BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0018.4
(408 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP065719 108 2e-24
TC14766 similar to UP|Q7XXR8 (Q7XXR8) Nascent polypeptide associ... 36 0.013
CB826779 34 0.039
TC9022 similar to PIR|T46225|T46225 alpha NAC-like protein - Ara... 33 0.086
TC16848 homologue to UP|BAD08451 (BAD08451) Glucose-6-phosphate ... 32 0.19
TC18706 similar to UP|BAD11339 (BAD11339) BRI1-KD interacting pr... 30 0.56
TC8445 similar to UP|O80910 (O80910) At2g38410 protein, partial ... 29 1.2
BP045748 29 1.6
CB829224 27 4.7
AV408212 27 4.7
TC17614 homologue to UP|O04792 (O04792) Acyl-ACP thioesterase, p... 27 6.2
TC17597 similar to UP|AZF1_YEAST (P41696) Asparagine-rich zinc f... 27 8.1
AV406876 27 8.1
TC15077 homologue to UP|RS8_PRUAR (O81361) 40S ribosomal protein... 27 8.1
TC8606 similar to UP|AAQ82688 (AAQ82688) Epa4p, partial (7%) 27 8.1
>BP065719
Length = 567
Score = 108 bits (269), Expect = 2e-24
Identities = 53/76 (69%), Positives = 63/76 (82%)
Frame = +3
Query: 180 NRNQNDYGGPQNVANAVEEVLNHHGFNVGYVNRPNFTSPFSEVVLQAELPRGGKVPKVTK 239
+R+QN GG QNVA VE++LN HGFNVG+ NRP+F S F E VL++ELPRG KVPK TK
Sbjct: 21 DRHQNA-GGQQNVAAVVEQLLNQHGFNVGFANRPHFVSAFIEEVLESELPRGWKVPKFTK 197
Query: 240 FSGDTGESTVEHIARH 255
FSGD+GESTVEHIAR+
Sbjct: 198 FSGDSGESTVEHIARY 245
Score = 31.2 bits (69), Expect = 0.33
Identities = 13/25 (52%), Positives = 18/25 (72%)
Frame = +3
Query: 254 RHMETRCSTHVPEFELVKMAIGGLD 278
R +++RC THV E ELV + GGL+
Sbjct: 492 RMLKSRCFTHVSEHELVVLXAGGLE 566
>TC14766 similar to UP|Q7XXR8 (Q7XXR8) Nascent polypeptide associated
complex alpha chain, partial (58%)
Length = 600
Score = 35.8 bits (81), Expect = 0.013
Identities = 20/64 (31%), Positives = 32/64 (49%)
Frame = +2
Query: 335 QDEVDQSDEDNDSDENEVNMAEFKPKSPYTYEGENKRKIPAKSFRGLKTTSDYQRVMVKR 394
+D+ D+ D+D D D E + +S T + RK K G+K + RV VK+
Sbjct: 149 EDDDDEDDDDEDDDNIEGQEGDASGRSKQTRSEKKSRKAMLKL--GMKPVTGVSRVTVKK 322
Query: 395 SQNL 398
S+N+
Sbjct: 323 SKNI 334
>CB826779
Length = 469
Score = 34.3 bits (77), Expect = 0.039
Identities = 21/74 (28%), Positives = 40/74 (53%), Gaps = 5/74 (6%)
Frame = +1
Query: 330 NSEIGQDEVDQSDEDNDSDENEVNMAEFKPKSPYTYEGENKRKIPAKSFR-----GLKTT 384
++ + +D D++DED D DE++ + + + T EG + + KS + GLK
Sbjct: 190 DAPVVEDVHDEADEDEDDDEDD---EDDEDGAQGTTEGSKQSRSEKKSRKAMLKLGLKPV 360
Query: 385 SDYQRVMVKRSQNL 398
+ RV +KR++N+
Sbjct: 361 TGVSRVTIKRTKNI 402
>TC9022 similar to PIR|T46225|T46225 alpha NAC-like protein - Arabidopsis
thaliana {Arabidopsis thaliana;}, partial (75%)
Length = 1083
Score = 33.1 bits (74), Expect = 0.086
Identities = 22/118 (18%), Positives = 54/118 (45%)
Frame = +3
Query: 281 IKKNLVNDEFIDMIQLADKVRRVEKLRLEKYKPEEVFQGEESSCIRATQNSEIGQDEVDQ 340
+ +N+ +D A+ + +V K + Q +++ + ++ + + + D+
Sbjct: 87 VSQNMAPGPVVDASSEAEALEQVLSAEETTLKKKPQPQEDDAPIVEDVKDDDKDEADDDE 266
Query: 341 SDEDNDSDENEVNMAEFKPKSPYTYEGENKRKIPAKSFRGLKTTSDYQRVMVKRSQNL 398
++D+D D+++ + A + E K + A GLK + RV +KR++N+
Sbjct: 267 DEDDDDEDDDKEDGALGGSEGSKQSRSEKKSR-KAMLKLGLKPVTGVSRVTIKRTKNI 437
>TC16848 homologue to UP|BAD08451 (BAD08451) Glucose-6-phosphate isomerase,
partial (7%)
Length = 723
Score = 32.0 bits (71), Expect = 0.19
Identities = 16/35 (45%), Positives = 22/35 (62%)
Frame = -1
Query: 273 AIGGLDYSIKKNLVNDEFIDMIQLADKVRRVEKLR 307
A+ GL+YSI L+N DM QLA+ R +E L+
Sbjct: 723 AVNGLNYSI**KLIN*PLRDMTQLAENFRSIEHLK 619
>TC18706 similar to UP|BAD11339 (BAD11339) BRI1-KD interacting protein 111
(Fragment), partial (16%)
Length = 640
Score = 30.4 bits (67), Expect = 0.56
Identities = 22/75 (29%), Positives = 37/75 (49%), Gaps = 2/75 (2%)
Frame = +3
Query: 325 IRATQNSEIGQD-EVDQSDEDNDSDENEVNMAEFKPKSPYTYEGENKRKIPAKSFRG-LK 382
I A Q+ + G D EV+ SD+DND +V+ A + + + + KRK KS
Sbjct: 3 IDALQSGDEGSDDEVEGSDDDNDIQIGDVDDASDEEEEDQVSKRKRKRKSGGKSAASPFA 182
Query: 383 TTSDYQRVMVKRSQN 397
+ +++ +M QN
Sbjct: 183 SYEEFEHLMDNVDQN 227
>TC8445 similar to UP|O80910 (O80910) At2g38410 protein, partial (7%)
Length = 841
Score = 29.3 bits (64), Expect = 1.2
Identities = 21/79 (26%), Positives = 32/79 (39%)
Frame = +2
Query: 126 SFTQHEVQNFSQNEPQNLSQDQNFGPNEVLNGQENSVEKQPHNFDQNLDNNVLVNRNQND 185
S T + + S N + + N PN N+ HN N +N+ NRN
Sbjct: 92 SLTPNHSYSLSPNPNTFSNLNHNLSPNP------NTFSNLNHNLSSNTFSNLNHNRNFR- 250
Query: 186 YGGPQNVANAVEEVLNHHG 204
N+ + +E +L HHG
Sbjct: 251 ----INIFSTLEHILPHHG 295
>BP045748
Length = 595
Score = 28.9 bits (63), Expect = 1.6
Identities = 29/88 (32%), Positives = 38/88 (42%)
Frame = +1
Query: 29 FNTLGMTMPLIQSGSLNLFPSNSTTDIDLQSIRQQIDESRYNMVSMLTQQMSTMLNPLIQ 88
F+ T P QS N+ P +ST I S++ S T++ PL+
Sbjct: 235 FSLYKSTNPTTQSHQPNMAPQSST*----------ISSSQF---SHHHHHPRTVIVPLVM 375
Query: 89 NTQHLTRQLCRIADVFGAPPLNQAFHQV 116
T HLTRQL R A V LN FH +
Sbjct: 376 VTVHLTRQLWRSALV---ELLNTHFHVI 450
>CB829224
Length = 582
Score = 27.3 bits (59), Expect = 4.7
Identities = 30/119 (25%), Positives = 47/119 (39%)
Frame = +2
Query: 264 VPEFELVKMAIGGLDYSIKKNLVNDEFIDMIQLADKVRRVEKLRLEKYKPEEVFQGEESS 323
VP+ IGG+ YS+ K VN E ++ ++ D V + ++P +QG
Sbjct: 8 VPKVLEFYSGIGGMRYSLVKAQVNAEVVEAFEINDTANDVYQHNF-AHRP---YQG-NIQ 172
Query: 324 CIRATQNSEIGQDEVDQSDEDNDSDENEVNMAEFKPKSPYTYEGENKRKIPAKSFRGLK 382
C+ A + G D S P PYT +G K A++F L+
Sbjct: 173 CLTAADLDKYGADAWLLS----------------PPCQPYTRQGLQKDTGDARAFSFLQ 301
>AV408212
Length = 362
Score = 27.3 bits (59), Expect = 4.7
Identities = 20/82 (24%), Positives = 32/82 (38%)
Frame = +3
Query: 115 QVPDYGQSVPLSFTQHEVQNFSQNEPQNLSQDQNFGPNEVLNGQENSVEKQPHNFDQNLD 174
Q + Q + +H Q + Q Q Q Q+ S ++QP + Q+ D
Sbjct: 33 QQQQHMQMQQILLQRHAALQQQQQQQQQQQQQQQ---------QQQSQQQQPQSQQQSRD 185
Query: 175 NNVLVNRNQNDYGGPQNVANAV 196
L+N + N G ANA+
Sbjct: 186 RAHLLNGSANGLVGNSGTANAL 251
>TC17614 homologue to UP|O04792 (O04792) Acyl-ACP thioesterase, partial
(23%)
Length = 554
Score = 26.9 bits (58), Expect = 6.2
Identities = 13/32 (40%), Positives = 21/32 (65%)
Frame = -2
Query: 193 ANAVEEVLNHHGFNVGYVNRPNFTSPFSEVVL 224
+N ++E+ N GFN G + PNF +P E++L
Sbjct: 466 SNLLQEISN--GFNSGSLIDPNFIAPHYELLL 377
>TC17597 similar to UP|AZF1_YEAST (P41696) Asparagine-rich zinc finger
protein AZF1, partial (3%)
Length = 399
Score = 26.6 bits (57), Expect = 8.1
Identities = 15/67 (22%), Positives = 30/67 (44%)
Frame = +1
Query: 170 DQNLDNNVLVNRNQNDYGGPQNVANAVEEVLNHHGFNVGYVNRPNFTSPFSEVVLQAELP 229
++ +++N N+NQ+ P N + N++ +N N +S S + Q ++P
Sbjct: 163 NREINSNQNQNQNQSSSSSPMNSPSPRSNASNNNNYN----NNNCMSSSNSNSISQIQIP 330
Query: 230 RGGKVPK 236
PK
Sbjct: 331 TSPHTPK 351
>AV406876
Length = 234
Score = 26.6 bits (57), Expect = 8.1
Identities = 13/35 (37%), Positives = 17/35 (48%)
Frame = -1
Query: 133 QNFSQNEPQNLSQDQNFGPNEVLNGQENSVEKQPH 167
Q SQ+ PQN QN P L+ Q + + PH
Sbjct: 123 QQTSQHLPQNHHTHQNHHPQNPLSPQNSPPHRAPH 19
>TC15077 homologue to UP|RS8_PRUAR (O81361) 40S ribosomal protein S8,
partial (98%)
Length = 987
Score = 26.6 bits (57), Expect = 8.1
Identities = 14/31 (45%), Positives = 21/31 (67%)
Frame = -1
Query: 29 FNTLGMTMPLIQSGSLNLFPSNSTTDIDLQS 59
FNT+ + +PL++ S+NL +NST D L S
Sbjct: 444 FNTIVLEVPLLEWSSINL--NNSTFDKGLSS 358
>TC8606 similar to UP|AAQ82688 (AAQ82688) Epa4p, partial (7%)
Length = 1008
Score = 26.6 bits (57), Expect = 8.1
Identities = 9/23 (39%), Positives = 14/23 (60%)
Frame = -2
Query: 329 QNSEIGQDEVDQSDEDNDSDENE 351
+ E G+D+ D D+D D DE +
Sbjct: 425 EEDEDGEDQEDDDDDDEDDDEED 357
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.312 0.130 0.366
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,603,046
Number of Sequences: 28460
Number of extensions: 66990
Number of successful extensions: 302
Number of sequences better than 10.0: 30
Number of HSP's better than 10.0 without gapping: 297
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 300
length of query: 408
length of database: 4,897,600
effective HSP length: 92
effective length of query: 316
effective length of database: 2,279,280
effective search space: 720252480
effective search space used: 720252480
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 56 (26.2 bits)
Lotus: description of TM0018.4