BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0008.9
(276 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP041062 90 4e-19
BP036389 72 1e-13
TC17229 similar to UP|O49595 (O49595) HMG protein (AT3G51880/ORF... 32 0.090
TC10635 similar to UP|Q946U8 (Q946U8) Long cell-linked locus pro... 30 0.34
TC14520 similar to UP|Q8W194 (Q8W194) Zinc finger protein LSD2, ... 30 0.45
BP055784 30 0.45
BE122583 30 0.45
AV428537 30 0.58
TC8872 homologue to UP|Q8L5G5 (Q8L5G5) Alpha-expansin 3, partial... 28 1.7
AV409973 28 2.2
TC15958 similar to GB|AAP37838.1|30725632|BT008479 At4g24220 {Ar... 28 2.2
TC16651 similar to PIR|S67496|S67496 glutamate synthase (ferredo... 28 2.2
AV772700 28 2.2
TC13708 28 2.2
TC18102 similar to UP|PFD2_ARATH (Q9LJ98) Probable prefoldin sub... 27 2.9
TC8259 similar to UP|HMGL_SOYBN (P26585) HMG1/2-like protein (SB... 27 2.9
TC14233 homologue to UP|Q41027 (Q41027) PsHSC71.0, partial (67%) 27 3.8
TC13632 similar to UP|TL17_ARATH (P81760) Thylakoid lumenal 17.4... 27 3.8
TC8468 similar to UP|BRU1_SOYBN (P35694) Brassinosteroid-regulat... 27 3.8
TC14083 similar to UP|CRTC_RICCO (P93508) Calreticulin precursor... 27 3.8
>BP041062
Length = 565
Score = 90.1 bits (222), Expect = 4e-19
Identities = 40/56 (71%), Positives = 48/56 (85%)
Frame = -1
Query: 166 IPMSNFVNKPPEKRQRTPSAYNRFIKEEIKRLKAENPDMAHKEAFSTAAKNWANFP 221
IP N+PPEKRQR PSAYNRFIK+EI+R+KA NPD++H+EAFS AAKNWA+FP
Sbjct: 466 IPKPPPANRPPEKRQRVPSAYNRFIKDEIQRIKAGNPDISHREAFSAAAKNWAHFP 299
>BP036389
Length = 439
Score = 72.0 bits (175), Expect = 1e-13
Identities = 38/69 (55%), Positives = 45/69 (65%), Gaps = 4/69 (5%)
Frame = +3
Query: 48 TMSTLNHLFDLPEQICYVQCGFCTTILMVSVPCSSLSMVVTVRCGHCTSLLSVNMMKASF 107
T +L+H EQ+CYV C C TIL VSVP +SL VTVRCGHCT+LL VN M+A
Sbjct: 174 TTLSLDHQLPPSEQLCYVHCNICDTILAVSVPSTSLFKTVTVRCGHCTNLLPVN-MRALL 350
Query: 108 VP----FHL 112
+P FHL
Sbjct: 351 LPSPNQFHL 377
>TC17229 similar to UP|O49595 (O49595) HMG protein (AT3G51880/ORF13),
partial (66%)
Length = 540
Score = 32.3 bits (72), Expect = 0.090
Identities = 24/88 (27%), Positives = 39/88 (44%), Gaps = 6/88 (6%)
Frame = +3
Query: 174 KPPEKRQRTPSAYNRFIKEEIKRLKAENPDMAHKEAFSTA-AKNW-----ANFPPTQCKG 227
K P K +R PSA+ F+++ K KAENP++ A A + W A P + K
Sbjct: 249 KDPNKPKRPPSAFFVFLEDFRKTFKAENPNVKGVSAVGKAGGEKWKSLTKAEKAPYEAKA 428
Query: 228 DEEGVLHPCITSMCGTFRSMKKDKVSEE 255
+ + + + ++ D V EE
Sbjct: 429 AKRKAEYEKLINAYNNKQASTADDVDEE 512
>TC10635 similar to UP|Q946U8 (Q946U8) Long cell-linked locus protein,
partial (16%)
Length = 1243
Score = 30.4 bits (67), Expect = 0.34
Identities = 13/48 (27%), Positives = 27/48 (56%)
Frame = +3
Query: 8 SSTINASFSLCQSKVLHHLSITSFSSSFTHTYSHPHIQKDTMSTLNHL 55
SS + +FS S ++H ++++ ++ + + SHPH +T T H+
Sbjct: 120 SSMMPTAFSTAVSFIIHMVNLSMVKTTISLSLSHPHYASNTPETHIHI 263
>TC14520 similar to UP|Q8W194 (Q8W194) Zinc finger protein LSD2, partial
(84%)
Length = 1112
Score = 30.0 bits (66), Expect = 0.45
Identities = 18/52 (34%), Positives = 23/52 (43%), Gaps = 1/52 (1%)
Frame = +2
Query: 61 QICYVQCGFCTTILMVSVPCSSLSMVVTVRCGHCTSLLSVNMMKASF-VPFH 111
Q+ +V CG C T LM S V+C C + +VNM VP H
Sbjct: 509 QVAHVPCGNCRTTLMYPYGAPS------VKCALCHYITNVNMSNGRLQVPMH 646
>BP055784
Length = 361
Score = 30.0 bits (66), Expect = 0.45
Identities = 20/44 (45%), Positives = 25/44 (56%), Gaps = 6/44 (13%)
Frame = +2
Query: 6 CPSSTINASFSLCQSKVLHHLSITSFSSSFTHT------YSHPH 43
C +TI A SLC + + HL +FSSSF+HT YSH H
Sbjct: 152 CARTTIQA-LSLCSAILRTHLPC-NFSSSFSHTHAVVLHYSHNH 277
>BE122583
Length = 361
Score = 30.0 bits (66), Expect = 0.45
Identities = 13/35 (37%), Positives = 21/35 (59%)
Frame = -2
Query: 93 HCTSLLSVNMMKASFVPFHLLASLTHLQVIDYLTL 127
H LL ++ + F+P+H L +L HL++ YL L
Sbjct: 162 HSLQLLELSYV*YFFLPYHYLQTLQHLKLYSYLLL 58
>AV428537
Length = 414
Score = 29.6 bits (65), Expect = 0.58
Identities = 14/29 (48%), Positives = 15/29 (51%)
Frame = +3
Query: 15 FSLCQSKVLHHLSITSFSSSFTHTYSHPH 43
F L QSK HH SFSS + HPH
Sbjct: 42 FFLLQSKTNHHRPFFSFSSLWPQKEQHPH 128
>TC8872 homologue to UP|Q8L5G5 (Q8L5G5) Alpha-expansin 3, partial (82%)
Length = 729
Score = 28.1 bits (61), Expect = 1.7
Identities = 17/44 (38%), Positives = 22/44 (49%), Gaps = 1/44 (2%)
Frame = +1
Query: 63 CYVQCGFCTTILMVSVPCSSLSMVVT-VRCGHCTSLLSVNMMKA 105
CYVQ F +L+ S C +V + + CGHC LLS A
Sbjct: 274 CYVQQWFELWLLL*S*VCE*PQVVPSWLHCGHCHQLLSTKQCSA 405
>AV409973
Length = 415
Score = 27.7 bits (60), Expect = 2.2
Identities = 13/32 (40%), Positives = 19/32 (58%), Gaps = 1/32 (3%)
Frame = -1
Query: 18 CQSKVLHHLSITSFSSSFTHTY-SHPHIQKDT 48
C + + HH ++FSS+ TH Y H H Q+ T
Sbjct: 169 CSNIIRHHGQ*STFSSTRTHHYFDHTHSQQST 74
>TC15958 similar to GB|AAP37838.1|30725632|BT008479 At4g24220 {Arabidopsis
thaliana;}, partial (52%)
Length = 800
Score = 27.7 bits (60), Expect = 2.2
Identities = 16/42 (38%), Positives = 22/42 (52%)
Frame = +2
Query: 97 LLSVNMMKASFVPFHLLASLTHLQVIDYLTLTNSMFIYFSCS 138
LL ++ V F SLTHL ++ L L N +F +FS S
Sbjct: 32 LLPLSKQLEDAVSFKSTHSLTHLLLLLLLLLCNCIFFFFSFS 157
>TC16651 similar to PIR|S67496|S67496 glutamate synthase (ferredoxin) -
spinach (fragment) {Spinacia oleracea;}
, partial (23%)
Length = 1025
Score = 27.7 bits (60), Expect = 2.2
Identities = 14/38 (36%), Positives = 21/38 (54%)
Frame = +1
Query: 164 DLIPMSNFVNKPPEKRQRTPSAYNRFIKEEIKRLKAEN 201
DL+ + N +KR + Y +IKE ++ LKAEN
Sbjct: 439 DLLGGQVYENTEVKKRVALSNPYGDWIKENLRSLKAEN 552
>AV772700
Length = 508
Score = 27.7 bits (60), Expect = 2.2
Identities = 18/68 (26%), Positives = 30/68 (43%), Gaps = 8/68 (11%)
Frame = +2
Query: 128 TNSMFIYFSCSKEDANMTF---NSHSGASMMTYSDCEEEDLIPMSNF-----VNKPPEKR 179
TN+ F + S ++ ++F NS SG + E +SN ++ PP+ +
Sbjct: 272 TNTSFRHLQASTMESGLSFQPFNSESGVRDTIHQSAECSLYYHVSNLYYYFIIDSPPQNK 451
Query: 180 QRTPSAYN 187
RT YN
Sbjct: 452 TRTTHLYN 475
>TC13708
Length = 539
Score = 27.7 bits (60), Expect = 2.2
Identities = 26/107 (24%), Positives = 46/107 (42%), Gaps = 3/107 (2%)
Frame = +3
Query: 125 LTLTNSMFIYFSCSKEDANMTFNSHSGASMM---TYSDCEEEDLIPMSNFVNKPPEKRQR 181
L+ +N F ED +M + + ++ T +E L+ +++ PPE
Sbjct: 231 LSTSNRCFQIKIAQDEDGDMPISYPAQLGLIFAHTERYPDEPPLLNVTSLQGIPPEDL-- 404
Query: 182 TPSAYNRFIKEEIKRLKAENPDMAHKEAFSTAAKNWANFPPTQCKGD 228
R +KE++++ +EN MA T+AK W + Q GD
Sbjct: 405 ------RAVKEKLQQEASENLGMAMIYTLVTSAKEWLDERFCQDSGD 527
>TC18102 similar to UP|PFD2_ARATH (Q9LJ98) Probable prefoldin subunit 2,
partial (83%)
Length = 760
Score = 27.3 bits (59), Expect = 2.9
Identities = 14/39 (35%), Positives = 20/39 (50%)
Frame = -2
Query: 110 FHLLASLTHLQVIDYLTLTNSMFIYFSCSKEDANMTFNS 148
FHL SL + I L + N +F+ F C + +N F S
Sbjct: 453 FHLSISLPYPYFIFSLKVRNFLFLLFQCLIKSSNNLFKS 337
>TC8259 similar to UP|HMGL_SOYBN (P26585) HMG1/2-like protein (SB11
protein), partial (92%)
Length = 942
Score = 27.3 bits (59), Expect = 2.9
Identities = 15/41 (36%), Positives = 21/41 (50%)
Frame = +2
Query: 174 KPPEKRQRTPSAYNRFIKEEIKRLKAENPDMAHKEAFSTAA 214
K P K +R PSA+ F+++ K ENP+ A AA
Sbjct: 290 KDPNKPKRPPSAFFVFMEDFRKIFNKENPENKAVSAVGKAA 412
>TC14233 homologue to UP|Q41027 (Q41027) PsHSC71.0, partial (67%)
Length = 1570
Score = 26.9 bits (58), Expect = 3.8
Identities = 18/62 (29%), Positives = 25/62 (40%), Gaps = 6/62 (9%)
Frame = +2
Query: 168 MSNFVNKPPEKRQRTPSAYNRF------IKEEIKRLKAENPDMAHKEAFSTAAKNWANFP 221
MS +PP KR R+PS R ++ KRL++ N M + S W
Sbjct: 842 MSLLKTRPPAKRTRSPSPMTRVDYPRTTLRRWFKRLRSTNLRMRNTRRRSRPRTPWRTMH 1021
Query: 222 PT 223
T
Sbjct: 1022TT 1027
>TC13632 similar to UP|TL17_ARATH (P81760) Thylakoid lumenal 17.4 kDa
protein, chloroplast precursor (P17.4), partial (22%)
Length = 423
Score = 26.9 bits (58), Expect = 3.8
Identities = 17/70 (24%), Positives = 29/70 (41%), Gaps = 5/70 (7%)
Frame = -2
Query: 99 SVNMMKASFVPFHLLASLTHLQVIDYLTLTNSMFIYF-----SCSKEDANMTFNSHSGAS 153
+ NM K +P H + L +++ + + IYF S S NM + HS +
Sbjct: 422 TTNMYKYFIIPMHSICKLKANRIVQHYKRKANNSIYFQTQHTSTSYPHQNMCMSWHSNLT 243
Query: 154 MMTYSDCEEE 163
+ EE+
Sbjct: 242 KTNIEENEED 213
>TC8468 similar to UP|BRU1_SOYBN (P35694) Brassinosteroid-regulated protein
BRU1 precursor , partial (93%)
Length = 1023
Score = 26.9 bits (58), Expect = 3.8
Identities = 16/38 (42%), Positives = 21/38 (55%), Gaps = 1/38 (2%)
Frame = -3
Query: 18 CQSKVLHHLSITSFSSSFTH-TYSHPHIQKDTMSTLNH 54
C KVL +S TS S H ++ H HI+ TMS +H
Sbjct: 121 C*IKVLVEISSTSCYHSANHYSHQHKHIKAITMSRRSH 8
>TC14083 similar to UP|CRTC_RICCO (P93508) Calreticulin precursor, partial
(92%)
Length = 1693
Score = 26.9 bits (58), Expect = 3.8
Identities = 10/22 (45%), Positives = 14/22 (63%)
Frame = -3
Query: 31 FSSSFTHTYSHPHIQKDTMSTL 52
F S FTH +SHP ++ + TL
Sbjct: 239 FHSDFTHRFSHPSSKRSSKKTL 174
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.319 0.129 0.393
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,030,538
Number of Sequences: 28460
Number of extensions: 96246
Number of successful extensions: 646
Number of sequences better than 10.0: 55
Number of HSP's better than 10.0 without gapping: 631
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 643
length of query: 276
length of database: 4,897,600
effective HSP length: 89
effective length of query: 187
effective length of database: 2,364,660
effective search space: 442191420
effective search space used: 442191420
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 54 (25.4 bits)
Lotus: description of TM0008.9