BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0490.17
(392 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
NP501836 dehiscence-related endopolygalaturonase [Glycine max] 230 9e-61
NP004759 polygalacturonase PG1 225 2e-59
TC221920 UP|Q9SWS2 (Q9SWS2) Polygalacturonase PG2, complete 221 3e-58
TC231585 weakly similar to UP|Q9M7D3 (Q9M7D3) Polygalacturonase,... 177 7e-45
TC212447 weakly similar to UP|O82586 (O82586) Endopolygalacturon... 126 2e-43
CA853310 122 2e-28
TC231081 weakly similar to UP|Q9M7D3 (Q9M7D3) Polygalacturonase,... 96 1e-24
BE346184 99 3e-21
BG650346 similar to GP|6624205|dbj polygalacturonase {Cucumis sa... 88 6e-18
CA801781 86 2e-17
TC222699 weakly similar to UP|Q9LW07 (Q9LW07) Polygalacturonase-... 86 4e-17
TC208008 homologue to UP|Q94KI6 (Q94KI6) Polygalacturonase (Frag... 77 1e-14
TC217917 similar to UP|Q9M1L0 (Q9M1L0) Polygalacturonase-like pr... 73 2e-13
TC223820 weakly similar to UP|Q9M1Y9 (Q9M1Y9) Polygalacturonase-... 65 4e-11
TC218204 similar to UP|Q9FJ27 (Q9FJ27) Polygalacturonase-like pr... 64 1e-10
TC217817 similar to UP|Q84LI7 (Q84LI7) Polygalacturonase-like pr... 55 4e-08
TC205977 similar to UP|Q84LI7 (Q84LI7) Polygalacturonase-like pr... 55 5e-08
TC218721 similar to UP|Q9FJ27 (Q9FJ27) Polygalacturonase-like pr... 50 2e-07
TC206561 52 5e-07
TC209841 50 2e-06
>NP501836 dehiscence-related endopolygalaturonase [Glycine max]
Length = 1287
Score = 230 bits (586), Expect = 9e-61
Identities = 130/356 (36%), Positives = 193/356 (53%), Gaps = 9/356 (2%)
Frame = +1
Query: 34 GAPN---SDITQAFLNAWKQACASTSPSKIVISKATYKMNAVNVKGPCKAPIEIQVDGTI 90
GAP +D TQAF AW+ AC+S + V+ + Y + GPC++ IE+Q+ G I
Sbjct: 202 GAPGDGKTDDTQAFKEAWEVACSSGG-AVFVVPRKNYLLKPFTFSGPCESDIEVQISGII 378
Query: 91 QAPTDPNALNGAF-QWVTIGYVDYFTLSGNGTFDGRGASAWKLQKACGAT----CSKRSM 145
+A + + + W+ ++ ++ G GT DG G W Q +C C
Sbjct: 379 EASENLSDYSEDLTHWLVFDSIEKLSVKGGGTIDGNGNIWW--QNSCKVNEKLPCKNAPT 552
Query: 146 NIGFNFLKHSTVRSVTSKDSKFFHVTLLGCTNFTFDGFKIIAPGDSISINTDGIHIAKST 205
+ F K TV +T K+ + V+ N + APGDS NTDGIH+ +
Sbjct: 553 ALTFYKCKDLTVEDLTIKNGQQMQVSFQNSENVQVSDLTVTAPGDSP--NTDGIHVTNTQ 726
Query: 206 DVKILNSNIATGDDCVSLGDGNKHITIHNVKCGPGHGFSIGSLGTNPTEEPVENIIVKNC 265
+++I NS I TGDDC+S+ G+K + ++ CGPGHG SIGSLG +++ V I VK
Sbjct: 727 NIQISNSVIGTGDDCISIVSGSKDVLATDIICGPGHGISIGSLGAEGSKDFVSGITVKGA 906
Query: 266 TLTNTQNGVRIKTWPTAPGTSPVTNLTFEDITMVNVYNPVIIDQQYCPSNLCSKQSPSKI 325
L+ T NG+RIKTW G++ +N+ F++I M NV NP+IIDQ YC ++ S +
Sbjct: 907 QLSGTTNGLRIKTWQGGSGSA--SNIQFQNIQMDNVANPIIIDQNYCDQETPCEEQTSAV 1080
Query: 326 KISNVTFKNIKGTSETKDGVILNCSSGVPCEGVELSDISLTF-NGAPAMAKCANVK 380
+I NV ++NI GTS + GV +CS PC+G+ L +I L G A A C +V+
Sbjct: 1081QIRNVLYQNISGTSASDVGVQFDCSKKFPCQGIVLQNIDLKLEGGGEAKASCNSVE 1248
>NP004759 polygalacturonase PG1
Length = 1332
Score = 225 bits (574), Expect = 2e-59
Identities = 130/363 (35%), Positives = 194/363 (52%), Gaps = 15/363 (4%)
Frame = +1
Query: 18 LSGSAQSIVFDIRTFG--GAPNSDITQAFLNAWKQACASTSPSKIVISKATYKMNAVNVK 75
L +A + F++ +G G ++D T+AF +AW AC + +V S + + + ++
Sbjct: 109 LKAAASATSFNVLDYGAKGDGHADDTKAFEDAWAAACKVEGSTMVVPSGSVFLVKPISFS 288
Query: 76 GP-CKAPIEIQVDGTIQAPTDPNAL-NGAFQWVTIGYVDYFTLSGNGTFDGRGASAWK-- 131
GP C+ I Q+DG I APT A +G QW+ ++ T+ G G DG+G+ W
Sbjct: 289 GPNCEPNIVFQLDGKIIAPTSSEAWGSGTLQWLEFSKLNTITIRGKGVIDGQGSVWWNND 468
Query: 132 ---------LQKACGATCSKRSMNIGFNFLKHSTVRSVTSKDSKFFHVTLLGCTNFTFDG 182
+ ++ G S + + F TV +T ++S+ H+ CTN G
Sbjct: 469 SPTYNPTEVMLESNGRLPSTKPTALRFYGSDGVTVTGITIQNSQQTHLKFDSCTNVQVSG 648
Query: 183 FKIIAPGDSISINTDGIHIAKSTDVKILNSNIATGDDCVSLGDGNKHITIHNVKCGPGHG 242
+ +PGDS NTDGIH+ S +V I +S +A GDDCVS+ G I +HNV CGPGHG
Sbjct: 649 ISVSSPGDSP--NTDGIHLQNSQNVVIYSSTLACGDDCVSIQTGCSDIYVHNVNCGPGHG 822
Query: 243 FSIGSLGTNPTEEPVENIIVKNCTLTNTQNGVRIKTWPTAPGTSPVTNLTFEDITMVNVY 302
SIGSLG T+ V N+ V++ T+ NT GVRIKTW G+ V N+ F ++ + V
Sbjct: 823 ISIGSLGRENTKACVRNVTVRDVTIQNTLTGVRIKTW--QGGSGSVQNIMFSNVQVSGVQ 996
Query: 303 NPVIIDQQYCPSNLCSKQSPSKIKISNVTFKNIKGTSETKDGVILNCSSGVPCEGVELSD 362
P+ IDQ YC C +S S + +S + + N+KGT TK+ + CS +PC G+ L
Sbjct: 997 TPISIDQYYCDGGRCRNES-SAVAVSGIHYVNVKGT-YTKEPIYFACSDNLPCSGITLDT 1170
Query: 363 ISL 365
I L
Sbjct: 1171IQL 1179
>TC221920 UP|Q9SWS2 (Q9SWS2) Polygalacturonase PG2, complete
Length = 1660
Score = 221 bits (564), Expect = 3e-58
Identities = 129/360 (35%), Positives = 192/360 (52%), Gaps = 15/360 (4%)
Frame = +1
Query: 21 SAQSIVFDIRTFGGAP--NSDITQAFLNAWKQACASTSPSKIVISKATYKMNAVNVKGP- 77
+A + F++ +G ++D T+AF NAW AC + +V S + + + ++ GP
Sbjct: 190 AAYATTFNVLDYGAKDGGHADDTKAFQNAWVAACKVEGSTMVVPSGSVFLVKPISFSGPN 369
Query: 78 CKAPIEIQVDGTIQAPTDPNAL-NGAFQWVTIGYVDYFTLSGNGTFDGRGASAWK----- 131
C+ I Q+DG I APT A +G QW+ ++ T+ G G DG+G+ W
Sbjct: 370 CEPNIVFQLDGKIIAPTGSAAWGSGTLQWLEFSKLNKITIRGKGVIDGQGSVWWNNDSPT 549
Query: 132 ------LQKACGATCSKRSMNIGFNFLKHSTVRSVTSKDSKFFHVTLLGCTNFTFDGFKI 185
+ ++ G S + + F TV +T ++S+ H+ CTN G +
Sbjct: 550 YNPTEVMLESNGRLPSTKPTALRFYGSDGVTVTGITIQNSQQTHLKFDSCTNVQVSGISV 729
Query: 186 IAPGDSISINTDGIHIAKSTDVKILNSNIATGDDCVSLGDGNKHITIHNVKCGPGHGFSI 245
+PGDS NTDGIH+ S +V I +S +A GDDC+S+ G I +HNV CGPGHG SI
Sbjct: 730 SSPGDSP--NTDGIHLQNSQNVVIYSSTLACGDDCISIQTGCSDIYVHNVNCGPGHGISI 903
Query: 246 GSLGTNPTEEPVENIIVKNCTLTNTQNGVRIKTWPTAPGTSPVTNLTFEDITMVNVYNPV 305
GSLG T+ V N+ V++ T+ NT GVRIKTW G+ V N+ F ++ + V P+
Sbjct: 904 GSLGRENTKACVRNVTVRDVTIQNTLTGVRIKTW--QGGSGSVQNIMFSNVQVSGVQIPI 1077
Query: 306 IIDQQYCPSNLCSKQSPSKIKISNVTFKNIKGTSETKDGVILNCSSGVPCEGVELSDISL 365
+IDQ YC C +S S + +S + + NIKGT TK + CS +PC G+ L I L
Sbjct: 1078LIDQYYCDGGKCRNES-SAVAVSAIHYVNIKGT-YTKQPIYFACSDNLPCTGITLDTIRL 1251
>TC231585 weakly similar to UP|Q9M7D3 (Q9M7D3) Polygalacturonase, partial
(48%)
Length = 878
Score = 177 bits (449), Expect = 7e-45
Identities = 89/213 (41%), Positives = 130/213 (60%)
Frame = +2
Query: 153 KHSTVRSVTSKDSKFFHVTLLGCTNFTFDGFKIIAPGDSISINTDGIHIAKSTDVKILNS 212
K VR++ +S+ H++ C K++AP + S NTDGIHI+ + V++ +S
Sbjct: 8 KXXKVRNLMLINSQRMHLSFTNCMRIVASHLKVLAP--AFSPNTDGIHISATKGVEVRDS 181
Query: 213 NIATGDDCVSLGDGNKHITIHNVKCGPGHGFSIGSLGTNPTEEPVENIIVKNCTLTNTQN 272
I TGDDC+S+ + + I N+ CGPGHG SIGSLG + E V+N+IV L NT N
Sbjct: 182 VIRTGDDCISIVRNSSRVWIRNISCGPGHGISIGSLGKSKKWEKVQNVIVDGVYLYNTDN 361
Query: 273 GVRIKTWPTAPGTSPVTNLTFEDITMVNVYNPVIIDQQYCPSNLCSKQSPSKIKISNVTF 332
GVRIKTW G + + +TF+ I M NV NP+I+DQ YC S K S +++ N++F
Sbjct: 362 GVRIKTWQGGSGFA--SKITFQHILMENVSNPIIVDQYYCDSRNPCKNETSAVRVENISF 535
Query: 333 KNIKGTSETKDGVILNCSSGVPCEGVELSDISL 365
+I+GTS T++ + +CS PCEG+ L +I L
Sbjct: 536 IDIQGTSATEEAIKFSCSDAFPCEGLYLENIFL 634
>TC212447 weakly similar to UP|O82586 (O82586) Endopolygalacturonase, partial
(54%)
Length = 852
Score = 126 bits (317), Expect(2) = 2e-43
Identities = 69/143 (48%), Positives = 93/143 (64%), Gaps = 3/143 (2%)
Frame = +3
Query: 232 IHNVKCGPGHGFSIGSLGTNPTEEPVENIIVKNCTLTNTQNGVRIKTWPTAPGTSPVTNL 291
+ +V CGPGHG SIGSLG + E V+N+ V+ T + TQNG RIK+W P + V ++
Sbjct: 231 VGDVACGPGHGISIGSLGWDLDEPGVKNVTVRKATFSKTQNGFRIKSWGR-PSSGFVEDV 407
Query: 292 TFEDITMVNVYNPVIIDQQYCP-SNLCSKQSPSKIKISNVTFKNIKGTSETKDGVILNCS 350
FE TM +V NP+IIDQ YCP N C Q+ S +KIS++++K+I GTS T+ V +CS
Sbjct: 408 HFEHATMSDVQNPIIIDQHYCPFRNGCPSQA-SGVKISDISYKDIHGTSATQVAVKFDCS 584
Query: 351 SGVPCEGVELSDISLTF--NGAP 371
S PCE + L DI T+ N AP
Sbjct: 585 SEQPCERITLEDIRFTYKINKAP 653
Score = 67.8 bits (164), Expect(2) = 2e-43
Identities = 33/77 (42%), Positives = 48/77 (61%)
Frame = +2
Query: 154 HSTVRSVTSKDSKFFHVTLLGCTNFTFDGFKIIAPGDSISINTDGIHIAKSTDVKILNSN 213
H + +TS +S+ H+ + C N G K++A G+S NTDGIH+ STDV IL
Sbjct: 2 HIVITGLTSLNSQLVHILINACHNVKMHGVKLMADGNSP--NTDGIHVKFSTDVTILAPR 175
Query: 214 IATGDDCVSLGDGNKHI 230
I TGDDC+S+G G +++
Sbjct: 176 IRTGDDCISVGPGCRNL 226
>CA853310
Length = 555
Score = 122 bits (307), Expect = 2e-28
Identities = 71/157 (45%), Positives = 92/157 (58%), Gaps = 6/157 (3%)
Frame = +1
Query: 127 ASAWKLQKAC------GATCSKRSMNIGFNFLKHSTVRSVTSKDSKFFHVTLLGCTNFTF 180
ASAW Q+ C GATC + + FN ++ TS + HVTL C
Sbjct: 79 ASAW-WQQPCVGNPQPGATCRPPTA-VTFNRCNRLQLKGYTSINPARSHVTLTSCNKGII 252
Query: 181 DGFKIIAPGDSISINTDGIHIAKSTDVKILNSNIATGDDCVSLGDGNKHITIHNVKCGPG 240
++IAPG S NTDGI I+ ST +++LNS IATGDDC+++ G+ I I + CGPG
Sbjct: 253 SNIRLIAPGTSP--NTDGIDISGSTGIQVLNSFIATGDDCIAISAGSSKIKITGITCGPG 426
Query: 241 HGFSIGSLGTNPTEEPVENIIVKNCTLTNTQNGVRIK 277
HG SIGSLGT + VE++ V+NCTLT T GVR K
Sbjct: 427 HGISIGSLGTRGDTDIVEDVHVENCTLTETLTGVRTK 537
Score = 29.6 bits (65), Expect = 2.5
Identities = 22/67 (32%), Positives = 30/67 (43%), Gaps = 1/67 (1%)
Frame = +1
Query: 266 TLTNTQNGVRIKTWPTAPGTSPVTN-LTFEDITMVNVYNPVIIDQQYCPSNLCSKQSPSK 324
TLT+ G+ APGTSP T+ + T + V N I C + SK
Sbjct: 223 TLTSCNKGIISNIRLIAPGTSPNTDGIDISGSTGIQVLNSFIATGDDC---IAISAGSSK 393
Query: 325 IKISNVT 331
IKI+ +T
Sbjct: 394 IKITGIT 414
>TC231081 weakly similar to UP|Q9M7D3 (Q9M7D3) Polygalacturonase, partial
(25%)
Length = 690
Score = 95.5 bits (236), Expect(2) = 1e-24
Identities = 53/165 (32%), Positives = 83/165 (50%), Gaps = 4/165 (2%)
Frame = +1
Query: 85 QVDGTIQAPTDPNALNGAFQ--WVTIGYVDYFTLSGNGTFDGRGASAWKLQKACGAT--C 140
++ GTI AP DP +G Q W+ V++ T+ G G +G G W +T C
Sbjct: 202 EISGTIVAPQDPVVWHGLNQRKWLYFHGVNHLTVDGGGRINGMGQEWWARSCKINSTNPC 381
Query: 141 SKRSMNIGFNFLKHSTVRSVTSKDSKFFHVTLLGCTNFTFDGFKIIAPGDSISINTDGIH 200
+ F+ + VR + DS+ H++ C ++AP + S NTDGIH
Sbjct: 382 HPAPTAMTFHRXQDLXVRHLMLIDSQRMHLSFTNCMRIGAXHLXVLAP--AFSPNTDGIH 555
Query: 201 IAKSTDVKILNSNIATGDDCVSLGDGNKHITIHNVKCGPGHGFSI 245
I+ + V++++S I TGDDC+ + + I N+ CGPGHG S+
Sbjct: 556 ISVTXGVEVMHSXIRTGDDCIXHSRKSSRVWIRNISCGPGHGISM 690
Score = 35.4 bits (80), Expect(2) = 1e-24
Identities = 16/46 (34%), Positives = 29/46 (62%), Gaps = 1/46 (2%)
Frame = +2
Query: 41 TQAFLNAWKQACASTSPSKIVISKA-TYKMNAVNVKGPCKAPIEIQ 85
T+AFL AWK AC+ + +V T+ ++ V++ GPC++ I ++
Sbjct: 41 TEAFLEAWKIACSLSGFISVVFPYGKTFLVHPVDIGGPCRSKITLR 178
>BE346184
Length = 458
Score = 99.4 bits (246), Expect = 3e-21
Identities = 56/152 (36%), Positives = 78/152 (50%), Gaps = 9/152 (5%)
Frame = +3
Query: 112 DYFTLSGNGTFDGRGASAWKLQ---------KACGATCSKRSMNIGFNFLKHSTVRSVTS 162
D TL+G GT +G G W L K C +M I F + V+ +
Sbjct: 3 DQMTLNGTGTIEGNGDKWWDLPCKPHRGPNGKTLSGPCGSPAM-IRFFMSSNLKVKGLKI 179
Query: 163 KDSKFFHVTLLGCTNFTFDGFKIIAPGDSISINTDGIHIAKSTDVKILNSNIATGDDCVS 222
++S FH+ GC D I +P +S NTDGIH+ S V I NS I+ GDDC+S
Sbjct: 180 QNSPQFHMIFNGCQGVLIDKLSISSP--KLSPNTDGIHVENSKYVGIYNSMISNGDDCIS 353
Query: 223 LGDGNKHITIHNVKCGPGHGFSIGSLGTNPTE 254
+G G+ ++ I + CGP HG SIGSLG + ++
Sbjct: 354 IGPGSSNVDIAGLTCGPSHGISIGSLGVHNSQ 449
>BG650346 similar to GP|6624205|dbj polygalacturonase {Cucumis sativus},
partial (31%)
Length = 414
Score = 88.2 bits (217), Expect = 6e-18
Identities = 42/81 (51%), Positives = 56/81 (68%)
Frame = +3
Query: 169 HVTLLGCTNFTFDGFKIIAPGDSISINTDGIHIAKSTDVKILNSNIATGDDCVSLGDGNK 228
H T+ C + G K+ APGDS NTDGIHI++ST+V I +S I TGDDC+S+ + +
Sbjct: 144 HFTISRCDSVRITGVKVSAPGDSP--NTDGIHISESTNVIIQDSKIGTGDDCISIVNASS 317
Query: 229 HITIHNVKCGPGHGFSIGSLG 249
+I + + CGPGHG SIGSLG
Sbjct: 318 YIKMKRIYCGPGHGISIGSLG 380
>CA801781
Length = 445
Score = 86.3 bits (212), Expect = 2e-17
Identities = 50/126 (39%), Positives = 76/126 (59%), Gaps = 2/126 (1%)
Frame = +1
Query: 247 SLGTNPTEEPVENIIVKNCTLTNTQNGVRIKTWPTAPGTSPVTNLTFEDITMVNVYNPVI 306
SLG + ++ V N+ V++ + + NG+RIKTW G+ V++L FE+I M NV N +I
Sbjct: 1 SLGVHNSQACVSNLTVRDSIIRESDNGLRIKTWQGGMGS--VSSLRFENIQMENVGNCII 174
Query: 307 IDQQYCPSNLCSKQSPSKIKISNVTFKNIKGTSETKDGVI-LNCSSGVPCEGVELSDIS- 364
IDQ YC S C Q+ S + +++V++ NIKGT + + I CS V C + LS++
Sbjct: 175 IDQYYCMSKECLNQT-SAVHLNDVSYSNIKGTYDVRTAPIHFACSDTVACTNITLSEVEL 351
Query: 365 LTFNGA 370
L F GA
Sbjct: 352 LPFEGA 369
>TC222699 weakly similar to UP|Q9LW07 (Q9LW07) Polygalacturonase-like
protein, partial (21%)
Length = 721
Score = 85.5 bits (210), Expect = 4e-17
Identities = 64/203 (31%), Positives = 102/203 (49%), Gaps = 11/203 (5%)
Frame = +1
Query: 27 FDIRTFGGAPN--SDITQAFLNAWKQAC-ASTSPSKIVISKA-TYKMNAVNVKGPCKAP- 81
F++ +G N +D +QAFL AWK AC AS + ++I K T+ + V +GPCK P
Sbjct: 127 FNVIDYGATGNGQTDDSQAFLKAWKDACNASYGTATLLIPKEKTFMLQPVLFRGPCKPPT 306
Query: 82 IEIQVDGTIQAPTDPNAL----NGAFQWVTIGYVDYFTLSGNG--TFDGRGASAWKLQKA 135
+ I++ GTI AP A + W+ ++ + G G DG+G+ W +
Sbjct: 307 VHIKLKGTIIAPNKIEAWKLPKSTRMAWIRFRHISGLVIRGGGWGLIDGQGSPWWN---S 477
Query: 136 CGATCSKRSMNIGFNFLKHSTVRSVTSKDSKFFHVTLLGCTNFTFDGFKIIAPGDSISIN 195
T KR + F + + +T +S H+++ C N +IAP +S N
Sbjct: 478 YFNTEIKRPTALHFRECDYLFLSGLTHINSPKNHISINRCNNSLISKIHMIAPDESP--N 651
Query: 196 TDGIHIAKSTDVKILNSNIATGD 218
TDGI I++S+++ I NS + TGD
Sbjct: 652 TDGIDISQSSNIVIKNSKMETGD 720
>TC208008 homologue to UP|Q94KI6 (Q94KI6) Polygalacturonase (Fragment),
partial (46%)
Length = 1144
Score = 77.0 bits (188), Expect = 1e-14
Identities = 42/117 (35%), Positives = 63/117 (52%)
Frame = +3
Query: 249 GTNPTEEPVENIIVKNCTLTNTQNGVRIKTWPTAPGTSPVTNLTFEDITMVNVYNPVIID 308
G + T V NI V++ + NT NGVRIKTW G+ V + F +I + V P++ID
Sbjct: 435 GKDNTRACVSNITVRDVNMHNTMNGVRIKTW--QGGSGSVQGVLFSNIQVSEVELPIVID 608
Query: 309 QQYCPSNLCSKQSPSKIKISNVTFKNIKGTSETKDGVILNCSSGVPCEGVELSDISL 365
Q YC C Q+ S + ++ + ++ I+GT K V CS +PC V L+ + L
Sbjct: 609 QFYCDKRTCKNQT-SAVSLAGINYERIRGTYTVKP-VHFACSDSLPCVDVSLTSVEL 773
>TC217917 similar to UP|Q9M1L0 (Q9M1L0) Polygalacturonase-like protein
(AT3g42950/F18P9_110), partial (74%)
Length = 1292
Score = 73.2 bits (178), Expect = 2e-13
Identities = 78/285 (27%), Positives = 127/285 (44%), Gaps = 18/285 (6%)
Frame = +2
Query: 119 NGTFDGRGASAWKL--QKACGATCSKRSMNIGFNFLKHSTVRSVTSKDSKFFHVTLLGCT 176
NGT +G+G + WK QK T R + F + ++T +DS F+ + C
Sbjct: 170 NGTINGQGQTWWKKYRQKRLNHT---RGPLVQIMFSSDIVITNITLRDSPFWTLHPYDCK 340
Query: 177 NFTFDGFKIIAPGDSISINTDGIHIAKSTDVKILNSNIATGDDCVSLGDG---------- 226
N T G I+AP + NTDGI D+ I + I+ GDD +++ G
Sbjct: 341 NITIKGVTILAPVFG-APNTDGIDPDSCEDMLIEDCYISVGDDAIAIKSGWDQYGIAYGR 517
Query: 227 -NKHITIHN--VKCGPGHGFSIGSLGTNPTEEPVENIIVKNCTLTNTQNGVRIKTWPTAP 283
+ +I I N V+ G SIGS V N+ V+N + +++ GVRIK TA
Sbjct: 518 PSMNIMIRNLVVRSMVSAGISIGS----EMSGGVSNVTVENILIWDSRRGVRIK---TAR 676
Query: 284 GTSP-VTNLTFEDITMVNVYNPVIIDQQYCPSNLCSKQSPSKIKISNVTFKNIKGTSETK 342
G V +T+ +IT NV +++ Y + + +++F + G +
Sbjct: 677 GRGAYVRQITYRNITFENVRVGIVMKTDYNEHPDDGYDPMALPILRDISFTTVHGQG-VR 853
Query: 343 DGVILNCSSGVPCEGVELSDIS--LTFNGAPAMAKCANVKPIIIG 385
V ++ S +P V D+S LT+ + +CA V+ +IG
Sbjct: 854 VPVRIHGSEEIPVRNVTFQDMSVGLTYK-KKHIFQCAFVQGRVIG 985
>TC223820 weakly similar to UP|Q9M1Y9 (Q9M1Y9) Polygalacturonase-like
protein, partial (13%)
Length = 476
Score = 65.5 bits (158), Expect = 4e-11
Identities = 40/142 (28%), Positives = 69/142 (48%), Gaps = 4/142 (2%)
Frame = +2
Query: 11 IVLFLLLLSGSAQSIVFDIRTFGGAPN--SDITQAFLNAWKQACASTSPSKIVISKATYK 68
+VL + SA++ +++ FG P+ +D T AFL+AW +AC+S P+ I + + +
Sbjct: 53 LVLCTWFSTSSAKATSYNVVDFGAKPDGATDATAAFLSAWNKACSSNKPAGIHVPQGRFL 232
Query: 69 M-NAVNVKGPC-KAPIEIQVDGTIQAPTDPNALNGAFQWVTIGYVDYFTLSGNGTFDGRG 126
+ AV G C I I + GT+ AP+ + + W+T V ++ G G D RG
Sbjct: 233 IARAVTFHGQCANRAISITIRGTLVAPSQYTFVGNSLYWLTFDQVSGVSIHG-GVLDARG 409
Query: 127 ASAWKLQKACGATCSKRSMNIG 148
+ W + C + +G
Sbjct: 410 SFLWDCKYKATPNCPIGAATLG 475
>TC218204 similar to UP|Q9FJ27 (Q9FJ27) Polygalacturonase-like protein,
partial (51%)
Length = 1014
Score = 63.9 bits (154), Expect = 1e-10
Identities = 70/245 (28%), Positives = 110/245 (44%), Gaps = 22/245 (8%)
Frame = +2
Query: 157 VRSVTSKDSKFFHVTLLGCTNFTFDGFKIIAPGDSISINTDGIHIAKSTDVKILNSNIAT 216
+ +V K+S F+++ + C+N I+AP DS NTDGI S++V I +S I+T
Sbjct: 56 ISNVIFKNSPFWNIHPVYCSNVVVRYVTILAPRDSP--NTDGIDPDSSSNVCIEDSYIST 229
Query: 217 GDDCVSLGDG-----------NKHITIHNVK-CGPGHGFSIGSLGTNPTEEPVENIIVKN 264
GDD V++ G + ITI V P G +IGS T VEN++ ++
Sbjct: 230 GDDLVAVKSGWDEYGIAYGRPSYGITIRRVTGSSPFAGIAIGS----ETSGGVENVLAEH 397
Query: 265 CTLTNTQNGVRIKTWPTAPGTSPVTNLTFEDITMVNVYNPVII--------DQQYCPSNL 316
L N G+ IKT G + N+T + + N + I D+++ P+ L
Sbjct: 398 INLFNMGVGIHIKTNSGRGGL--IKNITVAHVYVENARQGIKIAGDVGGHPDEKFNPNAL 571
Query: 317 CSKQSPSKIKISNVTFKNIKGTSETKDGVILNCSSGVPCEGVELSDISLTFNGAPAMA-- 374
+ +T KN+ G + G+I + P V LSDI+ P
Sbjct: 572 --------PVVKGITIKNVWGVKVNQAGLIHGLRNS-PFTDVCLSDINFHGMEGPRSPSW 724
Query: 375 KCANV 379
KC++V
Sbjct: 725 KCSDV 739
>TC217817 similar to UP|Q84LI7 (Q84LI7) Polygalacturonase-like protein,
partial (92%)
Length = 1694
Score = 55.5 bits (132), Expect = 4e-08
Identities = 51/180 (28%), Positives = 84/180 (46%), Gaps = 13/180 (7%)
Frame = +1
Query: 112 DYFTLSGNGTFDGRGASAWKL--QKACGATCSKRSMNIGFNFLKHSTVRSVTSKDSKFFH 169
D NGT DG+G W+ +K T R I F + + ++T +S ++
Sbjct: 517 DVIVTGENGTIDGQGEFWWQQFHRKKLKYT---RPYLIE*MFSDNIQISNLTLLNSPSWN 687
Query: 170 VTLLGCTNFTFDGFKIIAPGDSISINTDGIHIAKSTDVKILNSNIATGDDCVSLGDG--- 226
V + +N G I AP S NTDGI+ T+V+I + I +GDDCV++ G
Sbjct: 688 VHPVYSSNIIVQGITIYAP--VTSPNTDGINPDSCTNVRIEDCYIVSGDDCVAVKSGWDE 861
Query: 227 --------NKHITIHNVKCGPGHGFSIGSLGTNPTEEPVENIIVKNCTLTNTQNGVRIKT 278
K + I + C + +I +LG+ ++++ ++ T T++GVRIKT
Sbjct: 862 YGIKFGWPTKQLVIRRLTCISPYSATI-ALGSE-MSGGIQDVRAEDITAIQTESGVRIKT 1035
>TC205977 similar to UP|Q84LI7 (Q84LI7) Polygalacturonase-like protein,
partial (88%)
Length = 1966
Score = 55.1 bits (131), Expect = 5e-08
Identities = 64/267 (23%), Positives = 118/267 (43%), Gaps = 13/267 (4%)
Frame = +3
Query: 112 DYFTLSGNGTFDGRGASAW-KLQKACGATCSKRSMNIGFNFLKHSTVRSVTSKDSKFFHV 170
D NGT DG+G W K K G R I + + + ++T +S ++V
Sbjct: 705 DVIITGDNGTIDGQGDLWWQKFHK--GELKYTRPYLIEIMYSDNVQISNLTLVNSPSWNV 878
Query: 171 TLLGCTNFTFDGFKIIAPGDSISINTDGIHIAKSTDVKILNSNIATGDDCVSLGDG---- 226
+ +N G I+AP S NTDGI+ T+ +I + I +GDDCV++ G
Sbjct: 879 HPIYSSNLVVQGITILAP--VTSPNTDGINPDSCTNTRIEDCYIVSGDDCVAVKSGWDEY 1052
Query: 227 -------NKHITIHNVKCGPGHGFSIGSLGTNPTEEPVENIIVKNCTLTNTQNGVRIKTW 279
K + I + C ++ +LG+ ++++ ++ N+++GVRIKT
Sbjct: 1053GIAYGMPTKQLVIRRLTCISPFS-AVIALGSE-MSGGIQDVRAEDIVAINSESGVRIKTA 1226
Query: 280 PTAPGTSPVTNLTFEDITMVNVYNPVIIDQQYCPSNLCSKQSPSKIK-ISNVTFKNIKGT 338
G V ++ +TM + + Y S+ P+ + I N+ ++++
Sbjct: 1227VGRGGY--VKDIFVRRMTMKTMKWAFWMTGNY-GSHADDNYDPNALPVIQNINYRDMVAE 1397
Query: 339 SETKDGVILNCSSGVPCEGVELSDISL 365
+ T L SG P G+ +S++++
Sbjct: 1398NVTM-AARLEGISGDPFTGICISNVTI 1475
>TC218721 similar to UP|Q9FJ27 (Q9FJ27) Polygalacturonase-like protein,
partial (53%)
Length = 1128
Score = 50.4 bits (119), Expect(2) = 2e-07
Identities = 51/174 (29%), Positives = 76/174 (43%), Gaps = 12/174 (6%)
Frame = +3
Query: 185 IIAPGDSISINTDGIHIAKSTDVKILNSNIATGDDCVSLGDG-----------NKHITIH 233
I+AP DS NTDGI S++V I +S I+TGDD V++ G + ITI
Sbjct: 249 ILAPRDSP--NTDGIDPDSSSNVCIEDSYISTGDDLVAVKSGWDEYGIAYGRPSSDITIR 422
Query: 234 NVK-CGPGHGFSIGSLGTNPTEEPVENIIVKNCTLTNTQNGVRIKTWPTAPGTSPVTNLT 292
+ P G +IGS T VEN++ ++ L N G+ IKT G + N+T
Sbjct: 423 RITGSSPFAGIAIGS----ETSGGVENVLAEHINLYNMGIGIHIKTNTGRGGF--IKNIT 584
Query: 293 FEDITMVNVYNPVIIDQQYCPSNLCSKQSPSKIKISNVTFKNIKGTSETKDGVI 346
+ M + I + + + VT KN+ G + G+I
Sbjct: 585 MSHVYMEEARKGIRISGDVGDHPDDKFDANALPLVKGVTIKNVWGMKVLQAGLI 746
Score = 22.3 bits (46), Expect(2) = 2e-07
Identities = 13/61 (21%), Positives = 28/61 (45%)
Frame = +1
Query: 110 YVDYFTLSGNGTFDGRGASAWKLQKACGATCSKRSMNIGFNFLKHSTVRSVTSKDSKFFH 169
+ + + NGT DG+G W K ++ ++ + F + + +V K S F++
Sbjct: 4 HTTHIFIGENGTIDGQGDEWWNKWKQRTLQFTRPNL-VEFVNSRDIIISNVIFKSSPFWN 180
Query: 170 V 170
+
Sbjct: 181 I 183
>TC206561
Length = 1637
Score = 52.0 bits (123), Expect = 5e-07
Identities = 59/242 (24%), Positives = 99/242 (40%), Gaps = 14/242 (5%)
Frame = +1
Query: 112 DYFTLSGNGTFDGRGASAWKLQKACGATCSKRSMNIGFNFLKHSTVRSVTSKDSKFFHVT 171
D NG DG+G+ W L S+ + I + T+ ++T +S + +
Sbjct: 358 DVVITGDNGIIDGQGSVWWDLISTHSLNYSRPHI-IELVGSDNITISNLTFLNSPAWSIH 534
Query: 172 LLGCTNFTFDGFKIIAPGDSISINTDGIHIAKSTDVKILNSNIATGDDCVSLGDG----- 226
+ C+N + AP + T GI S V I N NI+TG D + L G
Sbjct: 535 PVYCSNVQIQKITVQAP--TKFPYTSGIVPDSSEHVCIDNCNISTGHDAIVLKSGWDEYG 708
Query: 227 --------NKHITIHNVKCGPGHGFSIGSLGTNPTEEPVENIIVKNCTLTNTQNGVRIKT 278
N HI ++ G G + GS + +II + +TN+ G+ +KT
Sbjct: 709 VAYGKPTSNVHIRGVYLQSSSGAGLAFGS----EMSGGISDIIAEQLHITNSTFGIELKT 876
Query: 279 WPTAPGTSPVTNLTFEDITMVNVYNPVIIDQQYCPSNLCSKQSPSKI-KISNVTFKNIKG 337
T + N+ D + N+Y + + S+ K P+ + + NVTF+N+ G
Sbjct: 877 --TRGRGGYMKNIFISDAKLENIYLGISMTGS-SGSHPDDKYDPNAVPDVGNVTFENVIG 1047
Query: 338 TS 339
+
Sbjct: 1048AN 1053
>TC209841
Length = 1087
Score = 50.1 bits (118), Expect = 2e-06
Identities = 50/198 (25%), Positives = 88/198 (44%), Gaps = 8/198 (4%)
Frame = +1
Query: 157 VRSVTSKDSKFFHVTLLGCTNFTFDGFKIIAPGDSISINTDGIHIAKSTDVKILNSNIAT 216
+ +VT +S F+ + + C++ T IIAP S NTDGI+ S +V I + I+T
Sbjct: 19 ISNVTFMNSPFWTIHPVYCSHVTIQNVTIIAPLSSP--NTDGINPDSSDNVCIEDCYIST 192
Query: 217 GDDCVSLGDG--------NKHITIHNVKCGPGHGFSIGSLGTNPTEEPVENIIVKNCTLT 268
GDD +S+ G + T N++ G S G + V + ++ +
Sbjct: 193 GDDLISIKSGWDGYGILFGRPSTNINIRRLIGKTTSAGIAIGSEMSGGVSEVHAEDIYIF 372
Query: 269 NTQNGVRIKTWPTAPGTSPVTNLTFEDITMVNVYNPVIIDQQYCPSNLCSKQSPSKIKIS 328
++ + +RIKT P G V N+ ++ +VNV + Y + + I
Sbjct: 373 DSHSAIRIKTSPGRGGY--VRNVYISNMILVNVDIAIRFTGLYGEHPDDTYDPDALPVIE 546
Query: 329 NVTFKNIKGTSETKDGVI 346
+T K++ G + G+I
Sbjct: 547 RITIKDVIGEKVKRAGLI 600
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.317 0.133 0.400
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 17,843,756
Number of Sequences: 63676
Number of extensions: 244954
Number of successful extensions: 1210
Number of sequences better than 10.0: 50
Number of HSP's better than 10.0 without gapping: 1157
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1168
length of query: 392
length of database: 12,639,632
effective HSP length: 99
effective length of query: 293
effective length of database: 6,335,708
effective search space: 1856362444
effective search space used: 1856362444
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 60 (27.7 bits)
Lotus: description of TM0490.17