BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0026.11
(461 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC16653 similar to UP|Q8W1A1 (Q8W1A1) Adenosine 5'-phosphosulfat... 524 0.0
AV409576 239 7e-64
AV413102 198 1e-51
TC14588 similar to UP|AAQ57202 (AAQ57202) Adenosine 5' phosphosu... 151 2e-37
TC13098 homologue to PIR|T10065|T10065 phosphoadenylyl-sulfate r... 56 1e-08
TC8497 similar to UP|PDA6_MEDSA (P38661) Probable protein disulf... 53 1e-07
TC16952 similar to UP|Q84XU4 (Q84XU4) Protein disulphide isomera... 49 2e-06
BP036717 47 5e-06
BP048975 28 0.007
TC19338 similar to GB|AAC49358.1|1388088|PSU35831 thioredoxin m ... 35 0.020
TC14552 similar to UP|THM4_ARATH (Q9SEU6) Thioredoxin M-type 4, ... 35 0.034
AU252164 34 0.058
TC7923 similar to UP|PDI_MEDSA (P29828) Protein disulfide isomer... 34 0.058
BP043842 32 0.22
TC9623 homologue to UP|Q8H1K8 (Q8H1K8) Stromal ascorbate peroxid... 32 0.29
TC10615 similar to UP|Q9LEC9 (Q9LEC9) Alpha-glucosidase , parti... 31 0.49
TC10703 similar to UP|BAD09184 (BAD09184) Eukaryotic translation... 31 0.49
TC15929 similar to UP|THM4_ARATH (Q9SEU6) Thioredoxin M-type 4, ... 31 0.49
CB827734 31 0.49
AU252081 30 1.1
>TC16653 similar to UP|Q8W1A1 (Q8W1A1) Adenosine 5'-phosphosulfate
reductase, partial (69%)
Length = 1242
Score = 524 bits (1350), Expect(2) = 0.0
Identities = 248/248 (100%), Positives = 248/248 (100%)
Frame = +1
Query: 151 FFDEVEKHYGIHIEYMFPDAVEVQGLVRSKGLFSFYEDGHQECCRVRKVRPLRRALKGLR 210
FFDEVEKHYGIHIEYMFPDAVEVQGLVRSKGLFSFYEDGHQECCRVRKVRPLRRALKGLR
Sbjct: 499 FFDEVEKHYGIHIEYMFPDAVEVQGLVRSKGLFSFYEDGHQECCRVRKVRPLRRALKGLR 678
Query: 211 AWITGQRKDQSPGTRSEVPVVQVDPVFEGVDGGVGSLVKWNPVANVKGHDIWSFLRTMNV 270
AWITGQRKDQSPGTRSEVPVVQVDPVFEGVDGGVGSLVKWNPVANVKGHDIWSFLRTMNV
Sbjct: 679 AWITGQRKDQSPGTRSEVPVVQVDPVFEGVDGGVGSLVKWNPVANVKGHDIWSFLRTMNV 858
Query: 271 PVNSLHSKGYISIGCEPCTRAVLPGQHEREGRWWWEDAKAKECGLHKGNVKQDAEAELNG 330
PVNSLHSKGYISIGCEPCTRAVLPGQHEREGRWWWEDAKAKECGLHKGNVKQDAEAELNG
Sbjct: 859 PVNSLHSKGYISIGCEPCTRAVLPGQHEREGRWWWEDAKAKECGLHKGNVKQDAEAELNG 1038
Query: 331 NGVANTNGTATVADIFNTQNVVSLSRTGIENLTKLENRKEPWLVVLYAPWCPYCQAMEES 390
NGVANTNGTATVADIFNTQNVVSLSRTGIENLTKLENRKEPWLVVLYAPWCPYCQAMEES
Sbjct: 1039NGVANTNGTATVADIFNTQNVVSLSRTGIENLTKLENRKEPWLVVLYAPWCPYCQAMEES 1218
Query: 391 YVDLADKL 398
YVDLADKL
Sbjct: 1219YVDLADKL 1242
Score = 293 bits (749), Expect(2) = 0.0
Identities = 152/152 (100%), Positives = 152/152 (100%)
Frame = +3
Query: 1 MALAVTCSISISSSSSSSSTFQSSQPKFSQIASIRVSEIPHGGGVNASQRRSFVKPPQRS 60
MALAVTCSISISSSSSSSSTFQSSQPKFSQIASIRVSEIPHGGGVNASQRRSFVKPPQRS
Sbjct: 48 MALAVTCSISISSSSSSSSTFQSSQPKFSQIASIRVSEIPHGGGVNASQRRSFVKPPQRS 227
Query: 61 RDSIAPLAATIVASDVAETEQDNYQQLAVDLENASPLQIMDAALEKFGNHIAIAFSGAED 120
RDSIAPLAATIVASDVAETEQDNYQQLAVDLENASPLQIMDAALEKFGNHIAIAFSGAED
Sbjct: 228 RDSIAPLAATIVASDVAETEQDNYQQLAVDLENASPLQIMDAALEKFGNHIAIAFSGAED 407
Query: 121 VALIEYARLTGRPFRVFSLDTGRLNPETYRFF 152
VALIEYARLTGRPFRVFSLDTGRLNPETYRFF
Sbjct: 408 VALIEYARLTGRPFRVFSLDTGRLNPETYRFF 503
>AV409576
Length = 418
Score = 239 bits (610), Expect = 7e-64
Identities = 115/136 (84%), Positives = 126/136 (92%)
Frame = +1
Query: 76 VAETEQDNYQQLAVDLENASPLQIMDAALEKFGNHIAIAFSGAEDVALIEYARLTGRPFR 135
V E E+++Y+QLA + +NASPL+IMD ALEKFGN IAIAFSGAEDVALIEYA LTGRP+R
Sbjct: 4 VTEKEEEDYEQLAREFQNASPLEIMDKALEKFGNDIAIAFSGAEDVALIEYAHLTGRPYR 183
Query: 136 VFSLDTGRLNPETYRFFDEVEKHYGIHIEYMFPDAVEVQGLVRSKGLFSFYEDGHQECCR 195
VFSLDTGRLNPETY++FDEVEKHYGI IEYMFPDAVEVQ LVR+KGLFSFYEDGHQECCR
Sbjct: 184 VFSLDTGRLNPETYKYFDEVEKHYGIRIEYMFPDAVEVQALVRAKGLFSFYEDGHQECCR 363
Query: 196 VRKVRPLRRALKGLRA 211
RKVRPLRRALKGLRA
Sbjct: 364 TRKVRPLRRALKGLRA 411
>AV413102
Length = 374
Score = 198 bits (504), Expect = 1e-51
Identities = 96/123 (78%), Positives = 108/123 (87%), Gaps = 2/123 (1%)
Frame = +1
Query: 317 KGNVKQDAEAELNGNG--VANTNGTATVADIFNTQNVVSLSRTGIENLTKLENRKEPWLV 374
+GN++QD +AE NGNG AN NG+ATVADIF+TQNVVSLSRTGIENL KL+NR EPWLV
Sbjct: 1 QGNLRQDGDAEFNGNGNGAANANGSATVADIFDTQNVVSLSRTGIENLAKLKNRNEPWLV 180
Query: 375 VLYAPWCPYCQAMEESYVDLADKLAESGVKVGKFRADGEQKEFAKRELGLGSFPTIMFFP 434
VLYAPWC +CQ MEESY+DLA+KLA SGVKVGKFRADGE KE+AK EL L SFPTI+FFP
Sbjct: 181 VLYAPWCRFCQGMEESYLDLAEKLAGSGVKVGKFRADGEHKEYAKSELELESFPTILFFP 360
Query: 435 KHS 437
KHS
Sbjct: 361 KHS 369
>TC14588 similar to UP|AAQ57202 (AAQ57202) Adenosine 5' phosphosulfate
reductase, partial (16%)
Length = 696
Score = 151 bits (382), Expect = 2e-37
Identities = 75/75 (100%), Positives = 75/75 (100%)
Frame = +3
Query: 387 MEESYVDLADKLAESGVKVGKFRADGEQKEFAKRELGLGSFPTIMFFPKHSSRPIKYPSE 446
MEESYVDLADKLAESGVKVGKFRADGEQKEFAKRELGLGSFPTIMFFPKHSSRPIKYPSE
Sbjct: 3 MEESYVDLADKLAESGVKVGKFRADGEQKEFAKRELGLGSFPTIMFFPKHSSRPIKYPSE 182
Query: 447 NRDVDSLMAFVNALR 461
NRDVDSLMAFVNALR
Sbjct: 183 NRDVDSLMAFVNALR 227
>TC13098 homologue to PIR|T10065|T10065 phosphoadenylyl-sulfate reductase
(thioredoxin) precursor, chloroplast -
Madagascar periwinkle {Catharanthus roseus;} , partial
(6%)
Length = 537
Score = 56.2 bits (134), Expect = 1e-08
Identities = 26/28 (92%), Positives = 26/28 (92%)
Frame = +2
Query: 434 PKHSSRPIKYPSENRDVDSLMAFVNALR 461
PKHSSRPIKYPSE RDVDSLMAFVN LR
Sbjct: 2 PKHSSRPIKYPSEKRDVDSLMAFVNILR 85
>TC8497 similar to UP|PDA6_MEDSA (P38661) Probable protein disulfide
isomerase A6 precursor (P5) , partial (95%)
Length = 1533
Score = 52.8 bits (125), Expect = 1e-07
Identities = 39/125 (31%), Positives = 59/125 (47%), Gaps = 1/125 (0%)
Frame = +2
Query: 335 NTNGTATVADIFNTQNVVSLSRTGIENLTKLENRKEPWLVVLYAPWCPYCQAMEESYVDL 394
NT G V +VV L+ + L+ K+ LV YAPWC +C+++ +Y +
Sbjct: 503 NTEGGTNVKIATAPSSVVVLTAENFNEVV-LDETKDV-LVEFYAPWCGHCKSLAPTYEKV 676
Query: 395 ADKLAESG-VKVGKFRADGEQKEFAKRELGLGSFPTIMFFPKHSSRPIKYPSENRDVDSL 453
A G V + AD + K E + FPT+ FFPK + +Y RD+D
Sbjct: 677 AAAFKLDGDVVIANLDADKYRDLAEKYE--VSGFPTLKFFPKGNKAGEEY-GGGRDLDDF 847
Query: 454 MAFVN 458
+AF+N
Sbjct: 848 VAFIN 862
Score = 52.4 bits (124), Expect = 2e-07
Identities = 35/113 (30%), Positives = 54/113 (46%), Gaps = 1/113 (0%)
Frame = +2
Query: 347 NTQNVVSLSRTGIENLTKLENRKEPWLVVLYAPWCPYCQAMEESYVDLADKLAES-GVKV 405
+ +VV LS +N K + + LV YAPWC +C+ + Y L ++ V +
Sbjct: 185 SADDVVVLSE---DNFEKEVGQDKGALVEFYAPWCGHCKKLAPEYEKLGGSFKKAKSVLI 355
Query: 406 GKFRADGEQKEFAKRELGLGSFPTIMFFPKHSSRPIKYPSENRDVDSLMAFVN 458
K D + +K G+ +PT+ +FPK S P KY R ++L FVN
Sbjct: 356 AKVDCDEHKSVCSK--YGVSGYPTLQWFPKGSLEPKKYEGP-RTAEALAEFVN 505
>TC16952 similar to UP|Q84XU4 (Q84XU4) Protein disulphide isomerase
(Fragment), partial (44%)
Length = 921
Score = 48.5 bits (114), Expect = 2e-06
Identities = 29/72 (40%), Positives = 39/72 (53%), Gaps = 3/72 (4%)
Frame = +2
Query: 373 LVVLYAPWCPYCQAMEESYVDLADKLAE-SGVKVGKFRADGEQKEFAKRELGLGSFPTIM 431
L+ +YAPWC +CQA+E +Y LA L + + + K + AK E FPTI+
Sbjct: 275 LLEIYAPWCGHCQALEPTYNKLAKHLHDIESIVIAKMDGTTNEHPRAKAE----GFPTIL 442
Query: 432 FFP--KHSSRPI 441
FFP SS PI
Sbjct: 443 FFPAGSKSSDPI 478
>BP036717
Length = 477
Score = 47.4 bits (111), Expect = 5e-06
Identities = 24/73 (32%), Positives = 34/73 (45%)
Frame = +1
Query: 361 NLTKLENRKEPWLVVLYAPWCPYCQAMEESYVDLADKLAESGVKVGKFRADGEQKEFAKR 420
N T + +V YAPWC +CQ++ Y A +L GV + K D Q+
Sbjct: 172 NFTTVVENNRFVMVEFYAPWCGHCQSLAPEYAAAATELKNDGVVLAK--VDATQENDLAH 345
Query: 421 ELGLGSFPTIMFF 433
E + FPT+ FF
Sbjct: 346 EYDVQGFPTVSFF 384
>BP048975
Length = 530
Score = 28.1 bits (61), Expect(2) = 0.007
Identities = 11/15 (73%), Positives = 12/15 (79%)
Frame = -3
Query: 208 GLRAWITGQRKDQSP 222
GL +WI GQR DQSP
Sbjct: 246 GLTSWIIGQRMDQSP 202
Score = 27.7 bits (60), Expect(2) = 0.007
Identities = 9/13 (69%), Positives = 11/13 (84%)
Frame = -2
Query: 183 FSFYEDGHQECCR 195
F + EDGHQECC+
Sbjct: 319 FFYEEDGHQECCK 281
>TC19338 similar to GB|AAC49358.1|1388088|PSU35831 thioredoxin m {Pisum
sativum;} , partial (65%)
Length = 476
Score = 35.4 bits (80), Expect = 0.020
Identities = 20/64 (31%), Positives = 33/64 (51%), Gaps = 1/64 (1%)
Frame = +2
Query: 371 PWLVVLYAPWCPYCQAMEESYVDLADKLA-ESGVKVGKFRADGEQKEFAKRELGLGSFPT 429
P LV +APWC C+ + L D+LA E K+ ++ + + + G+ S PT
Sbjct: 86 PVLVDFWAPWCGPCRMI----APLIDELAKEYAGKIACYKLNTDDSPNIATQYGIRSIPT 253
Query: 430 IMFF 433
++FF
Sbjct: 254 VLFF 265
>TC14552 similar to UP|THM4_ARATH (Q9SEU6) Thioredoxin M-type 4, chloroplast
precursor (TRX-M4), partial (55%)
Length = 1016
Score = 34.7 bits (78), Expect = 0.034
Identities = 28/101 (27%), Positives = 49/101 (47%), Gaps = 4/101 (3%)
Frame = +1
Query: 337 NGTATVADIFNTQN----VVSLSRTGIENLTKLENRKEPWLVVLYAPWCPYCQAMEESYV 392
N TA V + Q+ V S++ ++L LE+ + P LV +APWC C+ +
Sbjct: 247 NRTARVGVVCEAQDTAVEVASITDANWQSLV-LES-EFPVLVEFWAPWCGPCRMIHPIID 420
Query: 393 DLADKLAESGVKVGKFRADGEQKEFAKRELGLGSFPTIMFF 433
+LA + A K+ ++ + ++ G+ S PT+M F
Sbjct: 421 ELAKQYAG---KLKCYKLNTDESPSTATRYGIRSIPTVMIF 534
>AU252164
Length = 350
Score = 33.9 bits (76), Expect = 0.058
Identities = 17/71 (23%), Positives = 33/71 (45%), Gaps = 1/71 (1%)
Frame = +2
Query: 364 KLENRKEPWLVVLYAPWCPYCQAMEESYVDLADKL-AESGVKVGKFRADGEQKEFAKREL 422
K++ + W V PWC +C+ + + DL + E + +G+ E+ +K +
Sbjct: 134 KIKEKDTAWFVKFCVPWCKHCKNLGSLWDDLGTAMEGEDEIXIGEVDCSTEKPVCSK--V 307
Query: 423 GLGSFPTIMFF 433
+ S+PT F
Sbjct: 308 DIHSYPTFKVF 340
>TC7923 similar to UP|PDI_MEDSA (P29828) Protein disulfide isomerase
precursor (PDI) , partial (89%)
Length = 1861
Score = 33.9 bits (76), Expect = 0.058
Identities = 21/99 (21%), Positives = 45/99 (45%), Gaps = 2/99 (2%)
Frame = +2
Query: 361 NLTKLENRKEPWLVVLYAPWCPYCQAMEESYVDLADKLA--ESGVKVGKFRADGEQKEFA 418
N +++ ++ + +V YAPWC +C+ + Y A L+ + + + K A+ E +
Sbjct: 170 NFSEIVSKHDFIVVEFYAPWCGHCKNLAPEYEKAAAVLSSHDPPIVLAKVDANEENNKDL 349
Query: 419 KRELGLGSFPTIMFFPKHSSRPIKYPSENRDVDSLMAFV 457
+ + FPTI +Y R+ D ++ ++
Sbjct: 350 ASQYEVRGFPTIKILRNGGKDSQEYKGP-READGIVDYL 463
Score = 29.6 bits (65), Expect = 1.1
Identities = 20/90 (22%), Positives = 39/90 (43%), Gaps = 1/90 (1%)
Frame = +2
Query: 373 LVVLYAPWCPYCQAMEESYVDLADKLA-ESGVKVGKFRADGEQKEFAKRELGLGSFPTIM 431
L+ YAPWC +C+++ ++A ++ + + KF D + + +PT+
Sbjct: 1241 LLEFYAPWCGHCKSLAPILDEVAVSYQNDADIVIAKF--DATANDVPTESFEVQGYPTLY 1414
Query: 432 FFPKHSSRPIKYPSENRDVDSLMAFVNALR 461
F SS I R + ++ F+ R
Sbjct: 1415 FI--SSSGKISPYDGGRTKEDIIQFIENSR 1498
>BP043842
Length = 490
Score = 32.0 bits (71), Expect = 0.22
Identities = 12/17 (70%), Positives = 12/17 (70%)
Frame = -2
Query: 289 TRAVLPGQHEREGRWWW 305
TRAV EREGRWWW
Sbjct: 114 TRAVSDQPEEREGRWWW 64
>TC9623 homologue to UP|Q8H1K8 (Q8H1K8) Stromal ascorbate peroxidase,
partial (42%)
Length = 640
Score = 31.6 bits (70), Expect = 0.29
Identities = 24/64 (37%), Positives = 33/64 (51%), Gaps = 8/64 (12%)
Frame = +3
Query: 8 SISISSSSSSSSTFQ--SSQPKFSQI------ASIRVSEIPHGGGVNASQRRSFVKPPQR 59
S S SSSSSS S+FQ S P+ S + A +RVS GG S +SF P +
Sbjct: 156 SFSSSSSSSSRSSFQCLRSSPRISHLFLNQGRAEVRVSS---GGFATVSAPKSFASDPDQ 326
Query: 60 SRDS 63
+++
Sbjct: 327 LKNA 338
>TC10615 similar to UP|Q9LEC9 (Q9LEC9) Alpha-glucosidase , partial (13%)
Length = 548
Score = 30.8 bits (68), Expect = 0.49
Identities = 19/56 (33%), Positives = 30/56 (52%), Gaps = 1/56 (1%)
Frame = +2
Query: 1 MALAVTCSISISSSSSSSSTFQSSQPKFSQ-IASIRVSEIPHGGGVNASQRRSFVK 55
+ L + +++ SSSSSSSS+ S+ K Q + I + E P GG + Q + K
Sbjct: 65 LTLFLCAAVATSSSSSSSSSSSSNATKIGQGYSLISIEETPDGGLIGLLQVKKKTK 232
>TC10703 similar to UP|BAD09184 (BAD09184) Eukaryotic translation initiation
factor 3 subunit (EIF-3)-like, partial (16%)
Length = 1066
Score = 30.8 bits (68), Expect = 0.49
Identities = 25/90 (27%), Positives = 36/90 (39%)
Frame = +1
Query: 12 SSSSSSSSTFQSSQPKFSQIASIRVSEIPHGGGVNASQRRSFVKPPQRSRDSIAPLAATI 71
+S + S+ T QSS K S I S++P+GG + S D ++P
Sbjct: 109 NSQTRSAGTLQSSSDKASHIVLHGDSQVPNGGKNDTSSTEDL--NGTEVTDDVSP----- 267
Query: 72 VASDVAETEQDNYQQLAVDLENASPLQIMD 101
+AE EQ Y DL+ Q D
Sbjct: 268 -EGQLAENEQATYASANNDLKGTKAYQEAD 354
>TC15929 similar to UP|THM4_ARATH (Q9SEU6) Thioredoxin M-type 4, chloroplast
precursor (TRX-M4), partial (49%)
Length = 1406
Score = 30.8 bits (68), Expect = 0.49
Identities = 16/61 (26%), Positives = 30/61 (48%)
Frame = +1
Query: 373 LVVLYAPWCPYCQAMEESYVDLADKLAESGVKVGKFRADGEQKEFAKRELGLGSFPTIMF 432
LV +APWC C+ + +LA + A K+ ++ + ++ G+ S PT++
Sbjct: 364 LVEFWAPWCGPCRMIHPVIDELAKEYAG---KLKCYKLNTDESPSTATRYGIRSIPTVII 534
Query: 433 F 433
F
Sbjct: 535 F 537
>CB827734
Length = 556
Score = 30.8 bits (68), Expect = 0.49
Identities = 20/65 (30%), Positives = 32/65 (48%)
Frame = +1
Query: 363 TKLENRKEPWLVVLYAPWCPYCQAMEESYVDLADKLAESGVKVGKFRADGEQKEFAKREL 422
+K+ N K LV +APWC +C+A+ + + A + + V V AD Q +E
Sbjct: 166 SKVLNSKGVVLVEFFAPWCGHCKALTPIW-EKAATVLKGVVTVAALDADAHQA--LAQEY 336
Query: 423 GLGSF 427
G+ F
Sbjct: 337 GIRGF 351
>AU252081
Length = 296
Score = 29.6 bits (65), Expect = 1.1
Identities = 10/23 (43%), Positives = 13/23 (56%)
Frame = +2
Query: 285 CEPCTRAVLPGQHEREGRWWWED 307
C P V G +REGR WW++
Sbjct: 59 CRPSQGQVFSGSEQREGRSWWKE 127
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.317 0.134 0.399
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,712,984
Number of Sequences: 28460
Number of extensions: 107357
Number of successful extensions: 1471
Number of sequences better than 10.0: 60
Number of HSP's better than 10.0 without gapping: 1129
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1299
length of query: 461
length of database: 4,897,600
effective HSP length: 93
effective length of query: 368
effective length of database: 2,250,820
effective search space: 828301760
effective search space used: 828301760
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 57 (26.6 bits)
Lotus: description of TM0026.11