BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0290.16
(172 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
CA936755 96 8e-23
TC232603 weakly similar to UP|Q39482 (Q39482) Legumin (Fragment)... 37 0.006
TC233199 36 0.008
CO982668 30 0.73
TC234374 29 1.3
TC218847 29 1.3
TC217632 weakly similar to UP|Q7XYU8 (Q7XYU8) Cysteine protease,... 28 1.6
TC204494 similar to UP|O23961 (O23961) Peroxidase precursor, par... 28 2.1
AW317483 similar to SP|P10742|S25K Stem 31 kDa glycoprotein prec... 28 2.1
TC215297 similar to UP|Q40090 (Q40090) SPF1 protein, partial (33%) 28 2.8
BM085937 weakly similar to GP|14488069|gb| AT5g40270/MSN9_170 {A... 28 2.8
TC209784 UP|Q8BY04 (Q8BY04) Mus musculus 7 days neonate cerebell... 27 3.6
TC218620 similar to UP|Q8GUA8 (Q8GUA8) Ornithine aminotransferas... 27 4.8
AW832247 weakly similar to GP|22138108|gb| 40S ribosomal S4 prot... 27 6.2
CA800510 27 6.2
BU547209 26 8.1
TC231133 similar to UP|Q7X8K7 (Q7X8K7) CTR1-like kinase kinase k... 26 8.1
TC219326 UP|AXX1_SOYBN (P33082) Auxin-induced protein X15, complete 26 8.1
>CA936755
Length = 424
Score = 96.3 bits (238), Expect(2) = 8e-23
Identities = 40/92 (43%), Positives = 58/92 (62%)
Frame = -2
Query: 19 LVSNEWGTFWPNCSQLVLNRDVSDHCPVLLRQTFQDWGPKPFRVLNCWLGDHRLPALVEN 78
LVS +W WP+ SQ L RD SDHCP++++ DWGPKPFRV++ WL LV
Sbjct: 291 LVSEQWLLSWPDSSQYTLPRDFSDHCPIIMQTKKVDWGPKPFRVVDWWLHQKGYQRLVRE 112
Query: 79 SWKDMKVKGWGAFMVKEKLKGLRSVLKEWNRE 110
+W + GWG ++K KL+ L+ +K+W++E
Sbjct: 111 TWSAEQQPGWGGILLKNKLRMLKLSIKQWSKE 16
Score = 26.9 bits (58), Expect(2) = 8e-23
Identities = 10/16 (62%), Positives = 13/16 (80%)
Frame = -1
Query: 2 FTWHRPNNQAQSRLDR 17
+TW RPN + +SRLDR
Sbjct: 343 YTWIRPNARVKSRLDR 296
>TC232603 weakly similar to UP|Q39482 (Q39482) Legumin (Fragment), partial
(4%)
Length = 1123
Score = 36.6 bits (83), Expect = 0.006
Identities = 16/40 (40%), Positives = 24/40 (60%)
Frame = +2
Query: 1 KFTWHRPNNQAQSRLDRVLVSNEWGTFWPNCSQLVLNRDV 40
K+ W++ + SRLDR LV E T WPN + ++L R +
Sbjct: 551 KYNWYKDDKTTMSRLDRFLVYEE*LTTWPNMTLILLLRRI 670
>TC233199
Length = 1105
Score = 36.2 bits (82), Expect = 0.008
Identities = 16/29 (55%), Positives = 20/29 (68%)
Frame = +1
Query: 15 LDRVLVSNEWGTFWPNCSQLVLNRDVSDH 43
LDRVLVS EW + W Q +L+R+ SDH
Sbjct: 466 LDRVLVSVEWFSKWSGSIQFILDRNYSDH 552
>CO982668
Length = 870
Score = 29.6 bits (65), Expect = 0.73
Identities = 22/76 (28%), Positives = 31/76 (39%), Gaps = 3/76 (3%)
Frame = -2
Query: 8 NNQAQSRLDRVLVSNEWGTFWPNCSQLVLNRDVSDHCPVLLRQTF---QDWGPKPFRVLN 64
NN Q RLDRVL++ EW P L D P + + P+R N
Sbjct: 281 NNFFQVRLDRVLMNQEWRIESPIVKVTHLFHCK*DQ*PHHINLDVG*PMNRKRCPYRFQN 102
Query: 65 CWLGDHRLPALVENSW 80
WL +L+ ++W
Sbjct: 101 AWLSHDEFDSLIRHNW 54
>TC234374
Length = 787
Score = 28.9 bits (63), Expect = 1.3
Identities = 19/85 (22%), Positives = 38/85 (44%)
Frame = +2
Query: 85 VKGWGAFMVKEKLKGLRSVLKEWNREVFGDLRSRREVVVTQINNLDLKEEEVGLDQAEVD 144
V G ++++KLK + LK W + VFG++ +++++ + VG A +
Sbjct: 50 VLGCPIVVLQQKLKM*KC*LKTWIKNVFGNVHQLVL*ATNEVDSIQQQIVNVGYSDALFN 229
Query: 145 LRKALFNDFWAILRLHESFLCQKAR 169
+ +LR E +K+R
Sbjct: 230 QEVLAQKELQTVLRFQECLWREKSR 304
>TC218847
Length = 521
Score = 28.9 bits (63), Expect = 1.3
Identities = 11/19 (57%), Positives = 13/19 (67%)
Frame = -3
Query: 154 WAILRLHESFLCQKARSKW 172
W + HES L QKAR+KW
Sbjct: 282 WTAAQAHESLLRQKARTKW 226
>TC217632 weakly similar to UP|Q7XYU8 (Q7XYU8) Cysteine protease, partial
(24%)
Length = 844
Score = 28.5 bits (62), Expect = 1.6
Identities = 12/30 (40%), Positives = 16/30 (53%)
Frame = +2
Query: 16 DRVLVSNEWGTFWPNCSQLVLNRDVSDHCP 45
D +V N WG W L++ R+VSD P
Sbjct: 452 DYWIVKNSWGKDWGEEGYLLIKRNVSDEWP 541
>TC204494 similar to UP|O23961 (O23961) Peroxidase precursor, partial (93%)
Length = 1326
Score = 28.1 bits (61), Expect = 2.1
Identities = 15/37 (40%), Positives = 21/37 (56%)
Frame = -3
Query: 13 SRLDRVLVSNEWGTFWPNCSQLVLNRDVSDHCPVLLR 49
S+L R+L WG W NC + V++R V PV L+
Sbjct: 811 SKLTRLLGGPPWGHIWRNCFK*VVSR-VGSGLPVPLK 704
>AW317483 similar to SP|P10742|S25K Stem 31 kDa glycoprotein precursor
(Vegetative storage protein VSP25) (Fragment).
[Soybean], partial (50%)
Length = 451
Score = 28.1 bits (61), Expect = 2.1
Identities = 15/42 (35%), Positives = 18/42 (42%)
Frame = +3
Query: 24 WGTFWPNCSQLVLNRDVSDHCPVLLRQTFQDWGPKPFRVLNC 65
W C L R + HCP LLR T WG R+ +C
Sbjct: 78 WRNLTRPCMMNGLTRAMHRHCPTLLRIT-TSWGLMASRLYSC 200
>TC215297 similar to UP|Q40090 (Q40090) SPF1 protein, partial (33%)
Length = 1298
Score = 27.7 bits (60), Expect = 2.8
Identities = 12/30 (40%), Positives = 20/30 (66%)
Frame = -1
Query: 34 LVLNRDVSDHCPVLLRQTFQDWGPKPFRVL 63
L++ D + H P++L +FQ+W P PF V+
Sbjct: 266 LLI*YDQNHHLPLIL-MSFQEWQPNPFDVV 180
>BM085937 weakly similar to GP|14488069|gb| AT5g40270/MSN9_170 {Arabidopsis
thaliana}, partial (18%)
Length = 428
Score = 27.7 bits (60), Expect = 2.8
Identities = 11/38 (28%), Positives = 23/38 (59%)
Frame = +2
Query: 119 REVVVTQINNLDLKEEEVGLDQAEVDLRKALFNDFWAI 156
+++V +Q N +LKEE+V + ++DL + N ++
Sbjct: 296 QDIVCSQTNGANLKEEDVVVSYVKIDLTRGRANPLGSV 409
>TC209784 UP|Q8BY04 (Q8BY04) Mus musculus 7 days neonate cerebellum cDNA,
RIKEN full-length enriched library, clone:A730012G10
product:CEREBELLUM POSTNATAL DEVELOPMENT ASSOCIATED
PROTEIN 2, full insert sequence, partial (6%)
Length = 1004
Score = 27.3 bits (59), Expect = 3.6
Identities = 13/43 (30%), Positives = 20/43 (46%)
Frame = -2
Query: 3 TWHRPNNQAQSRLDRVLVSNEWGTFWPNCSQLVLNRDVSDHCP 45
T H N Q ++ ++S WG P C +L +R + H P
Sbjct: 283 TTHCINRQCKAISGNDIISLRWGQVVPKCLKLPSSRPIP*HTP 155
>TC218620 similar to UP|Q8GUA8 (Q8GUA8) Ornithine aminotransferase , partial
(49%)
Length = 960
Score = 26.9 bits (58), Expect = 4.8
Identities = 10/21 (47%), Positives = 12/21 (56%)
Frame = -2
Query: 48 LRQTFQDWGPKPFRVLNCWLG 68
L QT+ WG K + CWLG
Sbjct: 569 LIQTYAKWGSKADNCIMCWLG 507
>AW832247 weakly similar to GP|22138108|gb| 40S ribosomal S4 protein {Glycine
max}, partial (35%)
Length = 283
Score = 26.6 bits (57), Expect = 6.2
Identities = 10/20 (50%), Positives = 11/20 (55%)
Frame = -2
Query: 28 WPNCSQLVLNRDVSDHCPVL 47
WP CS L R + HCP L
Sbjct: 117 WPGCSVLARPRSHAFHCPAL 58
>CA800510
Length = 409
Score = 26.6 bits (57), Expect = 6.2
Identities = 12/27 (44%), Positives = 13/27 (47%)
Frame = -2
Query: 146 RKALFNDFWAILRLHESFLCQKARSKW 172
R +FWA R ES L QK KW
Sbjct: 189 RSVFQQEFWAFTRQKESSLFQKWHPKW 109
>BU547209
Length = 627
Score = 26.2 bits (56), Expect = 8.1
Identities = 11/29 (37%), Positives = 14/29 (47%)
Frame = +1
Query: 3 TWHRPNNQAQSRLDRVLVSNEWGTFWPNC 31
TW+ PN + L+S EWG F C
Sbjct: 316 TWYSPN**VSESIFSQLLSQEWGYFLEYC 402
>TC231133 similar to UP|Q7X8K7 (Q7X8K7) CTR1-like kinase kinase kinase,
partial (29%)
Length = 1061
Score = 26.2 bits (56), Expect = 8.1
Identities = 13/38 (34%), Positives = 22/38 (57%), Gaps = 2/38 (5%)
Frame = +1
Query: 5 HRPNNQ--AQSRLDRVLVSNEWGTFWPNCSQLVLNRDV 40
HRPNNQ + RL L + + NC+ ++++RD+
Sbjct: 286 HRPNNQLDERRRLRMALDAARGMNYLHNCTPVIVHRDL 399
>TC219326 UP|AXX1_SOYBN (P33082) Auxin-induced protein X15, complete
Length = 487
Score = 26.2 bits (56), Expect = 8.1
Identities = 22/74 (29%), Positives = 33/74 (43%), Gaps = 10/74 (13%)
Frame = +3
Query: 57 PKPFRVLNCWLGDH----------RLPALVENSWKDMKVKGWGAFMVKEKLKGLRSVLKE 106
PK F++LN +L RLP + + S KG+ A V EK+K +
Sbjct: 15 PKAFKILNTFLKKFSSQINTTMGFRLPGIRKASNAVDAPKGYLAVYVGEKMKRFVIPVSY 194
Query: 107 WNREVFGDLRSRRE 120
N+ F DL ++ E
Sbjct: 195 MNQPSFQDLLTQAE 236
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.323 0.138 0.460
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,508,597
Number of Sequences: 63676
Number of extensions: 135559
Number of successful extensions: 645
Number of sequences better than 10.0: 36
Number of HSP's better than 10.0 without gapping: 643
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 645
length of query: 172
length of database: 12,639,632
effective HSP length: 91
effective length of query: 81
effective length of database: 6,845,116
effective search space: 554454396
effective search space used: 554454396
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 55 (25.8 bits)
Lotus: description of TM0290.16