BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0487.13
(394 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC213978 160 7e-40
TC233295 similar to UP|Q7KVQ0 (Q7KVQ0) CG4038-PA, partial (6%) 110 8e-25
AW756593 54 2e-07
AW596916 49 3e-06
TC222844 weakly similar to UP|Q9FFW4 (Q9FFW4) Similarity to heat... 49 4e-06
AW203333 49 5e-06
BM525658 44 2e-04
BU544682 42 4e-04
BU965508 42 6e-04
TC224116 42 6e-04
TC230253 40 0.002
BU084251 37 0.020
TC210377 similar to UP|Q8VAG3 (Q8VAG3) Wsv453 (WSSV513), partial... 34 0.13
TC213444 33 0.17
BU546502 33 0.29
BM886302 31 0.85
BM308009 similar to PIR|D86241|D862 protein T16B5.8 [imported] -... 30 1.4
TC234412 similar to UP|Q9LTZ9 (Q9LTZ9) Arabidopsis thaliana geno... 30 1.9
AI736087 homologue to GP|21427473|gb| actin-related protein 8A {... 30 1.9
TC227808 similar to UP|Q9SFU7 (Q9SFU7) T1B9.17 protein (AT3g0717... 30 2.5
>TC213978
Length = 716
Score = 160 bits (406), Expect = 7e-40
Identities = 105/239 (43%), Positives = 137/239 (56%), Gaps = 8/239 (3%)
Frame = +1
Query: 139 SMFSCTTLVVLKLEG----LELKANFSSVDLPFLKVLHLQDLLLQDEGCLAEILSGCLAL 194
++FSC TLVVLKL G +F SVDLP L LHLQ +L+ +AE+L G L
Sbjct: 22 ALFSCKTLVVLKLIGGLNRNPFPLDFKSVDLPLLTTLHLQSFILERRD-MAELLRGSPNL 198
Query: 195 EDLKARDVFFDGNKADAEFITLPKLVRADISESGGSQHFMMKVVNNVSFLRI----HEID 250
E L ++F G +A F LPKL+RA I+ H ++VVNNV FLRI H+ +
Sbjct: 199 EYLFVGHMYFSG--PEARFERLPKLLRATIAFG----HVPLEVVNNVQFLRIDWMEHKEE 360
Query: 251 YNLIYMGEDMFHNLTHLELVYTTFNRDWFEVLEFLKYCPKLEVLVIKQPQFYNVYLNKLG 310
NLI F NLTHLEL Y+ RDW +VLE ++ CP L++L I + G
Sbjct: 361 ANLI----PEFQNLTHLELGYSECTRDWVDVLEVIQRCPNLQILDIDMGSIDMTTRDDEG 528
Query: 311 AKDWQYPSSVPECILLHLKECCLNHYRGTKGELQFAKYIMEHGRLLNKMTICSSTAEKQ 369
A DW +P SVP I LHLK C + Y G+KGEL+FA+YIM + R L M I + + +Q
Sbjct: 529 A-DWPFPRSVPSSISLHLKTCFIRCYGGSKGELRFARYIMRNARHLRTMKISTYASRQQ 702
>TC233295 similar to UP|Q7KVQ0 (Q7KVQ0) CG4038-PA, partial (6%)
Length = 557
Score = 110 bits (276), Expect = 8e-25
Identities = 74/172 (43%), Positives = 96/172 (55%), Gaps = 3/172 (1%)
Frame = +3
Query: 7 EDRFSSLPDPILCHILSLLTTKEAVTTSILSKRWIPLWRSVPTLDFDD--SNLYGGDEVY 64
+DR S LPD +L ILSLL TK+AV T ILSKRW PLW +V LDFDD S +
Sbjct: 63 DDRISHLPDVLLLQILSLLPTKQAVITGILSKRWRPLWPAVSVLDFDDESSPEFHHPGGL 242
Query: 65 ARFVEAVYKVILARDFNQPIKKFRLVLTERLKDPANISVWVNLVLKRRVEHIDISLHFED 124
F E VY V+L D I++FRL +I+ W+ V +RR E +++SL
Sbjct: 243 TGFAEFVYSVLLLHD-APAIERFRLRCANPNYSARDIATWLCHVARRRAERVELSLSL-- 413
Query: 125 DEFLELPHIVVDTPSMFSCTTLVVLKLEGLELKANFS-SVDLPFLKVLHLQD 175
++ LP +F C T+ V+KL G+ L A S SV LP LKVLH+ D
Sbjct: 414 SRYVALPR------CLFHCDTVSVMKLNGVFLNALASFSVSLPLLKVLHVGD 551
>AW756593
Length = 370
Score = 53.5 bits (127), Expect = 2e-07
Identities = 24/48 (50%), Positives = 33/48 (68%)
Frame = +3
Query: 8 DRFSSLPDPILCHILSLLTTKEAVTTSILSKRWIPLWRSVPTLDFDDS 55
D+ S LPD IL H++ + T+EAV T +LSKRW LW+ + TL F+ S
Sbjct: 63 DKISELPDNILLHMMDFMDTREAVQTCVLSKRWNNLWKRLSTLLFNTS 206
>AW596916
Length = 455
Score = 49.3 bits (116), Expect = 3e-06
Identities = 22/49 (44%), Positives = 30/49 (60%)
Frame = +3
Query: 8 DRFSSLPDPILCHILSLLTTKEAVTTSILSKRWIPLWRSVPTLDFDDSN 56
DR S LPD +L HI+ ++ K AV T +LSKRW LW+ + L S+
Sbjct: 264 DRLSDLPDFVLLHIMKFMSMKHAVQTCVLSKRWKELWKRLTNLALHSSD 410
>TC222844 weakly similar to UP|Q9FFW4 (Q9FFW4) Similarity to heat shock
transcription factor HSF30, partial (6%)
Length = 746
Score = 48.9 bits (115), Expect = 4e-06
Identities = 29/78 (37%), Positives = 45/78 (57%), Gaps = 1/78 (1%)
Frame = +1
Query: 8 DRFSSLPDPILCHILSLLTTKEAVTTSILSKRWIPLWRSVPTLDFD-DSNLYGGDEVYAR 66
DR S+LPD +L H+++ + TK AV T +LSKRW L + + L F+ D G D + +
Sbjct: 496 DRISALPDSLLFHMINFMDTKSAVQTCVLSKRWNDLSKCLTNLTFNSDLPSEGLDRSFKK 675
Query: 67 FVEAVYKVILARDFNQPI 84
F V+ +RD + P+
Sbjct: 676 FES---WVLSSRDDSYPL 720
>AW203333
Length = 418
Score = 48.5 bits (114), Expect = 5e-06
Identities = 36/110 (32%), Positives = 57/110 (51%), Gaps = 10/110 (9%)
Frame = +1
Query: 8 DRFSSLPDPILCHILSLLTTKEAVTTSILSKRWIPLWRSVPTLDFDDSNLYGGD----EV 63
D S+LP IL ILS + ++AV TS+LSK W W + P L F D+ + G E
Sbjct: 85 DMISTLPKTILHDILSRMPEEDAVRTSVLSKSWAEAWSTYPILYFSDTMIVGTFPRPWED 264
Query: 64 YAR----FVEAVYKVILA-RDFNQPIKKFRLVLTERLKDPA-NISVWVNL 107
+ R F++ V + +L IK+FRL++ L+ + ++ W+ L
Sbjct: 265 FLRKRKNFIDHVKRTLLRFHTQGLAIKQFRLIINFDLQYMSLDVDHWLKL 414
>BM525658
Length = 431
Score = 43.5 bits (101), Expect = 2e-04
Identities = 19/36 (52%), Positives = 24/36 (65%), Gaps = 1/36 (2%)
Frame = +2
Query: 313 DWQYPSSVPECILLHLKECCLNHYRGTKG-ELQFAK 347
DW +P SVP CI LHLK C + Y G+K E++F K
Sbjct: 293 DWPFPRSVPSCISLHLKTCFIRCYGGSKALEIEFIK 400
>BU544682
Length = 475
Score = 42.4 bits (98), Expect = 4e-04
Identities = 23/82 (28%), Positives = 41/82 (49%), Gaps = 1/82 (1%)
Frame = -2
Query: 312 KDWQYPSS-VPECILLHLKECCLNHYRGTKGELQFAKYIMEHGRLLNKMTICSSTAEKQG 370
+ W P++ VP CI HL Y G+K EL+ KYI+++ ++L +T+ + +
Sbjct: 450 RKWTQPTAPVPSCISSHLTAFKFREYGGSKYELELTKYILKNAKVLKTVTVKMAKWLEPN 271
Query: 371 EKLENLKKLFSCTRCSATCKFS 392
+ + KL S S C+ +
Sbjct: 270 KTSHAMTKLDSLLSSSKYCRLA 205
>BU965508
Length = 447
Score = 41.6 bits (96), Expect = 6e-04
Identities = 22/47 (46%), Positives = 27/47 (56%)
Frame = +2
Query: 8 DRFSSLPDPILCHILSLLTTKEAVTTSILSKRWIPLWRSVPTLDFDD 54
D S LP I+ IL L ++AV TSILS +W W S+ L FDD
Sbjct: 296 DLISDLPQSIIESILVQLPIRDAVRTSILSSKWRYKWASITRLVFDD 436
>TC224116
Length = 520
Score = 41.6 bits (96), Expect = 6e-04
Identities = 22/47 (46%), Positives = 27/47 (56%)
Frame = +2
Query: 8 DRFSSLPDPILCHILSLLTTKEAVTTSILSKRWIPLWRSVPTLDFDD 54
D S LP I+ IL L ++AV TSILS +W W S+ L FDD
Sbjct: 287 DLISDLPQSIIESILVQLPIRDAVRTSILSSKWRYKWASITQLVFDD 427
>TC230253
Length = 1217
Score = 39.7 bits (91), Expect = 0.002
Identities = 25/83 (30%), Positives = 43/83 (51%), Gaps = 6/83 (7%)
Frame = +1
Query: 285 LKYCPKLEVLVIKQP------QFYNVYLNKLGAKDWQYPSSVPECILLHLKECCLNHYRG 338
LK CPKL+ L I +Y+ + ++ A ++ ECI LK + + G
Sbjct: 631 LKACPKLQNLEINSEINDNAVDYYDQH-DEDDAMEYYRRKEPCECIDQQLKTLSIMGFAG 807
Query: 339 TKGELQFAKYIMEHGRLLNKMTI 361
K E++F KY++ +G ++ K+TI
Sbjct: 808 KKTEVEFLKYVITNGEVMKKITI 876
>BU084251
Length = 409
Score = 36.6 bits (83), Expect = 0.020
Identities = 30/108 (27%), Positives = 54/108 (49%), Gaps = 2/108 (1%)
Frame = -3
Query: 281 VLEFLKYCPKLEVLVIKQPQFYNVYLNKLGAKDWQYPSSVPECILLHLKECCLNHYRGTK 340
+L L P L LV+K +++L KD +SVP+C L+ + +
Sbjct: 344 LLNILHNSPFLNTLVLKNG------VSELN-KDVLTSASVPQCFQSSLRVVQFKEFNVHE 186
Query: 341 GELQFAKYIMEHGRLLNKMTICSSTAEKQGE-KLENLK-KLFSCTRCS 386
ELQ K++M + ++L KMTI ++ + + +E +K ++ S +CS
Sbjct: 185 HELQILKFVMANAKVLEKMTIYTAFWLRYSDTDMEKVKEQILSFPKCS 42
>TC210377 similar to UP|Q8VAG3 (Q8VAG3) Wsv453 (WSSV513), partial (10%)
Length = 1077
Score = 33.9 bits (76), Expect = 0.13
Identities = 55/206 (26%), Positives = 83/206 (39%), Gaps = 13/206 (6%)
Frame = +3
Query: 2 KKMKMEDRFSS--LPDPILCHILSLLTTKEAVTTSIL-SKRWIPLWRSVPTLDFDDSNLY 58
K+ + E R + LP+ +L I+ + TK +V T +L SKRW L + + +L F + +
Sbjct: 255 KRQRTEKRRGTGDLPNCVLLQIMEFMNTKYSVQTCVLLSKRWKDL*KYLTSLSF---SRF 425
Query: 59 GGDEVYARFVEAVYKVILARDFNQPIKKFRLVLTERLKDPANISV----WV----NLVLK 110
D + R + L F L E + P I + W NL+ K
Sbjct: 426 QRDNLCGR---GYFITNLCLGF---------CLVEMVPSPFLILISLGRWFAGAPNLLNK 569
Query: 111 RRVEHIDISLHFEDDEFLELPHIVVDTPSMFSCTTLVVLKLEGLELKANFSSVDLPFLKV 170
+ + + I + D F P +F C L LKL S+ P LK
Sbjct: 570 QNTQRLAIDADYIPDCFF---------PLIFCCQFLTFLKLSIYSHLP--KSLQFPALKS 716
Query: 171 LHLQDL--LLQDEGCLAEILSGCLAL 194
LHL ++ D C AE S C +L
Sbjct: 717 LHLVNVGFTAIDRSC-AEPFSTCNSL 791
>TC213444
Length = 530
Score = 33.5 bits (75), Expect = 0.17
Identities = 14/25 (56%), Positives = 19/25 (76%)
Frame = +2
Query: 7 EDRFSSLPDPILCHILSLLTTKEAV 31
EDR S +PD I+ HILS + TK+A+
Sbjct: 455 EDRLSDMPDCIIHHILSFMETKDAI 529
>BU546502
Length = 516
Score = 32.7 bits (73), Expect = 0.29
Identities = 15/38 (39%), Positives = 21/38 (54%)
Frame = -1
Query: 314 WQYPSSVPECILLHLKECCLNHYRGTKGELQFAKYIME 351
W Y PECI L++C + +Y+ +L F KYI E
Sbjct: 387 WYYTEYGPECISS*LRKCTVLNYKDKVCKLLFVKYIAE 274
>BM886302
Length = 421
Score = 31.2 bits (69), Expect = 0.85
Identities = 13/35 (37%), Positives = 20/35 (57%)
Frame = +2
Query: 11 SSLPDPILCHILSLLTTKEAVTTSILSKRWIPLWR 45
SSLP P +CH+L+ + V + S+R + WR
Sbjct: 8 SSLPPPSVCHVLASVVLACVVVPLLSSRRCVSCWR 112
>BM308009 similar to PIR|D86241|D862 protein T16B5.8 [imported] - Arabidopsis
thaliana, partial (16%)
Length = 428
Score = 30.4 bits (67), Expect = 1.4
Identities = 24/71 (33%), Positives = 34/71 (47%), Gaps = 8/71 (11%)
Frame = +3
Query: 12 SLPDPILCHILSLLTTKEAVTT-SILSKRWIPLWRSVPTLDF-------DDSNLYGGDEV 63
S+PD IL ILS +T V++ + +SKRW + TL F S+ G D +
Sbjct: 36 SMPDAILQCILSRITNARDVSSCNCVSKRWKDSTPYIRTLYFPRSSFETPSSSSEGADNI 215
Query: 64 YARFVEAVYKV 74
R V V K+
Sbjct: 216 IKRMVSRVVKL 248
>TC234412 similar to UP|Q9LTZ9 (Q9LTZ9) Arabidopsis thaliana genomic DNA,
chromosome 5, TAC clone:K15C23 (AT5g44670/K15C23_12),
partial (16%)
Length = 606
Score = 30.0 bits (66), Expect = 1.9
Identities = 14/36 (38%), Positives = 20/36 (54%), Gaps = 6/36 (16%)
Frame = -2
Query: 307 NKLGAKDWQYPSSVPECILLHLKE------CCLNHY 336
N L K YPSSVP C + H+++ CC++ Y
Sbjct: 374 NHLELKFLDYPSSVPHCGVHHIRDFVLGDLCCVDEY 267
>AI736087 homologue to GP|21427473|gb| actin-related protein 8A {Arabidopsis
thaliana}, partial (13%)
Length = 427
Score = 30.0 bits (66), Expect = 1.9
Identities = 16/31 (51%), Positives = 18/31 (57%)
Frame = +1
Query: 13 LPDPILCHILSLLTTKEAVTTSILSKRWIPL 43
LP IL HIL LL KEA S++ K PL
Sbjct: 103 LPSDILMHILRLLGPKEAAKLSVVCKALRPL 195
>TC227808 similar to UP|Q9SFU7 (Q9SFU7) T1B9.17 protein (AT3g07170/T1B9_17),
partial (53%)
Length = 1017
Score = 29.6 bits (65), Expect = 2.5
Identities = 18/45 (40%), Positives = 24/45 (53%), Gaps = 1/45 (2%)
Frame = -2
Query: 127 FLELPHIVVDTPSMFSCTTLVV-LKLEGLELKANFSSVDLPFLKV 170
F+E+PH+ T S SC + LKLE NF+ DL F+ V
Sbjct: 500 FMEIPHVWSRTLSTLSCKLKTLPLKLEAKIWSTNFAISDLRFVIV 366
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.324 0.140 0.423
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 19,548,795
Number of Sequences: 63676
Number of extensions: 286188
Number of successful extensions: 2070
Number of sequences better than 10.0: 46
Number of HSP's better than 10.0 without gapping: 2053
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2065
length of query: 394
length of database: 12,639,632
effective HSP length: 99
effective length of query: 295
effective length of database: 6,335,708
effective search space: 1869033860
effective search space used: 1869033860
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.5 bits)
S2: 60 (27.7 bits)
Lotus: description of TM0487.13