BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0327.1
(91 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC206158 131 6e-32
TC228040 similar to UP|AGM1_ARATH (P57750) Probable phosphoacety... 27 1.7
TC224879 homologue to gb|AF223066.1|AF223066 Humulus lupulus 26S... 26 2.9
TC227800 similar to GB|AAO42902.1|28416743|BT004656 At3g13845 {A... 26 2.9
TC216321 similar to UP|Q948Y7 (Q948Y7) VMP3 protein, partial (3%) 26 2.9
TC212457 UP|Q39871 (Q39871) Maturation polypeptide, complete 25 6.5
BE661660 PIR|S27757|S27 embryonic abundant protein 59K - soybea... 25 6.5
TC224925 homologue to UP|Q43453 (Q43453) G.max mRNA from stress-... 25 8.5
TC224991 homologue to UP|S25K_SOYBN (P10742) Stem 31 kDa glycopr... 25 8.5
TC215063 similar to UP|MAOX_VITVI (P51615) NADP-dependent malic ... 25 8.5
CK606126 25 8.5
TC227068 similar to UP|Q6UV11 (Q6UV11) Pseudo-response regulator... 25 8.5
TC224923 emb|X02623.1|GMRN18S Soybean (Glycine max) 18S ribosoma... 25 8.5
TC215061 similar to UP|O24550 (O24550) Malate dehydrogenase , p... 25 8.5
TC220732 similar to UP|Q6PMI1 (Q6PMI1) NADP-dependent malic enzy... 25 8.5
TC223034 similar to UP|Q6NM16 (Q6NM16) At5g27710, partial (12%) 25 8.5
TC221799 similar to UP|Q8LP44 (Q8LP44) Betaine/proline transport... 25 8.5
>TC206158
Length = 820
Score = 131 bits (329), Expect = 6e-32
Identities = 63/81 (77%), Positives = 68/81 (83%)
Frame = +3
Query: 9 LVLMQLRVDGVLIRLRDTRMHCVFGGSTNPVILRESCWRESTFQALSAKGTPFDPAVYND 68
L+ LRVDGVLIRLR+TRMHCVFGGSTNP+ILRESCWRESTFQALSAKG PFD Y D
Sbjct: 315 LLRFWLRVDGVLIRLRETRMHCVFGGSTNPIILRESCWRESTFQALSAKGHPFDSGAYGD 494
Query: 69 PSIISQRLPIVKQMTQNLVIS 89
PS ISQ+LPI+ TQ LVIS
Sbjct: 495 PSFISQKLPIIMHKTQKLVIS 557
>TC228040 similar to UP|AGM1_ARATH (P57750) Probable phosphoacetylglucosamine
mutase (PAGM) (Acetylglucosamine phosphomutase)
(N-acetylglucosamine-phosphate mutase)
(DNA-damage-repair/toleration protein DRT101) , partial
(51%)
Length = 1671
Score = 26.9 bits (58), Expect = 1.7
Identities = 12/25 (48%), Positives = 13/25 (52%)
Frame = -2
Query: 35 STNPVILRESCWRESTFQALSAKGT 59
STN I R CW E SAKG+
Sbjct: 905 STNSFINRRRCWGEGALARASAKGS 831
>TC224879 homologue to gb|AF223066.1|AF223066 Humulus lupulus 26S ribosomal RNA
gene, partial sequence; 18S ribosomal RNA gene, internal
transcribed spacer 1, 5.8S ribosomal RNA gene, internal
transcribed spacer 2 and 26S ribosomal RNA gene, complete
sequence; and 18S ribosomal RNA gene, partial sequence,
partial (5%)
Length = 1911
Score = 26.2 bits (56), Expect = 2.9
Identities = 13/38 (34%), Positives = 18/38 (47%)
Frame = -2
Query: 33 GGSTNPVILRESCWRESTFQALSAKGTPFDPAVYNDPS 70
GG+T PV R + E T + + G P DP P+
Sbjct: 1229 GGTTRPVKDRSASPAEGTSRPVHTGGGPIDPGTTKAPT 1116
>TC227800 similar to GB|AAO42902.1|28416743|BT004656 At3g13845 {Arabidopsis
thaliana;} , partial (74%)
Length = 742
Score = 26.2 bits (56), Expect = 2.9
Identities = 10/19 (52%), Positives = 12/19 (62%)
Frame = +1
Query: 32 FGGSTNPVILRESCWREST 50
+GG TN I SCWR S+
Sbjct: 199 YGGKTNSCIKGYSCWRHSS 255
>TC216321 similar to UP|Q948Y7 (Q948Y7) VMP3 protein, partial (3%)
Length = 661
Score = 26.2 bits (56), Expect = 2.9
Identities = 10/23 (43%), Positives = 15/23 (64%)
Frame = -3
Query: 35 STNPVILRESCWRESTFQALSAK 57
ST ++R S WRES +A+S +
Sbjct: 173 STKTALVRRSFWRESRLEAISVR 105
>TC212457 UP|Q39871 (Q39871) Maturation polypeptide, complete
Length = 1741
Score = 25.0 bits (53), Expect = 6.5
Identities = 11/22 (50%), Positives = 14/22 (63%)
Frame = -1
Query: 36 TNPVILRESCWRESTFQALSAK 57
+NP IL S W S+ +LSAK
Sbjct: 604 SNPCILHPSPWSSSSLPSLSAK 539
>BE661660 PIR|S27757|S27 embryonic abundant protein 59K - soybean, partial
(42%)
Length = 1025
Score = 25.0 bits (53), Expect = 6.5
Identities = 11/22 (50%), Positives = 14/22 (63%)
Frame = -2
Query: 36 TNPVILRESCWRESTFQALSAK 57
+NP IL S W S+ +LSAK
Sbjct: 592 SNPCILHPSPWSSSSLPSLSAK 527
>TC224925 homologue to UP|Q43453 (Q43453) G.max mRNA from stress-induced
gene (H4), complete
Length = 1041
Score = 24.6 bits (52), Expect = 8.5
Identities = 12/31 (38%), Positives = 16/31 (50%)
Frame = +1
Query: 33 GGSTNPVILRESCWRESTFQALSAKGTPFDP 63
GG+T PV R + E T + + G P DP
Sbjct: 16 GGTTRPVKDRSASPAEGTSRPVHTGGGPIDP 108
>TC224991 homologue to UP|S25K_SOYBN (P10742) Stem 31 kDa glycoprotein
precursor (Vegetative storage protein VSP25)
(Fragment), partial (16%)
Length = 904
Score = 24.6 bits (52), Expect = 8.5
Identities = 12/31 (38%), Positives = 16/31 (50%)
Frame = +2
Query: 33 GGSTNPVILRESCWRESTFQALSAKGTPFDP 63
GG+T PV R + E T + + G P DP
Sbjct: 14 GGTTRPVKDRSASPAEGTSRPVHTGGGPIDP 106
>TC215063 similar to UP|MAOX_VITVI (P51615) NADP-dependent malic enzyme
(NADP-ME) , partial (32%)
Length = 918
Score = 24.6 bits (52), Expect = 8.5
Identities = 10/24 (41%), Positives = 15/24 (61%)
Frame = +2
Query: 43 ESCWRESTFQALSAKGTPFDPAVY 66
E ++ S +A+ A G+PFDP Y
Sbjct: 173 EEAYQWSEGRAIFASGSPFDPVEY 244
>CK606126
Length = 592
Score = 24.6 bits (52), Expect = 8.5
Identities = 12/31 (38%), Positives = 16/31 (50%)
Frame = +1
Query: 33 GGSTNPVILRESCWRESTFQALSAKGTPFDP 63
GG+T PV R + E T + + G P DP
Sbjct: 16 GGTTRPVKDRSASPAEGTSRPVHTGGGPIDP 108
>TC227068 similar to UP|Q6UV11 (Q6UV11) Pseudo-response regulator protein
(Fragment), partial (26%)
Length = 1737
Score = 24.6 bits (52), Expect = 8.5
Identities = 10/28 (35%), Positives = 15/28 (52%)
Frame = -3
Query: 27 RMHCVFGGSTNPVILRESCWRESTFQAL 54
R+HC T+ +L+ SC E +Q L
Sbjct: 559 RLHCTLRKQTDQNVLKHSCHPELQYQHL 476
>TC224923 emb|X02623.1|GMRN18S Soybean (Glycine max) 18S ribosomal RNA,
complete
Length = 3227
Score = 24.6 bits (52), Expect = 8.5
Identities = 12/31 (38%), Positives = 16/31 (50%)
Frame = -1
Query: 33 GGSTNPVILRESCWRESTFQALSAKGTPFDP 63
GG+T PV R + E T + + G P DP
Sbjct: 1502 GGTTRPVKDRSASPAEGTSRPVHTGGGPIDP 1410
>TC215061 similar to UP|O24550 (O24550) Malate dehydrogenase , partial (58%)
Length = 1146
Score = 24.6 bits (52), Expect = 8.5
Identities = 10/24 (41%), Positives = 15/24 (61%)
Frame = +1
Query: 43 ESCWRESTFQALSAKGTPFDPAVY 66
E ++ S +A+ A G+PFDP Y
Sbjct: 910 EEAYQWSEGRAIFASGSPFDPVEY 981
>TC220732 similar to UP|Q6PMI1 (Q6PMI1) NADP-dependent malic enzyme 3 ,
partial (32%)
Length = 662
Score = 24.6 bits (52), Expect = 8.5
Identities = 10/24 (41%), Positives = 15/24 (61%)
Frame = +1
Query: 43 ESCWRESTFQALSAKGTPFDPAVY 66
E ++ S +A+ A G+PFDP Y
Sbjct: 181 EEAYQWSEGRAIFASGSPFDPVEY 252
>TC223034 similar to UP|Q6NM16 (Q6NM16) At5g27710, partial (12%)
Length = 568
Score = 24.6 bits (52), Expect = 8.5
Identities = 14/33 (42%), Positives = 19/33 (57%), Gaps = 3/33 (9%)
Frame = +3
Query: 62 DPAVYN---DPSIISQRLPIVKQMTQNLVISPK 91
DPA + DP+II R IVK ++ V SP+
Sbjct: 363 DPAAIHRLLDPTIIPHRYLIVKDQRKSAVSSPE 461
>TC221799 similar to UP|Q8LP44 (Q8LP44) Betaine/proline transporter,
partial (26%)
Length = 398
Score = 24.6 bits (52), Expect = 8.5
Identities = 9/18 (50%), Positives = 14/18 (77%)
Frame = -2
Query: 46 WRESTFQALSAKGTPFDP 63
+++S Q L ++GTPFDP
Sbjct: 79 YQDSK*QVLESQGTPFDP 26
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.331 0.144 0.435
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,594,274
Number of Sequences: 63676
Number of extensions: 38305
Number of successful extensions: 252
Number of sequences better than 10.0: 34
Number of HSP's better than 10.0 without gapping: 252
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 252
length of query: 91
length of database: 12,639,632
effective HSP length: 67
effective length of query: 24
effective length of database: 8,373,340
effective search space: 200960160
effective search space used: 200960160
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.9 bits)
S2: 52 (24.6 bits)
Lotus: description of TM0327.1