BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0336.12
(267 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC232864 weakly similar to UP|Q7GAD9 (Q7GAD9) Expressed protein,... 134 6e-32
TC216209 similar to UP|Q7GAD9 (Q7GAD9) Expressed protein, partia... 126 9e-30
TC213249 similar to UP|Q7GAD9 (Q7GAD9) Expressed protein, partia... 111 3e-25
BE555005 72 2e-22
TC223015 similar to UP|Q7GAD9 (Q7GAD9) Expressed protein, partia... 99 2e-21
BM143208 60 1e-17
TC225886 similar to UP|Q9LRE9 (Q9LRE9) Cytosolic aldehyde dehydr... 32 0.39
TC214368 homologue to UP|Q9M4R0 (Q9M4R0) Ubiquitin conjugating p... 30 1.5
TC214108 similar to UP|RK29_ARATH (Q9FJP3) 50S ribosomal protein... 30 1.5
TC204134 homologue to UP|Q93XW1 (Q93XW1) 14-3-3 protein, partial... 29 1.9
BM085598 28 3.3
TC216538 28 3.3
TC219992 similar to UP|Q75M40 (Q75M40) Unknow protein, partial (... 28 3.3
TC211846 28 4.3
TC229161 similar to UP|CAPB_ARATH (Q9M9G7) Probable F-actin capp... 27 7.3
TC217467 similar to UP|Q949B2 (Q949B2) AP2 domain-containing pro... 27 7.3
TC225308 homologue to UP|RS25_LYCES (P46301) 40S ribosomal prote... 27 7.3
TC225309 homologue to UP|R25A_ARATH (Q9SIK2) 40S ribosomal prote... 27 9.6
TC208034 weakly similar to UP|Q9LPJ0 (Q9LPJ0) F6N18.12, partial ... 27 9.6
TC206861 27 9.6
>TC232864 weakly similar to UP|Q7GAD9 (Q7GAD9) Expressed protein, partial
(19%)
Length = 714
Score = 134 bits (336), Expect = 6e-32
Identities = 82/190 (43%), Positives = 108/190 (56%), Gaps = 1/190 (0%)
Frame = +1
Query: 30 PPLGLKRTTSAMKEREKMWRKMQKSKKQNSSTTLKFAPATFYAKLLNVGKYNFIPTTVNQ 89
P LGLK T + +M + + S+ ++ K F +L +G + +
Sbjct: 10 PLLGLKITMTP-----EMLQPTKTSQVAANNKVEKLKAVQFPMDMLRIGYFKIEAKYPYE 174
Query: 90 LVVKCQFARRKLIWEILDSSKNKKFKAEVPWDRISAIRAFIEENKTEILEIELDEQPIFH 149
LV KC +AR+KLIWEIL KFK E+ ISAIRA +EEN ILEIELD+ P F
Sbjct: 175 LVAKCYYARQKLIWEILHDGL--KFKIEIHCQNISAIRAVMEENSPGILEIELDKVPSFF 348
Query: 150 KEINSQ-STHTTWEISDHDFTGGHAMTYRIHHLEFASGDLGQHYENMLKCDERLMKLSQQ 208
+EI+ + HTTW IS HDFT G A YR H+L+F G L QHY +L+ D RL++LS +
Sbjct: 349 REIDPKPKKHTTWTIS-HDFTDGQASEYRRHYLQFPHGVLDQHYIKLLQSDNRLLELSLR 525
Query: 209 PFPRLDSPYF 218
PFP YF
Sbjct: 526 PFPSSHFAYF 555
>TC216209 similar to UP|Q7GAD9 (Q7GAD9) Expressed protein, partial (36%)
Length = 1457
Score = 126 bits (317), Expect = 9e-30
Identities = 77/207 (37%), Positives = 109/207 (52%), Gaps = 17/207 (8%)
Frame = +1
Query: 31 PLGLKRTTSA---------MKEREKMWRKMQKSKKQNS-------STTLKFAPATFYAKL 74
PLGL+ S + ++E+ +K QK+ +S + K + F +
Sbjct: 277 PLGLRLRKSPSLLDLIQMRLSQQEESKKKDQKASSSSSVAAAAAAAADSKLKASNFPGTI 456
Query: 75 LNVGKYNFIPTTVNQLVVKCQFARRKLIWEILDSSKNKKFKAEVPWDRISAIRAFIEENK 134
L +G + + LV KC FA+ KL+WE+LD K K E+PW I AI+A E+
Sbjct: 457 LKIGNWEYKSRYEGDLVAKCYFAKHKLVWEVLDGCL--KNKIEIPWSDIMAIKANYPEDG 630
Query: 135 TEILEIELDEQPIFHKEINSQST-HTTWEISDHDFTGGHAMTYRIHHLEFASGDLGQHYE 193
LE+ L +P+F +EIN Q HT W+ + DFTGG A R H L+ G LG+H+E
Sbjct: 631 PGTLEVVLARRPLFFREINPQPRKHTLWQATS-DFTGGQASINRRHFLQCPQGLLGKHFE 807
Query: 194 NMLKCDERLMKLSQQPFPRLDSPYFGP 220
+++CD RL LSQ P LDSPYF P
Sbjct: 808 KLIQCDPRLNYLSQHPDLVLDSPYFEP 888
>TC213249 similar to UP|Q7GAD9 (Q7GAD9) Expressed protein, partial (40%)
Length = 641
Score = 111 bits (278), Expect = 3e-25
Identities = 59/156 (37%), Positives = 88/156 (55%), Gaps = 1/156 (0%)
Frame = +2
Query: 64 KFAPATFYAKLLNVGKYNFIPTTVNQLVVKCQFARRKLIWEILDSSKNKKFKAEVPWDRI 123
K + F A LL +G + + LV KC FA+ KL+WE+L+ K K E+ W I
Sbjct: 158 KLKASNFPASLLRIGSWEYKSRYEGDLVAKCYFAKHKLVWEVLEGGL--KSKIEIQWSDI 331
Query: 124 SAIRAFIEENKTEILEIELDEQPIFHKEINSQST-HTTWEISDHDFTGGHAMTYRIHHLE 182
A++A ++ L + L QP++ +E N Q HT W+ + DFT G + +R+H L+
Sbjct: 332 MALKAHCPDDGPSTLTVVLARQPLYFRETNPQPRKHTLWQATA-DFTDGQSSKHRLHFLQ 508
Query: 183 FASGDLGQHYENMLKCDERLMKLSQQPFPRLDSPYF 218
G L +H+E +++CD RL LSQQP LDSP+F
Sbjct: 509 CPQGLLAKHFEKLIQCDMRLNFLSQQPEIILDSPHF 616
>BE555005
Length = 431
Score = 72.4 bits (176), Expect(2) = 2e-22
Identities = 39/85 (45%), Positives = 53/85 (61%), Gaps = 1/85 (1%)
Frame = +2
Query: 135 TEILEIELDEQPIFHKEINSQST-HTTWEISDHDFTGGHAMTYRIHHLEFASGDLGQHYE 193
T ++ +L +P+F +EIN Q HT W+ + DFTGG A R H L+ G LG+H+E
Sbjct: 179 TTLMMDQLARRPLFFREINPQPRKHTLWQATS-DFTGGQAGINRRHFLQCPQGLLGKHFE 355
Query: 194 NMLKCDERLMKLSQQPFPRLDSPYF 218
+++CD RL LSQQ LDSPYF
Sbjct: 356 KLIQCDPRLNYLSQQADLVLDSPYF 430
Score = 50.4 bits (119), Expect(2) = 2e-22
Identities = 24/60 (40%), Positives = 34/60 (56%)
Frame = +1
Query: 69 TFYAKLLNVGKYNFIPTTVNQLVVKCQFARRKLIWEILDSSKNKKFKAEVPWDRISAIRA 128
+F +L +G + + LV KC FA+ KL+WE+LD K K E+PW I AI+A
Sbjct: 4 SFPGTILKIGNWEYKSRYEGDLVAKCYFAKHKLVWEVLDGC--LKNKIEIPWSDIMAIKA 177
>TC223015 similar to UP|Q7GAD9 (Q7GAD9) Expressed protein, partial (29%)
Length = 428
Score = 99.0 bits (245), Expect = 2e-21
Identities = 53/140 (37%), Positives = 75/140 (52%), Gaps = 1/140 (0%)
Frame = +2
Query: 64 KFAPATFYAKLLNVGKYNFIPTTVNQLVVKCQFARRKLIWEILDSSKNKKFKAEVPWDRI 123
K + F A LL +G + + LV KC FA+ KL+WE+L+ K K E+ W I
Sbjct: 11 KLKASNFPASLLRIGSWEYKSKHEGDLVAKCYFAKHKLVWEVLEGEL--KNKMEIQWSDI 184
Query: 124 SAIRAFIEENKTEILEIELDEQPIFHKEINSQ-STHTTWEISDHDFTGGHAMTYRIHHLE 182
A++A + L + L QP+F KE N Q HT W+ + DFT G A +R H LE
Sbjct: 185 MALKANCPDTGPSSLTVVLARQPLFFKETNPQPRKHTIWQATS-DFTEGEACKHRQHFLE 361
Query: 183 FASGDLGQHYENMLKCDERL 202
F G L +H+E +++CD L
Sbjct: 362 FPQGLLAKHFEKLIQCDTHL 421
>BM143208
Length = 448
Score = 59.7 bits (143), Expect(2) = 1e-17
Identities = 31/86 (36%), Positives = 47/86 (54%), Gaps = 1/86 (1%)
Frame = +1
Query: 93 KCQFARRKLIWEILDSSKNKKFKAEVPWDRISAIRAFIEENKTEILEIELDEQPIFHKEI 152
KC FA+ K +WE+L+ K K E+ W I A++A +N L + L QP++ +E
Sbjct: 4 KCYFAKHKKVWEVLEGGL--KSKIEIQWSDIMALKAHCPDNGPSTLTVVLARQPLYFRET 177
Query: 153 NSQS-THTTWEISDHDFTGGHAMTYR 177
N Q HT W+ + DFT G + +R
Sbjct: 178 NPQPIKHTLWQ-ATADFTDGQSSKHR 252
Score = 47.0 bits (110), Expect(2) = 1e-17
Identities = 22/45 (48%), Positives = 30/45 (65%)
Frame = +3
Query: 174 MTYRIHHLEFASGDLGQHYENMLKCDERLMKLSQQPFPRLDSPYF 218
M R+H L+ G L +H+E +++CD RL LSQQP LDSP+F
Sbjct: 291 MVCRLHFLQCPQGLLAKHFEKLIQCDMRLNFLSQQPEIILDSPHF 425
>TC225886 similar to UP|Q9LRE9 (Q9LRE9) Cytosolic aldehyde dehydrogenase,
partial (70%)
Length = 1322
Score = 31.6 bits (70), Expect = 0.39
Identities = 16/44 (36%), Positives = 23/44 (51%)
Frame = +3
Query: 217 YFGPPVLFKNIRDYNTVQHDELMEQVMALDRTQPFLSELKSTIN 260
YF P +F NIR+ + DE+ VMAL + + +KS N
Sbjct: 717 YFIEPTIFSNIREDMLIAQDEIFGPVMALKKFKTTEEAIKSANN 848
>TC214368 homologue to UP|Q9M4R0 (Q9M4R0) Ubiquitin conjugating protein,
complete
Length = 793
Score = 29.6 bits (65), Expect = 1.5
Identities = 21/66 (31%), Positives = 30/66 (44%), Gaps = 8/66 (12%)
Frame = -2
Query: 101 LIWEILDSSKNKKFKAEVPWDRISAIRAFIEENKTEILEIELDE------QP--IFHKEI 152
L+W IL KN+ +A +PW I I ++ IL D QP I H+ +
Sbjct: 405 LVWIILSKLKNQLKRASIPWSIIRTKYDSIPQHDVVILRGATDSCRRVLLQPLEISHEPL 226
Query: 153 NSQSTH 158
S+S H
Sbjct: 225 PSRSRH 208
>TC214108 similar to UP|RK29_ARATH (Q9FJP3) 50S ribosomal protein L29,
chloroplast precursor, partial (68%)
Length = 1202
Score = 29.6 bits (65), Expect = 1.5
Identities = 16/45 (35%), Positives = 21/45 (46%)
Frame = +1
Query: 42 KEREKMWRKMQKSKKQNSSTTLKFAPATFYAKLLNVGKYNFIPTT 86
+ERE+ WR + S SS+T+ F P F L IP T
Sbjct: 61 RERERAWRMLSLSVASPSSSTISFLPKPFNGIQLRRSATCSIPPT 195
>TC204134 homologue to UP|Q93XW1 (Q93XW1) 14-3-3 protein, partial (66%)
Length = 1031
Score = 29.3 bits (64), Expect = 1.9
Identities = 20/58 (34%), Positives = 30/58 (51%)
Frame = +2
Query: 3 SKPPFGLELSSSTMNEREPHLRNVCPKPPLGLKRTTSAMKEREKMWRKMQKSKKQNSS 60
SKPPF L LSS +++ PHL P L L S ++ + WR + + K+ S+
Sbjct: 44 SKPPFTLSLSSFSVS---PHLLTFSL*PDLALAL*LSRIR---RQWRPLPRLAKRTST 199
>BM085598
Length = 426
Score = 28.5 bits (62), Expect = 3.3
Identities = 18/61 (29%), Positives = 28/61 (45%), Gaps = 1/61 (1%)
Frame = -3
Query: 25 NVCPKPPLGLKRTT-SAMKEREKMWRKMQKSKKQNSSTTLKFAPATFYAKLLNVGKYNFI 83
N C + P G++ T SAMK R +MW ++ S T+ A+ VG + +
Sbjct: 286 NRCSEEPHGIENTCGSAMKHRIRMWNVHREGFGGGSMRTVNITSLELDAEGFEVGISDSV 107
Query: 84 P 84
P
Sbjct: 106 P 104
>TC216538
Length = 523
Score = 28.5 bits (62), Expect = 3.3
Identities = 9/11 (81%), Positives = 11/11 (99%)
Frame = +3
Query: 161 WEISDHDFTGG 171
WE+SDHDF+GG
Sbjct: 171 WEVSDHDFSGG 203
>TC219992 similar to UP|Q75M40 (Q75M40) Unknow protein, partial (33%)
Length = 937
Score = 28.5 bits (62), Expect = 3.3
Identities = 14/32 (43%), Positives = 22/32 (68%), Gaps = 4/32 (12%)
Frame = +3
Query: 150 KEINSQS-THTTWEIS---DHDFTGGHAMTYR 177
+EI +S THT+W+IS +H+ G H +TY+
Sbjct: 147 EEITRRSLTHTSWDISNSREHERRGWHPVTYK 242
>TC211846
Length = 272
Score = 28.1 bits (61), Expect = 4.3
Identities = 13/31 (41%), Positives = 15/31 (47%)
Frame = +3
Query: 6 PFGLELSSSTMNEREPHLRNVCPKPPLGLKR 36
P G+EL+ E L N P PPL KR
Sbjct: 117 PSGIELAKQRAQEVAARLLNAAPPPPLDAKR 209
>TC229161 similar to UP|CAPB_ARATH (Q9M9G7) Probable F-actin capping
protein beta subunit (CapZ-beta), complete
Length = 1205
Score = 27.3 bits (59), Expect = 7.3
Identities = 14/42 (33%), Positives = 22/42 (52%)
Frame = +3
Query: 42 KEREKMWRKMQKSKKQNSSTTLKFAPATFYAKLLNVGKYNFI 83
+ERE+ WR++ + KK+ S T LK A L + Y +
Sbjct: 9 RERERWWREI-RIKKEESETNLKRVDRNCCASLASTSVYECV 131
>TC217467 similar to UP|Q949B2 (Q949B2) AP2 domain-containing protein Rap211,
partial (48%)
Length = 1166
Score = 27.3 bits (59), Expect = 7.3
Identities = 13/41 (31%), Positives = 18/41 (43%)
Frame = +3
Query: 27 CPKPPLGLKRTTSAMKEREKMWRKMQKSKKQNSSTTLKFAP 67
CP PP RT+ + + W + K+ K N S F P
Sbjct: 432 CPAPPAPPPRTSKLRRLKPPPWISVTKAMKPNPS*AKPFPP 554
>TC225308 homologue to UP|RS25_LYCES (P46301) 40S ribosomal protein S25,
complete
Length = 622
Score = 27.3 bits (59), Expect = 7.3
Identities = 18/74 (24%), Positives = 35/74 (46%), Gaps = 2/74 (2%)
Frame = +2
Query: 30 PPLGLKRTTSAMKEREKMWRKMQKSKKQNSSTTLKFAPATFYAKLLNVGKYNFIPTTV-- 87
PP + K+++K W K ++ +K N+ + F AT+ L KY I ++
Sbjct: 137 PPSSKPAKSGGGKQKKKKWSKGKQKEKVNNQ--VLFDQATYDKLLSEAPKYKLITPSILS 310
Query: 88 NQLVVKCQFARRKL 101
++L + AR+ +
Sbjct: 311 DRLRINGSLARKAI 352
>TC225309 homologue to UP|R25A_ARATH (Q9SIK2) 40S ribosomal protein S25-1,
complete
Length = 589
Score = 26.9 bits (58), Expect = 9.6
Identities = 23/94 (24%), Positives = 43/94 (45%), Gaps = 9/94 (9%)
Frame = +2
Query: 17 NEREPHLRN--VCPK-----PPLGLKRTTSAMKEREKMWRKMQKSKKQNSSTTLKFAPAT 69
+E HLR+ + PK PP + K+++K W K ++ +K N+ + F T
Sbjct: 56 SEGSRHLRSDFMAPKKDKAPPPSSKPAKSGGGKQKKKKWSKGKQKEKVNN--MVLFDQGT 229
Query: 70 FYAKLLNVGKYNFIPTTV--NQLVVKCQFARRKL 101
+ L KY I ++ ++L + AR+ +
Sbjct: 230 YDKLLSEAPKYKLITPSILSDRLRINGSLARKAI 331
>TC208034 weakly similar to UP|Q9LPJ0 (Q9LPJ0) F6N18.12, partial (46%)
Length = 1183
Score = 26.9 bits (58), Expect = 9.6
Identities = 20/66 (30%), Positives = 28/66 (42%)
Frame = -2
Query: 4 KPPFGLELSSSTMNEREPHLRNVCPKPPLGLKRTTSAMKEREKMWRKMQKSKKQNSSTTL 63
KPP L S + +P R + PPL +RT + + R S ++ SS
Sbjct: 780 KPPVSRTLCISEWDPLDPGQRTLTQHPPLRPRRTIALPRHR---------SHRRRSS*PA 628
Query: 64 KFAPAT 69
APAT
Sbjct: 627 AAAPAT 610
>TC206861
Length = 1326
Score = 26.9 bits (58), Expect = 9.6
Identities = 17/64 (26%), Positives = 31/64 (47%), Gaps = 1/64 (1%)
Frame = -1
Query: 50 KMQKSKKQNSSTTLKFAPATFYAKLLNVGK-YNFIPTTVNQLVVKCQFARRKLIWEILDS 108
+ +K +K+N++T + + + + + GK + F + N L C F R KL+W
Sbjct: 879 QFRKEQKRNNNTIFQVSTCQNESIVFHHGKMFGFRVVSFNIL---CSFTRTKLLWNNFHC 709
Query: 109 SKNK 112
K K
Sbjct: 708 HKGK 697
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.318 0.133 0.394
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,807,323
Number of Sequences: 63676
Number of extensions: 156639
Number of successful extensions: 872
Number of sequences better than 10.0: 40
Number of HSP's better than 10.0 without gapping: 865
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 867
length of query: 267
length of database: 12,639,632
effective HSP length: 96
effective length of query: 171
effective length of database: 6,526,736
effective search space: 1116071856
effective search space used: 1116071856
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 58 (26.9 bits)
Lotus: description of TM0336.12