BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0010.3
(172 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC17885 UP|PSA7_CICAR (Q9SXU1) Proteasome subunit alpha type 7 ... 28 0.85
AV408845 27 1.4
AV774655 27 1.4
TC15301 similar to UP|EX13_ARATH (Q9M9P0) Alpha-expansin 13 prec... 27 1.9
TC13758 similar to GB|AAL87371.1|19548015|AY081718 AT4g33920/F17... 27 2.5
BP056955 27 2.5
TC8035 UP|GLN1_LOTJA (Q42899) Glutamine synthetase, cytosolic is... 26 3.2
TC16802 similar to GB|AAP31963.1|30387595|BT006619 At1g01300 {Ar... 26 3.2
TC15907 weakly similar to UP|7MT6_MEDSA (O22308) Isoflavone-7-O-... 26 3.2
AV781158 26 4.2
TC16413 similar to GB|AAF63224.1|7532410|AC002329 3-hydroxy-3-me... 26 4.2
BP068044 26 4.2
BU494330 25 5.5
TC14089 similar to UP|RLA0_SOYBN (P50346) 60S acidic ribosomal p... 25 5.5
TC14087 homologue to UP|Q9ZP90 (Q9ZP90) Ferritin, partial (78%) 25 5.5
TC16554 homologue to UP|AAN71719 (AAN71719) Glycogen synthase ki... 25 5.5
TC7895 homologue to UP|Q8GTE3 (Q8GTE3) Ribosomal protein S3a, co... 25 5.5
AV423882 25 7.2
BP045724 25 7.2
BP038556 25 9.4
>TC17885 UP|PSA7_CICAR (Q9SXU1) Proteasome subunit alpha type 7 (20S
proteasome alpha subunit D) (20S proteasome subunit
alpha-4) , partial (49%)
Length = 444
Score = 28.1 bits (61), Expect = 0.85
Identities = 20/55 (36%), Positives = 28/55 (50%), Gaps = 8/55 (14%)
Frame = +2
Query: 18 LVRVREEEEEE--------EEAYFVSMAKANLSSLPERIAASSSTLTPNNPSGEA 64
L+ REEEE E EA ++ M + + SLP I++ SS TP+ P A
Sbjct: 11 LLNFREEEESEVD*A*RFESEAEWLGMTERSRCSLPMAISSRSS--TPSKPFARA 169
>AV408845
Length = 429
Score = 27.3 bits (59), Expect = 1.4
Identities = 12/21 (57%), Positives = 16/21 (76%)
Frame = -3
Query: 23 EEEEEEEEAYFVSMAKANLSS 43
EEEEEEEE FV + K+ ++S
Sbjct: 118 EEEEEEEEGEFVGLEKSPVNS 56
>AV774655
Length = 485
Score = 27.3 bits (59), Expect = 1.4
Identities = 17/40 (42%), Positives = 23/40 (57%), Gaps = 4/40 (10%)
Frame = -1
Query: 20 RVREEEEEEEEAYFVSMAKAN----LSSLPERIAASSSTL 55
R EEEEEEEE + VS +N LS+ P +S+ +L
Sbjct: 173 REEEEEEEEEEEFRVSTMDSNSVIALSTSPASTRSSTRSL 54
>TC15301 similar to UP|EX13_ARATH (Q9M9P0) Alpha-expansin 13 precursor
(At-EXP13) (AtEx13) (Ath-ExpAlpha-1.22), partial (45%)
Length = 507
Score = 26.9 bits (58), Expect = 1.9
Identities = 22/61 (36%), Positives = 28/61 (45%), Gaps = 2/61 (3%)
Frame = -3
Query: 83 PSSSRNNDREVIAVVKLISHTARNFP--GVFFLGKPSAVLPVLADILPSYADPLFRSRHR 140
P + N R A V+ SH+A +P F G+ S L +ADPL RSR R
Sbjct: 370 PHTGAQNTRRRSAPVQTASHSAPPWPCRSQFLRGRRSRTRR-----LQRHADPLQRSRWR 206
Query: 141 V 141
V
Sbjct: 205 V 203
>TC13758 similar to GB|AAL87371.1|19548015|AY081718 AT4g33920/F17I5_110
{Arabidopsis thaliana;}, partial (28%)
Length = 466
Score = 26.6 bits (57), Expect = 2.5
Identities = 17/54 (31%), Positives = 27/54 (49%), Gaps = 5/54 (9%)
Frame = +1
Query: 10 TFTFPHSTLVRVREEEEEEEEAY-----FVSMAKANLSSLPERIAASSSTLTPN 58
T T ++ +EEEEEEEE++ F+ A+L P R+ + T P+
Sbjct: 13 TVTHTYALTQPTQEEEEEEEESFHIETSFIDSDHASLVLPPSRLYLPTVT*RPS 174
>BP056955
Length = 581
Score = 26.6 bits (57), Expect = 2.5
Identities = 12/14 (85%), Positives = 12/14 (85%)
Frame = +1
Query: 17 TLVRVREEEEEEEE 30
TL R REEEEEEEE
Sbjct: 25 TLSRAREEEEEEEE 66
>TC8035 UP|GLN1_LOTJA (Q42899) Glutamine synthetase, cytosolic isozyme
(Glutamate--ammonia ligase) (GS1) , complete
Length = 1468
Score = 26.2 bits (56), Expect = 3.2
Identities = 19/46 (41%), Positives = 24/46 (51%), Gaps = 7/46 (15%)
Frame = +1
Query: 12 TFPHSTLVRVRE-EEEEEEEAYFVSMA------KANLSSLPERIAA 50
T P R RE EEEEEEE+ F+ M+ NLS ++I A
Sbjct: 4 TRPDEDSKRKREKEEEEEEESSFLKMSLLSDLINLNLSETTDKIIA 141
>TC16802 similar to GB|AAP31963.1|30387595|BT006619 At1g01300 {Arabidopsis
thaliana;}, partial (28%)
Length = 812
Score = 26.2 bits (56), Expect = 3.2
Identities = 19/52 (36%), Positives = 25/52 (47%), Gaps = 6/52 (11%)
Frame = +3
Query: 12 TFPHSTLVRVREEEEEEEEAYFVSMAKA------NLSSLPERIAASSSTLTP 57
+ PH + E EEEEEE +S+ ++ LS I A SST TP
Sbjct: 138 SLPHQPTLSWPESEEEEEEEEELSIPESEGSDPETLSLQLHHIDALSSTKTP 293
>TC15907 weakly similar to UP|7MT6_MEDSA (O22308)
Isoflavone-7-O-methytransferase 6
(Isoflavone-O-methytransferase 6) (7-IOMT-6) , partial
(23%)
Length = 612
Score = 26.2 bits (56), Expect = 3.2
Identities = 17/52 (32%), Positives = 24/52 (45%)
Frame = +2
Query: 6 KELITFTFPHSTLVRVREEEEEEEEAYFVSMAKANLSSLPERIAASSSTLTP 57
K L+ F H+ + + EEEE+ EAY ++ A L S S L P
Sbjct: 287 KRLMRF-LGHNGIFAIAGEEEEDHEAYDLTPASKLLISKAAHAGRSDPCLAP 439
>AV781158
Length = 511
Score = 25.8 bits (55), Expect = 4.2
Identities = 19/42 (45%), Positives = 22/42 (52%), Gaps = 5/42 (11%)
Frame = +1
Query: 23 EEEEEEEEAYFV----SMAKANLSSLPERIAA-SSSTLTPNN 59
EEEEEEEE F + L SL AA S+S + PNN
Sbjct: 16 EEEEEEEEDLFTLLH*KFPRWGLESLHTSSAAGSTSRVDPNN 141
>TC16413 similar to GB|AAF63224.1|7532410|AC002329
3-hydroxy-3-methylglutaryl-coenzyme A reductase 2
{Arabidopsis thaliana;}, partial (41%)
Length = 674
Score = 25.8 bits (55), Expect = 4.2
Identities = 33/118 (27%), Positives = 50/118 (41%), Gaps = 4/118 (3%)
Frame = +2
Query: 20 RVREEEEEEEEAYFVSMAKANLSSL--PERIAASSSTLTPNNPSGEALEVRFCTVLPNLL 77
RVR ++ +EEEA +MA S L P SSS P+ + +R C + N
Sbjct: 5 RVRTDQHQEEEAALHTMAATTSSWLLCPLNFKGSSSNRMGIPPT--TIRIR-CNLSAN-- 169
Query: 78 HAYVVPSSSRNNDREVIAVVKLIS-HTARNF-PGVFFLGKPSAVLPVLADILPSYADP 133
H +++ + + + VK + T N PG FLG + L D + DP
Sbjct: 170 HKSTTLATTESEPKPIYTSVKTFAPATVANLGPGFDFLG---CAVDGLGDTVSLRVDP 334
>BP068044
Length = 266
Score = 25.8 bits (55), Expect = 4.2
Identities = 14/31 (45%), Positives = 16/31 (51%)
Frame = -2
Query: 141 VFFEALGSLLSLFRSGARDASTHFFLDPMLL 171
VFF LLSLF S S+ FF+ P L
Sbjct: 148 VFFCVXDLLLSLFSSSPAQTSSLFFIGPWAL 56
>BU494330
Length = 208
Score = 25.4 bits (54), Expect = 5.5
Identities = 13/38 (34%), Positives = 20/38 (52%)
Frame = +2
Query: 49 AASSSTLTPNNPSGEALEVRFCTVLPNLLHAYVVPSSS 86
+A + TLTP E+RF +PNLL + ++S
Sbjct: 5 SARAQTLTPTKKLISLREIRFPNPIPNLLACSEIGAAS 118
>TC14089 similar to UP|RLA0_SOYBN (P50346) 60S acidic ribosomal protein P0,
partial (80%)
Length = 907
Score = 25.4 bits (54), Expect = 5.5
Identities = 23/89 (25%), Positives = 37/89 (40%), Gaps = 6/89 (6%)
Frame = -1
Query: 15 HSTLVRVREEEEEEEEAYF------VSMAKANLSSLPERIAASSSTLTPNNPSGEALEVR 68
HSTLV + E EE V+ +++ + + SS L +N L+V
Sbjct: 592 HSTLVNLGRNVEHLEERSLRGVEPCVARRNNHINWSNQTNTSRSSNLVLSNLLANLLQVT 413
Query: 69 FCTVLPNLLHAYVVPSSSRNNDREVIAVV 97
FC PN+ S++ D+ I +V
Sbjct: 412 FCEDQPNI--------SNKEGDKVEIDIV 350
>TC14087 homologue to UP|Q9ZP90 (Q9ZP90) Ferritin, partial (78%)
Length = 1120
Score = 25.4 bits (54), Expect = 5.5
Identities = 15/52 (28%), Positives = 25/52 (47%), Gaps = 4/52 (7%)
Frame = -3
Query: 21 VREEEEEEEEAYF----VSMAKANLSSLPERIAASSSTLTPNNPSGEALEVR 68
+ EEEEEEE ++F + + N +L E A + + + G +E R
Sbjct: 188 IEEEEEEEETSFFETSPTTGERENTETLEEERAIRAKSQNGYSEKGREMEKR 33
>TC16554 homologue to UP|AAN71719 (AAN71719) Glycogen synthase kinase 3 beta
protein kinase DWARF12 , partial (71%)
Length = 927
Score = 25.4 bits (54), Expect = 5.5
Identities = 12/22 (54%), Positives = 13/22 (58%)
Frame = -3
Query: 10 TFTFPHSTLVRVREEEEEEEEA 31
T +P R RE EEEEEEA
Sbjct: 142 TSPYPQPWRTREREREEEEEEA 77
>TC7895 homologue to UP|Q8GTE3 (Q8GTE3) Ribosomal protein S3a, complete
Length = 1106
Score = 25.4 bits (54), Expect = 5.5
Identities = 18/41 (43%), Positives = 19/41 (45%), Gaps = 1/41 (2%)
Frame = +1
Query: 19 VRVREEEEEEEEAYFVSMAKANLSSLP-ERIAASSSTLTPN 58
VR EEEEEEEE A P ER AA L P+
Sbjct: 13 VRPSEEEEEEEEEKKQPWLSARTRGFPRERRAARRRPLIPS 135
>AV423882
Length = 178
Score = 25.0 bits (53), Expect = 7.2
Identities = 14/29 (48%), Positives = 21/29 (72%), Gaps = 1/29 (3%)
Frame = -1
Query: 37 AKANLSSL-PERIAASSSTLTPNNPSGEA 64
A+A+ S++ P R AASSST + NP+ E+
Sbjct: 160 AEASSSTISPTRSAASSSTTSAVNPTAES 74
>BP045724
Length = 490
Score = 25.0 bits (53), Expect = 7.2
Identities = 20/72 (27%), Positives = 35/72 (47%), Gaps = 5/72 (6%)
Frame = -2
Query: 12 TFPHSTLVRVREEEEEEEEAYFVSMAKANLSSLPERIAASSSTLTPN-----NPSGEALE 66
+FP + V+ + +EEEEEEA P I++SS L P+ + S +
Sbjct: 384 SFPSPSSVKRKCKEEEEEEA-------------PPSISSSSDHLIPSSELTFHDSPLTFD 244
Query: 67 VRFCTVLPNLLH 78
+ V+P++L+
Sbjct: 243 SDYMGVIPDVLY 208
>BP038556
Length = 555
Score = 24.6 bits (52), Expect = 9.4
Identities = 10/17 (58%), Positives = 13/17 (75%)
Frame = -1
Query: 65 LEVRFCTVLPNLLHAYV 81
+EVRFC+V NL+H V
Sbjct: 60 IEVRFCSVHLNLIHCSV 10
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.321 0.135 0.380
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,928,324
Number of Sequences: 28460
Number of extensions: 41018
Number of successful extensions: 408
Number of sequences better than 10.0: 42
Number of HSP's better than 10.0 without gapping: 328
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 383
length of query: 172
length of database: 4,897,600
effective HSP length: 84
effective length of query: 88
effective length of database: 2,506,960
effective search space: 220612480
effective search space used: 220612480
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 52 (24.6 bits)
Lotus: description of TM0010.3