BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0021.5
(210 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP043003 80 2e-16
TC17762 70 4e-13
AV769205 69 6e-13
TC19130 similar to UP|Q7YSZ8 (Q7YSZ8) GE-rich protein precursor,... 69 8e-13
TC8030 66 5e-12
AV780927 62 6e-11
BP050007 61 1e-10
TC8028 similar to GB|AAA30143.1|552290|TOXNTP nucleoside triphos... 61 1e-10
TC8866 61 2e-10
TC13202 60 2e-10
AV416076 59 8e-10
AV407514 57 2e-09
AV777838 55 9e-09
TC12746 weakly similar to UP|Q8RWQ0 (Q8RWQ0) AT5g38990/K15E6_170... 54 2e-08
BP039163 52 6e-08
AV428786 42 7e-08
TC8026 49 8e-07
AV419705 47 2e-06
TC18252 45 9e-06
BP065844 45 9e-06
>BP043003
Length = 493
Score = 80.5 bits (197), Expect = 2e-16
Identities = 55/120 (45%), Positives = 60/120 (49%), Gaps = 1/120 (0%)
Frame = -2
Query: 92 EPLTIWRNQHHHCTPLTVRNKTPQEIFRFAALRRRASALQLAISPSFGLRWPRNHHHHAL 151
EPLTIWRNQH HCTPLTVRNKTPQEI
Sbjct: 465 EPLTIWRNQHRHCTPLTVRNKTPQEI---------------------------------- 388
Query: 152 HGERNKTLYSFSSPATLPPLAAVLRHLLQPISPEKSAELVLKIQAFWVGFV-DFRVKGLI 210
LYS SSPAT PPLAAV RH L P PEKS + + ++G+V F VKGLI
Sbjct: 387 -------LYSSSSPATSPPLAAVRRHPLWPALPEKSG-----LVSNFLGWVCCF*VKGLI 244
>TC17762
Length = 463
Score = 69.7 bits (169), Expect = 4e-13
Identities = 43/83 (51%), Positives = 47/83 (55%), Gaps = 3/83 (3%)
Frame = +1
Query: 47 PPPRTLLLPFPLFQPRAPLPPCLLHE--AMKPPRNPSRRYLQSPISGEPLTIWRNQHHHC 104
PPP L +P A P HE A P R+L SPISGEPL+ WRN+H
Sbjct: 196 PPPLPLRSLWPAASTIASSSPSDGHERAAKATTTKP*NRHLSSPISGEPLSFWRNRHRRW 375
Query: 105 TPLTVRNKTPQEIFRFAA-LRRR 126
TPL VRNKTPQ I FAA LRRR
Sbjct: 376 TPLDVRNKTPQSI*GFAAELRRR 444
Score = 29.3 bits (64), Expect = 0.52
Identities = 35/132 (26%), Positives = 47/132 (35%), Gaps = 21/132 (15%)
Frame = +3
Query: 44 RKPPPPRTLLLPFPLFQPRAPLPPCLLHEAMKPPRNPSRRYLQSPISGEPLTIWRNQHHH 103
R P P R P PL R PPCLLH PP + + P ++ HH
Sbjct: 90 RHPRPSRHHREPLPL--TRMLPPPCLLHANTPPP-------WATTAASPPSFSLASREHH 242
Query: 104 CTPLTVR----------NKTPQEIFRFAALRRRASALQLAI-----------SPSFGLRW 142
+T+R +KT + F LRR L+ +P LR+
Sbjct: 243 RVFITIRRP*TRRQSHHHKTLKPPSFFPDLRRTTEFLEKPTPSLDSP*CPQQNPPINLRF 422
Query: 143 PRNHHHHALHGE 154
R A+ GE
Sbjct: 423 RRRAPPEAIPGE 458
>AV769205
Length = 600
Score = 68.9 bits (167), Expect = 6e-13
Identities = 41/80 (51%), Positives = 46/80 (57%), Gaps = 6/80 (7%)
Frame = +3
Query: 44 RKPPPPRTLLLPFPLFQPRAPLPPCLLHEA------MKPPRNPSRRYLQSPISGEPLTIW 97
R+P R+L + PL C E M+PP NPSR +LQSPISGEPL W
Sbjct: 219 RQPRHHRSLCFVDQSREQPPPL*ACFRREKEV*GRKMEPP*NPSR-HLQSPISGEPLIYW 395
Query: 98 RNQHHHCTPLTVRNKTPQEI 117
RN HH TPL VRNKTPQ I
Sbjct: 396 RN*HHRWTPLIVRNKTPQSI 455
>TC19130 similar to UP|Q7YSZ8 (Q7YSZ8) GE-rich protein precursor, partial
(20%)
Length = 603
Score = 68.6 bits (166), Expect = 8e-13
Identities = 36/65 (55%), Positives = 42/65 (64%), Gaps = 5/65 (7%)
Frame = +2
Query: 58 LFQPRAPLPPCLLHEAMK-----PPRNPSRRYLQSPISGEPLTIWRNQHHHCTPLTVRNK 112
L QPRAP P H M PP+NPSR +L PISG+PL+ WRN+HH TPL +RNK
Sbjct: 221 LLQPRAPPRPHHHHVTMNEPPWSPPQNPSR-HLFPPISGDPLSFWRNKHHQ*TPLIIRNK 397
Query: 113 TPQEI 117
TP I
Sbjct: 398 TPPSI 412
>TC8030
Length = 991
Score = 65.9 bits (159), Expect = 5e-12
Identities = 41/91 (45%), Positives = 49/91 (53%), Gaps = 9/91 (9%)
Frame = +1
Query: 44 RKPPPPRTLLLPFPLFQPRAPLPPCLLHEAMKPP---------RNPSRRYLQSPISGEPL 94
R+ P R+L +P AP P HE P R P R+L +PISGEPL
Sbjct: 220 RRLSPFRSL---WPAASTTAPSSPSNDHERPTPATTDAKNKEKRKP*IRHLSTPISGEPL 390
Query: 95 TIWRNQHHHCTPLTVRNKTPQEIFRFAALRR 125
+ W+NQHHH TPL VR KTP+EI LRR
Sbjct: 391 SFWKNQHHHGTPLDVRFKTPKEISDSDHLRR 483
Score = 35.0 bits (79), Expect = 0.010
Identities = 25/73 (34%), Positives = 32/73 (43%)
Frame = +2
Query: 43 TRKPPPPRTLLLPFPLFQPRAPLPPCLLHEAMKPPRNPSRRYLQSPISGEPLTIWRNQHH 102
+R P P R P PL R PPC+LH PP + +SG+P R HH
Sbjct: 110 SRHPGPSRHHREPLPL--TRMLPPPCILHRNAPPPWATTVASPPFVLSGQPRAPPR-LHH 280
Query: 103 HCTPLTVRNKTPQ 115
H T + + PQ
Sbjct: 281 HQTTMNAPPQPPQ 319
>AV780927
Length = 496
Score = 62.4 bits (150), Expect = 6e-11
Identities = 33/74 (44%), Positives = 45/74 (60%)
Frame = -3
Query: 70 LHEAMKPPRNPSRRYLQSPISGEPLTIWRNQHHHCTPLTVRNKTPQEIFRFAALRRRASA 129
LH+ ++ R P R+L SPISGEPL+ WRNQHH TPL +R +TP+EI LRR+
Sbjct: 446 LHQ-IEEKRKP*NRHLSSPISGEPLSFWRNQHHG*TPLIIRFQTPKEILDPDHLRRKPPP 270
Query: 130 LQLAISPSFGLRWP 143
+ +F +P
Sbjct: 269 TTVFSRETFAGEFP 228
>BP050007
Length = 567
Score = 61.2 bits (147), Expect = 1e-10
Identities = 33/66 (50%), Positives = 40/66 (60%), Gaps = 1/66 (1%)
Frame = +1
Query: 62 RAPLPPCLLHEA-MKPPRNPSRRYLQSPISGEPLTIWRNQHHHCTPLTVRNKTPQEIFRF 120
R+P P +++A + P R+LQ ISGEPL WRN HH TPLT RNKTPQ I
Sbjct: 118 RSPRPHPRVNDATIATTEKP*HRHLQPSISGEPLIYWRN*HHRWTPLTFRNKTPQSICHS 297
Query: 121 AALRRR 126
LRR+
Sbjct: 298 DNLRRK 315
>TC8028 similar to GB|AAA30143.1|552290|TOXNTP nucleoside triphosphate
hydrolase {Toxoplasma gondii;} , partial (8%)
Length = 1114
Score = 61.2 bits (147), Expect = 1e-10
Identities = 57/159 (35%), Positives = 66/159 (40%), Gaps = 37/159 (23%)
Frame = +3
Query: 4 FFTFLFMCLILEIVQTMKNKISTAVVLTRCYRDDTRKAATRKPPPPRTLLLP-------- 55
FF+FL +V+ KN + ++L + KPPPPR P
Sbjct: 6 FFSFL-------LVKQQKNTTTITILLLLPW----------KPPPPRAFSSPHASQSTTT 134
Query: 56 ----------------FPLFQPRA-----------PLPPCLLHEAMKPPR--NPSRRYLQ 86
PL Q RA P PP KP R NPSR +L
Sbjct: 135 FSLTNQHHQAIIFLFSLPLVQIRAHHQRPTP*TTPPQPPW----RRKPERK*NPSR-HLP 299
Query: 87 SPISGEPLTIWRNQHHHCTPLTVRNKTPQEIFRFAALRR 125
SPISGEPL+ WR Q HH TPL VR KTP EI LRR
Sbjct: 300 SPISGEPLSFWRKQPHHGTPLDVRCKTP*EISDSDNLRR 416
Score = 27.7 bits (60), Expect = 1.5
Identities = 30/114 (26%), Positives = 39/114 (33%)
Frame = +1
Query: 46 PPPPRTLLLPFPLFQPRAPLPPCLLHEAMKPPRNPSRRYLQSPISGEPLTIWRNQHHHCT 105
PPP R P P LL A PP +PSR P S +W H
Sbjct: 43 PPPSPYFFFSHGSRHHREPSP--LLTRARAPPPSPSRTSTIKPSSSSFPCLWFRYEH--- 207
Query: 106 PLTVRNKTPQEIFRFAALRRRASALQLAISPSFGLRWPRNHHHHALHGERNKTL 159
N E R + ++ +F +R P N H + GE N T+
Sbjct: 208 ---TTNAQHHERRRHSHHGGENQRENETLAATFQVRSPAN---H*VFGESNHTM 351
>TC8866
Length = 754
Score = 60.8 bits (146), Expect = 2e-10
Identities = 27/42 (64%), Positives = 33/42 (78%)
Frame = +1
Query: 76 PPRNPSRRYLQSPISGEPLTIWRNQHHHCTPLTVRNKTPQEI 117
PP+NPSR +L PISG+PL+ WRN+HH TPL +RNKTP I
Sbjct: 40 PPQNPSR-HLFPPISGDPLSFWRNKHHQ*TPLIIRNKTPPSI 162
>TC13202
Length = 431
Score = 60.5 bits (145), Expect = 2e-10
Identities = 26/38 (68%), Positives = 30/38 (78%)
Frame = +3
Query: 78 RNPSRRYLQSPISGEPLTIWRNQHHHCTPLTVRNKTPQ 115
RNP R+L +PISGEPL+ WRNQ HH TPL VR KTP+
Sbjct: 318 RNPKNRHLSTPISGEPLSFWRNQPHHGTPLDVRCKTPK 431
>AV416076
Length = 430
Score = 58.5 bits (140), Expect = 8e-10
Identities = 31/64 (48%), Positives = 39/64 (60%), Gaps = 5/64 (7%)
Frame = +3
Query: 59 FQPRAPLP-PCLLHEAMKP----PRNPSRRYLQSPISGEPLTIWRNQHHHCTPLTVRNKT 113
+QP+ P P P +++A P R+L SPISGEPL+ WRN+H TPL VR KT
Sbjct: 234 WQPQPPSPTPSTMNDATNARTIVKTKP*NRHLSSPISGEPLSFWRNRHRCWTPLDVRKKT 413
Query: 114 PQEI 117
PQ I
Sbjct: 414 PQSI 425
>AV407514
Length = 329
Score = 57.4 bits (137), Expect = 2e-09
Identities = 25/34 (73%), Positives = 29/34 (84%)
Frame = +2
Query: 84 YLQSPISGEPLTIWRNQHHHCTPLTVRNKTPQEI 117
+LQSPISGEPL+ W+NQ HH TPL VR KTP+EI
Sbjct: 227 HLQSPISGEPLSFWKNQPHHGTPLAVRCKTPKEI 328
>AV777838
Length = 565
Score = 55.1 bits (131), Expect = 9e-09
Identities = 26/35 (74%), Positives = 27/35 (76%)
Frame = +2
Query: 83 RYLQSPISGEPLTIWRNQHHHCTPLTVRNKTPQEI 117
R LQSPI GEPLTI NQHH C PL VRNKT +EI
Sbjct: 449 RRLQSPIYGEPLTISTNQHHRCAPLLVRNKTHKEI 553
>TC12746 weakly similar to UP|Q8RWQ0 (Q8RWQ0) AT5g38990/K15E6_170, partial
(3%)
Length = 558
Score = 53.9 bits (128), Expect = 2e-08
Identities = 49/119 (41%), Positives = 58/119 (48%), Gaps = 7/119 (5%)
Frame = -1
Query: 62 RAPLPPCLLHE----AMKPPRNPSRRYLQSPISGEPLTIWRNQHHHCTPLTVRNKTPQEI 117
++P PP LL A P + R L SPI +P TI RN HHH L VR KT +
Sbjct: 378 QSPSPPDLLLTSTTLASSPLMHG*NRDLLSPIPAKPQTI*RNHHHHRISLAVRRKTHKSD 199
Query: 118 FRFAALRRRASALQLAISPSFGLRWPRNH---HHHALHGERNKTLYSFSSPATLPPLAA 173
R AA+RR L +A + F LR + GE TL SSPATLPP AA
Sbjct: 198 RRSAAVRRHRHHL*MAFTG*F-LRLLKKRTPPR*PPPRGEEVPTL--SSSPATLPPCAA 31
>BP039163
Length = 544
Score = 52.4 bits (124), Expect = 6e-08
Identities = 27/49 (55%), Positives = 32/49 (65%)
Frame = -1
Query: 90 SGEPLTIWRNQHHHCTPLTVRNKTPQEIFRFAALRRRASALQLAISPSF 138
SGEPL+ WRN+HHH TPL +RNKTP I R +A A+A A S F
Sbjct: 544 SGEPLSFWRNKHHH*TPLIIRNKTPPSI-R*SATVTPATAAAAAFSGEF 401
>AV428786
Length = 432
Score = 42.4 bits (98), Expect(2) = 7e-08
Identities = 19/30 (63%), Positives = 19/30 (63%)
Frame = +2
Query: 88 PISGEPLTIWRNQHHHCTPLTVRNKTPQEI 117
PI EPLT WRNQ HH T RNKTP I
Sbjct: 206 PIFREPLTYWRNQRHH*TLFIYRNKTPSSI 295
Score = 29.3 bits (64), Expect(2) = 7e-08
Identities = 11/14 (78%), Positives = 12/14 (85%)
Frame = +1
Query: 60 QPRAPLPPCLLHEA 73
+PRAP PPCLLH A
Sbjct: 91 KPRAPPPPCLLHSA 132
>TC8026
Length = 667
Score = 48.5 bits (114), Expect = 8e-07
Identities = 25/57 (43%), Positives = 32/57 (55%)
Frame = +1
Query: 87 SPISGEPLTIWRNQHHHCTPLTVRNKTPQEIFRFAALRRRASALQLAISPSFGLRWP 143
SPISGEPL+ W HH TPL +R KTP+EI LRR+ + +F +P
Sbjct: 1 SPISGEPLSFWSYLHHG*TPLIIRFKTPKEILDSDHLRRKPPPTTVFSRETFAGEFP 171
>AV419705
Length = 395
Score = 47.0 bits (110), Expect = 2e-06
Identities = 25/57 (43%), Positives = 29/57 (50%)
Frame = +3
Query: 61 PRAPLPPCLLHEAMKPPRNPSRRYLQSPISGEPLTIWRNQHHHCTPLTVRNKTPQEI 117
PR PP H + P R+L PISGE L + NQHH TPL KTP+ I
Sbjct: 222 PRTRRPPPREHAEINTTMKP*NRHLSPPISGELLIVLGNQHHD*TPLEASFKTPKPI 392
>TC18252
Length = 627
Score = 45.1 bits (105), Expect = 9e-06
Identities = 23/47 (48%), Positives = 30/47 (62%), Gaps = 3/47 (6%)
Frame = +1
Query: 95 TIWRNQHHHCTPLTVRNKTPQEIFRFAALRRR---ASALQLAISPSF 138
+ WRNQHHH TPLTVR KTP+EI + L + A+A + P+F
Sbjct: 1 SFWRNQHHH*TPLTVRYKTPKEILQSDNLHWKPPLAAANHRLLRPTF 141
>BP065844
Length = 403
Score = 45.1 bits (105), Expect = 9e-06
Identities = 23/50 (46%), Positives = 30/50 (60%), Gaps = 5/50 (10%)
Frame = -3
Query: 75 KPPRN-----PSRRYLQSPISGEPLTIWRNQHHHCTPLTVRNKTPQEIFR 119
KP RN P R+LQ+PI G+PL+ WRN +H T L + +TP I R
Sbjct: 356 KPQRN*GETQP*NRHLQAPIFGQPLSFWRNPYHR*TTLIICFQTPPSICR 207
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.326 0.139 0.444
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,353,356
Number of Sequences: 28460
Number of extensions: 109195
Number of successful extensions: 2142
Number of sequences better than 10.0: 239
Number of HSP's better than 10.0 without gapping: 1822
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2077
length of query: 210
length of database: 4,897,600
effective HSP length: 86
effective length of query: 124
effective length of database: 2,450,040
effective search space: 303804960
effective search space used: 303804960
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 53 (25.0 bits)
Lotus: description of TM0021.5