BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0005.12
(526 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC18897 weakly similar to UP|Q9XEV3 (Q9XEV3) TIA-1 related prote... 73 1e-13
BP078889 68 4e-12
BP051903 64 8e-11
BG662008 62 2e-10
AV417455 41 6e-04
AV417593 40 0.001
TC12113 weakly similar to GB|CAD40580.1|21741678|OSJN00141 oj000... 37 0.010
TC9728 similar to UP|Q94F45 (Q94F45) AT4g18020/T6K21_200, partia... 37 0.010
TC8015 similar to UP|ROC4_NICSY (P19683) 31 kDa ribonucleoprotei... 33 0.088
TC8296 similar to GB|AAN28790.1|23505861|AY143851 At5g64200/MSJ1... 33 0.11
TC12416 similar to GB|AAH56066.1|33417132|BC056066 sop-prov prot... 33 0.11
TC8576 similar to UP|Q9SJN3 (Q9SJN3) At2g36220 protein (At2g3622... 33 0.15
AI967582 32 0.26
TC16529 similar to UP|Q9M549 (Q9M549) Poly(A)-binding protein, p... 32 0.33
BI418438 32 0.33
TC13226 similar to UP|HMGB_SOYBN (Q10370) HMG-Y related protein ... 31 0.57
TC14530 similar to UP|P82277 (P82277) Plastid-specific 30S ribos... 31 0.57
BP081080 31 0.57
AV773507 31 0.57
TC11683 similar to UP|Q94AC3 (Q94AC3) AT4g36960/C7A10_400, parti... 30 0.97
>TC18897 weakly similar to UP|Q9XEV3 (Q9XEV3) TIA-1 related protein, partial
(5%)
Length = 481
Score = 73.2 bits (178), Expect = 1e-13
Identities = 40/96 (41%), Positives = 52/96 (53%)
Frame = +1
Query: 22 SRQAAGGTFGDLSSVDDEGDDEGEQDGGDARVARVVAPPARTCFPLFVDGIADSVNYFHV 81
S G D ++DE +E GG A F LF+DGI++ NY+H+
Sbjct: 181 SVSGCSGVDSDEDLLEDEAGEEPAIGGGRA-----------PSFTLFIDGISEPTNYYHI 327
Query: 82 RDLFLQHGKLTNVFLQRKRNRGRKFRFGFVRYANRE 117
R LF Q G L NVFLQ++R R FRFGFVRY+ R+
Sbjct: 328 RGLFGQIGSLLNVFLQKQRRIRRNFRFGFVRYSTRD 435
>BP078889
Length = 474
Score = 67.8 bits (164), Expect = 4e-12
Identities = 37/98 (37%), Positives = 54/98 (54%)
Frame = +1
Query: 20 ITSRQAAGGTFGDLSSVDDEGDDEGEQDGGDARVARVVAPPARTCFPLFVDGIADSVNYF 79
+ S A GG DL ++ D+GE + R R FVDGI +++ Y
Sbjct: 106 VESEGALGGPIRDLGG-EEVASDKGETRNDAGGLNRNNGGERRRKPTPFVDGIEEAIEYH 282
Query: 80 HVRDLFLQHGKLTNVFLQRKRNRGRKFRFGFVRYANRE 117
H+R LF G+++N+F+QRK GR+FRFGFVR+ + E
Sbjct: 283 HLRGLFALFGRISNLFVQRKHKFGRRFRFGFVRFFSEE 396
>BP051903
Length = 342
Score = 63.5 bits (153), Expect = 8e-11
Identities = 29/53 (54%), Positives = 38/53 (70%)
Frame = +2
Query: 69 VDGIADSVNYFHVRDLFLQHGKLTNVFLQRKRNRGRKFRFGFVRYANREDVMK 121
VDGI D +Y+ +R LF Q G++ NVF+QR+R GR FRFGFVR+A+ E K
Sbjct: 68 VDGIPDRTDYYQIRGLFAQFGRVVNVFVQRQRKIGRLFRFGFVRFASLEVASK 226
>BG662008
Length = 457
Score = 62.0 bits (149), Expect = 2e-10
Identities = 30/51 (58%), Positives = 37/51 (71%)
Frame = +3
Query: 67 LFVDGIADSVNYFHVRDLFLQHGKLTNVFLQRKRNRGRKFRFGFVRYANRE 117
LFVDGI+D V+Y +R LF G L NVF+QR+R GR FRFGFVR+ + E
Sbjct: 105 LFVDGISDFVDYSQIRGLFSHIGSLLNVFVQRQRRIGRAFRFGFVRFPSVE 257
>AV417455
Length = 261
Score = 40.8 bits (94), Expect = 6e-04
Identities = 22/50 (44%), Positives = 27/50 (54%), Gaps = 1/50 (2%)
Frame = +3
Query: 157 SRSWCDVLKGCSAKWIEKQPLLDSEVMLMGVGGCVGLN-RISDRILFPEL 205
SRSW DV+KG S + D + ML G G C R +D +LFPEL
Sbjct: 108 SRSWSDVVKGISKECSSVGAGFDCDAMLAGAGFCCSREVRFADEVLFPEL 257
>AV417593
Length = 423
Score = 40.0 bits (92), Expect = 0.001
Identities = 20/54 (37%), Positives = 29/54 (53%)
Frame = +2
Query: 67 LFVDGIADSVNYFHVRDLFLQHGKLTNVFLQRKRNRGRKFRFGFVRYANREDVM 120
++V + +V VR LF HGK+T V L ++ K R GFV +A R + M
Sbjct: 260 VYVKNLPKNVTQEQVRKLFEHHGKITKVVLPPPKSGQEKNRIGFVHFAERSNAM 421
>TC12113 weakly similar to GB|CAD40580.1|21741678|OSJN00141 oj000126_13.2
{Oryza sativa (japonica cultivar-group);} , partial
(40%)
Length = 484
Score = 36.6 bits (83), Expect = 0.010
Identities = 26/79 (32%), Positives = 36/79 (44%), Gaps = 8/79 (10%)
Frame = +1
Query: 71 GIADSVNYFHVRDLFLQHGKLTNVFLQRKRNRGRKFRFGFVRYANREDVMK---GRSRK- 126
G+A S + + F Q+GK+ + R + R FG+V +A ED K G +RK
Sbjct: 19 GLAFSTTEDKLAEAFSQYGKVLKADIVLNRAKTRSKGFGYVTFAKEEDAQKAQIGMNRKI 198
Query: 127 ----QSYVSMAPPTTVKDS 141
YV M PP K S
Sbjct: 199 LHGRVLYVDMEPPDQHKKS 255
>TC9728 similar to UP|Q94F45 (Q94F45) AT4g18020/T6K21_200, partial (5%)
Length = 636
Score = 36.6 bits (83), Expect = 0.010
Identities = 18/34 (52%), Positives = 22/34 (63%)
Frame = +2
Query: 327 SNFLPWLNDYLMLLSIPFSAYPVGSVLAAPRQVF 360
SN LPW+ L LS P AYP S+LAAPR ++
Sbjct: 23 SNLLPWI---LSCLSSPAKAYPASSLLAAPRALY 115
>TC8015 similar to UP|ROC4_NICSY (P19683) 31 kDa ribonucleoprotein,
chloroplast precursor, partial (28%)
Length = 717
Score = 33.5 bits (75), Expect = 0.088
Identities = 15/53 (28%), Positives = 28/53 (52%)
Frame = +3
Query: 67 LFVDGIADSVNYFHVRDLFLQHGKLTNVFLQRKRNRGRKFRFGFVRYANREDV 119
++V + SV+ + ++F +HG + N + R GR FGFV ++ D+
Sbjct: 84 VYVGNLPWSVDTARLEEIFREHGNVENARIVMDRETGRSRGFGFVTMSSEADI 242
>TC8296 similar to GB|AAN28790.1|23505861|AY143851 At5g64200/MSJ1_4
{Arabidopsis thaliana;}, partial (55%)
Length = 1276
Score = 33.1 bits (74), Expect = 0.11
Identities = 21/79 (26%), Positives = 36/79 (44%), Gaps = 3/79 (3%)
Frame = +2
Query: 84 LFLQHGKLTNVFLQRKRNRGRKFRFGFVRYANREDVMKGRSRKQSYVSMAPPTTVKDSR- 142
LF ++GK+ ++F+ + R G F FVRY ++ K R + TV+ ++
Sbjct: 188 LFDKYGKVVDIFIPKDRRTGESRGFAFVRYKYADEASKAVDRLDGRMVDGREITVQFAKY 367
Query: 143 --AKERLHLSGGFASSSRS 159
ER+H S R+
Sbjct: 368 GPNAERIHKGRIIESEERA 424
>TC12416 similar to GB|AAH56066.1|33417132|BC056066 sop-prov protein
{Xenopus laevis;} , partial (32%)
Length = 416
Score = 33.1 bits (74), Expect = 0.11
Identities = 18/53 (33%), Positives = 25/53 (46%)
Frame = -2
Query: 439 PPRPRPRGRPKRKKEVKRGLEGSRRPQGHPRKSGTMDLDAAPSPSHPSPPPQL 491
P RPRPR RP+ + + RP PR + + + A SP P P P +
Sbjct: 217 PRRPRPRPRPRPRPSPRP------RPSPRPRPNPSPNPSALGSPDAPRPSPDI 77
Score = 27.7 bits (60), Expect = 4.8
Identities = 18/55 (32%), Positives = 22/55 (39%)
Frame = -2
Query: 440 PRPRPRGRPKRKKEVKRGLEGSRRPQGHPRKSGTMDLDAAPSPSHPSPPPQLHPA 494
PRPRPR RP RP PR S + +P+PS P P+
Sbjct: 208 PRPRPRPRP--------------RPSPRPRPSPRPRPNPSPNPSALGSPDAPRPS 86
>TC8576 similar to UP|Q9SJN3 (Q9SJN3) At2g36220 protein
(At2g36220/F2H17.17), partial (17%)
Length = 604
Score = 32.7 bits (73), Expect = 0.15
Identities = 21/58 (36%), Positives = 28/58 (48%), Gaps = 4/58 (6%)
Frame = +2
Query: 438 SPPRPRPRGRPKRK--KEVKRGLEGSRRPQGHPRKSGTMDLDAA--PSPSHPSPPPQL 491
SP RP P R R + ++R GSRR + + T A P +HP+PPP L
Sbjct: 224 SPARP*PTRRIHRSATRILRRSHSGSRRMKNTTGSTATPSTSAKNPPKEAHPAPPPTL 397
>AI967582
Length = 356
Score = 32.0 bits (71), Expect = 0.26
Identities = 14/32 (43%), Positives = 19/32 (58%)
Frame = +2
Query: 94 VFLQRKRNRGRKFRFGFVRYANREDVMKGRSR 125
VF+QRK GR+F FGFV + + D +R
Sbjct: 20 VFVQRKHEVGRRFHFGFVHFLSWSDAXTAVAR 115
>TC16529 similar to UP|Q9M549 (Q9M549) Poly(A)-binding protein, partial
(22%)
Length = 761
Score = 31.6 bits (70), Expect = 0.33
Identities = 20/68 (29%), Positives = 33/68 (48%), Gaps = 3/68 (4%)
Frame = +1
Query: 57 VAPPARTCF---PLFVDGIADSVNYFHVRDLFLQHGKLTNVFLQRKRNRGRKFRFGFVRY 113
VAP F L+V + +VN + DLF Q G++ +V + R R +G+V +
Sbjct: 298 VAPAPNANFVTTSLYVGDLDQNVNDSQLYDLFNQVGQVVSVRVCRDLTTRRSLGYGYVNF 477
Query: 114 ANREDVMK 121
+N D +
Sbjct: 478 SNPPDAAR 501
>BI418438
Length = 431
Score = 31.6 bits (70), Expect = 0.33
Identities = 17/55 (30%), Positives = 21/55 (37%)
Frame = +1
Query: 439 PPRPRPRGRPKRKKEVKRGLEGSRRPQGHPRKSGTMDLDAAPSPSHPSPPPQLHP 493
PP P P R K K + S P T L ++ +P P PPP P
Sbjct: 250 PPSPTPPPRQKSSKPARPPASPSNANPPSPTSLLTQPLSSSSNPPSPPPPPTSPP 414
>TC13226 similar to UP|HMGB_SOYBN (Q10370) HMG-Y related protein B (SB16B
protein) (Fragment), partial (60%)
Length = 474
Score = 30.8 bits (68), Expect = 0.57
Identities = 20/66 (30%), Positives = 29/66 (43%), Gaps = 4/66 (6%)
Frame = +3
Query: 435 LDFSPPRPRPRGRPKRKKEVKRGLE----GSRRPQGHPRKSGTMDLDAAPSPSHPSPPPQ 490
LD PRPRGRP + + + S RP+G P+K A+P + S P+
Sbjct: 66 LDTVVAPPRPRGRPPKDPNAEPKPKVVSIASGRPRGRPKKVARSAAAASPGAAVSSGRPR 245
Query: 491 LHPAHI 496
P +
Sbjct: 246 GRPPKV 263
>TC14530 similar to UP|P82277 (P82277) Plastid-specific 30S ribosomal
protein 2, chloroplast precursor (PSRP-2), partial (68%)
Length = 1104
Score = 30.8 bits (68), Expect = 0.57
Identities = 14/55 (25%), Positives = 30/55 (54%)
Frame = +2
Query: 65 FPLFVDGIADSVNYFHVRDLFLQHGKLTNVFLQRKRNRGRKFRFGFVRYANREDV 119
+ ++V +A +V +++ F + GK+ + + R + FGFV +++ EDV
Sbjct: 530 YKVYVGNLAKTVTSDMLKNFFSEKGKVLSAKVSRVPGTSKSSGFGFVTFSSDEDV 694
>BP081080
Length = 458
Score = 30.8 bits (68), Expect = 0.57
Identities = 13/18 (72%), Positives = 14/18 (77%)
Frame = +1
Query: 65 FPLFVDGIADSVNYFHVR 82
F LFVDGI+D VNY VR
Sbjct: 403 FTLFVDGISDGVNYLQVR 456
>AV773507
Length = 496
Score = 30.8 bits (68), Expect = 0.57
Identities = 23/94 (24%), Positives = 42/94 (44%), Gaps = 6/94 (6%)
Frame = +2
Query: 71 GIADSVNYFHVRDLFLQHGKLTNVFLQRKRNRGRKFRFGFVRYANR---EDVMK---GRS 124
G++ V + F + GK+ + +R+ GR FGF+ +A+R ED +K GR
Sbjct: 29 GLSWDVTERQLEHAFDRDGKILECQIMMERDTGRPRGFGFITFADRRGMEDAIKEMHGRE 208
Query: 125 RKQSYVSMAPPTTVKDSRAKERLHLSGGFASSSR 158
+S+ ++ + GG++S R
Sbjct: 209 IGDRIISVNKAQPKMGGDDADQGYRGGGYSSGGR 310
>TC11683 similar to UP|Q94AC3 (Q94AC3) AT4g36960/C7A10_400, partial (38%)
Length = 664
Score = 30.0 bits (66), Expect = 0.97
Identities = 15/60 (25%), Positives = 29/60 (48%)
Frame = +1
Query: 60 PARTCFPLFVDGIADSVNYFHVRDLFLQHGKLTNVFLQRKRNRGRKFRFGFVRYANREDV 119
P + +F+ I SV R F ++G +T++++ + + GF+ YAN + V
Sbjct: 484 PVKKVNRIFIARIPPSVTEATFRSHFEKYGDITDLYMPKDQRSKTHRGIGFITYANADSV 663
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.320 0.138 0.424
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,281,428
Number of Sequences: 28460
Number of extensions: 165714
Number of successful extensions: 1979
Number of sequences better than 10.0: 101
Number of HSP's better than 10.0 without gapping: 1757
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1920
length of query: 526
length of database: 4,897,600
effective HSP length: 94
effective length of query: 432
effective length of database: 2,222,360
effective search space: 960059520
effective search space used: 960059520
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 57 (26.6 bits)
Lotus: description of TM0005.12