BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0371b.6
(264 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC204535 189 1e-48
TC218359 154 3e-45
TC204540 160 1e-41
BF596737 75 2e-14
TC218358 71 6e-13
TC204536 homologue to UP|Q93WS1 (Q93WS1) Selenium binding protei... 38 0.004
TC204537 38 0.005
TC211041 35 0.026
TC224265 similar to GB|AAQ20909.1|33519188|AY341850 WRKY9 {Oryza... 32 0.38
TC214735 similar to UP|TP14_ARATH (Q8LCA1) Thylakoid membrane ph... 32 0.38
TC213605 weakly similar to UP|RN12_HUMAN (Q9NVW2) RING finger pr... 29 2.5
TC214743 similar to UP|TP14_ARATH (Q8LCA1) Thylakoid membrane ph... 28 5.5
TC204237 similar to UP|Q9SST6 (Q9SST6) Elicitor-induced DNA-bind... 28 5.5
TC224342 28 5.5
TC221860 27 7.2
TC232770 homologue to UP|Q6QUQ3 (Q6QUQ3) Auxin and ethylene resp... 27 7.2
TC222227 similar to UP|Q9ZP54 (Q9ZP54) Poly(ADP-ribose) polymera... 27 9.4
>TC204535
Length = 687
Score = 189 bits (480), Expect = 1e-48
Identities = 96/141 (68%), Positives = 112/141 (79%), Gaps = 6/141 (4%)
Frame = +2
Query: 130 PLDA-GALKFEDKGEAMEVMVSPRRKVENEMATMDCDREEV-----DPTDGFNFWKWSLT 183
P+DA G+LKFEDKGEAME++ SPRR+V + CD+EE D GFN WKWSLT
Sbjct: 53 PMDAAGSLKFEDKGEAMEIITSPRRRVIEKDV---CDKEEESTWEEDNNSGFNLWKWSLT 223
Query: 184 GVGAICSFGVAAATICVLVFGSQQKNKFHQNHKIQFQIYTDDKRIKQVVQQATKLNEAIA 243
G AICSFGVAAATICVL FGSQQ+NK Q+ KI+FQIYTDDKRIKQVVQ ATKLN+AI+
Sbjct: 224 GXXAICSFGVAAATICVLFFGSQQRNKLKQDQKIRFQIYTDDKRIKQVVQHATKLNDAIS 403
Query: 244 ATRGIPLSRAHITYGGYYEAL 264
A RG+P+SRAHI+ GGYY+ L
Sbjct: 404 AARGVPMSRAHISVGGYYDGL 466
>TC218359
Length = 1016
Score = 154 bits (388), Expect(2) = 3e-45
Identities = 77/104 (74%), Positives = 85/104 (81%), Gaps = 1/104 (0%)
Frame = +3
Query: 162 MDCDREEVDPTDGFNFWKWSLTGVGAICSFGVAAATICVLVFGSQQK-NKFHQNHKIQFQ 220
MDC +EE D + GFNFWKWSL GVGAICSFG AAATICVL+ GSQQ+ NK Q+ KI FQ
Sbjct: 234 MDCGKEEEDSSGGFNFWKWSLNGVGAICSFGFAAATICVLILGSQQRNNKIQQDQKIWFQ 413
Query: 221 IYTDDKRIKQVVQQATKLNEAIAATRGIPLSRAHITYGGYYEAL 264
IYTDDKRIKQVV ATKLNEA+AA G+PLSRA IT GGYY+ L
Sbjct: 414 IYTDDKRIKQVV-HATKLNEAMAAVSGVPLSRARITCGGYYDGL 542
Score = 45.4 bits (106), Expect(2) = 3e-45
Identities = 36/81 (44%), Positives = 49/81 (60%), Gaps = 4/81 (4%)
Frame = +1
Query: 80 TTSEKIKAVVVEADEQETLSVSQFFLQLENKFADMKMDSPKSSPR-VLFPPPLDAGALKF 138
TT E IK ++EAD +V+ F +++ +++SPKS R +LFP D GALKF
Sbjct: 7 TTIENIKGGILEADYD---TVAHLFSKIK------EIESPKSIDRGLLFP---DIGALKF 150
Query: 139 EDKG--EAME-VMVSPRRKVE 156
EDKG +A E +M SPR K E
Sbjct: 151 EDKGGDQAQEIIMASPRMKSE 213
>TC204540
Length = 946
Score = 160 bits (404), Expect(2) = 1e-41
Identities = 114/247 (46%), Positives = 137/247 (55%), Gaps = 23/247 (9%)
Frame = +1
Query: 5 NDWVLLSDDGSLDGGDENQIFLGKSNPESKSD----FDMNYFYCTSPKSRDSSTTQLLHD 60
++W LSDDG LD +E K N K D FDM+YF + P R TQL+
Sbjct: 205 DEWEFLSDDGYLDFNEEGGKQKQKQNSFGKLDSEGIFDMDYFCSSPPPPR--VRTQLV-P 375
Query: 61 DPIQFVENT----EDDHVGDNSD---------TTSEKIKAV-VVEADEQETLSVSQFFLQ 106
PIQ ED V D T SEK K VEAD + F ++
Sbjct: 376 LPIQLEPKIGKAPEDILVKDIIKSSPMEVAIATPSEKTKEFETVEADRVKVF----FKIK 543
Query: 107 LENKFADMKMDSPKSSPRVLFPPPLDAGALKFEDKGEAMEVMVSPRRKVENEMATMDCDR 166
+++F DMK R + P AG+LKFEDKGEAME++ SPRR+V + CD+
Sbjct: 544 EKSEFVDMK--------RGILAPMDAAGSLKFEDKGEAMEIITSPRRRVIEKDV---CDK 690
Query: 167 EEV-----DPTDGFNFWKWSLTGVGAICSFGVAAATICVLVFGSQQKNKFHQNHKIQFQI 221
EE D GFN WKWSLTGVGAICSFGVAAATICVL FGSQQ+NK Q+ KI+FQI
Sbjct: 691 EEESTWEEDNNSGFNLWKWSLTGVGAICSFGVAAATICVLFFGSQQRNKLKQDQKIRFQI 870
Query: 222 YTDDKRI 228
YTDDK +
Sbjct: 871 YTDDKSL 891
Score = 27.3 bits (59), Expect(2) = 1e-41
Identities = 12/15 (80%), Positives = 14/15 (93%)
Frame = +2
Query: 226 KRIKQVVQQATKLNE 240
+RIKQVVQ ATKLN+
Sbjct: 902 QRIKQVVQHATKLND 946
>BF596737
Length = 387
Score = 75.5 bits (184), Expect = 2e-14
Identities = 42/81 (51%), Positives = 52/81 (63%), Gaps = 5/81 (6%)
Frame = +3
Query: 135 ALKFEDKGEAMEVMVSPRRKVENEMATMDCDREEV-----DPTDGFNFWKWSLTGVGAIC 189
ALKFEDKGEAME++ SPRRKV + CD+EE + GFN WKWSLTGVGAIC
Sbjct: 3 ALKFEDKGEAMEIITSPRRKVIEKDV---CDKEEETTWEEENNSGFNLWKWSLTGVGAIC 173
Query: 190 SFGVAAATICVLVFGSQQKNK 210
S V + + VF S + ++
Sbjct: 174 SLVV---VLLLFVFSSLEASR 227
Score = 57.0 bits (136), Expect = 8e-09
Identities = 26/36 (72%), Positives = 30/36 (83%)
Frame = +2
Query: 191 FGVAAATICVLVFGSQQKNKFHQNHKIQFQIYTDDK 226
FG AATICVL FGSQQ+NK ++ KI+FQIYTDDK
Sbjct: 176 FGGGAATICVLFFGSQQRNKLKKDQKIRFQIYTDDK 283
>TC218358
Length = 439
Score = 70.9 bits (172), Expect = 6e-13
Identities = 36/46 (78%), Positives = 39/46 (84%)
Frame = +3
Query: 219 FQIYTDDKRIKQVVQQATKLNEAIAATRGIPLSRAHITYGGYYEAL 264
FQIYTDDKRIKQVV ATKLNEA+AA G+PLSRA IT GGYY+ L
Sbjct: 3 FQIYTDDKRIKQVVH-ATKLNEAMAAASGVPLSRARITCGGYYDGL 137
>TC204536 homologue to UP|Q93WS1 (Q93WS1) Selenium binding protein, partial
(6%)
Length = 448
Score = 38.1 bits (87), Expect = 0.004
Identities = 23/58 (39%), Positives = 31/58 (52%), Gaps = 4/58 (6%)
Frame = +3
Query: 5 NDWVLLSDDGSLD----GGDENQIFLGKSNPESKSDFDMNYFYCTSPKSRDSSTTQLL 58
++W LSDDG LD GG + Q +SK FDM+YF C+SP R Q++
Sbjct: 213 DEWEFLSDDGYLDFNEEGGKQKQKQNSFGKLDSKGVFDMDYF-CSSPPPRVRGGPQMI 383
>TC204537
Length = 435
Score = 37.7 bits (86), Expect = 0.005
Identities = 22/50 (44%), Positives = 28/50 (56%), Gaps = 4/50 (8%)
Frame = +1
Query: 5 NDWVLLSDDGSLD----GGDENQIFLGKSNPESKSDFDMNYFYCTSPKSR 50
++W LSDDG LD GG + Q +SK FDM+YF C+SP R
Sbjct: 187 DEWEFLSDDGYLDFNEEGGKQKQKQSSFGKLDSKGVFDMDYF-CSSPPPR 333
>TC211041
Length = 1090
Score = 35.4 bits (80), Expect = 0.026
Identities = 23/80 (28%), Positives = 40/80 (49%), Gaps = 6/80 (7%)
Frame = +2
Query: 21 ENQIFLGKSNPESKS-----DFDMNYFYCTSPKSR-DSSTTQLLHDDPIQFVENTEDDHV 74
+N +FL ++++ + DMNY TS K + SS Q + D + + +++HV
Sbjct: 428 KNWLFLAAQEQDTRNISEEEEPDMNYQERTSQKRKYPSSEEQFVQIDAVGSLVKVKEEHV 607
Query: 75 GDNSDTTSEKIKAVVVEADE 94
GDN+ E I + + DE
Sbjct: 608 GDNNGNIKEAIVIDISDDDE 667
>TC224265 similar to GB|AAQ20909.1|33519188|AY341850 WRKY9 {Oryza sativa
(japonica cultivar-group);} , partial (10%)
Length = 757
Score = 31.6 bits (70), Expect = 0.38
Identities = 27/86 (31%), Positives = 34/86 (39%), Gaps = 2/86 (2%)
Frame = +3
Query: 28 KSNPESKSDFDMNYFYCTSPKSRDSSTTQLLHDDPIQFVENTEDDHVGDNSDTTSEKIKA 87
K NP +S YF CT+ + DDP FV E H N + +
Sbjct: 123 KXNPYPRS-----YFRCTNIMCNVRKHVERAIDDPRSFVTTYEGKH---NHEMPLKNTGT 278
Query: 88 VVVEADEQETLSVSQ--FFLQLENKF 111
V E D Q +LS LQL NK+
Sbjct: 279 VASERDSQASLSKDNP*LTLQLVNKY 356
>TC214735 similar to UP|TP14_ARATH (Q8LCA1) Thylakoid membrane phosphoprotein
14 kDa, chloroplast precursor, partial (57%)
Length = 907
Score = 31.6 bits (70), Expect = 0.38
Identities = 16/58 (27%), Positives = 28/58 (47%)
Frame = +1
Query: 74 VGDNSDTTSEKIKAVVVEADEQETLSVSQFFLQLENKFADMKMDSPKSSPRVLFPPPL 131
+G + T K++ + + TLS+S + D K SP+ SP+ + PPP+
Sbjct: 52 LGASESLTRVKVQVAGMASTSSSTLSISS-----SSTLVDAKAPSPRQSPQCVSPPPV 210
>TC213605 weakly similar to UP|RN12_HUMAN (Q9NVW2) RING finger protein 12
(LIM domain interacting RING finger protein) (RING
finger LIM domain-binding protein) (R-LIM) (NY-REN-43
antigen), partial (6%)
Length = 704
Score = 28.9 bits (63), Expect = 2.5
Identities = 20/73 (27%), Positives = 31/73 (42%), Gaps = 5/73 (6%)
Frame = -3
Query: 12 DDGSLDGGDEN-----QIFLGKSNPESKSDFDMNYFYCTSPKSRDSSTTQLLHDDPIQFV 66
DDG +DGGDE+ + L ++P D D +Y DD +
Sbjct: 690 DDGEVDGGDESDNEEIENLLDSTDPTLGPDSDYDY------------------DDLDEVA 565
Query: 67 ENTEDDHVGDNSD 79
+ ++D +GD SD
Sbjct: 564 DEEDEDLIGDVSD 526
>TC214743 similar to UP|TP14_ARATH (Q8LCA1) Thylakoid membrane phosphoprotein
14 kDa, chloroplast precursor, partial (57%)
Length = 751
Score = 27.7 bits (60), Expect = 5.5
Identities = 14/40 (35%), Positives = 20/40 (50%)
Frame = +1
Query: 92 ADEQETLSVSQFFLQLENKFADMKMDSPKSSPRVLFPPPL 131
A TLS+S + D K SP+ SP+ + PPP+
Sbjct: 1 ATSSSTLSISS-----SSTLVDAKAPSPRQSPQCVSPPPV 105
>TC204237 similar to UP|Q9SST6 (Q9SST6) Elicitor-induced DNA-binding protein
homolog, partial (5%)
Length = 477
Score = 27.7 bits (60), Expect = 5.5
Identities = 14/31 (45%), Positives = 17/31 (54%)
Frame = +3
Query: 22 NQIFLGKSNPESKSDFDMNYFYCTSPKSRDS 52
N + K+NP S+S N C SPKS DS
Sbjct: 258 NSLSFLKNNPTSESHDVSNVQVCESPKSEDS 350
>TC224342
Length = 717
Score = 27.7 bits (60), Expect = 5.5
Identities = 15/46 (32%), Positives = 28/46 (60%)
Frame = -3
Query: 77 NSDTTSEKIKAVVVEADEQETLSVSQFFLQLENKFADMKMDSPKSS 122
N+ ++S ++K ++ + Q +S+ FL++ ADM MD+PK S
Sbjct: 154 NAKSSSSEVKYDLIFSPNQ----LSEKFLRVSKLGADMPMDTPKDS 29
>TC221860
Length = 538
Score = 27.3 bits (59), Expect = 7.2
Identities = 13/31 (41%), Positives = 19/31 (60%)
Frame = +2
Query: 50 RDSSTTQLLHDDPIQFVENTEDDHVGDNSDT 80
R +STT L+ D ++FVE T H D+ D+
Sbjct: 125 RVNSTTLLVGYDLVRFVEGTTTCHASDHQDS 217
>TC232770 homologue to UP|Q6QUQ3 (Q6QUQ3) Auxin and ethylene responsive
GH3-like protein, partial (35%)
Length = 744
Score = 27.3 bits (59), Expect = 7.2
Identities = 20/37 (54%), Positives = 22/37 (59%), Gaps = 2/37 (5%)
Frame = +1
Query: 104 FLQLENKFADMKMDSPKSSPRVLFPPPL--DAGALKF 138
FLQL N F M +DS SSP L PP DA AL+F
Sbjct: 82 FLQLINWFLAMAVDSAISSP--LGPPACEKDAKALRF 186
>TC222227 similar to UP|Q9ZP54 (Q9ZP54) Poly(ADP-ribose) polymerase ,
partial (17%)
Length = 789
Score = 26.9 bits (58), Expect = 9.4
Identities = 12/35 (34%), Positives = 17/35 (48%)
Frame = +2
Query: 171 PTDGFNFWKWSLTGVGAICSFGVAAATICVLVFGS 205
P D WK + G+G + V T C++V GS
Sbjct: 509 PNDSIAEWKRKIDGIGGVFHAKVNKDTNCLVVVGS 613
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.315 0.132 0.385
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,839,155
Number of Sequences: 63676
Number of extensions: 140807
Number of successful extensions: 742
Number of sequences better than 10.0: 34
Number of HSP's better than 10.0 without gapping: 731
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 732
length of query: 264
length of database: 12,639,632
effective HSP length: 96
effective length of query: 168
effective length of database: 6,526,736
effective search space: 1096491648
effective search space used: 1096491648
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 58 (26.9 bits)
Lotus: description of TM0371b.6