BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0022.20
(96 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP056513 33 0.006
TC14869 33 0.006
BP031757 32 0.018
BP085186 32 0.018
BI417283 30 0.069
TC15307 30 0.090
TC14095 homologue to UP|RS2_ARATH (P49688) 40S ribosomal protein... 30 0.090
AW720070 30 0.090
TC16666 GB|AAH26381.1|20071724|BC026381 Ddx27 protein {Mus muscu... 29 0.12
BP043925 28 0.34
TC9200 similar to UP|Q8LLE6 (Q8LLE6) RNA-binding protein AKIP1, ... 27 0.45
TC10380 similar to UP|Q8S8U2 (Q8S8U2) Ycf1 protein, partial (40%) 27 0.45
TC8572 similar to UP|Q9SCM3 (Q9SCM3) 40S ribosomal protein S2 ho... 27 0.45
AU251865 27 0.59
AV775527 27 0.77
TC18370 27 0.77
AW428931 27 0.77
TC10607 26 1.00
TC9853 26 1.00
TC12561 UP|Q9FAM6 (Q9FAM6) DNA gyrase subunit B (Fragment) , pa... 26 1.00
>BP056513
Length = 451
Score = 33.5 bits (75), Expect = 0.006
Identities = 18/34 (52%), Positives = 24/34 (69%)
Frame = +2
Query: 3 RRRTEEEDSKKKKKRGRGGRRRTQPYHESKKKEK 36
RRR EEED ++KKK+ R RRRT+ S+ +EK
Sbjct: 311 RRRREEEDERRKKKKKR-FRRRTEKKTNSEGEEK 409
Score = 33.1 bits (74), Expect = 0.008
Identities = 15/43 (34%), Positives = 29/43 (66%)
Frame = +3
Query: 2 GRRRTEEEDSKKKKKRGRGGRRRTQPYHESKKKEKMRLKKRNR 44
G+R ++ E+ +++KR RGGRRR + E ++K + + +KR +
Sbjct: 291 GQRGSDREE--EERKRTRGGRRRRRDSEEEQRKRRTQKEKRRK 413
Score = 26.2 bits (56), Expect = 1.00
Identities = 13/34 (38%), Positives = 19/34 (55%), Gaps = 1/34 (2%)
Frame = +1
Query: 13 KKKKRGRGGRRRTQPYH-ESKKKEKMRLKKRNRE 45
+KKKRGRG + + K +EK L++R E
Sbjct: 310 EKKKRGRGREEEEEEEEIQKKNREKDELRRRREE 411
>TC14869
Length = 828
Score = 33.5 bits (75), Expect = 0.006
Identities = 18/41 (43%), Positives = 25/41 (60%)
Frame = -1
Query: 10 DSKKKKKRGRGGRRRTQPYHESKKKEKMRLKKRNREVLRFI 50
+ KKKKK+GRG R R + E KKK+ ++ RE RF+
Sbjct: 228 EKKKKKKQGRGLRNRVEE-GEKKKKKPWLQRREERERERFV 109
Score = 24.6 bits (52), Expect = 2.9
Identities = 14/40 (35%), Positives = 23/40 (57%), Gaps = 2/40 (5%)
Frame = -3
Query: 1 MGRRRT--EEEDSKKKKKRGRGGRRRTQPYHESKKKEKMR 38
+GRRR EEE+ + +++ + RRR + E+ EK R
Sbjct: 250 IGRRREIGEEEEEETRERIEKQSRRRREEEEEAMVAEKGR 131
>BP031757
Length = 507
Score = 32.0 bits (71), Expect = 0.018
Identities = 16/45 (35%), Positives = 24/45 (52%)
Frame = +3
Query: 4 RRTEEEDSKKKKKRGRGGRRRTQPYHESKKKEKMRLKKRNREVLR 48
R+T + ++ +R R RRR P K+K+K + RNR LR
Sbjct: 165 RKTTKTKMERITRRKRRRRRRKSPGRRRKRKKKKNKRNRNRRSLR 299
>BP085186
Length = 388
Score = 32.0 bits (71), Expect = 0.018
Identities = 13/45 (28%), Positives = 26/45 (56%)
Frame = -2
Query: 1 MGRRRTEEEDSKKKKKRGRGGRRRTQPYHESKKKEKMRLKKRNRE 45
MG+ +++ KKKKK+ + +++ Q + + K KM K+ +E
Sbjct: 273 MGKEMKKKKKKKKKKKKKKKKKKKKQNQRKKRMKRKMSTPKKEKE 139
>BI417283
Length = 557
Score = 30.0 bits (66), Expect = 0.069
Identities = 12/28 (42%), Positives = 19/28 (67%)
Frame = -1
Query: 18 GRGGRRRTQPYHESKKKEKMRLKKRNRE 45
GRGGRRR + Y E +K++K + ++ E
Sbjct: 125 GRGGRRRRRCYQEQEKEQKWKKEETEEE 42
Score = 27.7 bits (60), Expect = 0.34
Identities = 12/29 (41%), Positives = 18/29 (61%)
Frame = -2
Query: 7 EEEDSKKKKKRGRGGRRRTQPYHESKKKE 35
EEE + + K++ + GRRR Q +E KE
Sbjct: 109 EEEGATRSKRKSKSGRRRKQKKNERGGKE 23
>TC15307
Length = 589
Score = 29.6 bits (65), Expect = 0.090
Identities = 18/55 (32%), Positives = 29/55 (52%)
Frame = -3
Query: 19 RGGRRRTQPYHESKKKEKMRLKKRNREVLRFIEQFLHSSSTNLQNIYITTYPFKI 73
+G R TQ + K+KEK++ K +NR VL+ + + L N +PFK+
Sbjct: 470 KGTRGATQGTQKEKRKEKLQTKVKNRRVLQKQRKQGKTKWDQL*N*SPQKHPFKL 306
>TC14095 homologue to UP|RS2_ARATH (P49688) 40S ribosomal protein S2,
partial (88%)
Length = 1134
Score = 29.6 bits (65), Expect = 0.090
Identities = 23/80 (28%), Positives = 37/80 (45%)
Frame = +3
Query: 17 RGRGGRRRTQPYHESKKKEKMRLKKRNREVLRFIEQFLHSSSTNLQNIYITTYPFKITQV 76
RGRGGRRRT + ++K + + K R V +L+ IY+ + P K Q+
Sbjct: 132 RGRGGRRRTGGRRDEEEK-WVPVTKLGRLV-------RDGKIRSLEQIYLHSLPIKEHQI 287
Query: 77 MKKKVKPSLASGACTLSSLQ 96
+ V P+L + +Q
Sbjct: 288 IDMLVGPTLKDEVMKIMPVQ 347
>AW720070
Length = 484
Score = 29.6 bits (65), Expect = 0.090
Identities = 18/64 (28%), Positives = 34/64 (53%)
Frame = +2
Query: 7 EEEDSKKKKKRGRGGRRRTQPYHESKKKEKMRLKKRNREVLRFIEQFLHSSSTNLQNIYI 66
E++ KKKKKR +++ Q ES ++++ + KK+ + + FLH L ++Y+
Sbjct: 107 EKKRKKKKKKR----KKKKQVKKESSQQQEKKKKKKKKAAVSTTAPFLHP----LISLYL 262
Query: 67 TTYP 70
P
Sbjct: 263 LLLP 274
>TC16666 GB|AAH26381.1|20071724|BC026381 Ddx27 protein {Mus musculus;} ,
partial (6%)
Length = 582
Score = 29.3 bits (64), Expect = 0.12
Identities = 17/60 (28%), Positives = 33/60 (54%)
Frame = +2
Query: 5 RTEEEDSKKKKKRGRGGRRRTQPYHESKKKEKMRLKKRNREVLRFIEQFLHSSSTNLQNI 64
+TE +D +K +K G G ++ + +SK + K +K + +L + F+H + NLQ +
Sbjct: 5 QTEIKD-RKPRKGGSGAGKKPKSSFKSKSRYK---RK*SMRLLNLVITFMHGRTINLQQV 172
>BP043925
Length = 450
Score = 27.7 bits (60), Expect = 0.34
Identities = 16/50 (32%), Positives = 29/50 (58%), Gaps = 2/50 (4%)
Frame = -1
Query: 49 FIEQFLHSSSTNLQNIYI--TTYPFKITQVMKKKVKPSLASGACTLSSLQ 96
FI FL+ S ++L +++ +TY F I + ++ V GA TLS+++
Sbjct: 156 FISHFLYLSHSSLISLFFFPSTYQFNILFISQRIVLKGGTRGAPTLSTIE 7
>TC9200 similar to UP|Q8LLE6 (Q8LLE6) RNA-binding protein AKIP1, partial
(31%)
Length = 727
Score = 27.3 bits (59), Expect = 0.45
Identities = 13/37 (35%), Positives = 22/37 (59%)
Frame = +2
Query: 12 KKKKKRGRGGRRRTQPYHESKKKEKMRLKKRNREVLR 48
++KKKR + RR + + KKK K + K+R + + R
Sbjct: 233 RRKKKRRKRKRRVKKKKKKKKKKRKWKRKRRRKTMNR 343
Score = 26.9 bits (58), Expect = 0.59
Identities = 13/40 (32%), Positives = 24/40 (59%)
Frame = +2
Query: 3 RRRTEEEDSKKKKKRGRGGRRRTQPYHESKKKEKMRLKKR 42
R ++ ++ KK +K+ R ++R + KKK+K + KKR
Sbjct: 185 RFKSTKKSKKK*RKKWRRKKKRRKRKRRVKKKKKKKKKKR 304
Score = 24.6 bits (52), Expect = 2.9
Identities = 11/30 (36%), Positives = 19/30 (62%)
Frame = +2
Query: 3 RRRTEEEDSKKKKKRGRGGRRRTQPYHESK 32
+RR +++ KKKKKR +RR + + S+
Sbjct: 260 KRRVKKKKKKKKKKRKWKRKRRRKTMNRSR 349
>TC10380 similar to UP|Q8S8U2 (Q8S8U2) Ycf1 protein, partial (40%)
Length = 5664
Score = 27.3 bits (59), Expect = 0.45
Identities = 14/39 (35%), Positives = 22/39 (55%)
Frame = +2
Query: 13 KKKKRGRGGRRRTQPYHESKKKEKMRLKKRNREVLRFIE 51
+KKK + + ES+KKE+ ++KK +E R IE
Sbjct: 2192 RKKKELKNSDYTDEKAKESQKKEEEKIKKNEKEEKRRIE 2308
>TC8572 similar to UP|Q9SCM3 (Q9SCM3) 40S ribosomal protein S2 homolog
(At3g57490), partial (91%)
Length = 1076
Score = 27.3 bits (59), Expect = 0.45
Identities = 22/80 (27%), Positives = 36/80 (44%)
Frame = +2
Query: 17 RGRGGRRRTQPYHESKKKEKMRLKKRNREVLRFIEQFLHSSSTNLQNIYITTYPFKITQV 76
RGRGGRRR + ++K + + K R V +L+ IY+ + P K Q+
Sbjct: 152 RGRGGRRRGAGRRDEEEK-WVPVTKLGRLV-------RDGKIRSLEQIYLHSLPIKEHQI 307
Query: 77 MKKKVKPSLASGACTLSSLQ 96
+ V P+L + +Q
Sbjct: 308 IDTLVGPTLKDEVMKIMPVQ 367
>AU251865
Length = 161
Score = 26.9 bits (58), Expect = 0.59
Identities = 17/43 (39%), Positives = 25/43 (57%)
Frame = +3
Query: 5 RTEEEDSKKKKKRGRGGRRRTQPYHESKKKEKMRLKKRNREVL 47
+ EEE+ +K++KR + G RR+Q YH + L REVL
Sbjct: 9 KEEEEEVRKRRKRKKHG-RRSQ*YHHRRNSSSNCL---YREVL 125
>AV775527
Length = 466
Score = 26.6 bits (57), Expect = 0.77
Identities = 13/43 (30%), Positives = 24/43 (55%), Gaps = 3/43 (6%)
Frame = -2
Query: 2 GRRRTEEEDSKKKKKRGRG---GRRRTQPYHESKKKEKMRLKK 41
G RR+ + ++ +RGRG GRRR + K + ++R ++
Sbjct: 411 GSRRSRYRGTSRRIRRGRGGG*GRRRRRGKQRMKTQRRLRWRR 283
>TC18370
Length = 397
Score = 26.6 bits (57), Expect = 0.77
Identities = 13/36 (36%), Positives = 23/36 (63%), Gaps = 2/36 (5%)
Frame = +3
Query: 3 RRRTEE--EDSKKKKKRGRGGRRRTQPYHESKKKEK 36
RRRT E +++++ R RRRT+P E+ ++E+
Sbjct: 237 RRRTRPL*EATRRERSESRHHRRRTRPL*EATRRER 344
>AW428931
Length = 284
Score = 26.6 bits (57), Expect = 0.77
Identities = 11/20 (55%), Positives = 16/20 (80%)
Frame = +3
Query: 4 RRTEEEDSKKKKKRGRGGRR 23
RRT++ +SK KKK+GR R+
Sbjct: 222 RRTQQGNSKIKKKKGRESRK 281
>TC10607
Length = 411
Score = 26.2 bits (56), Expect = 1.00
Identities = 12/21 (57%), Positives = 14/21 (66%)
Frame = -3
Query: 4 RRTEEEDSKKKKKRGRGGRRR 24
R EE KKKKK+G+ RRR
Sbjct: 70 RVIREEKKKKKKKKGKWWRRR 8
>TC9853
Length = 551
Score = 26.2 bits (56), Expect = 1.00
Identities = 10/25 (40%), Positives = 17/25 (68%)
Frame = -2
Query: 55 HSSSTNLQNIYITTYPFKITQVMKK 79
HSS LQ +Y T+ PF++ Q +++
Sbjct: 505 HSSYLYLQKVYQTSVPFQVWQTLQE 431
>TC12561 UP|Q9FAM6 (Q9FAM6) DNA gyrase subunit B (Fragment) , partial (6%)
Length = 566
Score = 26.2 bits (56), Expect = 1.00
Identities = 16/47 (34%), Positives = 26/47 (55%), Gaps = 2/47 (4%)
Frame = -2
Query: 5 RTEEEDSKKKKKR--GRGGRRRTQPYHESKKKEKMRLKKRNREVLRF 49
R +++ +KK+K RGGR P + + + +R K RNR +RF
Sbjct: 472 RNKDKQRRKKEKAVIARGGRGLRNP-KQWRLRRTLRAKLRNRVRIRF 335
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.315 0.129 0.349
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,541,492
Number of Sequences: 28460
Number of extensions: 18528
Number of successful extensions: 395
Number of sequences better than 10.0: 112
Number of HSP's better than 10.0 without gapping: 350
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 381
length of query: 96
length of database: 4,897,600
effective HSP length: 72
effective length of query: 24
effective length of database: 2,848,480
effective search space: 68363520
effective search space used: 68363520
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 47 (22.7 bits)
Lotus: description of TM0022.20