BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0381.3
(253 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC211561 weakly similar to UP|O22675 (O22675) Reverse transcript... 130 4e-31
CD417230 weakly similar to GP|2244915|emb| reverse transcriptase... 106 9e-24
TC233727 similar to UP|Q9FKH8 (Q9FKH8) Similarity to non-LTR ret... 65 4e-23
TC212765 84 1e-18
CO982349 89 2e-18
AW757033 74 8e-14
BI315739 66 1e-11
TC233219 weakly similar to UP|Q8LKI9 (Q8LKI9) NBS/LRR resistance... 45 2e-05
BI972659 40 8e-04
BI471325 40 0.001
TC212055 36 0.015
TC234877 35 0.033
BI470652 similar to PIR|T17102|T171 RNA-directed DNA polymerase ... 33 0.16
AI496436 32 0.21
TC215144 similar to UP|ATPQ_ARATH (Q9FT52) ATP synthase D chain,... 28 4.0
AW101881 28 4.0
TC215146 similar to UP|ATPQ_ARATH (Q9FT52) ATP synthase D chain,... 28 4.0
TC229805 similar to PIR|H86466|H86466 protein F23M19.7 [imported... 27 6.8
BF070779 27 8.9
TC231912 similar to UP|Q93Y40 (Q93Y40) Oxysterol-binding protein... 27 8.9
>TC211561 weakly similar to UP|O22675 (O22675) Reverse transcriptase
(Fragment) , partial (59%)
Length = 1077
Score = 130 bits (328), Expect = 4e-31
Identities = 83/245 (33%), Positives = 129/245 (51%)
Frame = +1
Query: 9 ILAQEVMHTIHTQKKGRGLVAIKIDLEKAYDRVSWDFLRATLYDFGFPPRIVDLIMWGVK 68
++A EV+ ++ + + K+D EKAYD VSW+FL ++ F P+ + I VK
Sbjct: 13 LIANEVID--EAKRSNKSCLVFKVDYEKAYDSVSWNFLMYMMWRMDFSPKWIKWIEECVK 186
Query: 69 CPKLSILWNCSKLPSFAPQRGLRQGDPLSPYLFVLCMERLAIQIQNLVTQGTWKPVRITK 128
+S+L N S F PQRGLRQGDPL+P+LF + E L ++ V + +KP +
Sbjct: 187 SASISVLVNGSPTAEFLPQRGLRQGDPLTPFLFNIVAEGLNGLMRRAVEENLYKPYLVGA 366
Query: 129 DGVGISHLFFADDVLLFCQASKEQLQLVNNTLKEFCEASGMKVNLDKSRMLFSKAVDHRR 188
+GV IS L +ADD + F +A+KE ++ + L+ F S +++N KS D +
Sbjct: 367 NGVPISILQYADDTIFFGEAAKENVEAIKVILRSFELVSNLRINFAKSCFGVFGVTDQWK 546
Query: 189 QINLSHIAGISRASDLGKYLGIPLLKGRVTKAHFASLIGKMSSRLTSWKNNLLNRAGRVC 248
Q ++ S + YLGIP+ + S+I K +L+ WK ++ GRV
Sbjct: 547 Q-EAANYLHCSLLAFPFIYLGIPIGANSRRCHMWDSIIRKCERKLSKWK*RHISFGGRVT 723
Query: 249 LAKSV 253
L KSV
Sbjct: 724 LIKSV 738
>CD417230 weakly similar to GP|2244915|emb| reverse transcriptase like
protein {Arabidopsis thaliana}, partial (7%)
Length = 688
Score = 106 bits (265), Expect = 9e-24
Identities = 55/147 (37%), Positives = 93/147 (62%), Gaps = 1/147 (0%)
Frame = -3
Query: 60 VDLIMWGVKCPKLSILWNCSKLPSFAPQRGLRQGDPLSPYLFVLCMERLAIQIQNLVTQG 119
V L+ + + +S+LWN L F+P G+RQ DP++PYLFVLC+ERL+ I ++ +
Sbjct: 686 VQLVWYCMSTTTMSLLWNGEVLDHFSPNIGVRQRDPIAPYLFVLCLERLSHLINLVMNKK 507
Query: 120 TWKPVRITKDGVGISHLFFADDVLLFCQASKEQLQLVNNTLKEFCEASGMKVNLDKSR-M 178
+ +++ + G+ ISHL F DD++LF +A+ +Q+ ++N TL+ F ++SG KVN+DK++
Sbjct: 506 L*RSIQVNRGGLLISHLAFVDDLILFVEANMDQVDVINLTLELFSDSSGAKVNVDKTKSF 327
Query: 179 LFSKAVDHRRQINLSHIAGISRASDLG 205
LF K + I G+ + S +G
Sbjct: 326 LFPKML----------IGGLRKTSQVG 276
Score = 55.8 bits (133), Expect = 2e-08
Identities = 28/72 (38%), Positives = 42/72 (57%)
Frame = -2
Query: 180 FSKAVDHRRQINLSHIAGISRASDLGKYLGIPLLKGRVTKAHFASLIGKMSSRLTSWKNN 239
FSK V+ + ++S G+ DLGKYLG+P+ T F +I K++ R + WK+N
Sbjct: 324 FSKNVNWWVEEDISSGLGLQSTDDLGKYLGVPIFHKNPTSHTFDFIIDKVNKRFSRWKSN 145
Query: 240 LLNRAGRVCLAK 251
LL+ GR+ L K
Sbjct: 144 LLSMVGRLTLTK 109
>TC233727 similar to UP|Q9FKH8 (Q9FKH8) Similarity to non-LTR retroelement
reverse transcriptase, partial (7%)
Length = 956
Score = 64.7 bits (156), Expect(2) = 4e-23
Identities = 31/120 (25%), Positives = 63/120 (51%)
Frame = -2
Query: 134 SHLFFADDVLLFCQASKEQLQLVNNTLKEFCEASGMKVNLDKSRMLFSKAVDHRRQINLS 193
SH+ ADD+++FC+ + + + N + + ++ G ++ KS++ R ++ +S
Sbjct: 451 SHVMHADDIMIFCKGTSRNVHNLINFFQLYKDSFGQVISKHKSKIFIGSIPGQRLRV-IS 275
Query: 194 HIAGISRASDLGKYLGIPLLKGRVTKAHFASLIGKMSSRLTSWKNNLLNRAGRVCLAKSV 253
+ + Y G+P+ KG+ T+ H + K+ +L SWK ++L+ GR+ L SV
Sbjct: 274 DLLQYFHSDIPFNYFGVPIFKGKPTRRHLYCVADKIKCKLASWKGSILSIMGRIQLVNSV 95
Score = 60.5 bits (145), Expect(2) = 4e-23
Identities = 40/109 (36%), Positives = 62/109 (56%), Gaps = 1/109 (0%)
Frame = -3
Query: 5 RDNIILAQEVMHTIHTQKKGRGLVAIKIDLEKAYDRVSWDFLRATLYDFGFPPRIVDLIM 64
+D + EV++ + + G G +A+KID+ KA+D + DFL + L FG+ + I
Sbjct: 831 KDCTCVTSEVINMLDKKVFG-GNLALKIDIVKAFDTLDRDFLISVLNKFGYSHTFCNWIR 655
Query: 65 WGVKCPKLSILWNCSKLPSFAPQRGLRQGDPLSPYLF-VLCMERLAIQI 112
+ KLSI N + F+ QRG+RQGDPLSP L+ C+E+ I +
Sbjct: 654 VILLSAKLSISVNGEPVGFFSCQRGVRQGDPLSPSLYRQRCLEQGTIYV 508
>TC212765
Length = 637
Score = 84.3 bits (207), Expect(2) = 1e-18
Identities = 47/119 (39%), Positives = 68/119 (56%)
Frame = +3
Query: 98 PYLFVLCMERLAIQIQNLVTQGTWKPVRITKDGVGISHLFFADDVLLFCQASKEQLQLVN 157
PYLFVLCMERLA+ I +L +Q W+P+ ++ D + +L DD+ LF A + L +
Sbjct: 15 PYLFVLCMERLALCIHDLTSQ*IWRPILVSDDNRLVPYLMLVDDIFLFINAIGD*AYLAS 194
Query: 158 NTLKEFCEASGMKVNLDKSRMLFSKAVDHRRQINLSHIAGISRASDLGKYLGIPLLKGR 216
TL F + G+K+NLDKS M S + + +S+ I A LGKYL + + GR
Sbjct: 195 LTL*NFFQTFGLKLNLDKSSMYCS*RMAPVLRDLISNTLNIVHAPSLGKYLCLKFIHGR 371
Score = 25.8 bits (55), Expect(2) = 1e-18
Identities = 10/17 (58%), Positives = 12/17 (69%)
Frame = +2
Query: 233 LTSWKNNLLNRAGRVCL 249
L SWK LLN+A + CL
Sbjct: 416 LASWKGKLLNQASKHCL 466
>CO982349
Length = 795
Score = 89.0 bits (219), Expect = 2e-18
Identities = 59/182 (32%), Positives = 94/182 (51%)
Frame = +3
Query: 72 LSILWNCSKLPSFAPQRGLRQGDPLSPYLFVLCMERLAIQIQNLVTQGTWKPVRITKDGV 131
+SIL N S + F+PQRGLRQGDPL+P LF + E L ++ V + + + K+
Sbjct: 99 VSILVNESPINEFSPQRGLRQGDPLAPLLFNIVAEGLTGLMREAVDRKRFNSFLVGKNKE 278
Query: 132 GISHLFFADDVLLFCQASKEQLQLVNNTLKEFCEASGMKVNLDKSRMLFSKAVDHRRQIN 191
+S L +ADD + F +A+ E ++++ L+ F ASG+K+N +SR DH +
Sbjct: 279 PVSILQYADDTIFFEEATMENMKVIKTILRCFELASGLKINFAESRFGAIWKPDHWCK-E 455
Query: 192 LSHIAGISRASDLGKYLGIPLLKGRVTKAHFASLIGKMSSRLTSWKNNLLNRAGRVCLAK 251
+ S S YLGIP+ + +I K ++L WK ++ GRV L
Sbjct: 456 AAEFLNCSMLSMPFSYLGIPIEANPRCREI*DPIIRKCETKLARWKQRHISLGGRVTLIN 635
Query: 252 SV 253
++
Sbjct: 636 AI 641
>AW757033
Length = 441
Score = 73.6 bits (179), Expect = 8e-14
Identities = 46/129 (35%), Positives = 69/129 (52%)
Frame = -2
Query: 84 FAPQRGLRQGDPLSPYLFVLCMERLAIQIQNLVTQGTWKPVRITKDGVGISHLFFADDVL 143
F+P RGLRQGDPL+P LF + E L ++ V + +K + K +S L +ADD +
Sbjct: 431 FSPHRGLRQGDPLAPLLFNIAAEGLTGLMREAVARNHYKSFAVGKYKKPVSILQYADDTI 252
Query: 144 LFCQASKEQLQLVNNTLKEFCEASGMKVNLDKSRMLFSKAVDHRRQINLSHIAGISRASD 203
F +A+ E ++++ L+ F ASG+K+N KSR D R+ + S S
Sbjct: 251 FFGEATMENVRVIKTILRGFELASGLKINFAKSRFGAISVPD*WRK-EAAEFMNCSLLSL 75
Query: 204 LGKYLGIPL 212
YLGIP+
Sbjct: 74 PFSYLGIPI 48
>BI315739
Length = 442
Score = 65.9 bits (159), Expect(2) = 1e-11
Identities = 48/145 (33%), Positives = 78/145 (53%), Gaps = 1/145 (0%)
Frame = +3
Query: 95 PLSPYLFVLCMERLAIQIQNLVTQGTWKPVRITKDGVGISHLFFADDVLLFCQASKEQLQ 154
PLSPYLF +CME+LAI IQ+ V + TW+P++I+++G GIS ++ + ++ F + L
Sbjct: 48 PLSPYLF*ICMEKLAILIQSKVNEETWEPIKISRNGPGISPIYSSQIIVYFL---*KPLV 218
Query: 155 LVNNTLKEFCEASGMKVNLDKSRMLF-SKAVDHRRQINLSHIAGISRASDLGKYLGIPLL 213
L N + C + V + SR +F + H+R + + G L LL
Sbjct: 219 LKLNW*EMCCNSF---V*PEVSRRVFKNPGF*HKRMCLIEKLPG---------SLL**LL 362
Query: 214 KGRVTKAHFASLIGKMSSRLTSWKN 238
GR K FA ++ +++RL WK+
Sbjct: 363 AGRTKKEDFAHVVDMINNRLDGWKS 437
Score = 20.8 bits (42), Expect(2) = 1e-11
Identities = 9/14 (64%), Positives = 9/14 (64%)
Frame = +1
Query: 81 LPSFAPQRGLRQGD 94
L SF P LRQGD
Sbjct: 7 LESFTPTGDLRQGD 48
>TC233219 weakly similar to UP|Q8LKI9 (Q8LKI9) NBS/LRR resistance
protein-like protein (Fragment), partial (74%)
Length = 781
Score = 45.4 bits (106), Expect = 2e-05
Identities = 33/103 (32%), Positives = 49/103 (47%)
Frame = +3
Query: 89 GLRQGDPLSPYLFVLCMERLAIQIQNLVTQGTWKPVRITKDGVGISHLFFADDVLLFCQA 148
GL QG LSPYLF L ++ L QIQ +V++ + FADD++L +
Sbjct: 132 GLHQGSTLSPYLFTLILDVLTEQIQEIVSR----------------CMLFADDIVLLGE- 260
Query: 149 SKEQLQLVNNTLKEFCEASGMKVNLDKSRMLFSKAVDHRRQIN 191
S+E+L T + E G +++ KS + K RR N
Sbjct: 261 SREKLNERLETWRRALETHGFRLSRSKSEYMECKFNKRRRVSN 389
>BI972659
Length = 453
Score = 40.4 bits (93), Expect = 8e-04
Identities = 32/111 (28%), Positives = 54/111 (47%), Gaps = 4/111 (3%)
Frame = +1
Query: 147 QASKEQLQLVNNTLKEFCEASGMKVNLDKSRMLFSKAVDHRRQINLSHIAGI---SRASD 203
+AS + + ++ + L+ F SG+++N KS+ Q N +H A R D
Sbjct: 7 EASWDNIIVLKSMLRGFEMVSGLRINYAKSQFGIVGF-----QPNWAHDAAQLLNCRQLD 171
Query: 204 LG-KYLGIPLLKGRVTKAHFASLIGKMSSRLTSWKNNLLNRAGRVCLAKSV 253
+ YLG+P+ ++ + LI K ++L+ W L+ GRV L KSV
Sbjct: 172 IPFHYLGMPIAVKASSRMVWEPLINKFKAKLSKWNQKYLSMGGRVTLIKSV 324
>BI471325
Length = 421
Score = 39.7 bits (91), Expect = 0.001
Identities = 19/50 (38%), Positives = 29/50 (58%)
Frame = +2
Query: 133 ISHLFFADDVLLFCQASKEQLQLVNNTLKEFCEASGMKVNLDKSRMLFSK 182
+SHL D LFC+ ++ ++ N L F +SG+ VNL KS + FS+
Sbjct: 44 LSHLLSVDFYFLFCRTIDKEADVLKNILTTFEASSGLVVNLRKSEICFSR 193
>TC212055
Length = 776
Score = 36.2 bits (82), Expect = 0.015
Identities = 18/77 (23%), Positives = 42/77 (54%)
Frame = +1
Query: 100 LFVLCMERLAIQIQNLVTQGTWKPVRITKDGVGISHLFFADDVLLFCQASKEQLQLVNNT 159
LF + E L ++ + + + + + K+ + ++ L +ADD + +A+ + + + +
Sbjct: 1 LFNIVAEGLTGLMREALDKSLYSSLMVGKNKIPVNILQYADDTIFLGEATMQNVMTIKSM 180
Query: 160 LKEFCEASGMKVNLDKS 176
L+ F ASG+K++ KS
Sbjct: 181 LRVFELASGLKIHFAKS 231
>TC234877
Length = 534
Score = 35.0 bits (79), Expect = 0.033
Identities = 20/60 (33%), Positives = 30/60 (49%)
Frame = +1
Query: 194 HIAGISRASDLGKYLGIPLLKGRVTKAHFASLIGKMSSRLTSWKNNLLNRAGRVCLAKSV 253
+I G S S YL +PL + + H + ++ + L SWK NLL+ G V L S+
Sbjct: 10 NILGFSVGSLPFIYLSVPLFQEKPRPVHLRPIADRIKAELQSWKGNLLSFMGCVQLIISI 189
>BI470652 similar to PIR|T17102|T171 RNA-directed DNA polymerase (EC
2.7.7.49) (clone AmLi2) - garden snapdragon (fragment),
partial (22%)
Length = 111
Score = 32.7 bits (73), Expect = 0.16
Identities = 15/28 (53%), Positives = 17/28 (60%)
Frame = +1
Query: 72 LSILWNCSKLPSFAPQRGLRQGDPLSPY 99
+SIL N S F P RGLRQG P P+
Sbjct: 25 ISILVNGSPTEEFKPXRGLRQGGPFGPF 108
>AI496436
Length = 414
Score = 32.3 bits (72), Expect = 0.21
Identities = 28/94 (29%), Positives = 43/94 (44%)
Frame = +1
Query: 160 LKEFCEASGMKVNLDKSRMLFSKAVDHRRQINLSHIAGISRASDLGKYLGIPLLKGRVTK 219
L+ F +SG+K+N KS+ D R+ ++ S S YLGIP+
Sbjct: 7 LRCFELSSGLKINFAKSQFGTICKSDIWRKEAADYLNS-SVLSMPFVYLGIPIGANSRHS 183
Query: 220 AHFASLIGKMSSRLTSWKNNLLNRAGRVCLAKSV 253
+ ++ K +L SWK ++ GRV L SV
Sbjct: 184 DVWEPVVRKCERKLASWKQKYVSFGGRVTLINSV 285
>TC215144 similar to UP|ATPQ_ARATH (Q9FT52) ATP synthase D chain,
mitochondrial , complete
Length = 843
Score = 28.1 bits (61), Expect = 4.0
Identities = 14/35 (40%), Positives = 21/35 (60%)
Frame = -3
Query: 176 SRMLFSKAVDHRRQINLSHIAGISRASDLGKYLGI 210
S + FSK D R + + ++ I RAS+LG Y G+
Sbjct: 457 SAISFSKRSDSFRDFSSASLSSIKRASNLGLY*GV 353
>AW101881
Length = 251
Score = 28.1 bits (61), Expect = 4.0
Identities = 14/41 (34%), Positives = 22/41 (53%)
Frame = -1
Query: 187 RRQINLSHIAGISRASDLGKYLGIPLLKGRVTKAHFASLIG 227
RR +S++ G+ ++ YLGIP+ KG K F + G
Sbjct: 179 RRIQEISNLLGLKVSNAPFDYLGIPIFKGNPRKISFKAYCG 57
>TC215146 similar to UP|ATPQ_ARATH (Q9FT52) ATP synthase D chain,
mitochondrial , partial (79%)
Length = 670
Score = 28.1 bits (61), Expect = 4.0
Identities = 14/35 (40%), Positives = 21/35 (60%)
Frame = -1
Query: 176 SRMLFSKAVDHRRQINLSHIAGISRASDLGKYLGI 210
S + FSK D R + + ++ I RAS+LG Y G+
Sbjct: 556 SAISFSKRSDSFRDFSSASLSSIKRASNLGLY*GV 452
>TC229805 similar to PIR|H86466|H86466 protein F23M19.7 [imported] -
Arabidopsis thaliana {Arabidopsis thaliana;} , partial
(43%)
Length = 1039
Score = 27.3 bits (59), Expect = 6.8
Identities = 13/31 (41%), Positives = 18/31 (57%)
Frame = -2
Query: 120 TWKPVRITKDGVGISHLFFADDVLLFCQASK 150
TW + I KDG+ +SH+ LLF +A K
Sbjct: 621 TWMAIIIDKDGLYLSHILAEPVFLLFGKALK 529
>BF070779
Length = 382
Score = 26.9 bits (58), Expect = 8.9
Identities = 8/17 (47%), Positives = 12/17 (70%)
Frame = -3
Query: 53 FGFPPRIVDLIMWGVKC 69
F PPRI +++ W V+C
Sbjct: 143 FRIPPRIAEIVQWWVRC 93
>TC231912 similar to UP|Q93Y40 (Q93Y40) Oxysterol-binding protein, partial
(30%)
Length = 513
Score = 26.9 bits (58), Expect = 8.9
Identities = 13/34 (38%), Positives = 21/34 (61%)
Frame = -3
Query: 176 SRMLFSKAVDHRRQINLSHIAGISRASDLGKYLG 209
S++LFS R I+ ++++G SR SD +LG
Sbjct: 184 SKLLFSAPDFDRSPISSAYLSGRSRESDANNFLG 83
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.325 0.139 0.424
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,815,833
Number of Sequences: 63676
Number of extensions: 168048
Number of successful extensions: 977
Number of sequences better than 10.0: 40
Number of HSP's better than 10.0 without gapping: 974
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 974
length of query: 253
length of database: 12,639,632
effective HSP length: 95
effective length of query: 158
effective length of database: 6,590,412
effective search space: 1041285096
effective search space used: 1041285096
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 58 (26.9 bits)
Lotus: description of TM0381.3