Lotus
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= TM0004a.4
         (791 letters)

Database: LJGI 
           28,460 sequences; 14,692,800 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

AV778315                                                              138  3e-33
BP033560                                                               39  0.003
AV772472                                                               30  1.9
NP459503 maturase for trnK intron [Lotus japonicus]                    29  3.3
BP058026                                                               27  9.6

>AV778315 
          Length = 559

 Score =  138 bits (348), Expect = 3e-33
 Identities = 62/65 (95%), Positives = 64/65 (98%)
 Frame = -3

Query: 678 SDRLQWHGHFPGKPDWSETSRFVAFTMVDSVKREIYIAFNTSHLPVTITLPERPGYRWEP 737
           SDRLQWHGHFPGKPDWSETSRFVAFTM+DSVKREIYIAFNTSHLPVTITLPER GYRWEP
Sbjct: 557 SDRLQWHGHFPGKPDWSETSRFVAFTMLDSVKREIYIAFNTSHLPVTITLPERAGYRWEP 378

Query: 738 LVDTG 742
           L+DTG
Sbjct: 377 LLDTG 363



 Score = 95.1 bits (235), Expect = 4e-20
 Identities = 50/70 (71%), Positives = 51/70 (72%)
 Frame = -1

Query: 719 SHLPVTITLPERPGYRWEPLVDTGKSAPYDFLTPDLPGRDIAIQQYAQFLDANLYPMLSY 778
           S LP    L       W P     +SAPYDFLTPDLPGRDIAI QYAQFLDANLYPMLSY
Sbjct: 421 SRLPCPSVLDTDGNPSWTP----AQSAPYDFLTPDLPGRDIAIPQYAQFLDANLYPMLSY 254

Query: 779 SSIILLRTPD 788
           SSIILLRTPD
Sbjct: 253 SSIILLRTPD 224


>BP033560 
          Length = 527

 Score = 38.9 bits (89), Expect = 0.003
 Identities = 16/36 (44%), Positives = 24/36 (66%)
 Frame = +1

Query: 74  FQVSRGYPAPFGATVRDGGVNFAIYSLNAVSATLCL 109
           +++S G   P G +  D G+NFAI+S +A + TLCL
Sbjct: 391 WEISPGQAVPLGVSQVDNGINFAIFSQHATTVTLCL 498


>AV772472 
          Length = 423

 Score = 29.6 bits (65), Expect = 1.9
 Identities = 19/56 (33%), Positives = 29/56 (50%), Gaps = 4/56 (7%)
 Frame = +1

Query: 32  SKRVSEKHKSICSTTKILATGNG----SGFETETTLVVDKPQLGGRFQVSRGYPAP 83
           S  V  + KS  +TT  +ATG G    +GF T ++  + +P L G   +S   P+P
Sbjct: 244 SVHVVTRMKSAATTT--IATGRGPHTSAGFHTHSSRALHRPDLQGHRAISSPTPSP 405


>NP459503 maturase for trnK intron [Lotus japonicus]
          Length = 1527

 Score = 28.9 bits (63), Expect = 3.3
 Identities = 26/108 (24%), Positives = 49/108 (45%), Gaps = 4/108 (3%)
 Frame = +1

Query: 336 GIEVIMDVVFNHTVEGN-ENGPII-SFRGVDN--SIYYMIAPKGEFYNYSGCGNTLNCNH 391
           G  +I++++F+  +  + E   II S++ + +  SI+     K  + NY    + +   +
Sbjct: 289 GFAIIVEILFSLQLSSSLEEAEIIKSYKNLRSIHSIFPFFEDKVTYLNYI---SDIRVPY 459

Query: 392 PVVRQFIVDCLRYWVTEMHVDGFRFDLASIMTRSSSLWNGVNVFGTSI 439
           P+  + +V  LRYWV     D   F L  +       WN + +   SI
Sbjct: 460 PIHLEILVQILRYWVK----DAPLFHLLRLFLYDYCNWNSIFIPQKSI 591


>BP058026 
          Length = 389

 Score = 27.3 bits (59), Expect = 9.6
 Identities = 10/27 (37%), Positives = 17/27 (62%)
 Frame = +3

Query: 553 LVTYNNKHNLPNGEDNNDGENHNNSWN 579
           ++  NN +N  N  +NN+  N+NN+ N
Sbjct: 3   IINNNNNNNNNNNNNNNNNNNNNNNNN 83


  Database: LJGI
    Posted date:  Jul 30, 2004 11:16 AM
  Number of letters in database: 14,692,800
  Number of sequences in database:  28,460
  
Lambda     K      H
   0.320    0.139    0.443 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,689,787
Number of Sequences: 28460
Number of extensions: 244731
Number of successful extensions: 1131
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1101
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1113
length of query: 791
length of database: 4,897,600
effective HSP length: 98
effective length of query: 693
effective length of database: 2,108,520
effective search space: 1461204360
effective search space used: 1461204360
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 59 (27.3 bits)


Lotus: description of TM0004a.4