BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0368c.9
(764 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
CF922488 142 5e-34
NP334778 reverse transcriptase [Glycine max] 137 1e-32
NP595172 polyprotein [Glycine max] 108 9e-24
NP395547 reverse transcriptase [Glycine max] 106 4e-23
TC233837 similar to UP|Q6WAY3 (Q6WAY3) Gag/pol polyprotein, part... 103 4e-22
BG725601 similar to PIR|H86337|H86 protein F5M15.26 [imported] -... 100 3e-21
NP395548 reverse transcriptase [Glycine max] 94 3e-19
TC219643 weakly similar to UP|Q6WAY7 (Q6WAY7) Gag/pol polyprotei... 77 2e-14
BG839293 49 1e-05
BE803782 31 2.3
AW423706 31 2.3
TC218515 30 5.2
>CF922488
Length = 741
Score = 142 bits (358), Expect = 5e-34
Identities = 75/156 (48%), Positives = 96/156 (61%)
Frame = +3
Query: 364 VKKANGK*PMCVDYTDLNKACPKDSYPLPSIDKLVDGASNNELLSLMDAYSGYHQIKMHS 423
V K +GK MCVDY DLN A PKD +PLP I+ LVD ++ S MD +SGY+QIK+
Sbjct: 3 VLKEDGKV*MCVDYRDLN*ASPKDKFPLPHINVLVDNTTSFSQFSFMDGFSGYNQIKIAP 182
Query: 424 SDKDKTAFMTARANYSYQTMPFGLKNTGATYQRLMDRVFADQVGRNMEVYVGDMIVKSVL 483
D +KT F+T + Y+ M FGLKN GATYQR M +F D + + +EVY+ DMIVKS
Sbjct: 183 EDMEKTTFITLWGTFCYKAMSFGLKNVGATYQRAMVALF*DMMHKEIEVYMDDMIVKSRT 362
Query: 484 EANHHEELLEAFGRLRKHNMRLNLKSSLLVYKEESL 519
E H L + F RLRK+ +RLN + K L
Sbjct: 363 EEEHLVNLRKLFRRLRKYRLRLNPAKCMFEVKSRKL 470
>NP334778 reverse transcriptase [Glycine max]
Length = 431
Score = 137 bits (346), Expect = 1e-32
Identities = 67/134 (50%), Positives = 88/134 (65%)
Frame = +3
Query: 373 MCVDYTDLNKACPKDSYPLPSIDKLVDGASNNELLSLMDAYSGYHQIKMHSSDKDKTAFM 432
MCVDY DLN+A PKD++PLP ID L+ ++ L S MD +SGY+QIKM D +KT F+
Sbjct: 6 MCVDYRDLNRASPKDNFPLPHIDILMANMASFALFSFMDGFSGYNQIKMAPEDMEKTTFI 185
Query: 433 TARANYSYQTMPFGLKNTGATYQRLMDRVFADQVGRNMEVYVGDMIVKSVLEANHHEELL 492
T + Y+ M FGLKN GATY R M +F D + + +E YV +MI KS +E H L
Sbjct: 186 TLWGTFCYKVMSFGLKNFGATYHRAMVALFQDMMHKEIEAYVDEMIAKSRMEEEHLVNLQ 365
Query: 493 EAFGRLRKHNMRLN 506
FG+LRK+ +RLN
Sbjct: 366 NLFGQLRKYRLRLN 407
>NP595172 polyprotein [Glycine max]
Length = 4659
Score = 108 bits (270), Expect = 9e-24
Identities = 61/194 (31%), Positives = 102/194 (52%)
Frame = +1
Query: 310 HRLALDPGIKLVTQTRRRMGDEKEKVIQQEVNKLLAAEFI*EIKYPTWLANVVMVKKANG 369
H + L G V R ++ I++ + ++L I P L +++VKK +G
Sbjct: 1759 HAIPLKQGSGPVKVRPYRYPHTQKDQIEKMIQEMLVQGIIQPSNSPFSLP-ILLVKKKDG 1935
Query: 370 K*PMCVDYTDLNKACPKDSYPLPSIDKLVDGASNNELLSLMDAYSGYHQIKMHSSDKDKT 429
C DY LN KDS+P+P++D+L+D + S +D SGYHQI + D++KT
Sbjct: 1936 SWRFCTDYRALNAITVKDSFPMPTVDELLDELHGAQYFSKLDLRSGYHQILVQPEDREKT 2115
Query: 430 AFMTARANYSYQTMPFGLKNTGATYQRLMDRVFADQVGRNMEVYVGDMIVKSVLEANHHE 489
AF T +Y + MPFGL N AT+Q LM+++F + + + V+ D+++ S +H +
Sbjct: 2116 AFRTHHGHYEWLVMPFGLTNAPATFQCLMNKIFQFALRKFVLVFFDDILIYSASWKDHLK 2295
Query: 490 ELLEAFGRLRKHNM 503
L L++H +
Sbjct: 2296 HLESVLQTLKQHQL 2337
>NP395547 reverse transcriptase [Glycine max]
Length = 762
Score = 106 bits (264), Expect = 4e-23
Identities = 64/199 (32%), Positives = 99/199 (49%), Gaps = 18/199 (9%)
Frame = +1
Query: 336 IQQEVNKLLAAEFI*EIKYPTWLANVVMVKKANG------------------K*PMCVDY 377
+++EV KLL A I I +W++ V +V K G + MC+DY
Sbjct: 1 VRKEVFKLLEAGLIYPISDSSWVSPVQVVPKKGGMTVVKNDRNELIPTRRVTRWRMCIDY 180
Query: 378 TDLNKACPKDSYPLPSIDKLVDGASNNELLSLMDAYSGYHQIKMHSSDKDKTAFMTARAN 437
LN+A KD YPLP +D+++ + +D YSGY+QI + D++KTAF +
Sbjct: 181 RKLNEATRKDHYPLPFMDQMLKRLARQSFYRFLDGYSGYNQIAVDPQDQEKTAFTCPFSV 360
Query: 438 YSYQTMPFGLKNTGATYQRLMDRVFADQVGRNMEVYVGDMIVKSVLEANHHEELLEAFGR 497
++Y+ MPFGL N T+QR M +F D V + +EV++ D N L + R
Sbjct: 361 FAYRRMPFGLCNASTTFQRCMMAIFDDMVEKCIEVFMDDFSFFGASFGNCLANLEKVLQR 540
Query: 498 LRKHNMRLNLKSSLLVYKE 516
K N+ LN + + +E
Sbjct: 541 CEKSNLVLNWEKCHFMVQE 597
>TC233837 similar to UP|Q6WAY3 (Q6WAY3) Gag/pol polyprotein, partial (6%)
Length = 402
Score = 103 bits (256), Expect = 4e-22
Identities = 51/99 (51%), Positives = 66/99 (66%)
Frame = +2
Query: 408 SLMDAYSGYHQIKMHSSDKDKTAFMTARANYSYQTMPFGLKNTGATYQRLMDRVFADQVG 467
S MD +SGY+QI M D +KT F+T +SY+ M FGLKNTGATYQR M +F D +
Sbjct: 5 SFMDGFSGYNQI*MAREDVEKTTFVTLWGTFSYRVMAFGLKNTGATYQRAMVALFHDMMH 184
Query: 468 RNMEVYVGDMIVKSVLEANHHEELLEAFGRLRKHNMRLN 506
+ +EVYV DMI KS E H L + FGRL+K+ ++LN
Sbjct: 185 KEIEVYVDDMIAKSRTETEHLVNLCKLFGRLQKYQLKLN 301
>BG725601 similar to PIR|H86337|H86 protein F5M15.26 [imported] - Arabidopsis
thaliana, partial (1%)
Length = 285
Score = 100 bits (248), Expect = 3e-21
Identities = 50/91 (54%), Positives = 66/91 (71%)
Frame = -3
Query: 310 HRLALDPGIKLVTQTRRRMGDEKEKVIQQEVNKLLAAEFI*EIKYPTWLANVVMVKKANG 369
H+LA+ +KLVTQ +R++ +E+ + + QEV KL A FI +I Y T L +VVMVKK NG
Sbjct: 277 HKLAICNDVKLVTQRKRKIREERCQTV*QEVVKLAIASFIRDINYST*LFSVVMVKKPNG 98
Query: 370 K*PMCVDYTDLNKACPKDSYPLPSIDKLVDG 400
K +C DY DLN ACPKD+YPLP+ID + DG
Sbjct: 97 KWRICTDYIDLN*ACPKDAYPLPNIDHMTDG 5
>NP395548 reverse transcriptase [Glycine max]
Length = 762
Score = 93.6 bits (231), Expect = 3e-19
Identities = 52/162 (32%), Positives = 86/162 (52%), Gaps = 18/162 (11%)
Frame = +1
Query: 336 IQQEVNKLLAAEFI*EIKYPTWLANVVMVKKANG------------------K*PMCVDY 377
+++EV KLL I I W++ V++V K G +C+DY
Sbjct: 1 VRKEVLKLLEVGLIYPISDSAWVSPVLVVSKKEGMTVIRNEKNDLIPTRTVTSWKLCIDY 180
Query: 378 TDLNKACPKDSYPLPSIDKLVDGASNNELLSLMDAYSGYHQIKMHSSDKDKTAFMTARAN 437
LN+A KD +PLP +D++++ + + +DAY GY+QI + D++K AF
Sbjct: 181 RKLNEATRKDHFPLPFMDQMLERLAGHAYYCFLDAYFGYNQIVVDPKDQEKMAFTCPFGV 360
Query: 438 YSYQTMPFGLKNTGATYQRLMDRVFADQVGRNMEVYVGDMIV 479
++Y+ +PFGL N T+Q M +FAD V +++EV++ D V
Sbjct: 361 FAYRRIPFGLCNAPTTFQMCMLAIFADIVEKSIEVFMDDFSV 486
>TC219643 weakly similar to UP|Q6WAY7 (Q6WAY7) Gag/pol polyprotein (Fragment),
partial (8%)
Length = 1320
Score = 77.4 bits (189), Expect = 2e-14
Identities = 40/101 (39%), Positives = 59/101 (57%)
Frame = +3
Query: 264 GDKVLKIGTRLTESQEQRLSKLLEENLDLFAWSHKDMSGIDPNFICHRLALDPGIKLVTQ 323
G + +KIGT +T + L LL + D+FAWS++DM G+ + + HRL L+P V Q
Sbjct: 1014 GKREVKIGTGITAPIREELIILLRDYQDIFAWSYQDMPGLSSDIVQHRLPLNPECSPVKQ 1193
Query: 324 TRRRMGDEKEKVIQQEVNKLLAAEFI*EIKYPTWLANVVMV 364
RRM E I++EV K A F+ +YP W+AN+V +
Sbjct: 1194 KLRRMKPETSLKIKEEVKK*FDAGFLAVARYPKWVANIVPI 1316
>BG839293
Length = 781
Score = 48.5 bits (114), Expect = 1e-05
Identities = 23/60 (38%), Positives = 37/60 (61%)
Frame = +1
Query: 264 GDKVLKIGTRLTESQEQRLSKLLEENLDLFAWSHKDMSGIDPNFICHRLALDPGIKLVTQ 323
G + +KIGT +T + L LL++ D+FAWS++DM G+ + + H+L L+P V Q
Sbjct: 550 GKREVKIGTGITAPIREELIILLKDYQDIFAWSYQDMPGLSSDIVQHQLPLNPECSPVKQ 729
>BE803782
Length = 416
Score = 30.8 bits (68), Expect = 2.3
Identities = 21/47 (44%), Positives = 28/47 (58%), Gaps = 3/47 (6%)
Frame = +2
Query: 558 SRPFPDSYLTRATSQLRFLSALGKMRLSSGVQNAR---RLSVTSRNC 601
S P S LTR +++LR ++ + L S V AR RLSVTSR+C
Sbjct: 23 SSPLRQSLLTRISAELRCAQSM--LPLHSAVAAARMTSRLSVTSRSC 157
>AW423706
Length = 323
Score = 30.8 bits (68), Expect = 2.3
Identities = 21/47 (44%), Positives = 28/47 (58%), Gaps = 3/47 (6%)
Frame = +2
Query: 558 SRPFPDSYLTRATSQLRFLSALGKMRLSSGVQNAR---RLSVTSRNC 601
S P S LTR +++LR ++ + L S V AR RLSVTSR+C
Sbjct: 17 SSPLRQSLLTRISAELRCAQSM--LPLHSAVAAARMTSRLSVTSRSC 151
>TC218515
Length = 606
Score = 29.6 bits (65), Expect = 5.2
Identities = 11/15 (73%), Positives = 12/15 (79%)
Frame = +3
Query: 437 NYSYQTMPFGLKNTG 451
NY Y+ MPFGLKN G
Sbjct: 327 NYCYKVMPFGLKNAG 371
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.337 0.145 0.453
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 29,387,829
Number of Sequences: 63676
Number of extensions: 361991
Number of successful extensions: 2596
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 2533
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2593
length of query: 764
length of database: 12,639,632
effective HSP length: 105
effective length of query: 659
effective length of database: 5,953,652
effective search space: 3923456668
effective search space used: 3923456668
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.7 bits)
S2: 63 (28.9 bits)
Lotus: description of TM0368c.9