e-167
Identities = 288/288 (100%), Positives = 288/288 (100%)
Frame = +3
Query: 1 MALSRLASSSSSSSSTVGSKFLKPLTAAFSLRRSISSDSNPITIETSVPFTAHNCDPPSR 60
MALSRLASSSSSSSSTVGSKFLKPLTAAFSLRRSISSDSNPITIETSVPFTAHNCDPPSR
Sbjct: 117 MALSRLASSSSSSSSTVGSKFLKPLTAAFSLRRSISSDSNPITIETSVPFTAHNCDPPSR 296
Query: 61 SVETSTAELHSFFRQMATMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAIGMEAGITKKD 120
SVETSTAELHSFFRQMATMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAIGMEAGITKKD
Sbjct: 297 SVETSTAELHSFFRQMATMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAIGMEAGITKKD 476
Query: 121 AIITAYRDHCTFLGRGGTLLEIFSELMGRVDGCSKGKGGSMHFYRKETNFYGGHGIVGAQ 180
AIITAYRDHCTFLGRGGTLLEIFSELMGRVDGCSKGKGGSMHFYRKETNFYGGHGIVGAQ
Sbjct: 477 AIITAYRDHCTFLGRGGTLLEIFSELMGRVDGCSKGKGGSMHFYRKETNFYGGHGIVGAQ 656
Query: 181 VPLGCGVAFAQKYSKDEAVTFALYGDGAANQGQLFEALNIAALWDLPAILVCENNHYGMG 240
VPLGCGVAFAQKYSKDEAVTFALYGDGAANQGQLFEALNIAALWDLPAILVCENNHYGMG
Sbjct: 657 VPLGCGVAFAQKYSKDEAVTFALYGDGAANQGQLFEALNIAALWDLPAILVCENNHYGMG 836
Query: 241 TAEWRAAKSPAYYKRGDYVPGLKVDGMDVLAVKQACKFAKEHALKNGP 288
TAEWRAAKSPAYYKRGDYVPGLKVDGMDVLAVKQACKFAKEHALKNGP
Sbjct: 837 TAEWRAAKSPAYYKRGDYVPGLKVDGMDVLAVKQACKFAKEHALKNGP 980
>TC9451 homologue to UP|O24457 (O24457) Pyruvate dehydrogenase E1 alpha
subunit (At1g01090/T25K16_8) , partial (52%)
Length = 1082
Score = 99.0 bits (245), Expect(2) = 5e-36
Identities = 45/111 (40%), Positives = 68/111 (60%)
Frame = +1
Query: 86 ADSLYKAKLIRGFCHLYDGQEAVAIGMEAGITKKDAIITAYRDHCTFLGRGGTLLEIFSE 145
A+ Y+ K+ GF HLY+GQEAV+ G + K+D++++ YRDH L +G + SE
Sbjct: 517 AEMYYRGKMF-GFVHLYNGQEAVSTGFIKLLKKEDSVVSTYRDHVHALSKGVPARAVMSE 693
Query: 146 LMGRVDGCSKGKGGSMHFYRKETNFYGGHGIVGAQVPLGCGVAFAQKYSKD 196
L G+ G +G+GGSMH + KE N GG +G +P+ G AF+ KY ++
Sbjct: 694 LFGKATGVCRGQGGSMHMFSKEHNVLGGFAFIGEGIPVATGAAFSSKYRRE 846
Score = 68.6 bits (166), Expect(2) = 5e-36
Identities = 32/69 (46%), Positives = 43/69 (61%), Gaps = 2/69 (2%)
Frame = +2
Query: 199 VTFALYGDGAANQGQLFEALNIAALWDLPAILVCENNHYGMGTAEWRAAKSPAYYKRGDY 258
VT A +GDG N GQ +E LN+AALW LP + V ENN + +G + RA P +K+G
Sbjct: 875 VTLAFFGDGTCNNGQFYECLNMAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPA 1054
Query: 259 --VPGLKVD 265
+PG+ VD
Sbjct: 1055FGMPGVHVD 1081
>TC12700 similar to UP|P93390 (P93390) Phosphate/phosphoenolpyruvate
translocator, partial (9%)
Length = 442
Score = 29.6 bits (65), Expect = 0.62
Identities = 20/57 (35%), Positives = 33/57 (57%)
Frame = +2
Query: 8 SSSSSSSSTVGSKFLKPLTAAFSLRRSISSDSNPITIETSVPFTAHNCDPPSRSVET 64
+SSSSSSS + ++ F R ++SDS+P +SVP +A + +P S ++T
Sbjct: 230 ASSSSSSSFPRRSWHLANSSPFKFR-PLASDSSPPKAASSVPDSAGSAEPSSSLMKT 397
>TC18837 weakly similar to UP|Q39856 (Q39856) Epoxide hydrolase , partial
(89%)
Length = 1100
Score = 28.9 bits (63), Expect = 1.1
Identities = 21/65 (32%), Positives = 30/65 (45%), Gaps = 3/65 (4%)
Frame = +2
Query: 5 RLASSSSSSSSTVGSKFLKPLTAAFSLRRSISSDSNPIT---IETSVPFTAHNCDPPSRS 61
+LA + S SSST F P +FS ++ +P+T T P + PPS S
Sbjct: 50 QLAQAQSFSSSTASRSFGTPGATSFSPSPPSATAQSPLTSAATATPTPRPTPHPTPPSTS 229
Query: 62 VETST 66
TS+
Sbjct: 230 SATSS 244
>TC17487 UP|CAE45597 (CAE45597) SAR DNA-binding protein-like protein,
complete
Length = 1794
Score = 28.1 bits (61), Expect = 1.8
Identities = 17/49 (34%), Positives = 26/49 (52%)
Frame = +3
Query: 2 ALSRLASSSSSSSSTVGSKFLKPLTAAFSLRRSISSDSNPITIETSVPF 50
+LSRL S++SSS+ +G LKP ++ S R + + ET F
Sbjct: 21 SLSRLCSAASSSTLALGFMPLKPFFSSSS*TRFVGGVKMLVLFETPAGF 167
>AV409182
Length = 312
Score = 27.7 bits (60), Expect = 2.4
Identities = 19/64 (29%), Positives = 33/64 (50%), Gaps = 11/64 (17%)
Frame = +1
Query: 184 GCGVAFAQK-----YSKDEA-----VTFALYGDGAANQGQLFEALNIAALWDL-PAILVC 232
G G+A A+K ++K ++ T+ + GDG +G EA ++A W L I +
Sbjct: 16 GVGLALAEKHLAARFNKPDSEIVDHYTYVILGDGCQMEGISNEACSLAGHWGLGKLIALY 195
Query: 233 ENNH 236
++NH
Sbjct: 196 DDNH 207
>TC10816 homologue to UP|CAE45585 (CAE45585) Coatomer alpha subunit-like
protein, partial (20%)
Length = 979
Score = 27.7 bits (60), Expect = 2.4
Identities = 18/54 (33%), Positives = 28/54 (51%)
Frame = +3
Query: 1 MALSRLASSSSSSSSTVGSKFLKPLTAAFSLRRSISSDSNPITIETSVPFTAHN 54
+++S SSSSSSSS+ S + S+ + S+ N IT S+P + N
Sbjct: 60 ISISTKPSSSSSSSSSSSSSLI-------SILSHLPSNPNRITASLSIPISRSN 200
>TC8606 similar to UP|AAQ82688 (AAQ82688) Epa4p, partial (7%)
Length = 1008
Score = 27.7 bits (60), Expect = 2.4
Identities = 21/58 (36%), Positives = 30/58 (51%), Gaps = 3/58 (5%)
Frame = +1
Query: 4 SRLASSSSSSSSTVGSKFLKPLTAAFSLRRSISSD---SNPITIETSVPFTAHNCDPP 58
S +SSS SSSST S P +++ S S SS S+P + +S PF++ P
Sbjct: 289 SSSSSSSLSSSSTPSSSSSSPPSSSSSSSSSSSSSSSWSSPSSSSSSSPFSSGPAPAP 462
>AV426624
Length = 422
Score = 27.3 bits (59), Expect = 3.1
Identities = 21/61 (34%), Positives = 29/61 (47%), Gaps = 6/61 (9%)
Frame = -2
Query: 4 SRLASSSSSSSSTVGSKFLKPLTAAFSLRRSISSDSNPITIET------SVPFTAHNCDP 57
S +SSSSSSSS+ S +P ++ L++ N T+ T S P NC P
Sbjct: 397 SSSSSSSSSSSSSSSSSL*EPFYSSSHLKKQKPQIFNLRTLMTHTQTRYSHPVRFPNCAP 218
Query: 58 P 58
P
Sbjct: 217 P 215
>AV426208
Length = 428
Score = 27.3 bits (59), Expect = 3.1
Identities = 18/50 (36%), Positives = 25/50 (50%)
Frame = +3
Query: 7 ASSSSSSSSTVGSKFLKPLTAAFSLRRSISSDSNPITIETSVPFTAHNCD 56
+S+SSSSSST K + P +L RS+ +D P+ P CD
Sbjct: 255 SSNSSSSSSTSPHKIISPNKFV*NL*RSLPTDGFPMIELVFSPLHDLACD 404
>TC10062 similar to BAD08700 (BAD08700) Cold shock domain protein 2, partial
(66%)
Length = 481
Score = 27.3 bits (59), Expect = 3.1
Identities = 20/71 (28%), Positives = 32/71 (44%)
Frame = -3
Query: 7 ASSSSSSSSTVGSKFLKPLTAAFSLRRSISSDSNPITIETSVPFTAHNCDPPSRSVETST 66
++++SSS++TV + + S SIS+ S T+ +A S S TST
Sbjct: 464 SATTSSSTATVSTATTITTASTVSASSSISATSASSPTVTASAISASTITASSASTATST 285
Query: 67 AELHSFFRQMA 77
A S +A
Sbjct: 284 ASAASSLHSLA 252
>AV778595
Length = 594
Score = 26.9 bits (58), Expect = 4.0
Identities = 26/90 (28%), Positives = 41/90 (44%), Gaps = 14/90 (15%)
Frame = +2
Query: 7 ASSSSSSSSTVGSKFLKPL-----------TAAFSLRRSISS--DSNPITIETSVPFTAH 53
ASSSSSSSS+ GS + L + +L R+++S + P + S+ +
Sbjct: 92 ASSSSSSSSSGGSSEQQELLLWKSDPQPESIVSVTLARAVTSLLTTRPTKLHDSISRLSP 271
Query: 54 NCDPPS-RSVETSTAELHSFFRQMATMRRM 82
+ PPS S+E S H+F A +
Sbjct: 272 SPTPPSTASLEHSLRFFHTFVTDAAAANNL 361
>TC8181 homologue to UP|O23310 (O23310) CCAAT-binding transcription
factor subunit A (At4g14540), partial (65%)
Length = 1223
Score = 26.9 bits (58), Expect = 4.0
Identities = 15/57 (26%), Positives = 29/57 (50%), Gaps = 1/57 (1%)
Frame = +3
Query: 41 PITIETSVPFTAHNCDPPSRSVETSTAELHSFFRQ-MATMRRMEIAADSLYKAKLIR 96
P+ S+P +H PP + +STA+ S F ++T R+ +S + +++R
Sbjct: 24 PVPFRPSLPRRSHRHPPPPATNWSSTAKSISRFEAFISTARQFHAERESRFMTRILR 194
>TC9682 similar to UP|Q8VZC0 (Q8VZC0) DTDP-glucose 4-6-dehydratase-like
protein, partial (29%)
Length = 731
Score = 26.9 bits (58), Expect = 4.0
Identities = 21/55 (38%), Positives = 28/55 (50%), Gaps = 3/55 (5%)
Frame = +1
Query: 7 ASSSSSSSSTVGSKFLKPLTAAFSLR--RSISSDSNPITIETSV-PFTAHNCDPP 58
+SSSS SSS S PL+ A +L + S+P+ +TS P TA PP
Sbjct: 337 SSSSSESSSAPPSSSSNPLSPASALTTPHLTPTPSSPLPSQTSTPPQTASAVSPP 501
>BP048961
Length = 543
Score = 26.9 bits (58), Expect = 4.0
Identities = 18/51 (35%), Positives = 24/51 (46%)
Frame = +1
Query: 8 SSSSSSSSTVGSKFLKPLTAAFSLRRSISSDSNPITIETSVPFTAHNCDPP 58
SS SSSSS S PLT + S + ++ T +S+P H PP
Sbjct: 139 SSPSSSSSPSHSPSSLPLTPSSPPPTSAADSTSTSTSPSSLPPPPHPASPP 291
>TC19949 UP|Q8H0D4 (Q8H0D4) Rac GTPase, partial (33%)
Length = 549
Score = 26.6 bits (57), Expect = 5.3
Identities = 20/57 (35%), Positives = 27/57 (47%), Gaps = 6/57 (10%)
Frame = -3
Query: 8 SSSSSSSSTVGSKFLKPLTAAFSLRRSISSDSNPITIET------SVPFTAHNCDPP 58
SSSSSSSS+ S +P ++ L++ N T+ T S P NC PP
Sbjct: 358 SSSSSSSSSSSSSL*EPFYSSSHLKKQKPQIFNLRTLMTHTQTRYSHPVRFPNCAPP 188
>TC19072 homologue to UP|CC37_CAEEL (O02108) Probable Hsp90 co-chaperone
Cdc37 (Hsp90 chaperone protein kinase-targeting
subunit), partial (5%)
Length = 531
Score = 26.2 bits (56), Expect = 6.9
Identities = 13/25 (52%), Positives = 15/25 (60%)
Frame = -3
Query: 4 SRLASSSSSSSSTVGSKFLKPLTAA 28
+R +SSSSSS VG K KP A
Sbjct: 403 NRASSSSSSSCKRVGMKLFKPFKRA 329
>TC18988 similar to UP|FUS_MOUSE (P56959) RNA-binding protein FUS (Pigpen
protein), partial (3%)
Length = 425
Score = 26.2 bits (56), Expect = 6.9
Identities = 16/56 (28%), Positives = 25/56 (44%)
Frame = +3
Query: 2 ALSRLASSSSSSSSTVGSKFLKPLTAAFSLRRSISSDSNPITIETSVPFTAHNCDP 57
+ S A++SS SS +GS + + F + S SNP + S+ C P
Sbjct: 171 SFSAAATASSVSSPAIGSSYQSSPPSTFFPASQVLSLSNPFESKGSIGGLHELCKP 338
>TC12269 homologue to UP|Q8GZT8 (Q8GZT8) PTEN-like protein, partial (20%)
Length = 425
Score = 26.2 bits (56), Expect = 6.9
Identities = 14/38 (36%), Positives = 17/38 (43%)
Frame = -1
Query: 22 LKPLTAAFSLRRSISSDSNPITIETSVPFTAHNCDPPS 59
+KP R S+S NPI I SV + PPS
Sbjct: 176 VKPFKITKKTRGSMSPAGNPIAIMFSVMYVISKSKPPS 63
>TC8133 similar to GB|BAA97157.1|8809606|AB018117 ethylene responsive
element binding factor 5 (ATERF5) {Arabidopsis
thaliana;} , partial (22%)
Length = 785
Score = 25.8 bits (55), Expect = 9.0
Identities = 11/25 (44%), Positives = 16/25 (64%)
Frame = +2
Query: 43 TIETSVPFTAHNCDPPSRSVETSTA 67
T+ SVPFTA NC + S+ + +A
Sbjct: 203 TLMKSVPFTASNCTSSANSLPSRSA 277
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.319 0.134 0.397
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,895,995
Number of Sequences: 28460
Number of extensions: 66786
Number of successful extensions: 564
Number of sequences better than 10.0: 42
Number of HSP's better than 10.0 without gapping: 483
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 542
length of query: 288
length of database: 4,897,600
effective HSP length: 89
effective length of query: 199
effective length of database: 2,364,660
effective search space: 470567340
effective search space used: 470567340
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 55 (25.8 bits)
Lotus: description of TM0028b.3