BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0302a.1
(299 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BE329487 weakly similar to PIR|T14619|T146 reverse transcriptase... 90 1e-18
BI321779 87 9e-18
TC218847 81 7e-16
TC213164 81 7e-16
TC206673 76 2e-14
CA936755 48 5e-06
TC234193 42 4e-04
CA851030 37 0.008
BI968991 37 0.011
BE474579 28 0.24
CD409155 32 0.45
TC234094 31 0.59
BM732560 30 1.3
CA800510 30 1.3
TC220662 weakly similar to UP|RNP1_YEAST (P32385) Ribonucleoprot... 29 2.3
TC228190 28 5.0
BG405621 28 5.0
TC225348 homologue to UP|KPPR_MESCR (P27774) Phosphoribulokinase... 28 5.0
TC212583 28 5.0
TC205647 similar to UP|Q8GU23 (Q8GU23) Orcinol O-methyltransfera... 28 5.0
>BE329487 weakly similar to PIR|T14619|T146 reverse transcriptase - beet
retrotransposon (fragment), partial (15%)
Length = 376
Score = 89.7 bits (221), Expect = 1e-18
Identities = 43/88 (48%), Positives = 60/88 (67%), Gaps = 12/88 (13%)
Frame = -2
Query: 212 IRTAVWECEGDRSLGPDVL------------KVDILRVLKDFHRNGVWPKGGNSSFITLI 259
I++AVW+C +S GPD L K DI+R L +FH NG++PKGGN+SFI LI
Sbjct: 375 IKSAVWQCGSVKSPGPDGLNFKFIKHFWERLKPDIIRFLNEFHANGIFPKGGNASFIALI 196
Query: 260 PNISNLMSLNDYISISVIGCMYKIVSKV 287
P + + +LND+ IS+IGC+YKIV+K+
Sbjct: 195 PKVKHPQALNDFRPISLIGCVYKIVAKI 112
>BI321779
Length = 421
Score = 87.0 bits (214), Expect = 9e-18
Identities = 47/126 (37%), Positives = 67/126 (52%), Gaps = 12/126 (9%)
Frame = +2
Query: 173 FEAQFTSNPEVGVFLEGTPFRSISDEDNLALTQTFNLEEIRTAVWECEGDRSLGPD---- 228
F+ Q P+ L G F S S+EDN L Q F +EEI+ + +CE +S GPD
Sbjct: 5 FQEQIYGRPK----LNGVEFLSFSNEDNAMLIQDFEVEEIKKVI*DCESSKSPGPDGYSL 172
Query: 229 --------VLKVDILRVLKDFHRNGVWPKGGNSSFITLIPNISNLMSLNDYISISVIGCM 280
V+K +++ K+FH+ P+G N SFI LI N + + Y IS++GC+
Sbjct: 173 LLIKIFWEVIKEEVINFFKEFHKFDFLPRGTNRSFIALIAKCDNPLDIGQYCPISLVGCL 352
Query: 281 YKIVSK 286
YKIV K
Sbjct: 353 YKIV*K 370
>TC218847
Length = 521
Score = 80.9 bits (198), Expect = 7e-16
Identities = 36/98 (36%), Positives = 61/98 (61%)
Frame = -3
Query: 103 LVEYWKVAKFNDSLLFQKSRDRWVKDGDSNTKYFHNSVNWRRRTNAIRGLAVDGEWVEDP 162
++ W A+ ++SLL QK+R +W+++ D N+ YFH +N+ RTNA+ G+ + G WVE+P
Sbjct: 294 IIMEWTAAQAHESLLRQKARTKWIRERDCNSSYFHKLINYIHRTNAVNGVMIGGSWVEEP 115
Query: 163 KVVKSKMIEFFEAQFTSNPEVGVFLEGTPFRSISDEDN 200
VK+ + FF+ +F+ + + G FRSI + N
Sbjct: 114 NRVKA-VRNFFQIRFSESDYDRP*INGANFRSIGQQQN 4
>TC213164
Length = 446
Score = 80.9 bits (198), Expect = 7e-16
Identities = 43/106 (40%), Positives = 64/106 (59%), Gaps = 12/106 (11%)
Frame = +3
Query: 203 LTQTFNLEEIRTAVWECEGDRSLGPD------------VLKVDILRVLKDFHRNGVWPKG 250
L F +E+R +W+ +S GPD +LK D+LR L +F+ NGV+ K
Sbjct: 18 LVARFEKKEVRATMWDRGNAKSPGPDGLNFKFIKEFWDILKTDLLRFLDEFYANGVFLKR 197
Query: 251 GNSSFITLIPNISNLMSLNDYISISVIGCMYKIVSKVLTTRLSKVM 296
N+ F+ LIP + + SLN+Y IS+IGC+YKIV+K+L+ R KV+
Sbjct: 198 SNAFFLALIPKVHDP*SLNEYKPISLIGCIYKIVAKLLSGRHKKVL 335
>TC206673
Length = 742
Score = 76.3 bits (186), Expect = 2e-14
Identities = 31/93 (33%), Positives = 59/93 (63%)
Frame = +3
Query: 78 RINALDIKEGDGNLSSQEQKQRDDFLVEYWKVAKFNDSLLFQKSRDRWVKDGDSNTKYFH 137
++N ++ + +LS E K + + + W+ + +SLL QKSRD+W+K+GD+N+ YFH
Sbjct: 39 KLNEVEDLASNRSLSVDEIKAKKELQQQLWEASTAYESLLRQKSRDKWLKEGDNNSAYFH 218
Query: 138 NSVNWRRRTNAIRGLAVDGEWVEDPKVVKSKMI 170
+N+RR N ++G+ + GEW+ P + ++ +
Sbjct: 219 TVINFRRHYNGLQGILIQGEWIALPGLALAQQL 317
>CA936755
Length = 424
Score = 48.1 bits (113), Expect = 5e-06
Identities = 23/53 (43%), Positives = 28/53 (52%), Gaps = 1/53 (1%)
Frame = -2
Query: 12 KPFRNRNCWLQNHAFGECV-STWSSFDIQGWGAYTFKEKLKRLKLKLNQWEKE 63
KPFR + WL + V TWS+ GWG K KL+ LKL + QW KE
Sbjct: 174 KPFRVVDWWLHQKGYQRLVRETWSAEQQPGWGGILLKNKLRMLKLSIKQWSKE 16
>TC234193
Length = 697
Score = 41.6 bits (96), Expect = 4e-04
Identities = 31/111 (27%), Positives = 58/111 (51%), Gaps = 13/111 (11%)
Frame = -3
Query: 185 VFLEGTPFRSISDEDNLALTQTFNL--EEIRTAV---WECEGDRSLGP--------DVLK 231
V L+G F++I+ EDN LT L + R ++ W+ + D + +V+K
Sbjct: 326 VLLDGVNFKTINGEDNDRLTHVLVLGTQNKRGSLGV*WKQKFDSNGYNFNFIKKC*EVMK 147
Query: 232 VDILRVLKDFHRNGVWPKGGNSSFITLIPNISNLMSLNDYISISVIGCMYK 282
D++R +++FH +G +G N+SF+T P L D+ ++++G + K
Sbjct: 146 EDVVRAIQEFHSHGCLSRGTNASFLTHPP-----*GLGDFRPVTLVGSLCK 9
>CA851030
Length = 358
Score = 37.4 bits (85), Expect = 0.008
Identities = 21/70 (30%), Positives = 35/70 (50%), Gaps = 1/70 (1%)
Frame = +1
Query: 105 EYWKVAKFNDSLLFQKSRDRWVKDGDSNTKYFHNSVNWRRRTNAIRGLAVD-GEWVEDPK 163
EYW+ Q+SR W K+ D NT++FH R+ +N+I + D G P
Sbjct: 178 EYWQ----------QRSRAIWFKERDKNTEFFHRKTLQRKASNSITKIQDDEGNVCFKPD 327
Query: 164 VVKSKMIEFF 173
++ +I+F+
Sbjct: 328 QIEDILIKFY 357
>BI968991
Length = 750
Score = 37.0 bits (84), Expect = 0.011
Identities = 15/25 (60%), Positives = 18/25 (72%)
Frame = +1
Query: 113 NDSLLFQKSRDRWVKDGDSNTKYFH 137
ND L F+KS +W+KDGD T YFH
Sbjct: 16 NDILWFRKS*AKWIKDGDHETGYFH 90
>BE474579
Length = 419
Score = 28.1 bits (61), Expect(2) = 0.24
Identities = 21/57 (36%), Positives = 31/57 (53%)
Frame = +3
Query: 105 EYWKVAKFNDSLLFQKSRDRWVKDGDSNTKYFHNSVNWRRRTNAIRGLAVDGEWVED 161
E+WKV+K+ +LF + V S + F + +N R TNAI GL ++ W ED
Sbjct: 6 EFWKVSKWYI*ILFSTKIKKQVN*--S*GRQFVHMMN*SR-TNAIMGLLMEECWEED 167
Score = 23.1 bits (48), Expect(2) = 0.24
Identities = 12/39 (30%), Positives = 20/39 (50%)
Frame = +1
Query: 165 VKSKMIEFFEAQFTSNPEVGVFLEGTPFRSISDEDNLAL 203
VK + +FF+ + L G PF +I++E+N L
Sbjct: 175 VKMGVKQFFQIGLMKVVGRDLVLSGLPFTTIAEEENRML 291
>CD409155
Length = 349
Score = 31.6 bits (70), Expect = 0.45
Identities = 16/37 (43%), Positives = 24/37 (64%), Gaps = 1/37 (2%)
Frame = -2
Query: 233 DILRVLKDFHRNGVWPKG-GNSSFITLIPNISNLMSL 268
DILR LKDF R+GV+P+ + F+ N++N S+
Sbjct: 327 DILRFLKDFCRHGVFPRAMASMPFLDASFNVNNTFSI 217
>TC234094
Length = 457
Score = 31.2 bits (69), Expect = 0.59
Identities = 13/35 (37%), Positives = 17/35 (48%)
Frame = -3
Query: 11 LKPFRNRNCWLQNHAFGECVSTWSSFDIQGWGAYT 45
L P R R CW G+C S WS +++ W T
Sbjct: 107 LSPLRLRWCWKPTQERGQC*SCWSLENLRPWSKQT 3
>BM732560
Length = 422
Score = 30.0 bits (66), Expect = 1.3
Identities = 11/49 (22%), Positives = 26/49 (52%), Gaps = 1/49 (2%)
Frame = -2
Query: 119 QKSRDRWVKDGDSNTKYFH-NSVNWRRRTNAIRGLAVDGEWVEDPKVVK 166
Q +R +W++DG+ N +++ ++ RR+ + G W ++ + K
Sbjct: 169 QNTRGQWIRDGERNNRHYKIKTITGRRKNRILMSRGH*GTWADEHPI*K 23
>CA800510
Length = 409
Score = 30.0 bits (66), Expect = 1.3
Identities = 14/45 (31%), Positives = 23/45 (51%)
Frame = -2
Query: 97 KQRDDFLVEYWKVAKFNDSLLFQKSRDRWVKDGDSNTKYFHNSVN 141
+QR F E+W + +S LFQK +W+ G + F ++ N
Sbjct: 195 EQRSVFQQEFWAFTRQKESSLFQKWHPKWIN*GTTILNIFISA*N 61
>TC220662 weakly similar to UP|RNP1_YEAST (P32385) Ribonucleoprotein 1,
partial (8%)
Length = 1128
Score = 29.3 bits (64), Expect = 2.3
Identities = 16/40 (40%), Positives = 20/40 (50%), Gaps = 2/40 (5%)
Frame = +3
Query: 115 SLLFQKSRDRWVKDG--DSNTKYFHNSVNWRRRTNAIRGL 152
SLL K R RW+K+G + N WR + N RGL
Sbjct: 24 SLL*GKLRRRWLKNGFTEKNMSTLETMTFWRTKKNETRGL 143
>TC228190
Length = 877
Score = 28.1 bits (61), Expect = 5.0
Identities = 19/66 (28%), Positives = 29/66 (43%)
Frame = +3
Query: 15 RNRNCWLQNHAFGECVSTWSSFDIQGWGAYTFKEKLKRLKLKLNQWEKENVGDLDKICKD 74
R + WL ++ GE +TW G + LK + ++ EK G L K+ K
Sbjct: 276 RTCSMWLSSYLTGESATTWPRLT----GMKFLTKSLKSISTRMEDMEKHG-GLLLKMAKS 440
Query: 75 PVTRIN 80
+ RIN
Sbjct: 441 FLLRIN 458
>BG405621
Length = 402
Score = 28.1 bits (61), Expect = 5.0
Identities = 10/20 (50%), Positives = 16/20 (80%)
Frame = +2
Query: 119 QKSRDRWVKDGDSNTKYFHN 138
Q+S+ W+K GD N+K+FH+
Sbjct: 341 QRSKIFWLKYGDLNSKFFHS 400
>TC225348 homologue to UP|KPPR_MESCR (P27774) Phosphoribulokinase,
chloroplast precursor (Phosphopentokinase) (PRKASE)
(PRK) , partial (91%)
Length = 1776
Score = 28.1 bits (61), Expect = 5.0
Identities = 13/31 (41%), Positives = 19/31 (60%)
Frame = +3
Query: 248 PKGGNSSFITLIPNISNLMSLNDYISISVIG 278
PKGGN TLI + + ++ L+DY S+ G
Sbjct: 498 PKGGNPDSNTLISDTTTVICLDDYHSLDRTG 590
>TC212583
Length = 530
Score = 28.1 bits (61), Expect = 5.0
Identities = 9/18 (50%), Positives = 14/18 (77%)
Frame = +2
Query: 124 RWVKDGDSNTKYFHNSVN 141
+W +GD N+K+FH+ VN
Sbjct: 2 KWFLEGDHNSKFFHSYVN 55
>TC205647 similar to UP|Q8GU23 (Q8GU23) Orcinol O-methyltransferase, partial
(89%)
Length = 1774
Score = 28.1 bits (61), Expect = 5.0
Identities = 25/83 (30%), Positives = 37/83 (44%), Gaps = 8/83 (9%)
Frame = +2
Query: 53 LKLKLNQWEKENVGDLDKICKDPVTRINALDIKEGDGNLSSQ--EQKQRDDFLVEYWKVA 110
LK L+ W + D+ K CK+ +TR KEG + E ++RDD VE
Sbjct: 1037 LKWILHDWNDKECVDILKKCKEAITRKG----KEGKVIIIDMVVENEKRDDESVE---TQ 1195
Query: 111 KFNDSLLF------QKSRDRWVK 127
F D L+ ++S+ W K
Sbjct: 1196 LFFDMLMMVLVTGKERSKKEWAK 1264
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.325 0.140 0.445
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 14,040,559
Number of Sequences: 63676
Number of extensions: 193075
Number of successful extensions: 1213
Number of sequences better than 10.0: 45
Number of HSP's better than 10.0 without gapping: 1205
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1207
length of query: 299
length of database: 12,639,632
effective HSP length: 97
effective length of query: 202
effective length of database: 6,463,060
effective search space: 1305538120
effective search space used: 1305538120
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 59 (27.3 bits)
Lotus: description of TM0302a.1