BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0327.8
(250 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC229090 similar to UP|Q84ZV0 (Q84ZV0) R 14 protein, partial (31%) 236 1e-62
TC228120 similar to UP|Q84ZV0 (Q84ZV0) R 14 protein, partial (31%) 231 2e-61
NP595166 R 14 protein [Glycine max] 223 6e-59
NP595167 R 13 protein [Glycine max] 202 2e-52
TC228121 similar to UP|Q84ZV0 (Q84ZV0) R 14 protein, partial (9%) 94 8e-20
TC226225 31 0.46
TC226224 30 1.0
TC211281 30 1.4
TC223326 29 2.3
TC204617 similar to UP|Q9ASZ9 (Q9ASZ9) At1g60730/F8A5_24, partia... 28 3.9
TC208458 28 5.1
BM523240 28 5.1
TC214245 similar to UP|Q944G1 (Q944G1) Phosphomevalonate kinase,... 27 6.7
TC205836 27 8.8
>TC229090 similar to UP|Q84ZV0 (Q84ZV0) R 14 protein, partial (31%)
Length = 882
Score = 236 bits (601), Expect = 1e-62
Identities = 111/221 (50%), Positives = 151/221 (68%)
Frame = +2
Query: 30 LMFLVIIASSGSPIHAVQYQVTNRATGTPGGTRFENEIGIPFTQQTIQLASQFILQTFKQ 89
L FLV +++ + H V+Y VTN A TPGG F ++IG + ++T+ A+QFI + F+Q
Sbjct: 50 LCFLVFLSALHAT-HGVEYTVTNNALSTPGGVAFRDKIGAEYAKRTLDSATQFIWRIFQQ 226
Query: 90 IDQFGGKNIEHVSVIVTALNGNSAAITSDNNIFLDSNYIEGFSGDVRNEVIGILYHEATH 149
+ KN++ VS+ V ++G A TS+N I L + Y+ GDV+ E+ G+LYHE H
Sbjct: 227 NNPSDRKNVQKVSLFVDDMDG--VAYTSNNQIHLSARYVGNNKGDVKTEITGVLYHEMVH 400
Query: 150 IWQWFGNKEAPRGLTEGVADFMRLKAGFAPPHWVPRGTGSGWDEGYVVTAYFLDYCNGLR 209
+WQW GN AP GL EG+AD++RLKA +AP HWV G G WD+GY VTA FLDYC+ L+
Sbjct: 401 VWQWSGNGRAPGGLIEGIADYVRLKANYAPSHWVKAGQGQKWDQGYDVTARFLDYCDSLK 580
Query: 210 DGFVAMLNAMMKDHYSDDFFVKLLGKSVDQLWSDYKSVFGN 250
GFVA LN +M+ YSD +FV+LLGK VDQLW DYK+ +GN
Sbjct: 581 SGFVAQLNKLMRTGYSDQYFVQLLGKPVDQLWRDYKAKYGN 703
>TC228120 similar to UP|Q84ZV0 (Q84ZV0) R 14 protein, partial (31%)
Length = 1060
Score = 231 bits (589), Expect = 2e-61
Identities = 108/220 (49%), Positives = 148/220 (67%)
Frame = +3
Query: 30 LMFLVIIASSGSPIHAVQYQVTNRATGTPGGTRFENEIGIPFTQQTIQLASQFILQTFKQ 89
L FLV +A++ AV Y VTN A T GG RF + IG + +QT+ A+QFI +Q
Sbjct: 66 LCFLVFLAAAMQGTLAVDYTVTNNALSTSGGVRFRDAIGDDYAKQTLDSATQFIWGVLQQ 245
Query: 90 IDQFGGKNIEHVSVIVTALNGNSAAITSDNNIFLDSNYIEGFSGDVRNEVIGILYHEATH 149
K+++ V++ V ++G A TS++ I L + Y+ +SGD++ E+ G+LYHE H
Sbjct: 246 NAPADRKDVQKVNLFVDDMDG--VAYTSNDEIHLSARYVNDYSGDLKTEITGVLYHEMVH 419
Query: 150 IWQWFGNKEAPRGLTEGVADFMRLKAGFAPPHWVPRGTGSGWDEGYVVTAYFLDYCNGLR 209
+WQW GN + P GL EG+AD++RLKA +AP HWV G G WD+GY VTA+FLDYC+ L+
Sbjct: 420 VWQWNGNGQTPSGLIEGIADYVRLKANYAPSHWVKAGQGEKWDQGYDVTAHFLDYCDSLK 599
Query: 210 DGFVAMLNAMMKDHYSDDFFVKLLGKSVDQLWSDYKSVFG 249
GFVA LN M+D YSD FV+LLGK+VDQLW DYK+ +G
Sbjct: 600 SGFVAQLNQQMRDGYSDQLFVQLLGKTVDQLWQDYKAKYG 719
>NP595166 R 14 protein [Glycine max]
Length = 1926
Score = 223 bits (568), Expect = 6e-59
Identities = 101/205 (49%), Positives = 144/205 (69%), Gaps = 1/205 (0%)
Frame = +1
Query: 47 QYQVTNRATGTPGGTRFENEIGIPFTQQTIQLASQFILQTFKQIDQFGGKNIEHVSVIVT 106
++ V N A TPGG RF ++IG + +T++LA+QF+ + F+Q + KN++ VS++V
Sbjct: 1309 KFTVINNALTTPGGVRFRDKIGAEYANRTLELATQFVWRIFQQKNPSDRKNVQKVSLVVD 1488
Query: 107 ALNGNSAAITSDNNIFLDSNYIEGFSG-DVRNEVIGILYHEATHIWQWFGNKEAPRGLTE 165
+ G A T +N I + + Y+ G+SG DV+ E+ G+L+H+ ++WQW+GN EAP GL
Sbjct: 1489 DMEG--IAYTMNNEIHVSARYVNGYSGGDVKREITGVLFHQVCYVWQWYGNGEAPGGLIG 1662
Query: 166 GVADFMRLKAGFAPPHWVPRGTGSGWDEGYVVTAYFLDYCNGLRDGFVAMLNAMMKDHYS 225
G+ADF+RLKA +A HW G G WDEGY +TA+FLDYC+ L+ GFVA LN +M+ YS
Sbjct: 1663 GIADFVRLKANYAASHWRKPGQGQRWDEGYDITAHFLDYCDSLKSGFVAQLNQLMRTGYS 1842
Query: 226 DDFFVKLLGKSVDQLWSDYKSVFGN 250
D FFV+LLGK VDQLW DYK+ +GN
Sbjct: 1843 DQFFVQLLGKPVDQLWRDYKAQYGN 1917
>NP595167 R 13 protein [Glycine max]
Length = 1926
Score = 202 bits (513), Expect = 2e-52
Identities = 94/204 (46%), Positives = 135/204 (66%), Gaps = 1/204 (0%)
Frame = +1
Query: 48 YQVTNRATGTPGGTRFENEIGIPFTQQTIQLASQFILQTFKQIDQFGGKNIEHVSVIVTA 107
+ V N A T GG RF ++IG + QT+ A+QFI + F+Q + K+++ VS+ V
Sbjct: 1312 FTVINNALTTLGGVRFRDKIGAEYANQTLDSATQFIWEIFQQNNPSDRKSVQKVSLFVDD 1491
Query: 108 LNGNSAAITSDNNIFLDSNYIEGFSG-DVRNEVIGILYHEATHIWQWFGNKEAPRGLTEG 166
++G A T N I + + Y+ G+SG DV+ E+ G+L+H+ ++WQW GN +AP GLT G
Sbjct: 1492 MDG--IAYTRKNEIHVSARYVNGYSGGDVKREITGVLFHQVCYVWQWKGNGKAPGGLTGG 1665
Query: 167 VADFMRLKAGFAPPHWVPRGTGSGWDEGYVVTAYFLDYCNGLRDGFVAMLNAMMKDHYSD 226
+ADF+RLKA +A HW G G W+EGY +TA+FLDYC+ L+ GFV LN M+ YSD
Sbjct: 1666 IADFVRLKANYAASHWRKPGQGQKWNEGYEITAHFLDYCDSLKSGFVGQLNQWMRTDYSD 1845
Query: 227 DFFVKLLGKSVDQLWSDYKSVFGN 250
+FF LL K V+ LW DYK+++GN
Sbjct: 1846 EFFFLLLAKPVNHLWRDYKAMYGN 1917
>TC228121 similar to UP|Q84ZV0 (Q84ZV0) R 14 protein, partial (9%)
Length = 532
Score = 93.6 bits (231), Expect = 8e-20
Identities = 41/60 (68%), Positives = 48/60 (79%)
Frame = +3
Query: 191 WDEGYVVTAYFLDYCNGLRDGFVAMLNAMMKDHYSDDFFVKLLGKSVDQLWSDYKSVFGN 250
WD+GY VTA FLDYCN L+ GFVA LN M+ YSD FFV+LLGK+VDQLW DYK+ +GN
Sbjct: 3 WDQGYDVTARFLDYCNTLKSGFVAQLNKQMRTGYSDQFFVQLLGKTVDQLWQDYKAKYGN 182
>TC226225
Length = 730
Score = 31.2 bits (69), Expect = 0.46
Identities = 22/53 (41%), Positives = 28/53 (52%)
Frame = -1
Query: 7 ICCILSSCAALIYHWTKMSPIIYLMFLVIIASSGSPIHAVQYQVTNRATGTPG 59
I LSSC L Y T +SP + L V IAS G+ I + VT+ + G PG
Sbjct: 190 ISLTLSSCLQLGYFTTVLSPDLLLSVSVSIASDGAWI-LLTTSVTSCSKGMPG 35
>TC226224
Length = 1804
Score = 30.0 bits (66), Expect = 1.0
Identities = 22/53 (41%), Positives = 28/53 (52%)
Frame = -1
Query: 7 ICCILSSCAALIYHWTKMSPIIYLMFLVIIASSGSPIHAVQYQVTNRATGTPG 59
I LSSC L T MSP + L LV +AS G+ I + VT+ + G PG
Sbjct: 1231 ISLTLSSCLPLGSFATVMSPDLLLSILVSMASDGAWI-LLTTSVTSCSKGMPG 1076
>TC211281
Length = 501
Score = 29.6 bits (65), Expect = 1.4
Identities = 15/34 (44%), Positives = 20/34 (58%)
Frame = -3
Query: 7 ICCILSSCAALIYHWTKMSPIIYLMFLVIIASSG 40
ICC L S AA++ HW +M + LM V+ SG
Sbjct: 442 ICCSLFSTAAILSHWKEMWLSLGLMGAVLGMPSG 341
>TC223326
Length = 771
Score = 28.9 bits (63), Expect = 2.3
Identities = 9/23 (39%), Positives = 15/23 (65%)
Frame = -2
Query: 150 IWQWFGNKEAPRGLTEGVADFMR 172
+WQW NKE +++ ++ FMR
Sbjct: 287 LWQWVQNKETESAISKAISTFMR 219
>TC204617 similar to UP|Q9ASZ9 (Q9ASZ9) At1g60730/F8A5_24, partial (98%)
Length = 1579
Score = 28.1 bits (61), Expect = 3.9
Identities = 20/74 (27%), Positives = 34/74 (45%)
Frame = -2
Query: 55 TGTPGGTRFENEIGIPFTQQTIQLASQFILQTFKQIDQFGGKNIEHVSVIVTALNGNSAA 114
T +P R ++ +PF+ +L SQF L + + K E V V + +
Sbjct: 489 TSSPHICRITTDLSLPFSNVDSKLCSQFNLISHPSLQSLS*K--EFVGVRTIDVRSVKKS 316
Query: 115 ITSDNNIFLDSNYI 128
TS N++ +SN+I
Sbjct: 315 DTSLNSVVDESNHI 274
>TC208458
Length = 788
Score = 27.7 bits (60), Expect = 5.1
Identities = 15/36 (41%), Positives = 20/36 (54%)
Frame = -1
Query: 7 ICCILSSCAALIYHWTKMSPIIYLMFLVIIASSGSP 42
ICC L S AA++ HW +M +L +I S G P
Sbjct: 434 ICCSLFSTAAMLSHWKEM----WLSLGLIGTSLGMP 339
>BM523240
Length = 429
Score = 27.7 bits (60), Expect = 5.1
Identities = 17/76 (22%), Positives = 30/76 (39%), Gaps = 13/76 (17%)
Frame = +3
Query: 5 LPICCILSSCAAL-------------IYHWTKMSPIIYLMFLVIIASSGSPIHAVQYQVT 51
+P C ++ S A L I H + I+Y + + + + +G A+ Y
Sbjct: 117 IPYCVVICSFAKLGQLEMAEDIYWEMIRHGVQPDVIVYSILINVFSDAGRVKEAISYVDE 296
Query: 52 NRATGTPGGTRFENEI 67
+ G PG T N +
Sbjct: 297 MKKAGLPGNTVIYNSL 344
>TC214245 similar to UP|Q944G1 (Q944G1) Phosphomevalonate kinase, partial
(35%)
Length = 1769
Score = 27.3 bits (59), Expect = 6.7
Identities = 10/24 (41%), Positives = 15/24 (61%), Gaps = 4/24 (16%)
Frame = +1
Query: 4 KLPICCILSS----CAALIYHWTK 23
K PICC+ SS C + Y+W++
Sbjct: 805 KFPICCVTSSCLFRCTYVFYNWSR 876
>TC205836
Length = 1114
Score = 26.9 bits (58), Expect = 8.8
Identities = 16/43 (37%), Positives = 20/43 (46%)
Frame = +2
Query: 179 PPHWVPRGTGSGWDEGYVVTAYFLDYCNGLRDGFVAMLNAMMK 221
PP VPRG G + A ++ LRDGFV M M+
Sbjct: 530 PPGIVPRGGGVKRERERDAVAEMVEAIKVLRDGFVRMEQMKME 658
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.323 0.139 0.438
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,491,205
Number of Sequences: 63676
Number of extensions: 148342
Number of successful extensions: 798
Number of sequences better than 10.0: 28
Number of HSP's better than 10.0 without gapping: 794
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 796
length of query: 250
length of database: 12,639,632
effective HSP length: 95
effective length of query: 155
effective length of database: 6,590,412
effective search space: 1021513860
effective search space used: 1021513860
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 58 (26.9 bits)
Lotus: description of TM0327.8