BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0016.5
(733 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP063694 101 5e-22
AV766088 84 1e-16
BP033602 64 7e-11
AV764934 51 8e-07
TC18927 similar to PIR|AI2934|AI2934 chromate transport protein ... 44 9e-05
BP058111 43 2e-04
BP073434 42 3e-04
BP054557 42 3e-04
AV427609 40 0.001
TC9337 similar to UP|PME_PRUPE (Q43062) Pectinesterase PPE8B pre... 39 0.004
BP081717 39 0.004
BP033820 38 0.007
BP063902 38 0.007
BP066067 37 0.009
BP053302 37 0.015
TC12255 36 0.025
TC9521 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, part... 34 0.095
TC11885 similar to UP|Q8LF59 (Q8LF59) DNA-binding protein, parti... 34 0.095
BP030995 34 0.095
TC10011 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, par... 33 0.12
>BP063694
Length = 511
Score = 101 bits (251), Expect = 5e-22
Identities = 51/125 (40%), Positives = 72/125 (56%)
Frame = -2
Query: 464 NFELWHKRLGHPNSNVLYELLQSGVLGNKETPSLSTIKFDCTYCKLGKSKILPFPNHQSN 523
+FELWH RLGH N +++ +L + G L + S+ CT C++ KSK L F ++
Sbjct: 369 SFELWHSRLGHVNFDIIKQLHKHGCL---DVSSILPKPICCTSCQMAKSKRLVFHDNNKR 199
Query: 524 ISQTFDMIHSDLWGITPVISHANYKYFVTFIDDFSRFTWVYFLRSKDEVFSAFKFFHAYV 583
S D+IH DL G +PV S + YFV F+DDFSRFTW Y L+ K + F ++
Sbjct: 198 ASAVLDLIHCDLRGPSPVASIDGFSYFVIFVDDFSRFTWFYPLKRKSDFSDVLLRFKVFM 19
Query: 584 ETQFS 588
E +FS
Sbjct: 18 ENRFS 4
>AV766088
Length = 501
Score = 83.6 bits (205), Expect = 1e-16
Identities = 48/133 (36%), Positives = 70/133 (52%)
Frame = -3
Query: 441 PLCFSMSPCSSLPFVSCHSATVVNFELWHKRLGHPNSNVLYELLQSGVLGNKETPSLSTI 500
PL SP P + LWH RLGHP+S+ L L+S + E + S +
Sbjct: 385 PLMRCNSPGDLYPVTLSFPFAGIAQSLWHSRLGHPSSSAL-RYLRSNKFISYELLNYSPV 209
Query: 501 KFDCTYCKLGKSKILPFPNHQSNISQTFDMIHSDLWGITPVISHANYKYFVTFIDDFSRF 560
C C GK LPF + + FD++HSDLW +PV+S A ++++V F+DDF+ F
Sbjct: 208 ---CESCVFGKHVRLPFVSSNNVTVMPFDILHSDLW-TSPVLSSAGHRFYVLFLDDFTDF 41
Query: 561 TWVYFLRSKDEVF 573
W + L +K +VF
Sbjct: 40 LWTFPLSNKSQVF 2
>BP033602
Length = 533
Score = 64.3 bits (155), Expect = 7e-11
Identities = 34/90 (37%), Positives = 49/90 (53%)
Frame = +3
Query: 467 LWHKRLGHPNSNVLYELLQSGVLGNKETPSLSTIKFDCTYCKLGKSKILPFPNHQSNISQ 526
LWH RLGHPN L L + N SL +C C L K++ P+ + + S+
Sbjct: 276 LWHNRLGHPNFQYLRHLFPD-LFKNVNCSSL-----ECESCVLAKNQRAPYYSQPYHASR 437
Query: 527 TFDMIHSDLWGITPVISHANYKYFVTFIDD 556
F +IHSD+WG + + + ++FVTFIDD
Sbjct: 438 PFYLIHSDVWGPSKITTQFGKRWFVTFIDD 527
>AV764934
Length = 406
Score = 50.8 bits (120), Expect = 8e-07
Identities = 39/101 (38%), Positives = 51/101 (49%), Gaps = 7/101 (6%)
Frame = -2
Query: 450 SSLPFVSCHSATV---VNFELWHKRLGHPNSNVLYELLQSGVLGNKETPSLSTIKFDCTY 506
SS P H ++V V WH RLGHP+ + L +L S N T++F T
Sbjct: 276 SSSPVSGRHPSSVSAFVTLSTWHSRLGHPHLDNLKRVLSSC---NVSYSPKDTVEFR-TA 109
Query: 507 CKLGKSKILPFPNHQSNISQT----FDMIHSDLWGITPVIS 543
C LGK+ LP S +S T F++I+SDLWG PV S
Sbjct: 108 CCLGKAHRLP-----SQMSTTTYLPFELIYSDLWGPAPVCS 1
>TC18927 similar to PIR|AI2934|AI2934 chromate transport protein chrA
[imported] - Agrobacterium tumefaciens
(strain C58, Dupont) {Agrobacterium tumefaciens;},
partial (6%)
Length = 561
Score = 43.9 bits (102), Expect = 9e-05
Identities = 22/62 (35%), Positives = 29/62 (46%)
Frame = -2
Query: 225 KPRGRDLSAVQCFCCKGFGHYASNCPRKSCNYCKKDGHVIKECPIRPPKKNATTFTTSVH 284
+P D S + C C GH+A+ CP C C+K GH K+C PK A T +
Sbjct: 362 RPTQSDTSEIVCHRCSKKGHFANRCPDLVCWNCQKTGHSGKDC--TNPKVEAATNAIAAR 189
Query: 285 SP 286
P
Sbjct: 188 RP 183
>BP058111
Length = 570
Score = 42.7 bits (99), Expect = 2e-04
Identities = 26/69 (37%), Positives = 34/69 (48%), Gaps = 2/69 (2%)
Frame = -1
Query: 467 LWHKRLGHPNSNVLYELLQSGVLGNKETPSLSTIKFD--CTYCKLGKSKILPFPNHQSNI 524
LWH RLGH +S L L K P ++ K C C + K K L FPN +
Sbjct: 186 LWHLRLGHTSSKKLAIL-------QKNFPFITCKKITSPCDTCHMAKQKKLSFPNSVTLS 28
Query: 525 SQTFDMIHS 533
S+ FD+IH+
Sbjct: 27 SEIFDLIHT 1
>BP073434
Length = 490
Score = 42.4 bits (98), Expect = 3e-04
Identities = 29/73 (39%), Positives = 36/73 (48%), Gaps = 1/73 (1%)
Frame = -1
Query: 445 SMSPCSSLPFVSCHSATVVN-FELWHKRLGHPNSNVLYELLQSGVLGNKETPSLSTIKFD 503
S+ S V+ + T N F +WH RLGHP+ + L +LQS N S F
Sbjct: 244 SVDRSPSFDIVNTITTTTTNKFLIWHSRLGHPHQDALQAVLQS---CNIHLSSRDMTDF- 77
Query: 504 CTYCKLGKSKILP 516
C C LGKS ILP
Sbjct: 76 CYACCLGKSHILP 38
>BP054557
Length = 538
Score = 42.0 bits (97), Expect = 3e-04
Identities = 40/153 (26%), Positives = 62/153 (40%), Gaps = 12/153 (7%)
Frame = -2
Query: 340 DYGASNHMANNAEALTNITQNFGNLKIQVANGN----HLPITAIGDISTSLNDVYVSPGL 395
D GA++H+ +N + + Q L + + NG H+ + L V P
Sbjct: 531 DTGATHHVCHNLDVFQALRQVHPVL-LSMPNGQKEIAHMADSVYFSEKFFLAXVLYVPSF 355
Query: 396 TSNLIFVGQLVDN-DCRVAFSKSGCLVQDQHSGKLIARGPKVGRLFPLCFSMSPCSSLPF 454
NLI V LV + +CR+ CL+Q++ + K I + G L+ + P
Sbjct: 354 NFNLISVSALVKSLNCRLVIFDECCLLQERPTLKTIGAAEERGGLYAVIDPAVKAKHPPA 175
Query: 455 VSCHSATVVNFE-------LWHKRLGHPNSNVL 480
V +F LWH RLGH +S L
Sbjct: 174 EGNIKTQVSHFNFCQFDCNLWHLRLGHTSSKKL 76
>AV427609
Length = 332
Score = 40.4 bits (93), Expect = 0.001
Identities = 21/76 (27%), Positives = 32/76 (41%), Gaps = 1/76 (1%)
Frame = +1
Query: 54 YADWEVKDNQVLAWIIRFVDPNIVLNL-RPFSTAAKMWAYLKKIYNQNNTARRFQLEHDI 112
Y W D VL WI + +++ + P STA W +L ++ N AR LE +
Sbjct: 85 YEQWATLDATVLQWIYSTISVDLMTTIMEPGSTAFTAWTHLADLFQDNQNARAVTLEQEF 264
Query: 113 AIFQQEILSISEFYSQ 128
+ + E Y Q
Sbjct: 265 STVRMEAFPTVAAYCQ 312
>TC9337 similar to UP|PME_PRUPE (Q43062) Pectinesterase PPE8B precursor
(Pectin methylesterase) (PE) , partial (54%)
Length = 1145
Score = 38.5 bits (88), Expect = 0.004
Identities = 18/40 (45%), Positives = 26/40 (65%)
Frame = +3
Query: 504 CTYCKLGKSKILPFPNHQSNISQTFDMIHSDLWGITPVIS 543
C C LGK LPF + ++ + FD++HSDLW +PV+S
Sbjct: 1029 CDSCVLGKHVRLPFSSSETITLRPFDILHSDLW-TSPVLS 1145
>BP081717
Length = 291
Score = 38.5 bits (88), Expect = 0.004
Identities = 20/55 (36%), Positives = 28/55 (50%), Gaps = 1/55 (1%)
Frame = -1
Query: 465 FELWHKRLGHPNSNVLYELLQSGVLGNKETPSLST-IKFDCTYCKLGKSKILPFP 518
F++WH RLGHP+S VL + +K P + C +C + K LPFP
Sbjct: 174 FDMWHFRLGHPSSKVLQHI-------SKVFPYVKLHSNIICDFCHMAKQTKLPFP 31
>BP033820
Length = 537
Score = 37.7 bits (86), Expect = 0.007
Identities = 33/148 (22%), Positives = 55/148 (36%), Gaps = 50/148 (33%)
Frame = -2
Query: 386 LNDVYVSPGLTSNLIFVGQLV-DNDCRVAFSKSGCLVQDQHSGKLIARG----------- 433
LN + + P +T NL+ V + DN+ F + C V+ Q + +L+ G
Sbjct: 494 LNKLLLVPSITKNLVSVSRFAKDNNVYFTFHANFCAVRHQDTSELLLTGSVGDDGLYYFD 315
Query: 434 ----------PKVGRLFPLCFSMSPCSSLPFVS--------------------------- 456
P LF P + S
Sbjct: 314 GPPSQAVSHSPVKASLFQSSLHSQPLAQAGLASQPVGNKASSAIDLPSSLVNSSVSSSAP 135
Query: 457 CHSATVVN-FELWHKRLGHPNSNVLYEL 483
C+S++ ++ +ELWH R+GHP+ +VL +L
Sbjct: 134 CNSSSNISIYELWHNRMGHPHHDVLKQL 51
>BP063902
Length = 494
Score = 37.7 bits (86), Expect = 0.007
Identities = 29/92 (31%), Positives = 42/92 (45%)
Frame = -3
Query: 426 SGKLIARGPKVGRLFPLCFSMSPCSSLPFVSCHSATVVNFELWHKRLGHPNSNVLYELLQ 485
+G + R +G L+P+ S PF S+ +WH RLGHP S+ L L
Sbjct: 270 TGMPLLRCNSLGDLYPVT------RSSPFAGLASS------VWHHRLGHPASSALNHLRN 127
Query: 486 SGVLGNKETPSLSTIKFDCTYCKLGKSKILPF 517
+ ++ + + S S C C LGK LPF
Sbjct: 126 NKLIFCEPSRSSSV----CDSCVLGKHVRLPF 43
Score = 28.5 bits (62), Expect = 4.0
Identities = 10/18 (55%), Positives = 13/18 (71%)
Frame = -2
Query: 331 SPNSSPWYFDYGASNHMA 348
+P +PWY D GAS+H A
Sbjct: 334 TPPDTPWYMDTGASSHTA 281
>BP066067
Length = 505
Score = 37.4 bits (85), Expect = 0.009
Identities = 23/81 (28%), Positives = 39/81 (47%), Gaps = 10/81 (12%)
Frame = -2
Query: 337 WYFDYGASNHMANNAEALTNITQNFGNLKIQVANGNHLPITAIG----------DISTSL 386
W D GAS+H+ N+ + L G ++ V +G LPI +IG + SL
Sbjct: 297 W*ADSGASHHVTNDPQHLMESITMPGQEQVYVCSGQGLPILSIGFTSFSSPLISNTKFSL 118
Query: 387 NDVYVSPGLTSNLIFVGQLVD 407
D + P +T N++ + L++
Sbjct: 117 KDFFHLPTITKNIVVLVGLLE 55
>BP053302
Length = 432
Score = 36.6 bits (83), Expect = 0.015
Identities = 25/70 (35%), Positives = 34/70 (47%), Gaps = 7/70 (10%)
Frame = -1
Query: 421 VQDQHSGKLIARGPKVGRLFPLCF-SMSPCSSLPFVSCHSATVV------NFELWHKRLG 473
+ D +SGK+I R + G L+ L S SP SC + V + LWH RLG
Sbjct: 249 ILDLNSGKMIGRAKESGGLYYLDLGSASPLPPKTISSCFESFFVLNNNDDHVMLWHLRLG 70
Query: 474 HPNSNVLYEL 483
HP+ + L L
Sbjct: 69 HPSFHYLKPL 40
>TC12255
Length = 558
Score = 35.8 bits (81), Expect = 0.025
Identities = 16/40 (40%), Positives = 25/40 (62%)
Frame = +1
Query: 445 SMSPCSSLPFVSCHSATVVNFELWHKRLGHPNSNVLYELL 484
S +PC+S S+ + +ELWH R+GHP+ +VL + L
Sbjct: 409 SSAPCNS-------SSNISIYELWHNRMGHPHHDVLKQTL 507
>TC9521 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, partial (56%)
Length = 598
Score = 33.9 bits (76), Expect = 0.095
Identities = 17/51 (33%), Positives = 24/51 (46%), Gaps = 4/51 (7%)
Frame = +1
Query: 227 RGRDLSAVQCFCCKGFGHYASNCP----RKSCNYCKKDGHVIKECPIRPPK 273
RG + +CF C GH+A +C + C C + GH+ K C P K
Sbjct: 364 RGPPPGSGRCFNCGIDGHWARDCKAGDWKNKCYRCGERGHIEKNCKNSPKK 516
>TC11885 similar to UP|Q8LF59 (Q8LF59) DNA-binding protein, partial (26%)
Length = 555
Score = 33.9 bits (76), Expect = 0.095
Identities = 16/34 (47%), Positives = 18/34 (52%), Gaps = 1/34 (2%)
Frame = +2
Query: 236 CFCCKGFGHYASNCPRKS-CNYCKKDGHVIKECP 268
C C GH AS C KS C CK+ GH+ CP
Sbjct: 401 CHNCGLPGHIASECTTKSLCWNCKEPGHMASSCP 502
Score = 33.9 bits (76), Expect = 0.095
Identities = 14/33 (42%), Positives = 18/33 (54%), Gaps = 1/33 (3%)
Frame = +2
Query: 236 CFCCKGFGHYASNCPRKS-CNYCKKDGHVIKEC 267
C CK GH+A CP + C+ C GH+ EC
Sbjct: 344 CKNCKRPGHFARECPNVAICHNCGLPGHIASEC 442
>BP030995
Length = 458
Score = 33.9 bits (76), Expect = 0.095
Identities = 33/130 (25%), Positives = 52/130 (39%), Gaps = 26/130 (20%)
Frame = -3
Query: 386 LNDVYVSPGLTSNLIFVGQLV-DNDCRVAFSKSGCLVQDQHSGKLIARGPKVGRLFP--- 441
LN++ PG+ NL V N+ F CLV+ Q + + + +G L
Sbjct: 420 LNNLLHVPGVCKNLSSVSXFCKQNNVFFEFHSDSCLVKSQETEETLLQGTLRNGLCAFDN 241
Query: 442 -----------------LCFSMSPCSSLPFVSCHSATV-----VNFELWHKRLGHPNSNV 479
L S SP S+L VS S+ ++ LWH RLGH + +
Sbjct: 240 LLHKVNLHSASTLSNSALLSSSSPASALS-VSVFSSNKCAQADIDSRLWHSRLGHASQKI 64
Query: 480 LYELLQSGVL 489
+ +L+ +L
Sbjct: 63 VSHVLKHVIL 34
>TC10011 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, partial (62%)
Length = 684
Score = 33.5 bits (75), Expect = 0.12
Identities = 19/53 (35%), Positives = 25/53 (46%), Gaps = 4/53 (7%)
Frame = +2
Query: 227 RGRDLSAVQCFCCKGFGHYASNCP----RKSCNYCKKDGHVIKECPIRPPKKN 275
RG + +CF C GH+A +C + C C GHV + C PKKN
Sbjct: 344 RGPPPGSGRCFNCGLDGHWARDCKAGDWKNKCYRCGDRGHVERNCK-NSPKKN 499
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.331 0.141 0.457
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 14,603,595
Number of Sequences: 28460
Number of extensions: 234166
Number of successful extensions: 1631
Number of sequences better than 10.0: 70
Number of HSP's better than 10.0 without gapping: 1584
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1615
length of query: 733
length of database: 4,897,600
effective HSP length: 97
effective length of query: 636
effective length of database: 2,136,980
effective search space: 1359119280
effective search space used: 1359119280
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.9 bits)
S2: 59 (27.3 bits)
Lotus: description of TM0016.5