BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0008.8
(338 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC8966 similar to UP|Q9M5Q3 (Q9M5Q3) S-ribonuclease binding prot... 135 7e-33
TC17807 weakly similar to UP|Q9FHE4 (Q9FHE4) Genomic DNA, chromo... 78 2e-15
TC18699 weakly similar to UP|Q9FHE4 (Q9FHE4) Genomic DNA, chromo... 75 2e-14
TC11037 weakly similar to GB|AAP12885.1|30017303|BT006236 At3g12... 74 3e-14
TC13326 71 2e-13
BP077155 70 4e-13
TC13350 weakly similar to UP|Q9LPJ0 (Q9LPJ0) F6N18.12, partial (... 59 9e-10
BP059128 57 4e-09
TC16011 similar to UP|Q8LAY3 (Q8LAY3) Inhibitor of apoptosis-lik... 54 3e-08
TC15750 similar to UP|Q8LAY3 (Q8LAY3) Inhibitor of apoptosis-lik... 54 4e-08
BP030839 51 3e-07
BP051594 47 6e-06
TC11487 weakly similar to UP|Q8LAY3 (Q8LAY3) Inhibitor of apopto... 45 1e-05
AV779203 45 2e-05
TC17849 44 4e-05
TC8961 similar to GB|BAB71852.1|16902294|AB062739 kinesin-relate... 43 7e-05
BP071727 40 7e-04
AV766525 37 0.004
TC14881 37 0.006
BP043656 35 0.014
>TC8966 similar to UP|Q9M5Q3 (Q9M5Q3) S-ribonuclease binding protein SBP1,
partial (48%)
Length = 611
Score = 135 bits (341), Expect = 7e-33
Identities = 68/159 (42%), Positives = 105/159 (65%), Gaps = 4/159 (2%)
Frame = +2
Query: 183 IVKKLREKEEEIENMNRKNRELAERIKQVAVEAQNWHYRAKYNESVVNALRNNLQQAISH 242
+++ LR++E E+EN+N++N EL +R++Q+AVEA W RA+YNE+++ AL+ NLQQA
Sbjct: 2 VLQILRDQEAEVENINKRNMELEDRMEQLAVEAGAWQQRARYNENMIAALKYNLQQAYVQ 181
Query: 243 GVEQGKEGFGDSEVDDAASYIDPN----NFLSIPGAPMKSIHPRYQGMENLSCRACKAKD 298
KEG GDSEVDD AS + + LS + MK E ++C+AC+ +
Sbjct: 182 S-RDSKEGCGDSEVDDTASCCNGRTLDFHLLSKGNSDMK---------EMMTCKACRVNE 331
Query: 299 VSMLLIPCRHLSLCKDCDGFINVCPVCQMIKTASVEVYL 337
V+M+L+PC+HL LCKDC+ ++ CP+CQ K +EV++
Sbjct: 332 VTMVLLPCKHLCLCKDCESKLSFCPLCQSSKFIGMEVFM 448
>TC17807 weakly similar to UP|Q9FHE4 (Q9FHE4) Genomic DNA, chromosome 5, TAC
clone:K17O22 (AT5g45100/K17O22_9), partial (26%)
Length = 844
Score = 77.8 bits (190), Expect = 2e-15
Identities = 46/158 (29%), Positives = 80/158 (50%), Gaps = 5/158 (3%)
Frame = -3
Query: 90 WSASIPNPNALSTGLRLSYD-DDERNSSVTSASGSMTAPPSIILS----LGDNIRTELDR 144
+ +++ +PN +GL + +R+ TS S A LS D + +
Sbjct: 551 YQSNVCDPNTADSGLTYNLPLQRKRSREFTSELASFPAQQKNKLSSESSFFDQVLYQFQN 372
Query: 145 QQEELDHYIKLQKEQLSRGVRDMKQKHMASLLTSIEKGIVKKLREKEEEIENMNRKNREL 204
QQ E+D + E++ + + K + L I++ + KKL+EK+EE++ M + N L
Sbjct: 371 QQSEIDRVLAHHTEKVRMEMEEHKMRQSRMLACVIQETVAKKLKEKDEEVQKMGKLNWVL 192
Query: 205 AERIKQVAVEAQNWHYRAKYNESVVNALRNNLQQAISH 242
ER+K ++ E Q W A+ NE+ N RNNL+Q ++H
Sbjct: 191 QERVKSLSAENQFWRDLAQTNETTANYHRNNLEQVMAH 78
>TC18699 weakly similar to UP|Q9FHE4 (Q9FHE4) Genomic DNA, chromosome 5, TAC
clone:K17O22 (AT5g45100/K17O22_9), partial (28%)
Length = 537
Score = 74.7 bits (182), Expect = 2e-14
Identities = 51/153 (33%), Positives = 78/153 (50%), Gaps = 1/153 (0%)
Frame = +3
Query: 187 LREKEEEIENMNRKNRELAERIKQVAVEAQNWHYRAKYNESVVNALRNNLQQAISHGVEQ 246
++EK++EI+ + + N L ER+ + E Q W A+ +E+ V +LR++L+Q ++H V
Sbjct: 3 MKEKDDEIQKLAKVNFVLHERVNSLCEENQIWRELAQAHEAAVISLRSDLEQVLAH-VSD 179
Query: 247 GKEGFGDSEVDDAASYIDPNNFLSIPGAPMKSIHPRYQGMENLSCRACKAKDVSMLLIPC 306
G E DDA S N+ + + + NL CR C A++ +LL+PC
Sbjct: 180 SHRGAAAEEEDDAESSCGSNHH-------REEEEEENEAVGNL-CRECGAQESGVLLLPC 335
Query: 307 RHLSLCKDC-DGFINVCPVCQMIKTASVEVYLS 338
RHL L D I CP+C ASV V S
Sbjct: 336 RHLCL*YDVWVQPIRNCPLCHSAINASVHVNFS 434
>TC11037 weakly similar to GB|AAP12885.1|30017303|BT006236 At3g12920
{Arabidopsis thaliana;}, partial (27%)
Length = 730
Score = 74.3 bits (181), Expect = 3e-14
Identities = 36/107 (33%), Positives = 65/107 (60%)
Frame = +1
Query: 134 LGDNIRTELDRQQEELDHYIKLQKEQLSRGVRDMKQKHMASLLTSIEKGIVKKLREKEEE 193
LG++I + Q ++D+ I E++ + + ++ + L+ +IE G++K+L+ KE+E
Sbjct: 406 LGEDISLHIHHHQLDIDNLISQHMEKVRMELEEKRKGQVRRLMEAIEIGMMKRLKTKEDE 585
Query: 194 IENMNRKNRELAERIKQVAVEAQNWHYRAKYNESVVNALRNNLQQAI 240
IE + + N L ER+K + +E Q W A+ NE+ NALR NL+ A+
Sbjct: 586 IERIGKLNLALEERVKSLYIENQIWRELAQTNEATANALRANLEHAL 726
>TC13326
Length = 578
Score = 71.2 bits (173), Expect = 2e-13
Identities = 43/143 (30%), Positives = 65/143 (45%), Gaps = 4/143 (2%)
Frame = +2
Query: 199 RKNRELAERIKQVAVEAQNWHYRAKYNESVVNALRNNLQQAISHGVEQGKEGFGDSEVDD 258
+K EL E + ++ E Q W A+ NE+VV +L N L+Q KE +D
Sbjct: 8 KKKTELEEFLIRLEAENQLWRRVAQENEAVVLSLHNTLEQV--------KERNRGLVAED 163
Query: 259 AASYIDPNN----FLSIPGAPMKSIHPRYQGMENLSCRACKAKDVSMLLIPCRHLSLCKD 314
A SY N + + I + C+ C ++ + +PCRHL CK
Sbjct: 164 AESYCCDENRGNRVMQEGTGETEEIGKETTEQITMVCKCCHSRSSCFMFLPCRHLCSCKA 343
Query: 315 CDGFINVCPVCQMIKTASVEVYL 337
C+ F+ CPVC+M K S+E +
Sbjct: 344 CEPFLQACPVCKMPKKTSIETLI 412
>BP077155
Length = 432
Score = 70.5 bits (171), Expect = 4e-13
Identities = 38/118 (32%), Positives = 60/118 (50%)
Frame = -2
Query: 221 RAKYNESVVNALRNNLQQAISHGVEQGKEGFGDSEVDDAASYIDPNNFLSIPGAPMKSIH 280
+AK E+ +L+ LQ+AI G G G D ++Y+DP + + P +
Sbjct: 425 KAKEQEATAASLQAQLQRAIMSG------GDGGLSEDAESAYVDPERVVEV--GPTRE-- 276
Query: 281 PRYQGMENLSCRACKAKDVSMLLIPCRHLSLCKDCDGFINVCPVCQMIKTASVEVYLS 338
C+ C + S++++PCRHL +C +CD CPVC +K +SV+VYLS
Sbjct: 275 ----------CKGCWKRVASVVVLPCRHLCVCTECDAQFRACPVCLTLKNSSVQVYLS 132
>TC13350 weakly similar to UP|Q9LPJ0 (Q9LPJ0) F6N18.12, partial (17%)
Length = 489
Score = 59.3 bits (142), Expect = 9e-10
Identities = 21/48 (43%), Positives = 33/48 (68%)
Frame = +3
Query: 291 CRACKAKDVSMLLIPCRHLSLCKDCDGFINVCPVCQMIKTASVEVYLS 338
CR C+ + S++++PCRH+ +C +CD CPVC +K ++VEV LS
Sbjct: 60 CRGCERRVASVVVLPCRHMCICAECDAHFRACPVCLTLKNSTVEVLLS 203
>BP059128
Length = 362
Score = 57.0 bits (136), Expect = 4e-09
Identities = 21/48 (43%), Positives = 31/48 (63%)
Frame = -3
Query: 287 ENLSCRACKAKDVSMLLIPCRHLSLCKDCDGFINVCPVCQMIKTASVE 334
E + C++C ++ L +PCRHL+ CK CD + CPVC M K +S+E
Sbjct: 348 EVMVCKSCHSRKSCFLFLPCRHLASCKSCDAILKACPVCGMQKKSSIE 205
>TC16011 similar to UP|Q8LAY3 (Q8LAY3) Inhibitor of apoptosis-like protein,
partial (16%)
Length = 487
Score = 54.3 bits (129), Expect = 3e-08
Identities = 21/45 (46%), Positives = 29/45 (63%)
Frame = +2
Query: 291 CRACKAKDVSMLLIPCRHLSLCKDCDGFINVCPVCQMIKTASVEV 335
CR C + +L++PCRHL LC C ++ CP+C+ KTASV V
Sbjct: 155 CRNCGKDESCVLILPCRHLCLCTVCGSTLHTCPICKSFKTASVHV 289
>TC15750 similar to UP|Q8LAY3 (Q8LAY3) Inhibitor of apoptosis-like protein,
partial (14%)
Length = 547
Score = 53.9 bits (128), Expect = 4e-08
Identities = 24/48 (50%), Positives = 29/48 (60%)
Frame = +3
Query: 291 CRACKAKDVSMLLIPCRHLSLCKDCDGFINVCPVCQMIKTASVEVYLS 338
CR+C K+ +LL+PCRHL LC C + CPVC ASV V LS
Sbjct: 78 CRSCGVKESVVLLLPCRHLCLCTACGSTVRNCPVCDSGMDASVHVNLS 221
>BP030839
Length = 379
Score = 50.8 bits (120), Expect = 3e-07
Identities = 19/48 (39%), Positives = 31/48 (64%)
Frame = -3
Query: 291 CRACKAKDVSMLLIPCRHLSLCKDCDGFINVCPVCQMIKTASVEVYLS 338
CR C ++ +L++PCRHL +C C ++ CP+C+ K ASV V ++
Sbjct: 161 CRKCGKEESCVLILPCRHLCVCTLCGSTLHHCPICKSYKNASVHVNMT 18
>BP051594
Length = 376
Score = 46.6 bits (109), Expect = 6e-06
Identities = 16/37 (43%), Positives = 27/37 (72%)
Frame = -3
Query: 298 DVSMLLIPCRHLSLCKDCDGFINVCPVCQMIKTASVE 334
+V+++L+PC+HL LCKDC+ ++ CP+C K +E
Sbjct: 242 EVTLVLLPCKHLCLCKDCESKLSFCPLCPSSKFIGLE 132
>TC11487 weakly similar to UP|Q8LAY3 (Q8LAY3) Inhibitor of apoptosis-like
protein, partial (13%)
Length = 705
Score = 45.4 bits (106), Expect = 1e-05
Identities = 24/82 (29%), Positives = 51/82 (61%), Gaps = 2/82 (2%)
Frame = +2
Query: 115 SSVTSASGSM--TAPPSIILSLGDNIRTELDRQQEELDHYIKLQKEQLSRGVRDMKQKHM 172
SS T+A+ ++ + S LG++I ++ +QQ ++D+ I + E++ V + +++
Sbjct: 458 SSRTTATAAVNKSCASSSFSFLGEDISLQIHQQQVDIDNLIAQRMEKVRMEVDEKRKRQA 637
Query: 173 ASLLTSIEKGIVKKLREKEEEI 194
L+ ++E G++KKL+ K+EEI
Sbjct: 638 KRLIEAVEIGVMKKLKSKQEEI 703
>AV779203
Length = 487
Score = 44.7 bits (104), Expect = 2e-05
Identities = 20/58 (34%), Positives = 32/58 (54%), Gaps = 2/58 (3%)
Frame = -1
Query: 281 PRYQGMEN--LSCRACKAKDVSMLLIPCRHLSLCKDCDGFINVCPVCQMIKTASVEVY 336
P Y+ ++N + CR C ++S++L+PCRH LC C CP+ + + V VY
Sbjct: 415 PEYERLKNEKVLCRICFEGEISVVLLPCRHRVLCSTCSEKCKKCPISRDSISERVPVY 242
>TC17849
Length = 589
Score = 43.9 bits (102), Expect = 4e-05
Identities = 14/46 (30%), Positives = 29/46 (62%)
Frame = +2
Query: 291 CRACKAKDVSMLLIPCRHLSLCKDCDGFINVCPVCQMIKTASVEVY 336
CR C + +V + ++PC H+ LC+ C ++ CP C++ T ++ ++
Sbjct: 167 CRVCLSAEVDITIVPCGHV-LCRKCSSAVSKCPFCRLQVTKAIRIF 301
>TC8961 similar to GB|BAB71852.1|16902294|AB062739 kinesin-related protein
{Arabidopsis thaliana;} , partial (8%)
Length = 735
Score = 43.1 bits (100), Expect = 7e-05
Identities = 14/36 (38%), Positives = 21/36 (57%)
Frame = +1
Query: 291 CRACKAKDVSMLLIPCRHLSLCKDCDGFINVCPVCQ 326
C+ C + +L+PCRH LCK C + CP+C+
Sbjct: 253 CKVCFESSTAAILLPCRHFCLCKSCSLACSECPICR 360
>BP071727
Length = 391
Score = 39.7 bits (91), Expect = 7e-04
Identities = 13/35 (37%), Positives = 23/35 (65%)
Frame = -1
Query: 292 RACKAKDVSMLLIPCRHLSLCKDCDGFINVCPVCQ 326
R C ++ +L++PCRHL +C C ++ CP+C+
Sbjct: 178 RKCGKEESCVLILPCRHLCVCTLCGSTLHNCPICK 74
>AV766525
Length = 564
Score = 37.4 bits (85), Expect = 0.004
Identities = 11/36 (30%), Positives = 19/36 (52%)
Frame = -1
Query: 291 CRACKAKDVSMLLIPCRHLSLCKDCDGFINVCPVCQ 326
C C K+ + + +PC H+ C C + CP+C+
Sbjct: 414 CVICLEKEYNSVFVPCGHMCCCTGCSSHLTSCPLCR 307
>TC14881
Length = 609
Score = 36.6 bits (83), Expect = 0.006
Identities = 14/52 (26%), Positives = 26/52 (49%), Gaps = 4/52 (7%)
Frame = +3
Query: 291 CRACKAKDVSMLLIPCRHLSLCKDCDGFI----NVCPVCQMIKTASVEVYLS 338
C C + ++PCRH+ +C DC + N CP+C+ +E+ ++
Sbjct: 99 CVICMTEPKDTAVLPCRHMCMCSDCAKELRLQSNKCPICRQPIEELIEIKIN 254
>BP043656
Length = 412
Score = 35.4 bits (80), Expect = 0.014
Identities = 12/35 (34%), Positives = 19/35 (54%)
Frame = -2
Query: 292 RACKAKDVSMLLIPCRHLSLCKDCDGFINVCPVCQ 326
+ C + +L+P RH LCK C + CP+C+
Sbjct: 411 KVCFESPTAAILLPSRHFCLCKSCSLACSECPICR 307
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.315 0.131 0.375
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,345,571
Number of Sequences: 28460
Number of extensions: 68917
Number of successful extensions: 414
Number of sequences better than 10.0: 59
Number of HSP's better than 10.0 without gapping: 410
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 410
length of query: 338
length of database: 4,897,600
effective HSP length: 91
effective length of query: 247
effective length of database: 2,307,740
effective search space: 570011780
effective search space used: 570011780
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 55 (25.8 bits)
Lotus: description of TM0008.8