BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0008.14
(423 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC14458 homologue to UP|Q93YH3 (Q93YH3) ATP citrate lyase b-subu... 581 e-167
TC14459 homologue to UP|Q93YH3 (Q93YH3) ATP citrate lyase b-subu... 385 e-108
TC16405 similar to UP|Q93YH3 (Q93YH3) ATP citrate lyase b-subuni... 284 2e-77
TC10735 homologue to UP|Q93YH3 (Q93YH3) ATP citrate lyase b-subu... 265 1e-71
AV776142 167 2e-42
TC10096 homologue to UP|Q93YH3 (Q93YH3) ATP citrate lyase b-subu... 152 1e-37
TC14255 28 2.9
BP041939 27 4.9
TC13445 similar to UP|Q84P18 (Q84P18) Acyl-activating enzyme 17,... 27 4.9
BP051648 27 6.4
TC12867 similar to GB|AAL91627.1|19715609|AY075613 AT3g59630/T16... 27 8.3
>TC14458 homologue to UP|Q93YH3 (Q93YH3) ATP citrate lyase b-subunit ,
partial (69%)
Length = 1120
Score = 581 bits (1498), Expect = e-167
Identities = 290/290 (100%), Positives = 290/290 (100%)
Frame = +1
Query: 134 ECGGIDIEENWDKVKTVFIPTGVSLTSEIVAPLVATLPLEIKGEIEEFLKVIFTLFQDLD 193
ECGGIDIEENWDKVKTVFIPTGVSLTSEIVAPLVATLPLEIKGEIEEFLKVIFTLFQDLD
Sbjct: 4 ECGGIDIEENWDKVKTVFIPTGVSLTSEIVAPLVATLPLEIKGEIEEFLKVIFTLFQDLD 183
Query: 194 FTFLEMNPFTLVDGKPCPLDMRGELDDTAAFKNFKKWGNIEFPLPFGRVMSATESFVHEL 253
FTFLEMNPFTLVDGKPCPLDMRGELDDTAAFKNFKKWGNIEFPLPFGRVMSATESFVHEL
Sbjct: 184 FTFLEMNPFTLVDGKPCPLDMRGELDDTAAFKNFKKWGNIEFPLPFGRVMSATESFVHEL 363
Query: 254 DEKTSASLKFTVLNPKGRIWTMVAGGGASVIYADTVGDLGFASELGNYAEYSGAPKEDEV 313
DEKTSASLKFTVLNPKGRIWTMVAGGGASVIYADTVGDLGFASELGNYAEYSGAPKEDEV
Sbjct: 364 DEKTSASLKFTVLNPKGRIWTMVAGGGASVIYADTVGDLGFASELGNYAEYSGAPKEDEV 543
Query: 314 LQYARVVIDCATANPDGQKRALVIGGGIANFTDVAATFSGIIRALKEKESKLKAARMHIY 373
LQYARVVIDCATANPDGQKRALVIGGGIANFTDVAATFSGIIRALKEKESKLKAARMHIY
Sbjct: 544 LQYARVVIDCATANPDGQKRALVIGGGIANFTDVAATFSGIIRALKEKESKLKAARMHIY 723
Query: 374 VRRGGPNYQKGLALMRALGEEIGIPIEVYGPEATMTGICKEAIQCITAAA 423
VRRGGPNYQKGLALMRALGEEIGIPIEVYGPEATMTGICKEAIQCITAAA
Sbjct: 724 VRRGGPNYQKGLALMRALGEEIGIPIEVYGPEATMTGICKEAIQCITAAA 873
>TC14459 homologue to UP|Q93YH3 (Q93YH3) ATP citrate lyase b-subunit ,
partial (48%)
Length = 777
Score = 385 bits (989), Expect = e-108
Identities = 189/202 (93%), Positives = 197/202 (96%)
Frame = +2
Query: 222 AAFKNFKKWGNIEFPLPFGRVMSATESFVHELDEKTSASLKFTVLNPKGRIWTMVAGGGA 281
AAFKNFKKWGNIEFPLPFGRVMS TE+F+H LDEKTSASLKFTVLNP+GRIWTMVAGGGA
Sbjct: 2 AAFKNFKKWGNIEFPLPFGRVMSPTETFIHGLDEKTSASLKFTVLNPEGRIWTMVAGGGA 181
Query: 282 SVIYADTVGDLGFASELGNYAEYSGAPKEDEVLQYARVVIDCATANPDGQKRALVIGGGI 341
SVIYADTVGDLG+A+ELGNYAEYSGAPKEDEVLQYARVVIDCATANPDGQKRALVIGGGI
Sbjct: 182 SVIYADTVGDLGYAAELGNYAEYSGAPKEDEVLQYARVVIDCATANPDGQKRALVIGGGI 361
Query: 342 ANFTDVAATFSGIIRALKEKESKLKAARMHIYVRRGGPNYQKGLALMRALGEEIGIPIEV 401
ANFTDVAATFSGIIRALKEKE KLK A+MH+YVRRGGPNYQKGLA MRALGEEIGIPIEV
Sbjct: 362 ANFTDVAATFSGIIRALKEKEQKLKEAKMHLYVRRGGPNYQKGLAKMRALGEEIGIPIEV 541
Query: 402 YGPEATMTGICKEAIQCITAAA 423
YGPEATMTGICK+AIQCITAAA
Sbjct: 542 YGPEATMTGICKQAIQCITAAA 607
>TC16405 similar to UP|Q93YH3 (Q93YH3) ATP citrate lyase b-subunit ,
partial (42%)
Length = 597
Score = 284 bits (727), Expect = 2e-77
Identities = 138/182 (75%), Positives = 161/182 (87%)
Frame = +3
Query: 1 MARKKIREYDSKRLVKEHFKRLSGKELPIKSAQVTGSTDFSELQDKEPWLSSSKLVVKPD 60
MARKKIREY SK+L+KEH KRL+ +L IKSAQVT STDF+EL ++ PWLSS+KLVVKPD
Sbjct: 51 MARKKIREYHSKKLLKEHLKRLASIDLDIKSAQVTESTDFAELTNQHPWLSSTKLVVKPD 230
Query: 61 MLFGKRGKSGLVALNLDFAQVASFVKERLGKEVEMGGCKGPITTFIVEPFIPHNEEFYLN 120
MLFGKRGKSGLVALNLD AQVA FVKERLG EVEMGGCK PITTFIVEPF+PH EFYL+
Sbjct: 231 MLFGKRGKSGLVALNLDVAQVAEFVKERLGVEVEMGGCKAPITTFIVEPFVPHELEFYLS 410
Query: 121 IVSERLGNSISFSECGGIDIEENWDKVKTVFIPTGVSLTSEIVAPLVATLPLEIKGEIEE 180
+VSERLG++ISFSECGGI+IEENWDKVKT+F+PT +T E APL+ATLPLE++G+I +
Sbjct: 411 VVSERLGSTISFSECGGIEIEENWDKVKTIFLPTEKPMTLEACAPLIATLPLEVRGKIGD 590
Query: 181 FL 182
F+
Sbjct: 591 FI 596
>TC10735 homologue to UP|Q93YH3 (Q93YH3) ATP citrate lyase b-subunit ,
partial (31%)
Length = 510
Score = 265 bits (676), Expect = 1e-71
Identities = 133/133 (100%), Positives = 133/133 (100%)
Frame = +3
Query: 1 MARKKIREYDSKRLVKEHFKRLSGKELPIKSAQVTGSTDFSELQDKEPWLSSSKLVVKPD 60
MARKKIREYDSKRLVKEHFKRLSGKELPIKSAQVTGSTDFSELQDKEPWLSSSKLVVKPD
Sbjct: 111 MARKKIREYDSKRLVKEHFKRLSGKELPIKSAQVTGSTDFSELQDKEPWLSSSKLVVKPD 290
Query: 61 MLFGKRGKSGLVALNLDFAQVASFVKERLGKEVEMGGCKGPITTFIVEPFIPHNEEFYLN 120
MLFGKRGKSGLVALNLDFAQVASFVKERLGKEVEMGGCKGPITTFIVEPFIPHNEEFYLN
Sbjct: 291 MLFGKRGKSGLVALNLDFAQVASFVKERLGKEVEMGGCKGPITTFIVEPFIPHNEEFYLN 470
Query: 121 IVSERLGNSISFS 133
IVSERLGNSISFS
Sbjct: 471 IVSERLGNSISFS 509
>AV776142
Length = 366
Score = 167 bits (424), Expect = 2e-42
Identities = 86/97 (88%), Positives = 90/97 (92%)
Frame = +3
Query: 1 MARKKIREYDSKRLVKEHFKRLSGKELPIKSAQVTGSTDFSELQDKEPWLSSSKLVVKPD 60
MARKKIREYDSKRL+KEH KRLSG+ELPIKSAQVT STDF+EL KEPWLSSSKLVVKPD
Sbjct: 75 MARKKIREYDSKRLLKEHLKRLSGQELPIKSAQVTESTDFNELAGKEPWLSSSKLVVKPD 254
Query: 61 MLFGKRGKSGLVALNLDFAQVASFVKERLGKEVEMGG 97
ML+GKRGKSGLVALNLD A VAS VKERLGKEVEMGG
Sbjct: 255 MLYGKRGKSGLVALNLD*ADVASLVKERLGKEVEMGG 365
>TC10096 homologue to UP|Q93YH3 (Q93YH3) ATP citrate lyase b-subunit ,
partial (19%)
Length = 484
Score = 152 bits (383), Expect = 1e-37
Identities = 74/84 (88%), Positives = 79/84 (93%)
Frame = +3
Query: 340 GIANFTDVAATFSGIIRALKEKESKLKAARMHIYVRRGGPNYQKGLALMRALGEEIGIPI 399
GIANFTDVAATFSGIIRALKEKESKLKAARMHI+VRRGGPNYQ GLA MRALGEE+G+PI
Sbjct: 15 GIANFTDVAATFSGIIRALKEKESKLKAARMHIFVRRGGPNYQTGLAKMRALGEELGVPI 194
Query: 400 EVYGPEATMTGICKEAIQCITAAA 423
EVYGPEATMTGICK+AI CI + A
Sbjct: 195 EVYGPEATMTGICKQAIDCIMSEA 266
>TC14255
Length = 641
Score = 28.1 bits (61), Expect = 2.9
Identities = 16/37 (43%), Positives = 20/37 (53%)
Frame = -3
Query: 263 FTVLNPKGRIWTMVAGGGASVIYADTVGDLGFASELG 299
FTV N G IW +GGG +T G+ G +ELG
Sbjct: 396 FTVGNESG-IWQSQSGGGDGETAGETEGESGGFNELG 289
>BP041939
Length = 392
Score = 27.3 bits (59), Expect = 4.9
Identities = 14/36 (38%), Positives = 20/36 (54%), Gaps = 1/36 (2%)
Frame = -3
Query: 366 KAARMHIYVRR-GGPNYQKGLALMRALGEEIGIPIE 400
K ++YV+ GGPNY +GL + EI P+E
Sbjct: 339 KHGTFYLYVKL*GGPNYHEGLKTPEEVKVEIEYPME 232
>TC13445 similar to UP|Q84P18 (Q84P18) Acyl-activating enzyme 17, partial
(13%)
Length = 515
Score = 27.3 bits (59), Expect = 4.9
Identities = 23/70 (32%), Positives = 32/70 (44%), Gaps = 1/70 (1%)
Frame = -3
Query: 87 ERLGKEVEMGGCKGPITTFI-VEPFIPHNEEFYLNIVSERLGNSISFSECGGIDIEENWD 145
ER GKE K PIT+F + F N E Y V E + +ISFS+ + +N
Sbjct: 219 ERRGKEFLGSAYKEPITSFSDFQKFSVSNPEVYWETVFEEM--NISFSKPPECILRDNLP 46
Query: 146 KVKTVFIPTG 155
+P+G
Sbjct: 45 GEDRSSLPSG 16
>BP051648
Length = 558
Score = 26.9 bits (58), Expect = 6.4
Identities = 11/35 (31%), Positives = 19/35 (53%)
Frame = -2
Query: 97 GCKGPITTFIVEPFIPHNEEFYLNIVSERLGNSIS 131
GC G + + EPF+ N + ++ ++ GNS S
Sbjct: 164 GCHGGLKVPVKEPFLTINSDIFVLFLNHPFGNSPS 60
>TC12867 similar to GB|AAL91627.1|19715609|AY075613 AT3g59630/T16L24_180
{Arabidopsis thaliana;}, partial (16%)
Length = 788
Score = 26.6 bits (57), Expect = 8.3
Identities = 17/39 (43%), Positives = 20/39 (50%), Gaps = 5/39 (12%)
Frame = +2
Query: 121 IVSERLGNSISFSECG----GIDIEENWDKVKTV-FIPT 154
I S R GNS+ F CG +D E N+D T FI T
Sbjct: 278 IYSNRKGNSLFFIACGLQPMAMDFESNYDIAATAHFIRT 394
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.318 0.138 0.399
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,228,828
Number of Sequences: 28460
Number of extensions: 74964
Number of successful extensions: 307
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 307
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 307
length of query: 423
length of database: 4,897,600
effective HSP length: 93
effective length of query: 330
effective length of database: 2,250,820
effective search space: 742770600
effective search space used: 742770600
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 56 (26.2 bits)
Lotus: description of TM0008.14