BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0023.4
(236 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP042379 32 0.074
TC8198 similar to UP|P93273 (P93273) PAFD103 protein (Fragment),... 32 0.074
TC8558 weakly similar to UP|Q9NDI0 (Q9NDI0) 200 kDa antigen p200... 30 0.28
BU494432 30 0.37
TC8858 homologue to UP|Q84N37 (Q84N37) Potyvirus VPg interacting... 30 0.48
TC7822 similar to UP|Q42139 (Q42139) H+-transporting ATP synthas... 29 0.81
BP055954 29 0.81
TC12467 UP|CAE02597 (CAE02597) Nod-factor receptor 5, complete 28 1.4
BP048961 27 2.4
BP064704 27 3.1
TC7812 UP|Q9LKJ6 (Q9LKJ6) Water-selective transport intrinsic me... 27 3.1
BP050993 27 3.1
CN825477 27 4.0
BP038960 27 4.0
AU252214 27 4.0
TC16733 weakly similar to UP|Q8W106 (Q8W106) AT3g27930/K24A2_2, ... 27 4.0
AU251952 26 5.3
BP042574 26 6.9
TC9443 similar to UP|Q84N37 (Q84N37) Potyvirus VPg interacting p... 26 6.9
BP068044 26 6.9
>BP042379
Length = 560
Score = 32.3 bits (72), Expect = 0.074
Identities = 30/142 (21%), Positives = 62/142 (43%), Gaps = 8/142 (5%)
Frame = -2
Query: 81 KGVRKKSPLVDEREAK-RQKSDASKGTLAVNQALAAASCATGLLPHLKGVEIDKACAQLK 139
+ + K S ++D+ E + + + +A+ +A A+ A ++ H + +
Sbjct: 472 QSILKWSQIIDQMEDQIAEVLQEEREEIALRKAEMEAAKAENMIAHREEIFSRPKRTWFV 293
Query: 140 LEEAKAAYSRLKSDAENAAK--RAEIAKKQAEDTLAGVRKQLQEADEVLAKDK-----AA 192
E+ K ++ D+ + K R E+ Q + L K+ +E ++ L + K AA
Sbjct: 292 TEKEKKLVAKAAKDSMDTGKSSRKEVISAQEAEDLKMKEKRKREREKNLPRKKRRKLEAA 113
Query: 193 RESAEASDEEKEEEAKGEGVKK 214
RE E + E+ +G+G K
Sbjct: 112 REMLEDDEGYDEKPVEGKGANK 47
>TC8198 similar to UP|P93273 (P93273) PAFD103 protein (Fragment), partial
(65%)
Length = 850
Score = 32.3 bits (72), Expect = 0.074
Identities = 23/68 (33%), Positives = 36/68 (52%), Gaps = 8/68 (11%)
Frame = +3
Query: 176 RKQLQEADEVLAK-DKAARESAEASDE-------EKEEEAKGEGVKKADVKVGGSGEVNQ 227
+K+L E + +LA A +ES + DE +K +EA G+G KK GGS N
Sbjct: 90 KKELAELEALLADFGVAPKESTDGQDETQGAPQDKKGDEADGDGEKKETTAAGGS--KNA 263
Query: 228 EEQAKNDQ 235
+++ K D+
Sbjct: 264 KKKKKKDK 287
>TC8558 weakly similar to UP|Q9NDI0 (Q9NDI0) 200 kDa antigen p200
(Fragment), partial (4%)
Length = 585
Score = 30.4 bits (67), Expect = 0.28
Identities = 23/91 (25%), Positives = 42/91 (45%), Gaps = 2/91 (2%)
Frame = +1
Query: 141 EEAKAAYSRLKSDAENAAKRAEIAKKQAEDTLAGVRK-QLQEADEVLAKDKAARESAEAS 199
EE+ + DAE + ++Q A + Q +E +++ ARE+AEA
Sbjct: 1 EESSPTLVAARLDAEERRNNIRLREEQDAAYRAALEADQARERQRREEQERLAREAAEAE 180
Query: 200 DEEK-EEEAKGEGVKKADVKVGGSGEVNQEE 229
+ K EEEA+ ++A K ++ Q++
Sbjct: 181 RKRKEEEEAREREAREAAEKQAALAKIRQQK 273
>BU494432
Length = 538
Score = 30.0 bits (66), Expect = 0.37
Identities = 31/104 (29%), Positives = 41/104 (38%), Gaps = 7/104 (6%)
Frame = +1
Query: 3 CFKPPKIRNFQIFSVSFNSVSRLKSS-PTIFLFTALYSVF--CAAEMVKNKG----GVNP 55
C P R +F + FN VS LKS L TA ++ C K V P
Sbjct: 106 CLSAPLRR--ALFGLPFNDVSSLKSKVQRNKLATAGEAIEYRCLIRATDGKKTISTSVGP 279
Query: 56 ESMSAFIASQVETAKGHRKNEKKETKGVRKKSPLVDEREAKRQK 99
+ F AS K H KK + +KKS D+RE ++
Sbjct: 280 KDHQRFQASYATILKAHMTALKKRERKDKKKSAEADKREGSSKR 411
>TC8858 homologue to UP|Q84N37 (Q84N37) Potyvirus VPg interacting protein
(Fragment), partial (65%)
Length = 1272
Score = 29.6 bits (65), Expect = 0.48
Identities = 27/104 (25%), Positives = 48/104 (45%)
Frame = +1
Query: 113 LAAASCATGLLPHLKGVEIDKACAQLKLEEAKAAYSRLKSDAENAAKRAEIAKKQAEDTL 172
L + + +L + +E+D + LE A+ RL + E+ + AE+ ++
Sbjct: 358 LESKAACRAILVVFQELEVD---SPKTLENAEGG--RLIAPQESCNRIAEVVQE------ 504
Query: 173 AGVRKQLQEADEVLAKDKAARESAEASDEEKEEEAKGEGVKKAD 216
+RK ADE + K AR + EA D E ++A+ G K +
Sbjct: 505 -AIRKMEMVADEKMRMFKKARLALEACDRELADKAREAGELKTE 633
>TC7822 similar to UP|Q42139 (Q42139) H+-transporting ATP synthase CHAIN9 -
like protein (AT4G32260/F10M6_100) , partial (76%)
Length = 907
Score = 28.9 bits (63), Expect = 0.81
Identities = 35/113 (30%), Positives = 54/113 (46%), Gaps = 11/113 (9%)
Frame = +3
Query: 87 SPL---VDEREAK-RQKSDASKGTLAVNQALAAASCATGLLPHLKGV--EIDKACAQLKL 140
SPL +D+R+A R+K ++ K T + + L + A LK EI A ++K
Sbjct: 414 SPLGKFMDDRDAAIREKLNSVKDTSSEVKQLEEQANAV-----LKAARAEISAALNKMKK 578
Query: 141 E-----EAKAAYSRLKSDAENAAKRAEIAKKQAEDTLAGVRKQLQEADEVLAK 188
E E K A RLK DAE A + +KQ E+T+ + Q+ + + K
Sbjct: 579 ETQVEVEQKIAEGRLKVDAELQEALANL-EKQKEETIKSLDSQIAALSQDIVK 734
>BP055954
Length = 518
Score = 28.9 bits (63), Expect = 0.81
Identities = 19/45 (42%), Positives = 26/45 (57%), Gaps = 3/45 (6%)
Frame = -3
Query: 182 ADEVLAKDKAARESAEASDEEKEEEAKGEGVKKADVKVG---GSG 223
A V+A+ AA E E+EEEA+G+G A+ +VG GSG
Sbjct: 144 AAAVVAESTAAEVGREDEG*EEEEEAEGDGD*VAEAEVGEVRGSG 10
>TC12467 UP|CAE02597 (CAE02597) Nod-factor receptor 5, complete
Length = 2292
Score = 28.1 bits (61), Expect = 1.4
Identities = 20/79 (25%), Positives = 30/79 (37%), Gaps = 1/79 (1%)
Frame = +3
Query: 147 YSRLKSDAENAAKRAEIAKKQAEDTLAGVRK-QLQEADEVLAKDKAARESAEASDEEKEE 205
Y R K A AE A K V K + E DE++ K + + + +
Sbjct: 948 YCRRKKALNRTASSAETADKLLSGVSGYVSKPNVYEIDEIMEATKDFSDECKVGESVYKA 1127
Query: 206 EAKGEGVKKADVKVGGSGE 224
+G V +K GG+ E
Sbjct: 1128 NIEGRVVAVKKIKEGGANE 1184
>BP048961
Length = 543
Score = 27.3 bits (59), Expect = 2.4
Identities = 17/56 (30%), Positives = 29/56 (51%)
Frame = -2
Query: 175 VRKQLQEADEVLAKDKAARESAEASDEEKEEEAKGEGVKKADVKVGGSGEVNQEEQ 230
V +++ A EV ++ R S E + +EEE EG + +V SGE +E++
Sbjct: 245 VEVEVESAAEVGGGEEGVRGSDEGECDGEEEEEGEEGEEGP*WRVWESGEEEEEDE 78
>BP064704
Length = 471
Score = 26.9 bits (58), Expect = 3.1
Identities = 15/54 (27%), Positives = 26/54 (47%)
Frame = +2
Query: 48 KNKGGVNPESMSAFIASQVETAKGHRKNEKKETKGVRKKSPLVDEREAKRQKSD 101
+N GG +SMS ++ +T K TKG +K+ ++ + +A SD
Sbjct: 251 ENSGG---DSMSLSVSGSKDTTNLEANRGKTTTKGKKKRREILQKADAAGSTSD 403
>TC7812 UP|Q9LKJ6 (Q9LKJ6) Water-selective transport intrinsic membrane
protein 1, complete
Length = 1149
Score = 26.9 bits (58), Expect = 3.1
Identities = 15/42 (35%), Positives = 24/42 (56%), Gaps = 1/42 (2%)
Frame = -2
Query: 194 ESAEASDEEKEEEAKGE-GVKKADVKVGGSGEVNQEEQAKND 234
++AE D +EEA+G+ GV A VG G+ ++E + D
Sbjct: 386 DTAEEGDVTADEEAEGDGGVDVAAGDVGADGDGDEEGECVGD 261
>BP050993
Length = 436
Score = 26.9 bits (58), Expect = 3.1
Identities = 11/39 (28%), Positives = 22/39 (56%)
Frame = -1
Query: 4 FKPPKIRNFQIFSVSFNSVSRLKSSPTIFLFTALYSVFC 42
F PP + ++ + SV + + ++ S T FLF + ++ C
Sbjct: 217 FTPPVMSSYGVLSVEDSGICKIISCITFFLFFSHFNRLC 101
>CN825477
Length = 466
Score = 26.6 bits (57), Expect = 4.0
Identities = 13/29 (44%), Positives = 17/29 (57%)
Frame = -1
Query: 194 ESAEASDEEKEEEAKGEGVKKADVKVGGS 222
E+AEA E+EEE EG AD + G+
Sbjct: 217 EAAEAESTEEEEERTAEGSPVADTLLEGN 131
>BP038960
Length = 524
Score = 26.6 bits (57), Expect = 4.0
Identities = 18/53 (33%), Positives = 25/53 (46%), Gaps = 2/53 (3%)
Frame = +3
Query: 174 GVRKQLQ--EADEVLAKDKAARESAEASDEEKEEEAKGEGVKKADVKVGGSGE 224
G QLQ EA++ +D + DEE+EEE + E V V G G+
Sbjct: 255 GEHTQLQKGEAEQPRVEDSEEEAADYDDDEEEEEEEEEEDEHADRVAVSGPGK 413
>AU252214
Length = 350
Score = 26.6 bits (57), Expect = 4.0
Identities = 21/75 (28%), Positives = 31/75 (41%)
Frame = +1
Query: 45 EMVKNKGGVNPESMSAFIASQVETAKGHRKNEKKETKGVRKKSPLVDEREAKRQKSDASK 104
E + K GVN + A + K ++EKK + K+ E E R ++ K
Sbjct: 64 EKMPKKMGVNSK------AEEARARKASAESEKKNKETKEKEDQFWKEAEGSRSRAAKKK 225
Query: 105 GTLAVNQALAAASCA 119
A +A AAA A
Sbjct: 226 EEEAEKKAEAAAKRA 270
>TC16733 weakly similar to UP|Q8W106 (Q8W106) AT3g27930/K24A2_2, partial
(26%)
Length = 629
Score = 26.6 bits (57), Expect = 4.0
Identities = 18/51 (35%), Positives = 23/51 (44%), Gaps = 2/51 (3%)
Frame = -2
Query: 71 GHRKNEKKETKGV--RKKSPLVDEREAKRQKSDASKGTLAVNQALAAASCA 119
G RKNE K+ + R L KSD S G V QA+ +A C+
Sbjct: 364 GKRKNEAKKFHKILSRGLKSLGKPSNCPASKSDCSIGRCPVVQAMPSAKCS 212
>AU251952
Length = 323
Score = 26.2 bits (56), Expect = 5.3
Identities = 15/57 (26%), Positives = 30/57 (52%), Gaps = 6/57 (10%)
Frame = -1
Query: 55 PESMSAFIASQVETAKGHRKNEKKE------TKGVRKKSPLVDEREAKRQKSDASKG 105
PE+M++ +T K H+K +KK+ TK +++ ++D++ + S KG
Sbjct: 227 PETMTSLTHENKKTTKNHKKKKKKKKLQNRLTK-QKEEGSVLDQKGRDKSSSTNHKG 60
>BP042574
Length = 505
Score = 25.8 bits (55), Expect = 6.9
Identities = 16/56 (28%), Positives = 30/56 (53%)
Frame = +3
Query: 130 EIDKACAQLKLEEAKAAYSRLKSDAENAAKRAEIAKKQAEDTLAGVRKQLQEADEV 185
EI+ ++L E+KAA ++ + E+ AK +++ + TL G + ADE+
Sbjct: 303 EIEDLVSELLEVESKAAEAQEALEEESLAKVESEVRQELKQTLQGDDLETAVADEM 470
>TC9443 similar to UP|Q84N37 (Q84N37) Potyvirus VPg interacting protein
(Fragment), partial (41%)
Length = 1025
Score = 25.8 bits (55), Expect = 6.9
Identities = 20/69 (28%), Positives = 28/69 (39%)
Frame = +1
Query: 168 AEDTLAGVRKQLQEADEVLAKDKAARESAEASDEEKEEEAKGEGVKKADVKVGGSGEVNQ 227
AE +RK ADE + K AR + +A D E ++A+ E+
Sbjct: 112 AEVVQEAIRKMEMVADEKMRMFKKARLALDACDRELADKAR------------EVAELKM 255
Query: 228 EEQAKNDQV 236
E Q K QV
Sbjct: 256 ERQKKKSQV 282
>BP068044
Length = 266
Score = 25.8 bits (55), Expect = 6.9
Identities = 12/32 (37%), Positives = 17/32 (52%)
Frame = +1
Query: 70 KGHRKNEKKETKGVRKKSPLVDEREAKRQKSD 101
KG + EKK +G +K + D KRQK +
Sbjct: 64 KGQ*RTEKKFEQGTKKTAIATDPXHRKRQKKE 159
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.307 0.123 0.319
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,672,352
Number of Sequences: 28460
Number of extensions: 25091
Number of successful extensions: 200
Number of sequences better than 10.0: 44
Number of HSP's better than 10.0 without gapping: 197
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 198
length of query: 236
length of database: 4,897,600
effective HSP length: 87
effective length of query: 149
effective length of database: 2,421,580
effective search space: 360815420
effective search space used: 360815420
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.6 bits)
S2: 54 (25.4 bits)
Lotus: description of TM0023.4