BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0333b.4
(162 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC216434 similar to UP|Q8GT65 (Q8GT65) Serpin-like protein (Frag... 157 2e-39
TC216435 similar to UP|Q8GT65 (Q8GT65) Serpin-like protein (Frag... 154 2e-38
TC203426 homologue to UP|Q72Y61 (Q72Y61) Conserved domain protei... 30 0.50
TC203450 homologue to UP|DAG_ANTMA (Q38732) DAG protein, chlorop... 30 0.66
TC216436 similar to UP|Q8GT65 (Q8GT65) Serpin-like protein (Frag... 28 2.5
TC203810 weakly similar to UP|Q9M686 (Q9M686) Aldehyde dehydroge... 27 3.3
TC207602 homologue to UP|C773_SOYBN (O48928) Cytochrome P450 77A... 27 4.3
TC227021 weakly similar to UP|O65009 (O65009) BURP domain contai... 26 7.3
BG237933 similar to GP|20453162|gb At5g56885 {Arabidopsis thalia... 26 9.5
>TC216434 similar to UP|Q8GT65 (Q8GT65) Serpin-like protein (Fragment),
partial (96%)
Length = 1436
Score = 157 bits (398), Expect = 2e-39
Identities = 88/146 (60%), Positives = 107/146 (73%), Gaps = 1/146 (0%)
Frame = +1
Query: 7 QDGLSPLIGKMASEPGFLEGRLPQHKVKLASFKIPRFDISFAFEASDVLKELGVVLPFSQ 66
+DGL L K+ASE GFLE +LP +K+++ F+IPRF ISF FEAS+VLKELGVVLPFS
Sbjct: 739 KDGLLALAEKLASESGFLERKLPNNKLEVGDFRIPRFKISFGFEASNVLKELGVVLPFSV 918
Query: 67 GDADFTKMVKVNTPFNELYVESIFQKVFIKVHEEGTEAAAATIRALRGGGGP-PQGLKFV 125
G T+MV N L+V IF K FI+V+EEGTEAAAAT ++ G P + FV
Sbjct: 919 G--GLTEMVDSAVGQN-LFVSDIFHKSFIEVNEEGTEAAAATAATIQFGCAMFPTEIDFV 1089
Query: 126 ADHPFLFLIREDFSGTILFVGQVLNP 151
ADHPFLFLIRED +GT+LF+GQVLNP
Sbjct: 1090ADHPFLFLIREDLTGTVLFIGQVLNP 1167
>TC216435 similar to UP|Q8GT65 (Q8GT65) Serpin-like protein (Fragment),
partial (37%)
Length = 820
Score = 154 bits (388), Expect = 2e-38
Identities = 88/149 (59%), Positives = 107/149 (71%), Gaps = 2/149 (1%)
Frame = +2
Query: 8 DGLSPLIGKMASEPGFLEGRLPQHKVKLASFKIPRFDISFAFEASDVLKELGVVLPFSQG 67
DGL L K+ASE GFLE +LP KV++ F+IPRF ISF FE S+VLKELGVVLPFS G
Sbjct: 11 DGLLALAEKLASESGFLERKLPNQKVEVGDFRIPRFKISFGFEVSNVLKELGVVLPFSVG 190
Query: 68 DADFTKMVKVNTPFNE-LYVESIFQKVFIKVHEEGTEAAAATIRALR-GGGGPPQGLKFV 125
T+M V++P + L V +IF K FI+V+EEGTEAAAAT +R P + FV
Sbjct: 191 --GLTEM--VDSPVGQNLCVSNIFHKSFIEVNEEGTEAAAATSATIRLRSAMLPTKIDFV 358
Query: 126 ADHPFLFLIREDFSGTILFVGQVLNPLGG 154
ADHPFLFLIRED +GT+LF+GQVL+P G
Sbjct: 359 ADHPFLFLIREDLTGTVLFIGQVLDPRAG 445
>TC203426 homologue to UP|Q72Y61 (Q72Y61) Conserved domain protein, partial
(90%)
Length = 791
Score = 30.0 bits (66), Expect = 0.50
Identities = 14/45 (31%), Positives = 20/45 (44%)
Frame = -2
Query: 21 PGFLEGRLPQHKVKLASFKIPRFDISFAFEASDVLKELGVVLPFS 65
P F+ +V L F + D +F+F S L + LPFS
Sbjct: 556 PNFVNSSCACSRVNLPDFSCSKIDFAFSFACSSKLANMSPCLPFS 422
>TC203450 homologue to UP|DAG_ANTMA (Q38732) DAG protein, chloroplast
precursor, partial (53%)
Length = 1196
Score = 29.6 bits (65), Expect = 0.66
Identities = 15/35 (42%), Positives = 22/35 (62%), Gaps = 1/35 (2%)
Frame = -2
Query: 92 KVFIKVH-EEGTEAAAATIRALRGGGGPPQGLKFV 125
K+ K H E+ +EAAA+ + LR GGGP L ++
Sbjct: 763 KITCKAHVEDESEAAASCLGLLRSGGGPSLRLSYL 659
>TC216436 similar to UP|Q8GT65 (Q8GT65) Serpin-like protein (Fragment),
partial (40%)
Length = 489
Score = 27.7 bits (60), Expect = 2.5
Identities = 13/23 (56%), Positives = 16/23 (69%)
Frame = +2
Query: 7 QDGLSPLIGKMASEPGFLEGRLP 29
+DGL L K+A + GFLE RLP
Sbjct: 419 KDGLLALAEKLAFDSGFLERRLP 487
>TC203810 weakly similar to UP|Q9M686 (Q9M686) Aldehyde dehydrogenase
(Fragment), partial (58%)
Length = 766
Score = 27.3 bits (59), Expect = 3.3
Identities = 12/27 (44%), Positives = 18/27 (66%)
Frame = +2
Query: 99 EEGTEAAAATIRALRGGGGPPQGLKFV 125
E+ +EAAA+ + LR GGGP L ++
Sbjct: 146 EDESEAAASCLGLLRSGGGPSLRLSYL 226
>TC207602 homologue to UP|C773_SOYBN (O48928) Cytochrome P450 77A3 , partial
(41%)
Length = 845
Score = 26.9 bits (58), Expect = 4.3
Identities = 18/35 (51%), Positives = 21/35 (59%), Gaps = 1/35 (2%)
Frame = +1
Query: 103 EAAAATIRALRGGGGPPQGL-KFVADHPFLFLIRE 136
E+ ATI+ RGGGG L FVA PF FLI +
Sbjct: 601 ESLRATIKP-RGGGGEKVHL*NFVAPPPFWFLISQ 702
>TC227021 weakly similar to UP|O65009 (O65009) BURP domain containing
protein, partial (47%)
Length = 1147
Score = 26.2 bits (56), Expect = 7.3
Identities = 11/29 (37%), Positives = 16/29 (54%)
Frame = +3
Query: 14 IGKMASEPGFLEGRLPQHKVKLASFKIPR 42
+ K P F +G LP H + L SF +P+
Sbjct: 687 VRKYHRNPSFQDGGLPYHALPLYSFLLPQ 773
>BG237933 similar to GP|20453162|gb At5g56885 {Arabidopsis thaliana}, partial
(6%)
Length = 312
Score = 25.8 bits (55), Expect = 9.5
Identities = 12/31 (38%), Positives = 16/31 (50%)
Frame = -3
Query: 103 EAAAATIRALRGGGGPPQGLKFVADHPFLFL 133
EA AAT+ L G P+ +A HP L +
Sbjct: 115 EAMAATLATLSKGTSVPRDWSIIASHPSLLV 23
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.322 0.143 0.415
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,912,877
Number of Sequences: 63676
Number of extensions: 68068
Number of successful extensions: 292
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 286
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 287
length of query: 162
length of database: 12,639,632
effective HSP length: 90
effective length of query: 72
effective length of database: 6,908,792
effective search space: 497433024
effective search space used: 497433024
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 55 (25.8 bits)
Lotus: description of TM0333b.4