BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0342b.11
(137 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC228328 weakly similar to UP|Q6ZFI7 (Q6ZFI7) Calcium-binding EF... 224 8e-60
TC228329 weakly similar to UP|Q6ZFI7 (Q6ZFI7) Calcium-binding EF... 223 2e-59
TC218697 weakly similar to UP|Q6ZFI7 (Q6ZFI7) Calcium-binding EF... 205 6e-54
TC218696 weakly similar to UP|Q6ZFI7 (Q6ZFI7) Calcium-binding EF... 197 1e-51
TC205104 weakly similar to UP|Q6ZFI7 (Q6ZFI7) Calcium-binding EF... 129 6e-31
TC205105 weakly similar to UP|SP4_HUMAN (Q02446) Transcription f... 128 1e-30
TC206503 similar to UP|O82062 (O82062) 39 kDa EF-Hand containing... 52 7e-08
AW756101 52 1e-07
BE608685 44 2e-05
TC227523 weakly similar to UP|Q7Z2B8 (Q7Z2B8) MyoD light chain, ... 33 0.042
TC211131 similar to UP|Q6H739 (Q6H739) Calcium-binding EF hand-l... 32 0.095
BM892093 32 0.095
TC228834 31 0.21
TC211152 UP|Q84P28 (Q84P28) Seed calcium dependent protein kinas... 31 0.21
CK606258 29 0.80
TC222607 weakly similar to UP|POC7_PHLPR (O82040) Polcalcin Phl ... 28 1.4
TC221061 similar to UP|NCS2_CAEEL (P36609) Neuronal calcium sens... 28 1.8
TC212917 weakly similar to UP|Q9LX27 (Q9LX27) Calmodulin-like pr... 28 1.8
TC233913 weakly similar to UP|O22429 (O22429) Calcium binding pr... 28 1.8
BI471390 similar to GP|28416563|gb At1g18890 {Arabidopsis thalia... 28 1.8
>TC228328 weakly similar to UP|Q6ZFI7 (Q6ZFI7) Calcium-binding EF-hand family
protein-like, partial (30%)
Length = 693
Score = 224 bits (572), Expect = 8e-60
Identities = 111/137 (81%), Positives = 124/137 (90%)
Frame = +3
Query: 1 MSVEILDGATIVNFLEDEEAFNISVCNRFALLDTNKDGLLSYEEMLKELQCLRVLETHFG 60
MSVEILDGATIVNFLEDEEA ++SV NRF LDT+ DGLLSY EMLKELQ LRVLETHFG
Sbjct: 42 MSVEILDGATIVNFLEDEEALSVSVLNRFTHLDTDNDGLLSYAEMLKELQSLRVLETHFG 221
Query: 61 IDVKPDPDELGRVYKSLFIQFDHNLNGSVDLEEFKMETREMMLAMADGMGFLPVQMALEE 120
IDV+PDPDEL RVY++LF+QFDHNLNG++DLEEF ET++MMLAMADG+GFLPVQM LEE
Sbjct: 222 IDVEPDPDELARVYEALFLQFDHNLNGTIDLEEFNKETKQMMLAMADGLGFLPVQMVLEE 401
Query: 121 DSILKRAVERECNKVVA 137
DSILK+AVERE NKV A
Sbjct: 402 DSILKKAVERESNKVSA 452
>TC228329 weakly similar to UP|Q6ZFI7 (Q6ZFI7) Calcium-binding EF-hand family
protein-like, partial (80%)
Length = 748
Score = 223 bits (568), Expect = 2e-59
Identities = 110/137 (80%), Positives = 125/137 (90%)
Frame = +2
Query: 1 MSVEILDGATIVNFLEDEEAFNISVCNRFALLDTNKDGLLSYEEMLKELQCLRVLETHFG 60
MSVEILDGATIVNFL+DEEAF+ SV NRFA LDT+ DGLLSY EMLKELQ LRVLETHFG
Sbjct: 122 MSVEILDGATIVNFLQDEEAFSASVLNRFAYLDTDNDGLLSYAEMLKELQSLRVLETHFG 301
Query: 61 IDVKPDPDELGRVYKSLFIQFDHNLNGSVDLEEFKMETREMMLAMADGMGFLPVQMALEE 120
IDV+PDPDEL RVY+SLF+QFDHNLNG++DL+EFK ET++MMLAMA+G+GFLPVQM LEE
Sbjct: 302 IDVEPDPDELARVYESLFVQFDHNLNGTIDLDEFKKETKQMMLAMANGLGFLPVQMVLEE 481
Query: 121 DSILKRAVERECNKVVA 137
DSILK+AVERE KV +
Sbjct: 482 DSILKKAVEREFPKVAS 532
>TC218697 weakly similar to UP|Q6ZFI7 (Q6ZFI7) Calcium-binding EF-hand family
protein-like, partial (38%)
Length = 1058
Score = 205 bits (521), Expect = 6e-54
Identities = 102/138 (73%), Positives = 120/138 (86%), Gaps = 1/138 (0%)
Frame = +1
Query: 1 MSVEILDGATIVNFLEDEEAFNISVCNRFALLDTNKDGLLSYEEMLKELQCLRVLETHFG 60
MSVEILDGATIV F+EDEEAFN+ V + F LDT+KDGLLSY EMLKELQ LRV ETHFG
Sbjct: 64 MSVEILDGATIVGFVEDEEAFNVCVSDLFTQLDTDKDGLLSYAEMLKELQRLRVFETHFG 243
Query: 61 IDVKPDPDELGRVYKSLFIQFDHNLNGSVDLEEFKMETREMMLAMADGMGFLPVQMALEE 120
+DVK DPDEL RVY+S+F+QFDH+LNG VDLEEFK ET+++MLAMA+G+GFLPVQMALE
Sbjct: 244 VDVKRDPDELARVYESMFVQFDHDLNGRVDLEEFKEETKQIMLAMANGLGFLPVQMALEH 423
Query: 121 DSILKRAVERE-CNKVVA 137
DS+L +AV+RE C K+ A
Sbjct: 424 DSLLMKAVQREYCPKIAA 477
>TC218696 weakly similar to UP|Q6ZFI7 (Q6ZFI7) Calcium-binding EF-hand family
protein-like, partial (87%)
Length = 645
Score = 197 bits (502), Expect = 1e-51
Identities = 97/131 (74%), Positives = 115/131 (87%)
Frame = +2
Query: 1 MSVEILDGATIVNFLEDEEAFNISVCNRFALLDTNKDGLLSYEEMLKELQCLRVLETHFG 60
MSVEILDGATIV F+EDEE FN+ V + F+ LDT+KDGLLSY EMLKELQ LRV ETHFG
Sbjct: 74 MSVEILDGATIVGFVEDEEVFNVCVSDLFSQLDTDKDGLLSYAEMLKELQRLRVFETHFG 253
Query: 61 IDVKPDPDELGRVYKSLFIQFDHNLNGSVDLEEFKMETREMMLAMADGMGFLPVQMALEE 120
+DVK DPDEL RVY+SLF+QFDH+LNG VDL+EFK ET+++MLAMA+G+G LPVQMALE
Sbjct: 254 VDVKRDPDELARVYESLFVQFDHDLNGRVDLQEFKEETKQIMLAMANGLGSLPVQMALEH 433
Query: 121 DSILKRAVERE 131
DS+L +AV+RE
Sbjct: 434 DSLLMKAVQRE 466
>TC205104 weakly similar to UP|Q6ZFI7 (Q6ZFI7) Calcium-binding EF-hand family
protein-like, partial (26%)
Length = 745
Score = 129 bits (323), Expect = 6e-31
Identities = 62/136 (45%), Positives = 97/136 (70%), Gaps = 2/136 (1%)
Frame = +3
Query: 1 MSVEILDGATIVNFLEDEEAFNISVCNRFALLDTNKDGLLSYEEMLKELQCLRVLETHFG 60
M V ++DG+T+ +F+ DE AF SV +F +LD N DG+LS E+ + +R++ETHFG
Sbjct: 165 MGVVVIDGSTVRDFVNDEAAFAKSVDEQFGVLDLNNDGVLSRSELRTAFESMRLIETHFG 344
Query: 61 IDVKPDPDELGRVYKSLFIQFDHNLNGSVDLEEFKMETREMMLAMADGMGFLPVQMALEE 120
IDV PD+L ++Y S+F +FD + +G+VD EF+ E R++MLA+ADG+G P++M LE+
Sbjct: 345 IDVATPPDQLAKLYDSIFDKFDGDRSGAVDRREFRDEMRKIMLAIADGLGSFPIRMVLED 524
Query: 121 D--SILKRAVERECNK 134
D S+L++A + E +K
Sbjct: 525 DPNSLLQKAADLEASK 572
>TC205105 weakly similar to UP|SP4_HUMAN (Q02446) Transcription factor Sp4
(SPR-1), partial (3%)
Length = 916
Score = 128 bits (321), Expect = 1e-30
Identities = 61/136 (44%), Positives = 97/136 (70%), Gaps = 2/136 (1%)
Frame = +3
Query: 1 MSVEILDGATIVNFLEDEEAFNISVCNRFALLDTNKDGLLSYEEMLKELQCLRVLETHFG 60
M V ++DG+T+ +F+ DE AF SV +F+ LD N DG+LS E+ + +R++ETHFG
Sbjct: 264 MGVVVIDGSTVRDFVNDETAFTKSVDEQFSALDLNNDGVLSRSELRTAFESMRLIETHFG 443
Query: 61 IDVKPDPDELGRVYKSLFIQFDHNLNGSVDLEEFKMETREMMLAMADGMGFLPVQMALEE 120
IDV P++L ++Y S+F +FD + +G+VD EF+ E R++MLA+ADG+G P++M LE+
Sbjct: 444 IDVSTPPEQLAKLYDSIFDKFDGDRSGAVDRREFRDEMRKIMLAIADGLGSFPIRMVLED 623
Query: 121 D--SILKRAVERECNK 134
D S+L++A + E +K
Sbjct: 624 DPNSLLQKAADLEASK 671
>TC206503 similar to UP|O82062 (O82062) 39 kDa EF-Hand containing protein,
partial (94%)
Length = 1538
Score = 52.4 bits (124), Expect = 7e-08
Identities = 33/114 (28%), Positives = 58/114 (49%), Gaps = 3/114 (2%)
Frame = +3
Query: 4 EILDGATIVNFLEDEEAFNISVCNRFALLDTNKDGLLSYEEM---LKELQCLRVLETHFG 60
++LDG+ I+ + +++ F V ++F LDT+KDG LS +E+ + ++ L H
Sbjct: 180 QVLDGSNIMELVGNQQVFTTFVDHKFHELDTDKDGKLSVKELQPAVADIGAALGLPAH-- 353
Query: 61 IDVKPDPDELGRVYKSLFIQFDHNLNGSVDLEEFKMETREMMLAMADGMGFLPV 114
PD D +Y + +F H +V EFK +++L MA G+ P+
Sbjct: 354 -GTNPDSD---HIYSEVLNEFTHGKQENVSKSEFKEVLSDILLGMAAGLKRDPI 503
>AW756101
Length = 274
Score = 51.6 bits (122), Expect = 1e-07
Identities = 23/74 (31%), Positives = 46/74 (62%)
Frame = +2
Query: 49 LQCLRVLETHFGIDVKPDPDELGRVYKSLFIQFDHNLNGSVDLEEFKMETREMMLAMADG 108
L +R++ETHFG +V +++ ++Y S+ QFD + +G+ F E R++ML + D
Sbjct: 5 LSTMRLMETHFGFNVYKPAEQMAKLYDSILDQFDGDRSGARHRRGFLDEMRKIMLDIVDW 184
Query: 109 MGFLPVQMALEEDS 122
+ + +++ LE+D+
Sbjct: 185 LSTIHIKIVLEDDA 226
>BE608685
Length = 401
Score = 44.3 bits (103), Expect = 2e-05
Identities = 30/106 (28%), Positives = 53/106 (49%), Gaps = 1/106 (0%)
Frame = +3
Query: 4 EILDGATIVNFLEDEEAFNISVCNRFALLDTNKDGLLSYEEMLKELQ-CLRVLETHFGID 62
EI+DG+ I+ + +E+ F+ V ++F LD ++DG LS +KEL+ + + G+
Sbjct: 96 EIVDGSKIMELVGNEKVFSNFVDHKFDELDKDRDGKLS----MKELEPAVADIGAGLGLP 263
Query: 63 VKPDPDELGRVYKSLFIQFDHNLNGSVDLEEFKMETREMMLAMADG 108
+ + +Y + +F H V EFK ++L MA G
Sbjct: 264 AQGTSPDSDHIYFEVLNEFTHGKQEKVSKTEFKEVLSYILLGMAAG 401
>TC227523 weakly similar to UP|Q7Z2B8 (Q7Z2B8) MyoD light chain, partial
(22%)
Length = 1020
Score = 33.1 bits (74), Expect = 0.042
Identities = 22/88 (25%), Positives = 43/88 (48%), Gaps = 13/88 (14%)
Frame = +3
Query: 57 THFGIDVKPDPDELGRVYKSLFIQFDHNLNGSVDLEEFKMETREM-------------ML 103
++ G +++P +E+ K +F +FD N +G + LEE+K R M +
Sbjct: 174 SNVGCNIQPKSEEM----KWVFDKFDTNKDGKITLEEYKAAVRTMGWGIEGTETDESFQV 341
Query: 104 AMADGMGFLPVQMALEEDSILKRAVERE 131
+DG GF+ + ++ ++ +R E E
Sbjct: 342 MDSDGDGFIDFKEFMDMFNVEERVKETE 425
>TC211131 similar to UP|Q6H739 (Q6H739) Calcium-binding EF hand-like protein,
partial (28%)
Length = 484
Score = 32.0 bits (71), Expect = 0.095
Identities = 15/31 (48%), Positives = 20/31 (64%)
Frame = +2
Query: 65 PDPDELGRVYKSLFIQFDHNLNGSVDLEEFK 95
P DE R K++F QFD + NG++D EE K
Sbjct: 380 PKIDESLRKCKAIFEQFDEDSNGAIDQEELK 472
>BM892093
Length = 421
Score = 32.0 bits (71), Expect = 0.095
Identities = 15/38 (39%), Positives = 26/38 (67%)
Frame = +3
Query: 4 EILDGATIVNFLEDEEAFNISVCNRFALLDTNKDGLLS 41
EI+DG+ I+ + +E+ F+ V ++F LD ++DG LS
Sbjct: 306 EIVDGSKIMELVGNEQVFSNFVDHKFDELDKDRDGKLS 419
>TC228834
Length = 1305
Score = 30.8 bits (68), Expect = 0.21
Identities = 18/47 (38%), Positives = 24/47 (50%)
Frame = +2
Query: 3 VEILDGATIVNFLEDEEAFNISVCNRFALLDTNKDGLLSYEEMLKEL 49
V ILDG+T+ EDE+ F + N F LD G +S E+ L
Sbjct: 338 VSILDGSTLRLLSEDEDDFAMLAENLFTDLDVEDKGKISKSEIRNAL 478
>TC211152 UP|Q84P28 (Q84P28) Seed calcium dependent protein kinase b, complete
Length = 1740
Score = 30.8 bits (68), Expect = 0.21
Identities = 26/101 (25%), Positives = 46/101 (44%), Gaps = 15/101 (14%)
Frame = +1
Query: 29 FALLDTNKDGLLSYEEMLKELQCL--RVLETHFGIDVKPDPDELGRVYKSLFIQFDHNLN 86
F ++DT+ G +++EE+ + L+ + ++E+ KSL D + N
Sbjct: 1054 FKMIDTDNSGTITFEELKEGLKSVGSNLMESEI---------------KSLMEAADIDNN 1188
Query: 87 GSVDLEEF--------KMETREMMLAM-----ADGMGFLPV 114
GS+D EF KME E ++A DG G++ +
Sbjct: 1189 GSIDYGEFLAATLHLNKMEREENLVAAFAYFDKDGSGYITI 1311
>CK606258
Length = 627
Score = 28.9 bits (63), Expect = 0.80
Identities = 17/66 (25%), Positives = 33/66 (49%)
Frame = +2
Query: 29 FALLDTNKDGLLSYEEMLKELQCLRVLETHFGIDVKPDPDELGRVYKSLFIQFDHNLNGS 88
F L+D + DG ++ +E+ ++ L + P +E+ + + + D + NGS
Sbjct: 146 FCLIDKDSDGFITVDELATIIRSL---------EGNPTKEEI----QDMISEVDIDGNGS 286
Query: 89 VDLEEF 94
+D EEF
Sbjct: 287 IDFEEF 304
>TC222607 weakly similar to UP|POC7_PHLPR (O82040) Polcalcin Phl p 7
(Calcium-binding pollen allergen Phl p 7) (P7), partial
(32%)
Length = 645
Score = 28.1 bits (61), Expect = 1.4
Identities = 23/66 (34%), Positives = 31/66 (46%)
Frame = +2
Query: 29 FALLDTNKDGLLSYEEMLKELQCLRVLETHFGIDVKPDPDELGRVYKSLFIQFDHNLNGS 88
F L+DTN DG +S E+ + L L G + E + K L D N +G
Sbjct: 476 FKLIDTNGDGKISINELSELLSSL-------GYNKCTAVKEAEGMVKVL----DFNRDGF 622
Query: 89 VDLEEF 94
VDL+EF
Sbjct: 623 VDLDEF 640
>TC221061 similar to UP|NCS2_CAEEL (P36609) Neuronal calcium sensor 2
(NCS-2), partial (9%)
Length = 429
Score = 27.7 bits (60), Expect = 1.8
Identities = 13/33 (39%), Positives = 20/33 (60%)
Frame = +2
Query: 75 KSLFIQFDHNLNGSVDLEEFKMETREMMLAMAD 107
K +F +FD N +G V LEE+K R + A+ +
Sbjct: 182 KWVFQKFDTNKDGKVSLEEYKAAARALDRAIGE 280
>TC212917 weakly similar to UP|Q9LX27 (Q9LX27) Calmodulin-like protein,
partial (49%)
Length = 1086
Score = 27.7 bits (60), Expect = 1.8
Identities = 24/86 (27%), Positives = 38/86 (43%), Gaps = 3/86 (3%)
Frame = +1
Query: 14 FLEDEEAFNISVCNRFALLDTNKDGLLSYEEMLKELQCLRVLETHFGIDVKPDPDELGRV 73
F+ D+E +I V D+N DGL+ +EE CL E G + + +G
Sbjct: 412 FMADKEVDDIVV-----KYDSNSDGLIDFEEF-----CLLTSECVGGDHHEKEGGVMGNE 561
Query: 74 ---YKSLFIQFDHNLNGSVDLEEFKM 96
K F FD + +G + +EE +
Sbjct: 562 EVDLKEAFDVFDKDNDGLISVEELAL 639
>TC233913 weakly similar to UP|O22429 (O22429) Calcium binding protein,
partial (14%)
Length = 511
Score = 27.7 bits (60), Expect = 1.8
Identities = 12/24 (50%), Positives = 16/24 (66%)
Frame = +1
Query: 29 FALLDTNKDGLLSYEEMLKELQCL 52
F L+DTN DG +S E+ + L CL
Sbjct: 433 FKLIDTNGDGKISATELSEVLSCL 504
>BI471390 similar to GP|28416563|gb At1g18890 {Arabidopsis thaliana}, partial
(25%)
Length = 421
Score = 27.7 bits (60), Expect = 1.8
Identities = 10/17 (58%), Positives = 15/17 (87%)
Frame = +3
Query: 29 FALLDTNKDGLLSYEEM 45
F L+DT+KDG ++YEE+
Sbjct: 255 FTLMDTDKDGKVTYEEL 305
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.321 0.139 0.388
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,696,238
Number of Sequences: 63676
Number of extensions: 49763
Number of successful extensions: 218
Number of sequences better than 10.0: 48
Number of HSP's better than 10.0 without gapping: 211
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 215
length of query: 137
length of database: 12,639,632
effective HSP length: 87
effective length of query: 50
effective length of database: 7,099,820
effective search space: 354991000
effective search space used: 354991000
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 54 (25.4 bits)
Lotus: description of TM0342b.11