BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0301a.4
(147 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
NP595172 polyprotein [Glycine max] 107 2e-24
TC211627 88 1e-18
TC211973 weakly similar to UP|Q84ZV5 (Q84ZV5) Polyprotein, parti... 61 5e-17
TC213413 weakly similar to UP|Q84ZV5 (Q84ZV5) Polyprotein, parti... 83 5e-17
BM731326 weakly similar to GP|21740635|em OSJNBb0043H09.2 {Oryza... 80 5e-16
TC212592 weakly similar to UP|Q84ZV5 (Q84ZV5) Polyprotein, parti... 79 1e-15
TC213114 weakly similar to UP|Q8W150 (Q8W150) Polyprotein, parti... 69 1e-12
BE807407 42 1e-07
BU549069 44 2e-05
TC204929 similar to GB|AAD39665.1|5103835|F9L1 ESTs gb|T22508, g... 42 1e-04
BQ272766 weakly similar to GP|28558781|gb| pol protein {Cucumis ... 36 0.008
BE211654 33 0.038
AW348690 30 0.32
BU578017 30 0.42
TC218053 weakly similar to UP|Q6I621 (Q6I621) Protein phosphatas... 30 0.55
TC211469 29 0.94
BU547665 29 0.94
CA784736 28 1.6
BG790334 weakly similar to GP|2160154|gb| F19K23.18 gene product... 27 4.6
BM521595 26 7.9
>NP595172 polyprotein [Glycine max]
Length = 4659
Score = 107 bits (268), Expect = 2e-24
Identities = 54/146 (36%), Positives = 80/146 (53%)
Frame = +1
Query: 1 VFIKLMALRENSVVTRDCPQLTAPYYGPYPIIQRIGAVAYRLQLPEGGRVHPVFHASLLK 60
V +KL R++S V R +L+ Y+GP+ ++ +IG VAY+L+LP R+HPVFH S LK
Sbjct: 4072 VLVKLQPYRQHSAVLRKNQKLSMRYFGPFKVLAKIGDVAYKLELPSAARIHPVFHVSQLK 4251
Query: 61 EAVGNNSVELQLLDHLTGEEVASVHPFSVITSRFTTRQESTLPQVWIQWQGKPADEPTWK 120
G L E + P ++ SR R + + Q+ +QW+ DE TW+
Sbjct: 4252 PFNGTAQDPYLPLPLTVTEMGPVMQPVKILASRIIIRGHNQIEQILVQWENGLQDEATWE 4431
Query: 121 DTLNIRSQFPVFNLEDKVDLSAGGIV 146
D +I++ +P FNLEDKV G V
Sbjct: 4432 DIEDIKASYPTFNLEDKVVFKGEGNV 4509
>TC211627
Length = 1034
Score = 88.2 bits (217), Expect = 1e-18
Identities = 49/145 (33%), Positives = 75/145 (50%)
Frame = +3
Query: 1 VFIKLMALRENSVVTRDCPQLTAPYYGPYPIIQRIGAVAYRLQLPEGGRVHPVFHASLLK 60
V+++L R+ S+ + +L+ +YGPY I R+G VAYRLQLP ++HP+FH SLLK
Sbjct: 438 VYVRL*PYRQTSIQST-YTKLSKRFYGPYQIQARVGQVAYRLQLPPTSKIHPIFHVSLLK 614
Query: 61 EAVGNNSVELQLLDHLTGEEVASVHPFSVITSRFTTRQESTLPQVWIQWQGKPADEPTWK 120
G EL L + V P + + +PQV +QW ++ TW+
Sbjct: 615 VHHGPIPPELLALPPFSTTNHPLVQPLQFLDWKMDESTTPPIPQVLVQWTNLAPEDTTWE 794
Query: 121 DTLNIRSQFPVFNLEDKVDLSAGGI 145
++ +++LEDKV GGI
Sbjct: 795 SWTQLKD---IYDLEDKVCFQTGGI 860
>TC211973 weakly similar to UP|Q84ZV5 (Q84ZV5) Polyprotein, partial (4%)
Length = 730
Score = 61.2 bits (147), Expect(2) = 5e-17
Identities = 26/65 (40%), Positives = 41/65 (63%)
Frame = +1
Query: 1 VFIKLMALRENSVVTRDCPQLTAPYYGPYPIIQRIGAVAYRLQLPEGGRVHPVFHASLLK 60
VF+K+ R S+ R +L+ +Y P+ + ++G +AY+L LP ++HPVFH SLLK
Sbjct: 154 VFLKMQPYRRRSLAKRINEKLSPRFYAPFQVFNKVGTIAYKLDLPSHIKIHPVFHVSLLK 333
Query: 61 EAVGN 65
+A N
Sbjct: 334 KASPN 348
Score = 42.0 bits (97), Expect(2) = 5e-17
Identities = 17/42 (40%), Positives = 26/42 (61%)
Frame = +3
Query: 104 QVWIQWQGKPADEPTWKDTLNIRSQFPVFNLEDKVDLSAGGI 145
+V IQW+ P E +W+ ++ F +++LEDKV L GGI
Sbjct: 438 KVLIQWKNLPPSENSWESVAKLQEIFSIYHLEDKVSLLGGGI 563
>TC213413 weakly similar to UP|Q84ZV5 (Q84ZV5) Polyprotein, partial (5%)
Length = 761
Score = 82.8 bits (203), Expect = 5e-17
Identities = 49/139 (35%), Positives = 71/139 (50%), Gaps = 1/139 (0%)
Frame = +1
Query: 1 VFIKLMALRENSVVTRDCPQLTAPYYGPYPIIQRIGAVAYRLQLPEGGRVHPVFHASLLK 60
V +KL R+ S +LT YYGP+ + +R+G V YRL+L R+HPVFH SLLK
Sbjct: 16 VLVKLRPHRQGSASETTYSKLTKRYYGPFEVQERLGKVVYRLKLTAHSRIHPVFHVSLLK 195
Query: 61 EAVGN-NSVELQLLDHLTGEEVASVHPFSVITSRFTTRQESTLPQVWIQWQGKPADEPTW 119
VG+ + L + EE + P +VI S+ V +QW + +W
Sbjct: 196 AFVGDPETTHAGPLPVMRTEEATNT-PLTVIDSKLVPADNGPRRMVLVQWPSASRQDASW 372
Query: 120 KDTLNIRSQFPVFNLEDKV 138
+D +R + +NLEDKV
Sbjct: 373 EDWQVLRER---YNLEDKV 420
>BM731326 weakly similar to GP|21740635|em OSJNBb0043H09.2 {Oryza sativa
(japonica cultivar-group)}, partial (3%)
Length = 424
Score = 79.7 bits (195), Expect = 5e-16
Identities = 43/109 (39%), Positives = 68/109 (61%), Gaps = 1/109 (0%)
Frame = +1
Query: 1 VFIKLMALRENSVVTRDCPQLTAPYYGPYPIIQRIGAVAYRLQLPEGGRVHPVFHASLLK 60
V++K+ R+ S+ R P+LTA +YGPY +++++GAVA++LQLP R+HPVFH S LK
Sbjct: 103 VYLKIRPHRQGSMPPRLHPKLTARFYGPYLVMRQVGAVAFQLQLPSEARIHPVFHVSQLK 282
Query: 61 EAVGNNSVELQLLDHLTGEEVASVH-PFSVITSRFTTRQESTLPQVWIQ 108
A+GN+ + +L L E A ++ P ++ R +Q QV I+
Sbjct: 283 RALGNHQAQEELPPDL--EHQAELYFPVQILKIREVQKQHEVERQVLIR 423
>TC212592 weakly similar to UP|Q84ZV5 (Q84ZV5) Polyprotein, partial (4%)
Length = 664
Score = 78.6 bits (192), Expect = 1e-15
Identities = 41/119 (34%), Positives = 66/119 (55%)
Frame = +2
Query: 20 QLTAPYYGPYPIIQRIGAVAYRLQLPEGGRVHPVFHASLLKEAVGNNSVELQLLDHLTGE 79
+L+ ++GP+ +++R+G VAYRLQLP ++HPVFH SLLK GN L +
Sbjct: 74 KLSKRFFGPFQVVERVGKVAYRLQLPVDAKLHPVFHCSLLKPFQGNPPDTAAPLPPTLFD 253
Query: 80 EVASVHPFSVITSRFTTRQESTLPQVWIQWQGKPADEPTWKDTLNIRSQFPVFNLEDKV 138
+ + P ++ +R + +V +QWQG D+ TW+ + + F+LEDKV
Sbjct: 254 HQSVIAPLVILATRTVNDDDI---EVLVQWQGLSPDDATWEKWTELCKE---FHLEDKV 412
>TC213114 weakly similar to UP|Q8W150 (Q8W150) Polyprotein, partial (7%)
Length = 810
Score = 68.6 bits (166), Expect = 1e-12
Identities = 48/141 (34%), Positives = 74/141 (52%), Gaps = 4/141 (2%)
Frame = +3
Query: 1 VFIKLMALRENSVVTRDCPQLTAPYYGPYPIIQRIGAVAYRLQLPEGGRVHPVFHASLLK 60
V++KL R S+ + +L+ +YGPY I ++IG VA+ L LP ++HPVFHASLLK
Sbjct: 60 VYLKLQPYRLKSLAKKRNEKLSPRFYGPYQIKKQIGLVAFELDLPPARKIHPVFHASLLK 239
Query: 61 EAVGNNSVELQLLDHLTGEEVASVHPFSVITSRFTTRQESTLPQVWIQWQ----GKPADE 116
+AV + Q L + E+++S F + S+ + L + W+ KP
Sbjct: 240 KAVA-ATANPQPLPLMLSEDLSS--EFFQLKSKLSITILMVLLKFSFNWKIFRILKPLGN 410
Query: 117 PTWKDTLNIRSQFPVFNLEDK 137
P WK + R+ FP F L +
Sbjct: 411 P-WKSS---RNSFPHFTLRTR 461
Score = 38.5 bits (88), Expect = 0.001
Identities = 19/42 (45%), Positives = 25/42 (59%)
Frame = +2
Query: 102 LPQVWIQWQGKPADEPTWKDTLNIRSQFPVFNLEDKVDLSAG 143
+ +V IQ + P E TW+ I+ QFP F+LEDKV L G
Sbjct: 353 IAEVLIQLEDLPDFEATWESVEVIKEQFPSFHLEDKVTLLGG 478
>BE807407
Length = 341
Score = 41.6 bits (96), Expect(2) = 1e-07
Identities = 16/28 (57%), Positives = 24/28 (85%)
Frame = +2
Query: 19 PQLTAPYYGPYPIIQRIGAVAYRLQLPE 46
P+LTA YYGPY ++ ++G VA++LQLP+
Sbjct: 155 PKLTARYYGPYLVLIKVGVVAFQLQLPK 238
Score = 29.6 bits (65), Expect(2) = 1e-07
Identities = 14/26 (53%), Positives = 18/26 (68%)
Frame = +1
Query: 51 HPVFHASLLKEAVGNNSVELQLLDHL 76
+PVFHA LK AVG++ VE +L L
Sbjct: 250 YPVFHALQLKRAVGHHLVEAELHQEL 327
>BU549069
Length = 615
Score = 44.3 bits (103), Expect = 2e-05
Identities = 30/113 (26%), Positives = 52/113 (45%), Gaps = 2/113 (1%)
Frame = -1
Query: 20 QLTAPYYGPYPIIQRIGAVAYRLQLPEG-GRVHPVFHASLLKEAVGNNSVELQLLDHLTG 78
+LT + G + I++R VAY++ LP +H VFH S L+ + + S ++L D
Sbjct: 567 KLTPHFIGHFQILKRAXPVAYQIALPPSLSNLHNVFHVSQLRMYIHDPSHVVKLDDVQVK 388
Query: 79 EEVA-SVHPFSVITSRFTTRQESTLPQVWIQWQGKPADEPTWKDTLNIRSQFP 130
E + P + R + P V + W G ++ TW+ +R +P
Sbjct: 387 ENLTYETLPLRIEDRRTKHLRRKENPLVKVIWGGTSGEDATWELESQMRVAYP 229
>TC204929 similar to GB|AAD39665.1|5103835|F9L1 ESTs gb|T22508, gb|H36196 and
gb|AI100134 come from this gene. {Arabidopsis thaliana;}
, partial (53%)
Length = 823
Score = 41.6 bits (96), Expect = 1e-04
Identities = 19/62 (30%), Positives = 36/62 (57%)
Frame = -2
Query: 84 VHPFSVITSRFTTRQESTLPQVWIQWQGKPADEPTWKDTLNIRSQFPVFNLEDKVDLSAG 143
VHPF+++ + + + + +V +QW+G P +E +W+ ++R + NL DKV G
Sbjct: 636 VHPFAILDWKLDSSGMTPVKRVLVQWEGLPPEEASWELWDDLRD---LHNLADKVVFDEG 466
Query: 144 GI 145
G+
Sbjct: 465 GV 460
>BQ272766 weakly similar to GP|28558781|gb| pol protein {Cucumis melo},
partial (9%)
Length = 410
Score = 35.8 bits (81), Expect = 0.008
Identities = 26/101 (25%), Positives = 47/101 (45%), Gaps = 2/101 (1%)
Frame = -3
Query: 20 QLTAPYYGPYPIIQRIGAVAYRLQLPEG-GRVHPVFHASLLKEAVGNNSVELQLLDHLTG 78
+LT + GP+ I++++ VAY++ LP +H VFH S + + + + S + L
Sbjct: 303 KLTPHFIGPFQILKKVDFVAYQIALPPSLTSLHNVFHVSQIHKYINDPSHMVNLDVVQVK 124
Query: 79 EEVA-SVHPFSVITSRFTTRQESTLPQVWIQWQGKPADEPT 118
E +A P + R + + V + W G +E T
Sbjct: 123 ENLAHETLPLRIKDMRTKHLRGKEILLVKVIWGGASGEEAT 1
>BE211654
Length = 454
Score = 33.5 bits (75), Expect = 0.038
Identities = 15/40 (37%), Positives = 24/40 (59%)
Frame = -3
Query: 105 VWIQWQGKPADEPTWKDTLNIRSQFPVFNLEDKVDLSAGG 144
V I+W+G P E +W+ +++ FP +LE+KV L G
Sbjct: 452 VLIKWRGLPHHEDSWELADEMQAVFPQLDLENKVHLEGRG 333
>AW348690
Length = 644
Score = 30.4 bits (67), Expect = 0.32
Identities = 25/70 (35%), Positives = 32/70 (45%), Gaps = 4/70 (5%)
Frame = +3
Query: 75 HLTGEEVASVHPFSVITSRFTTRQESTLPQVWIQWQGKPADEPTWKDTLNIRS----QFP 130
H T + V P SV+ + +E +V I W D P KD L + S QFP
Sbjct: 330 HSTDDMELQVSPESVLIAHSNASREV---EVLIHWH----DIPISKDFLYLFSLMQQQFP 488
Query: 131 VFNLEDKVDL 140
F+ EDKV L
Sbjct: 489 SFHFEDKVSL 518
>BU578017
Length = 425
Score = 30.0 bits (66), Expect = 0.42
Identities = 20/70 (28%), Positives = 33/70 (46%), Gaps = 2/70 (2%)
Frame = +2
Query: 49 RVHPVFHASLLKEAVGNNSVE--LQLLDHLTGEEVASVHPFSVITSRFTTRQESTLPQVW 106
R+H VFH SLLK G +L D ++ + P +++ R ++ + +V
Sbjct: 8 RIHLVFHCSLLKPFHGTPDSHNFAELPDKFINDQ-PFLTPLAILDYRRSSSAANAPWEVL 184
Query: 107 IQWQGKPADE 116
+QW G DE
Sbjct: 185 VQWHGLSPDE 214
>TC218053 weakly similar to UP|Q6I621 (Q6I621) Protein phosphatase 2A B'kappa
subunit, partial (74%)
Length = 2136
Score = 29.6 bits (65), Expect = 0.55
Identities = 18/51 (35%), Positives = 28/51 (54%), Gaps = 5/51 (9%)
Frame = -3
Query: 92 SRFTTRQESTLPQ-----VWIQWQGKPADEPTWKDTLNIRSQFPVFNLEDK 137
+RF Q + PQ +++QWQGKPA++ T K+ +R LE+K
Sbjct: 1291 NRFRFVQRNQSPQQKYLVLFLQWQGKPAND-TPKNLQQLRDSIMSLGLENK 1142
>TC211469
Length = 480
Score = 28.9 bits (63), Expect = 0.94
Identities = 14/62 (22%), Positives = 30/62 (47%)
Frame = +2
Query: 84 VHPFSVITSRFTTRQESTLPQVWIQWQGKPADEPTWKDTLNIRSQFPVFNLEDKVDLSAG 143
V P +++ + + V +QW G P ++ +W+ +++ +NLED+V
Sbjct: 71 VTPLTILDWKLDSSVTPPRRLVLVQWMGLPPEDTSWELWDDLQQS---YNLEDEVVFGGA 241
Query: 144 GI 145
G+
Sbjct: 242 GV 247
>BU547665
Length = 653
Score = 28.9 bits (63), Expect = 0.94
Identities = 11/19 (57%), Positives = 13/19 (67%)
Frame = -3
Query: 126 RSQFPVFNLEDKVDLSAGG 144
++ FP FNL DK D SA G
Sbjct: 369 KNSFPTFNLRDKADFSASG 313
>CA784736
Length = 438
Score = 28.1 bits (61), Expect = 1.6
Identities = 12/25 (48%), Positives = 17/25 (68%)
Frame = +2
Query: 116 EPTWKDTLNIRSQFPVFNLEDKVDL 140
E TW+D +I S +P NLE KV++
Sbjct: 50 EATWEDEDSIGSTYPDLNLEHKVEV 124
>BG790334 weakly similar to GP|2160154|gb| F19K23.18 gene product
{Arabidopsis thaliana}, partial (19%)
Length = 390
Score = 26.6 bits (57), Expect = 4.6
Identities = 9/20 (45%), Positives = 11/20 (55%)
Frame = +3
Query: 100 STLPQVWIQWQGKPADEPTW 119
STLP +W W GK + W
Sbjct: 144 STLPPLWTSWDGKGSFRKLW 203
>BM521595
Length = 320
Score = 25.8 bits (55), Expect = 7.9
Identities = 16/68 (23%), Positives = 33/68 (48%)
Frame = -1
Query: 58 LLKEAVGNNSVELQLLDHLTGEEVASVHPFSVITSRFTTRQESTLPQVWIQWQGKPADEP 117
L + N+++ LLD TGE V ++ SV + + L + +Q++ +++
Sbjct: 206 LASSSASRNAIKSSLLDECTGESVTTLICLSV-N*KQMDE*DGALG*ICMQFENHVSEDG 30
Query: 118 TWKDTLNI 125
W++ NI
Sbjct: 29 *WRENPNI 6
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.320 0.137 0.416
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,542,064
Number of Sequences: 63676
Number of extensions: 83850
Number of successful extensions: 396
Number of sequences better than 10.0: 44
Number of HSP's better than 10.0 without gapping: 392
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 392
length of query: 147
length of database: 12,639,632
effective HSP length: 88
effective length of query: 59
effective length of database: 7,036,144
effective search space: 415132496
effective search space used: 415132496
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 54 (25.4 bits)
Lotus: description of TM0301a.4