BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0008.15
(375 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC9042 weakly similar to PIR|G86367|G86367 protein F28C11.3 [imp... 311 9e-86
TC19922 203 3e-66
TC20232 207 3e-54
AV772121 50 6e-07
BG662323 44 3e-05
TC13303 weakly similar to UP|Q9LM55 (Q9LM55) F2E2.11, partial (22%) 33 0.10
TC15699 similar to UP|O64797 (O64797) T1F15.5 protein, partial (... 32 0.22
TC11113 weakly similar to UP|Q9SBM1 (Q9SBM1) Hydroxyproline-rich... 30 0.65
AV418977 30 0.85
TC8721 29 1.5
TC7794 homologue to UP|Q9M600 (Q9M600) Chlorophyll a/b binding p... 28 1.9
TC20019 high affinity ammonium transporter [Lotus corniculatus v... 25 2.0
TC14344 similar to UP|Q40310 (Q40310) Chalcone reductase, complete 28 3.2
TC14345 homologue to UP|Q39774 (Q39774) Polyketide reductase, pa... 27 4.2
TC9353 weakly similar to UP|Q9SM88 (Q9SM88) Zwh21.1, partial (18%) 27 5.5
AV771455 27 5.5
TC16378 similar to GB|AAM70554.1|21700861|AY124845 At1g75220/F22... 27 7.2
BP072816 27 7.2
BP032664 27 7.2
TC15327 26 9.4
>TC9042 weakly similar to PIR|G86367|G86367 protein F28C11.3 [imported] -
Arabidopsis thaliana {Arabidopsis thaliana;}, partial
(12%)
Length = 768
Score = 311 bits (798), Expect = 9e-86
Identities = 146/231 (63%), Positives = 179/231 (77%)
Frame = +2
Query: 10 EAPIHGDVLETILSHVPLIHLVPACHVSNTWKHAVFSSLAHTRTIKPWLIILTQSTRASH 69
EAPIHGD+LE ILSHVPLIHLVPA HVS +WK AV SSL H +KPWL + TQS RA H
Sbjct: 86 EAPIHGDILEAILSHVPLIHLVPARHVSKSWKRAVSSSLRHVSPVKPWLTVHTQSPRAPH 265
Query: 70 VITAHAYDPRSHVWVEMKLPHPLKGHPSATAAVRSSHSTLLYTLSPAEFAFSIDALHLDW 129
V T+HAYDPRS W+++ P + +++ +RSSHSTL+YTLSP F+FS+D L L+W
Sbjct: 266 VTTSHAYDPRSRSWIQIHAPPSSQ----SSSPLRSSHSTLIYTLSPTGFSFSLDPLGLEW 433
Query: 130 HHAPAPRVWRTDPIVARVGQRVVVAGGACDFEDDPLAVEMYDMESNTWVMCQSMPAMLKG 189
HHAP PRVWR DP+VA VG ++VAGGACDFEDDPLAVEMYD S TW C+SMP +LKG
Sbjct: 434 HHAPPPRVWRVDPVVACVGGCIIVAGGACDFEDDPLAVEMYDAGSRTWSRCESMPEILKG 613
Query: 190 SSGSTWLSVAVAGEEVLVTEESSGMTFCFDTVSMKWDGPYDLCPDQSVGYC 240
S+ STWLSVAVAG+ V VTE++SG+T+ FD+ + W GPY+L P++ V YC
Sbjct: 614 STASTWLSVAVAGDRVHVTEKNSGVTYSFDSETKSWLGPYELRPEEGVFYC 766
Score = 28.1 bits (61), Expect = 2.5
Identities = 27/84 (32%), Positives = 36/84 (42%), Gaps = 4/84 (4%)
Frame = -3
Query: 66 RASHVITAHAY-DPRSHVWVEMKLPHPLKGHPSATAAVRSSHSTLLYTLSPAEFAFSIDA 124
R H +T Y +PR L H L PSA + V + + L A A + DA
Sbjct: 670 RHVHPVTGDGYREPRRRRGAFQDLRHGLATGPSAGSRVVHLNRQGV-VLEVARAAGNDDA 494
Query: 125 LHL---DWHHAPAPRVWRTDPIVA 145
DW ++P PR WR P+ A
Sbjct: 493 SANTGDDWVNSPHPRGWRVVPLEA 422
>TC19922
Length = 435
Score = 203 bits (516), Expect(3) = 3e-66
Identities = 95/96 (98%), Positives = 95/96 (98%)
Frame = +3
Query: 1 MAAQWELPEEAPIHGDVLETILSHVPLIHLVPACHVSNTWKHAVFSSLAHTRTIKPWLII 60
MAAQWELPEEAPIHGDVLETILSHVPLIHLVPACHVSNTWKHAVFSSLAHTRTIKPWLII
Sbjct: 51 MAAQWELPEEAPIHGDVLETILSHVPLIHLVPACHVSNTWKHAVFSSLAHTRTIKPWLII 230
Query: 61 LTQSTRASHVITAHAYDPRSHVWVEMKLPHPLKGHP 96
LTQSTRASHVITAHAYDPRSHVWVEMKLPHPLKG P
Sbjct: 231 LTQSTRASHVITAHAYDPRSHVWVEMKLPHPLKGTP 338
Score = 50.4 bits (119), Expect(3) = 3e-66
Identities = 23/25 (92%), Positives = 24/25 (96%)
Frame = +2
Query: 91 PLKGHPSATAAVRSSHSTLLYTLSP 115
P +GHPSATAAVRSSHSTLLYTLSP
Sbjct: 320 PFEGHPSATAAVRSSHSTLLYTLSP 394
Score = 36.6 bits (83), Expect(3) = 3e-66
Identities = 15/15 (100%), Positives = 15/15 (100%)
Frame = +1
Query: 115 PAEFAFSIDALHLDW 129
PAEFAFSIDALHLDW
Sbjct: 391 PAEFAFSIDALHLDW 435
>TC20232
Length = 601
Score = 207 bits (526), Expect = 3e-54
Identities = 100/100 (100%), Positives = 100/100 (100%)
Frame = +3
Query: 237 VGYCVTGMLGKRLMVAGVVGDGEDVKAVKLWAVKGGLGSGMTEEVGEMPEELLEKFKGDS 296
VGYCVTGMLGKRLMVAGVVGDGEDVKAVKLWAVKGGLGSGMTEEVGEMPEELLEKFKGDS
Sbjct: 3 VGYCVTGMLGKRLMVAGVVGDGEDVKAVKLWAVKGGLGSGMTEEVGEMPEELLEKFKGDS 182
Query: 297 DELGSLEVTWVGNFVYLRNTLEIEELVMCEVVNGSRCEWR 336
DELGSLEVTWVGNFVYLRNTLEIEELVMCEVVNGSRCEWR
Sbjct: 183 DELGSLEVTWVGNFVYLRNTLEIEELVMCEVVNGSRCEWR 302
>AV772121
Length = 504
Score = 50.1 bits (118), Expect = 6e-07
Identities = 25/33 (75%), Positives = 25/33 (75%), Gaps = 1/33 (3%)
Frame = -3
Query: 323 VMCEVVNGSRCEWRSVRNAAVD-GGTRMVVCGS 354
VMCEVVNGSRCEWRSVR A RMVVCGS
Sbjct: 502 VMCEVVNGSRCEWRSVRYAPCGLAAPRMVVCGS 404
>BG662323
Length = 375
Score = 44.3 bits (103), Expect = 3e-05
Identities = 25/80 (31%), Positives = 37/80 (46%), Gaps = 5/80 (6%)
Frame = +1
Query: 15 GDVLETILSHVPLIHLVPACHVSNTWKHAV-----FSSLAHTRTIKPWLIILTQSTRASH 69
GD+LE IL ++P+ + AC V W+ V S+L+H KPW + T
Sbjct: 82 GDLLEHILMYLPIASIFRACCVCKRWREIVTKERFLSNLSHILPQKPWYFMFTNCDEP-- 255
Query: 70 VITAHAYDPRSHVWVEMKLP 89
+A+DP W +LP
Sbjct: 256 --IGYAFDPFLRKWYGFELP 309
>TC13303 weakly similar to UP|Q9LM55 (Q9LM55) F2E2.11, partial (22%)
Length = 541
Score = 32.7 bits (73), Expect = 0.10
Identities = 24/90 (26%), Positives = 41/90 (44%), Gaps = 6/90 (6%)
Frame = +2
Query: 7 LPEEAPIHGDVLETILSHVPLIHLVPACHVSNTWKHAVFSSLAHT-----RTIKPWLIIL 61
LP+E + I++ +P I + VS+ WK + SS + T + WL +L
Sbjct: 179 LPDELSLQ------IIARLPRICYLNVKLVSDKWKSTITSSELYKVRKELGTTEEWLYLL 340
Query: 62 TQSTRASHVITAHAYDPRSHVWVEM-KLPH 90
+ + + H DPRS +W + +PH
Sbjct: 341 VKV--GENKLMWHGLDPRSQIWQRLPNMPH 424
>TC15699 similar to UP|O64797 (O64797) T1F15.5 protein, partial (53%)
Length = 902
Score = 31.6 bits (70), Expect = 0.22
Identities = 16/55 (29%), Positives = 25/55 (45%)
Frame = +1
Query: 129 WHHAPAPRVWRTDPIVARVGQRVVVAGGACDFEDDPLAVEMYDMESNTWVMCQSM 183
W P V R D A V V GG + D + E+YD +++ W + +S+
Sbjct: 538 WTRLPNMNVARYDFACAEVNGLVYAVGGYGEHGDSLSSAEVYDPDTDKWTLIESL 702
>TC11113 weakly similar to UP|Q9SBM1 (Q9SBM1) Hydroxyproline-rich
glycoprotein DZ-HRGP precursor, partial (10%)
Length = 309
Score = 30.0 bits (66), Expect = 0.65
Identities = 20/68 (29%), Positives = 28/68 (40%), Gaps = 1/68 (1%)
Frame = +1
Query: 69 HVITAHAYD-PRSHVWVEMKLPHPLKGHPSATAAVRSSHSTLLYTLSPAEFAFSIDALHL 127
H +H++ SH W+ + PL+ P +T AV HS L Y S
Sbjct: 22 HFPHSHSHSHSHSHPWLPFPISAPLRSQPPSTPAVH--HSPLPYLPPSPSLPNSPPPPPR 195
Query: 128 DWHHAPAP 135
HH P+P
Sbjct: 196 SGHHHPSP 219
>AV418977
Length = 415
Score = 29.6 bits (65), Expect = 0.85
Identities = 33/123 (26%), Positives = 53/123 (42%), Gaps = 2/123 (1%)
Frame = +2
Query: 165 LAVEMYDMESNTWVMCQSMPAMLKGSSGSTWLSVAVAGEEVLVTEESSG-MTFCFDTVSM 223
L E+YD ++++W + Q MPA SS + + L T G M + DT
Sbjct: 5 LPTEVYDSKADSWSVHQMMPAANLCSSKMAYCDSRL----YLETLSPLGLMMYRLDT--D 166
Query: 224 KWDG-PYDLCPDQSVGYCVTGMLGKRLMVAGVVGDGEDVKAVKLWAVKGGLGSGMTEEVG 282
W+ P GY V G KRL + G +G ++++++W + M E+
Sbjct: 167 HWEHIPAKFPRSLLDGYLVAG-TQKRLFLVGRIGLYSTLQSMRIWELDH--AKSMWVEIS 337
Query: 283 EMP 285
MP
Sbjct: 338 RMP 346
>TC8721
Length = 670
Score = 28.9 bits (63), Expect = 1.5
Identities = 15/43 (34%), Positives = 19/43 (43%)
Frame = -3
Query: 23 SHVPLIHLVPACHVSNTWKHAVFSSLAHTRTIKPWLIILTQST 65
S V H ACH SSL+H +T PW ++ Q T
Sbjct: 152 STVSFFHATDACHT--------LSSLSHMKTTPPWSLVSYQLT 48
>TC7794 homologue to UP|Q9M600 (Q9M600) Chlorophyll a/b binding protein
precursor, partial (97%)
Length = 2581
Score = 28.5 bits (62), Expect = 1.9
Identities = 20/47 (42%), Positives = 22/47 (46%), Gaps = 10/47 (21%)
Frame = -3
Query: 227 GPYDLCPDQSVGYCVTGM-------LGKR---LMVAGVVGDGEDVKA 263
GPY L S G TG L KR L VAG+ GDG D+ A
Sbjct: 1715 GPYHLASSSSAGATATGFCFGAGFFLEKRATVLKVAGLEGDGADLVA 1575
>TC20019 high affinity ammonium transporter [Lotus corniculatus var.
japonicus]
Length = 1521
Score = 25.0 bits (53), Expect(2) = 2.0
Identities = 11/30 (36%), Positives = 19/30 (62%)
Frame = -1
Query: 45 FSSLAHTRTIKPWLIILTQSTRASHVITAH 74
F S H + ++I+++QST SH+ T+H
Sbjct: 1467 FQSFLHRPHV--FMILISQSTMTSHIQTSH 1384
Score = 21.6 bits (44), Expect(2) = 2.0
Identities = 10/26 (38%), Positives = 11/26 (41%)
Frame = -3
Query: 81 HVWVEMKLPHPLKGHPSATAAVRSSH 106
HV PHPLK H + A H
Sbjct: 1279 HVQPIASNPHPLKAHMAYQEAQHKPH 1202
>TC14344 similar to UP|Q40310 (Q40310) Chalcone reductase, complete
Length = 1180
Score = 27.7 bits (60), Expect = 3.2
Identities = 15/47 (31%), Positives = 21/47 (43%)
Frame = -2
Query: 8 PEEAPIHGDVLETILSHVPLIHLVPACHVSNTWKHAVFSSLAHTRTI 54
PE H + H P +HLVP CH+S+ + H RT+
Sbjct: 819 PEL*SFHEPLQSLSTYHSP*LHLVPCCHLSSKVRRR*GLFHLHCRTL 679
>TC14345 homologue to UP|Q39774 (Q39774) Polyketide reductase, partial (35%)
Length = 516
Score = 27.3 bits (59), Expect = 4.2
Identities = 14/47 (29%), Positives = 21/47 (43%)
Frame = -1
Query: 8 PEEAPIHGDVLETILSHVPLIHLVPACHVSNTWKHAVFSSLAHTRTI 54
PE H + H P +HL+P CH+S+ + H RT+
Sbjct: 144 PEL*TFHEPLQSLSTYHSP*LHLLPCCHLSSKVRRRSGLFHLHCRTL 4
>TC9353 weakly similar to UP|Q9SM88 (Q9SM88) Zwh21.1, partial (18%)
Length = 1149
Score = 26.9 bits (58), Expect = 5.5
Identities = 17/60 (28%), Positives = 27/60 (44%)
Frame = +1
Query: 153 VAGGACDFEDDPLAVEMYDMESNTWVMCQSMPAMLKGSSGSTWLSVAVAGEEVLVTEESS 212
V GG + D E YD ++N+W + M + S + +AVA E+ + SS
Sbjct: 184 VIGGQDENNKDLTCGEFYDGKTNSWSLIPDMLKDIPPSVSQSPPLIAVASNELYSLDASS 363
>AV771455
Length = 454
Score = 26.9 bits (58), Expect = 5.5
Identities = 10/24 (41%), Positives = 14/24 (57%)
Frame = -3
Query: 156 GACDFEDDPLAVEMYDMESNTWVM 179
GAC+FE + +E+Y N W M
Sbjct: 173 GACNFESVVIIIELYVKYMNVWCM 102
>TC16378 similar to GB|AAM70554.1|21700861|AY124845 At1g75220/F22H5_6
{Arabidopsis thaliana;}, partial (31%)
Length = 738
Score = 26.6 bits (57), Expect = 7.2
Identities = 39/135 (28%), Positives = 57/135 (41%), Gaps = 11/135 (8%)
Frame = +2
Query: 98 ATAAVRSSHSTLLYTLSPAEFAFSID----ALHLDWHHAPAPRVWRTDPIVARVGQRVVV 153
+T V S LL LS + S+ A +L+ + +++ IV+ VG V+V
Sbjct: 2 STWLVDKSGRRLLLLLSSSIMTVSLVVVSIAFYLEGTVSEDSHLYKILGIVSVVGLVVMV 181
Query: 154 AGGACDFEDDPLAVEMYDMESNTWV-MCQSMPAMLKGSSGST-----WL-SVAVAGEEVL 206
G + P WV M + +P +KG +GST WL S A+ L
Sbjct: 182 IGFSLGLGPIP------------WVIMSEILPVSIKGLAGSTATMANWLTSWAITMTANL 325
Query: 207 VTEESSGMTFCFDTV 221
+ SSG TF TV
Sbjct: 326 LLTWSSGGTFTMYTV 370
>BP072816
Length = 492
Score = 26.6 bits (57), Expect = 7.2
Identities = 15/35 (42%), Positives = 16/35 (44%)
Frame = -2
Query: 252 AGVVGDGEDVKAVKLWAVKGGLGSGMTEEVGEMPE 286
A V ED W+V GG GSG EE E E
Sbjct: 215 AAVADGAEDGDVEGAWSVVGGEGSGEEEEEDEEGE 111
>BP032664
Length = 561
Score = 26.6 bits (57), Expect = 7.2
Identities = 22/66 (33%), Positives = 26/66 (39%), Gaps = 16/66 (24%)
Frame = +3
Query: 168 EMYDMESNTWVMCQ---------SMPAMLKGSSGSTWLSVAVAGE-------EVLVTEES 211
EM DME + W M Q P + G + + WL V A E EV S
Sbjct: 141 EMRDMEEDEWQMVQKKGSQFVVNDQPFYINGFN-TYWLMVFAADESTRGKVTEVFKQASS 317
Query: 212 SGMTFC 217
GMT C
Sbjct: 318 VGMTVC 335
>TC15327
Length = 485
Score = 26.2 bits (56), Expect = 9.4
Identities = 13/27 (48%), Positives = 16/27 (59%)
Frame = +2
Query: 339 RNAAVDGGTRMVVCGSEVRLEDLQSAM 365
RN V RMV CG V +EDL+ A+
Sbjct: 2 RNEXVARMERMVFCGGYVGVEDLECAV 82
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.319 0.133 0.418
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,628,252
Number of Sequences: 28460
Number of extensions: 113508
Number of successful extensions: 599
Number of sequences better than 10.0: 46
Number of HSP's better than 10.0 without gapping: 593
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 596
length of query: 375
length of database: 4,897,600
effective HSP length: 92
effective length of query: 283
effective length of database: 2,279,280
effective search space: 645036240
effective search space used: 645036240
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 56 (26.2 bits)
Lotus: description of TM0008.15