BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0476b.11
(556 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BG882562 102 4e-22
BM892478 100 1e-21
TC220316 98 1e-20
AW781991 88 1e-17
AW569215 weakly similar to GP|4914326|gb| F14N23.12 {Arabidopsis... 82 5e-16
BI973310 75 1e-13
BM178397 similar to GP|4914326|gb| F14N23.12 {Arabidopsis thalia... 65 1e-10
TC228242 weakly similar to UP|Q9LIE5 (Q9LIE5) Far-red impaired r... 46 4e-05
TC208963 44 2e-04
BI424115 similar to GP|15982769|gb| At2g27110/T20P8.16 {Arabidop... 42 7e-04
BM309807 similar to GP|5764395|gb| far-red impaired response pro... 40 0.002
TC210791 38 0.014
TC233004 weakly similar to PIR|G96565|G96565 F6D8.26 [imported] ... 33 0.33
TC207649 similar to UP|Q9SY66 (Q9SY66) F14N23.12 (At1g10240/F14N... 31 1.7
TC234242 30 2.8
TC207298 30 3.7
CF807827 29 4.8
TC220897 homologue to UP|GBA2_SOYBN (P93163) Guanine nucleotide-... 29 4.8
>BG882562
Length = 416
Score = 102 bits (254), Expect = 4e-22
Identities = 50/137 (36%), Positives = 73/137 (52%)
Frame = +3
Query: 166 ELFAASAGDAASALSYLQRKGEKDPMFFFKFTRSGEENLENLFWCDGTSRLDYQAFGDVI 225
+ F GD +Y R +P FF+ + + L N+FW D SR Y FGDV+
Sbjct: 6 QAFET*KGDPELISNYFCRIQLMNPNFFYVMDLNDDGQLRNVFWIDSRSRAAYSYFGDVV 185
Query: 226 VFYSTYKKNKYNKPVVIFSGYNHHKETTIFACALVCDETIETYKWVLKALDEAMFGKQPK 285
F ST N Y P+V F G NHH ++ + C L+ DET ETY W+ +A M G+ P+
Sbjct: 186 AFDSTCLSNNYEIPLVAFVGVNHHGKSVLLGCGLLADETFETYIWLFRAWLTCMTGRPPQ 365
Query: 286 AVVTDGDKSMREAVKVV 302
++T+ K+M+ A+ V
Sbjct: 366 TIITNXCKAMQSAIAEV 416
>BM892478
Length = 429
Score = 100 bits (250), Expect = 1e-21
Identities = 49/131 (37%), Positives = 71/131 (53%)
Frame = +3
Query: 179 LSYLQRKGEKDPMFFFKFTRSGEENLENLFWCDGTSRLDYQAFGDVIVFYSTYKKNKYNK 238
L Y Q + +D FF+ N N+FW DG SR FGDV+V ++Y+K Y
Sbjct: 33 LEYFQSRQAEDTGFFYAM-EVDNGNCMNIFWADGRSRYSCSHFGDVLVLDTSYRKTVYLV 209
Query: 239 PVVIFSGYNHHKETTIFACALVCDETIETYKWVLKALDEAMFGKQPKAVVTDGDKSMREA 298
P F G NHHK+ + CAL+ DE+ E++ W+ + AM G+ P V+ D D +++ A
Sbjct: 210 PFATFVGVNHHKQPVLLGCALIADESEESFTWLFQTWLRAMSGRLPLTVIADQDIAIQRA 389
Query: 299 VKVVFPNATHR 309
+ VFP HR
Sbjct: 390 IAKVFPVTHHR 422
>TC220316
Length = 988
Score = 97.8 bits (242), Expect = 1e-20
Identities = 52/219 (23%), Positives = 101/219 (45%)
Frame = +1
Query: 337 YGNFTPERFETKWLQVIEKYGIGEEKWIKQTYETRQMWATAFMREKFFAGIRTTSLCEGI 396
Y + T + W KYG+ ++ W+K+ Y+ R W +++ FFAGI + E +
Sbjct: 10 YQSQTVDEXXATWNXXXNKYGLKDDAWLKEMYQKRASWVPLYLKGTFFAGI---PMNESL 180
Query: 397 NSFIKRYVQCKNSILDFIYNFERAVEEYRHNELASDFKSSYGEPVLITALSHIEQGAAKL 456
+SF + + +++FI +ER +E R E DF +S +P+L T +E+ +L
Sbjct: 181 DSFFGALLNAQTPLMEFIPRYERGLERRREEERKEDFNTSNFQPILQTK-EPVEEQCRRL 357
Query: 457 LTLNMFREVRHMIQRALNLNLVERSEIGTTVMIKFSICRHPNTQFLVIYDKNQNMFDCDC 516
TL +F+ + + + + + E G C + + +V ++ + C C
Sbjct: 358 YTLTVFKIFQKELLQCFSYLGFKIFEEGGLSRYMVRRCGNDMEKHVVTFNASNISISCSC 537
Query: 517 GFSEYVGIPCSHIICAMRTENMNEFPASLVSKRWLKTAK 555
EY G+ C H++ + + E P+ + RW + +
Sbjct: 538 QMFEYEGVLCRHVLRVFQILQLREVPSRYILHRWTRNTE 654
>AW781991
Length = 419
Score = 87.8 bits (216), Expect = 1e-17
Identities = 34/101 (33%), Positives = 60/101 (58%)
Frame = +3
Query: 173 GDAASALSYLQRKGEKDPMFFFKFTRSGEENLENLFWCDGTSRLDYQAFGDVIVFYSTYK 232
GD L YL++ ++P FF+ ++++ N+FW D +R++Y FGD + F +TY+
Sbjct: 111 GDIQLVLDYLRQMHAENPNFFYAVQGDEDQSITNVFWADPKARMNYTFFGDTVTFDTTYR 290
Query: 233 KNKYNKPVVIFSGYNHHKETTIFACALVCDETIETYKWVLK 273
N+Y P F+G NHH + +F CA + +E+ ++ W+ K
Sbjct: 291 SNRYRLPFAFFTGVNHHGQPVLFGCAFLINESEASFVWLFK 413
>AW569215 weakly similar to GP|4914326|gb| F14N23.12 {Arabidopsis thaliana},
partial (19%)
Length = 425
Score = 82.4 bits (202), Expect = 5e-16
Identities = 44/138 (31%), Positives = 75/138 (53%), Gaps = 5/138 (3%)
Frame = +1
Query: 225 IVFYSTYKKNKYNKPVVIFSGYNHHKETTIFACALVCDETIETYKWVLKALDEAMFGKQP 284
+VF +TY+ Y+ + I+ G +++ T F+CAL+ DE I+++ W LKA M GK P
Sbjct: 10 VVFDTTYRVEAYDMLLGIWLGVDNNGMTCFFSCALLRDENIQSFSWALKAFLGFMKGKAP 189
Query: 285 KAVVTDGDKSMREAVKVVFPNATHRLCGWHIQQNCLE--KIKIPDFLNEFKT---LIYGN 339
+ ++TD + ++EA+ V P H C WHI + + + +E+K +Y
Sbjct: 190 QTILTDHNMWLKEAIAVELPQTKHAFCIWHILSKFSDWFSLLLGSQYDEWKAEFHRLYNL 369
Query: 340 FTPERFETKWLQVIEKYG 357
E FE W Q++++YG
Sbjct: 370 EQVEDFEEGWRQMVDQYG 423
>BI973310
Length = 457
Score = 74.7 bits (182), Expect = 1e-13
Identities = 40/145 (27%), Positives = 74/145 (50%)
Frame = +3
Query: 341 TPERFETKWLQVIEKYGIGEEKWIKQTYETRQMWATAFMREKFFAGIRTTSLCEGINSFI 400
T + FE+ W ++E++ + + +W++ Y +RQ W ++RE FF I E + SF
Sbjct: 3 TIDEFESYWHSLLERFYVMDNEWLQSIYNSRQHWVPVYLRETFFGEISLNEGNEYLISFF 182
Query: 401 KRYVQCKNSILDFIYNFERAVEEYRHNELASDFKSSYGEPVLITALSHIEQGAAKLLTLN 460
YV ++ + +E+AV + EL +D+ ++ PVL T S +E+ AA L T
Sbjct: 183 DGYVFSSTTLQVLVRQYEKAVSSWHEKELKADYDTTNSSPVLKTP-SPMEKQAASLYTRK 359
Query: 461 MFREVRHMIQRALNLNLVERSEIGT 485
+F + + + L ++ + GT
Sbjct: 360 IFMKFQEELVETLANPAIKIDDSGT 434
>BM178397 similar to GP|4914326|gb| F14N23.12 {Arabidopsis thaliana}, partial
(20%)
Length = 422
Score = 64.7 bits (156), Expect = 1e-10
Identities = 37/134 (27%), Positives = 63/134 (46%), Gaps = 6/134 (4%)
Frame = +1
Query: 295 MREAVKVVFPNATHRLCGWHIQQ------NCLEKIKIPDFLNEFKTLIYGNFTPERFETK 348
++EA+ P H C W I N + + D+ EF L Y + E FE
Sbjct: 13 LKEALSTEMPTTKHAFCIWMIVAKFPSWFNAVLGERYNDWKAEFYRL-YNLESVEDFELG 189
Query: 349 WLQVIEKYGIGEEKWIKQTYETRQMWATAFMREKFFAGIRTTSLCEGINSFIKRYVQCKN 408
W ++ +G+ + + Y +R +WA F+R F AG+ TT + IN+FI+R++ +
Sbjct: 190 WREMACSFGLHSNRHMVNLYSSRSLWALPFLRSHFLAGMTTTGQSKSINAFIQRFLSAQT 369
Query: 409 SILDFIYNFERAVE 422
+ F+ AV+
Sbjct: 370 RLAHFVEQVAVAVD 411
>TC228242 weakly similar to UP|Q9LIE5 (Q9LIE5) Far-red impaired response
protein; Mutator-like transposase-like protein;
phytochrome A signaling protein-like, partial (25%)
Length = 1354
Score = 46.2 bits (108), Expect = 4e-05
Identities = 26/109 (23%), Positives = 52/109 (46%)
Frame = +2
Query: 447 SHIEQGAAKLLTLNMFREVRHMIQRALNLNLVERSEIGTTVMIKFSICRHPNTQFLVIYD 506
S +E+ A + T +F++++ + A+ + + TT++ + N F V+ +
Sbjct: 44 SPLEKSVAGIFTHAVFKKIQAEVIGAVACHPKADRQDDTTIVHRVHDME-TNKDFFVVVN 220
Query: 507 KNQNMFDCDCGFSEYVGIPCSHIICAMRTENMNEFPASLVSKRWLKTAK 555
+ ++ C C EY G C H + ++ + FP+ + KRW K AK
Sbjct: 221 QVKSELSCICRLFEYRGYLCRHALFVLQYSGQSVFPSQYILKRWTKDAK 367
>TC208963
Length = 1163
Score = 43.9 bits (102), Expect = 2e-04
Identities = 22/53 (41%), Positives = 34/53 (63%)
Frame = +1
Query: 284 PKAVVTDGDKSMREAVKVVFPNATHRLCGWHIQQNCLEKIKIPDFLNEFKTLI 336
P+ +VT GD ++ AV+VVFP++++ LC +HI QN K K L E + L+
Sbjct: 22 PQVIVTVGDIALMSAVQVVFPSSSNLLCRFHINQNVKAKCKSIVHLKEKQELM 180
>BI424115 similar to GP|15982769|gb| At2g27110/T20P8.16 {Arabidopsis
thaliana}, partial (9%)
Length = 402
Score = 42.0 bits (97), Expect = 7e-04
Identities = 17/55 (30%), Positives = 27/55 (48%)
Frame = +2
Query: 501 FLVIYDKNQNMFDCDCGFSEYVGIPCSHIICAMRTENMNEFPASLVSKRWLKTAK 555
++V + ++ +C C EY GI C HI+ N+ P + KRW + AK
Sbjct: 50 YMVTLNHSELKANCSCQMFEYAGILCKHILTVFTVTNVLTLPPHYILKRWTRNAK 214
>BM309807 similar to GP|5764395|gb| far-red impaired response protein
{Arabidopsis thaliana}, partial (15%)
Length = 428
Score = 40.4 bits (93), Expect = 0.002
Identities = 22/77 (28%), Positives = 37/77 (47%), Gaps = 1/77 (1%)
Frame = +3
Query: 480 RSEIGTTVMIKFSICRHP-NTQFLVIYDKNQNMFDCDCGFSEYVGIPCSHIICAMRTENM 538
R E G + KF + + + +FLV +++ + C C EY G C H + ++
Sbjct: 132 RIEAGDGTIAKFIVQDYEKDEEFLVTWNELSSEVSCFCRLFEYKGFLCRHALSVLQRCGC 311
Query: 539 NEFPASLVSKRWLKTAK 555
+ P+ + KRW K AK
Sbjct: 312 SCVPSHYILKRWTKDAK 362
>TC210791
Length = 456
Score = 37.7 bits (86), Expect = 0.014
Identities = 15/44 (34%), Positives = 30/44 (68%)
Frame = +1
Query: 282 KQPKAVVTDGDKSMREAVKVVFPNATHRLCGWHIQQNCLEKIKI 325
+ P ++T D ++ +AV+VVFP++++ LC +HI +N K ++
Sbjct: 25 EMPPVIITVRDIALMDAVQVVFPSSSNLLCRFHISKNVKAKSQV 156
>TC233004 weakly similar to PIR|G96565|G96565 F6D8.26 [imported] -
Arabidopsis thaliana {Arabidopsis thaliana;} , partial
(8%)
Length = 551
Score = 33.1 bits (74), Expect = 0.33
Identities = 31/160 (19%), Positives = 61/160 (37%), Gaps = 7/160 (4%)
Frame = +1
Query: 403 YVQCKNSILDFIYNFERAVEEYRHNELASDFKSSYGEPVLITALSHIEQGAAKLLTLNMF 462
YV S +F+ ++ + E +D ++ L T + E AK+ T +F
Sbjct: 7 YVHKHTSFKEFVDKYDLVLHRKHLKEAMADLETRNVSFELKTRCN-FEVQLAKVYTKEIF 183
Query: 463 REVRHMIQ---RALNLNLVERSEIGTTVMIKFSICRHPNTQ----FLVIYDKNQNMFDCD 515
++ + ++ N V + T ++K + N + F V+Y+ + C
Sbjct: 184 QKFQSEVEGMYSCFNTRQVSVNGSIITYVVKERVEVEGNEKGVKSFEVLYETTELDIRCI 363
Query: 516 CGFSEYVGIPCSHIICAMRTENMNEFPASLVSKRWLKTAK 555
C Y G C + + + E P+ + RW + K
Sbjct: 364 CSLFNYKGYLCRRALNVLNYNGIEEIPSRYILHRWRRDFK 483
>TC207649 similar to UP|Q9SY66 (Q9SY66) F14N23.12 (At1g10240/F14N23_12),
partial (30%)
Length = 971
Score = 30.8 bits (68), Expect = 1.7
Identities = 13/48 (27%), Positives = 21/48 (43%)
Frame = +2
Query: 503 VIYDKNQNMFDCDCGFSEYVGIPCSHIICAMRTENMNEFPASLVSKRW 550
V + + + C C E+ GI C H + + T N + P + RW
Sbjct: 398 VYWAPQEGIISCSCHQFEFTGILCRHSLRVLSTGNCFQIPDRYLPIRW 541
>TC234242
Length = 478
Score = 30.0 bits (66), Expect = 2.8
Identities = 14/43 (32%), Positives = 23/43 (52%)
Frame = +3
Query: 201 EENLENLFWCDGTSRLDYQAFGDVIVFYSTYKKNKYNKPVVIF 243
E+ + ++FWC S A V + STY+ N+Y P++ F
Sbjct: 81 EDVVRDIFWCHPDSVKLVNACNLVFLIDSTYRTNRYRLPLLDF 209
>TC207298
Length = 1011
Score = 29.6 bits (65), Expect = 3.7
Identities = 11/28 (39%), Positives = 19/28 (67%)
Frame = +3
Query: 203 NLENLFWCDGTSRLDYQAFGDVIVFYST 230
++++ FW SR+D+ FGD++VF T
Sbjct: 342 HIQHTFWLICLSRVDWWQFGDLVVFQGT 425
>CF807827
Length = 642
Score = 29.3 bits (64), Expect = 4.8
Identities = 26/114 (22%), Positives = 49/114 (42%), Gaps = 7/114 (6%)
Frame = +3
Query: 286 AVVTDGDKSMREAVKVVFPNATHRLCGWHIQQNCLEKIKIPDFLNEFKTLIYGNFTPERF 345
A +++ D+++ E T L W ++Q C EK FLN+F
Sbjct: 138 AAISEYDQTLFEDENRNRMTETKELFEWILKQPCFEKTSFMLFLNKFDIFEKKILKVPLN 317
Query: 346 ETKWLQVIEKYGIGEE------KWIKQTYETRQMWATAFMR-EKFFAGIRTTSL 392
+W + + G++ +++K+ +E +TA R ++ F RTT+L
Sbjct: 318 VCEWFKDYQPVSTGKQEIEHAYEFVKKKFEESYFQSTAPDRVDRVFKIYRTTAL 479
>TC220897 homologue to UP|GBA2_SOYBN (P93163) Guanine nucleotide-binding
protein alpha-2 subunit (GP-alpha-2), partial (62%)
Length = 1123
Score = 29.3 bits (64), Expect = 4.8
Identities = 26/114 (22%), Positives = 49/114 (42%), Gaps = 7/114 (6%)
Frame = +2
Query: 286 AVVTDGDKSMREAVKVVFPNATHRLCGWHIQQNCLEKIKIPDFLNEFKTLIYGNFTPERF 345
A +++ D+++ E T L W ++Q C EK FLN+F
Sbjct: 299 AAISEYDQTLFEDENRNRMTETKELFEWILKQPCFEKTSFMLFLNKFDIFEKKILKVPLN 478
Query: 346 ETKWLQVIEKYGIGEE------KWIKQTYETRQMWATAFMR-EKFFAGIRTTSL 392
+W + + G++ +++K+ +E +TA R ++ F RTT+L
Sbjct: 479 VCEWFKDYQPVSTGKQEIEHAYEFVKKKFEESYFQSTAPDRVDRVFKIYRTTAL 640
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.336 0.144 0.474
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 26,223,335
Number of Sequences: 63676
Number of extensions: 371729
Number of successful extensions: 2713
Number of sequences better than 10.0: 36
Number of HSP's better than 10.0 without gapping: 2691
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2709
length of query: 556
length of database: 12,639,632
effective HSP length: 102
effective length of query: 454
effective length of database: 6,144,680
effective search space: 2789684720
effective search space used: 2789684720
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.7 bits)
S2: 61 (28.1 bits)
Lotus: description of TM0476b.11