BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0276.3
(333 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC228718 similar to UP|PIP_ARATH (P93732) Proline iminopeptidase... 324 4e-89
TC228717 similar to GB|AAC49560.1|1710151|ATU72711 proline imino... 290 8e-79
BI471614 92 2e-19
TC230419 weakly similar to UP|Q9ZP87 (Q9ZP87) Epoxide hydrolase,... 47 9e-06
BI973866 weakly similar to GP|1354849|gb| epoxide hydrolase {Nic... 47 9e-06
TC221106 similar to UP|Q9ZP87 (Q9ZP87) Epoxide hydrolase, partia... 46 3e-05
TC220763 weakly similar to UP|Q9ZP87 (Q9ZP87) Epoxide hydrolase,... 37 0.012
TC207323 35 0.036
TC216423 UP|O49857 (O49857) Epoxide hydrolase, complete 35 0.062
TC224170 35 0.062
TC215081 weakly similar to UP|DAN4_YEAST (P47179) Cell wall prot... 34 0.11
TC225312 similar to UP|Q76E11 (Q76E11) Soluble epoxide hydrolase... 34 0.11
TC209233 similar to UP|Q39856 (Q39856) Epoxide hydrolase, partia... 33 0.18
TC225313 similar to UP|Q39856 (Q39856) Epoxide hydrolase, partia... 33 0.18
TC233337 similar to GB|AAO63446.1|28951045|BT005382 At1g64670 {A... 33 0.23
TC214636 similar to UP|Q39856 (Q39856) Epoxide hydrolase, partia... 32 0.52
AW755775 similar to PIR|T07145|T07 epoxide hydrolase homolog - s... 32 0.52
TC234020 similar to UP|Q9M314 (Q9M314) Prolyl aminopeptidase-lik... 31 0.68
TC223586 31 0.68
TC214655 similar to UP|Q39856 (Q39856) Epoxide hydrolase, partia... 30 1.2
>TC228718 similar to UP|PIP_ARATH (P93732) Proline iminopeptidase (PIP)
(Prolyl aminopeptidase) (PAP) , partial (47%)
Length = 757
Score = 324 bits (830), Expect = 4e-89
Identities = 150/165 (90%), Positives = 159/165 (95%)
Frame = +2
Query: 166 PDAWEPFRDLIPENERGCFVDAYKKRLNSDDIQTQFAAARAWTKWEMMTAHLIPNEDNVK 225
PDAWEPFRDLIPE+ERGCFVDAY KRLNSDDI+TQ+AAARAWTKWEMMTAHL+PNE+N+K
Sbjct: 2 PDAWEPFRDLIPESERGCFVDAYNKRLNSDDIKTQYAAARAWTKWEMMTAHLLPNEENIK 181
Query: 226 KGDDDHFSLAFARIENHYFVNKGFFSSDSYLLDGVDKIRHINTTIVQGRYDVCCPMMSAW 285
KGDDD+FSLAFARIENHYFVNKGFF SDS+LLD +DKIRHINTTIVQGRYDVCCPMMSAW
Sbjct: 182 KGDDDYFSLAFARIENHYFVNKGFFPSDSFLLDRIDKIRHINTTIVQGRYDVCCPMMSAW 361
Query: 286 DLHKAWPEADFRVVADAGHSANELGIAAELVAANEKLKNIIKNKG 330
DLHKAWPEADFRVV DAGHSANE GI AELVAANEKLKNIIKNKG
Sbjct: 362 DLHKAWPEADFRVVPDAGHSANEPGITAELVAANEKLKNIIKNKG 496
>TC228717 similar to GB|AAC49560.1|1710151|ATU72711 proline iminopeptidase
{Arabidopsis thaliana;} , partial (43%)
Length = 629
Score = 290 bits (741), Expect = 8e-79
Identities = 134/153 (87%), Positives = 144/153 (93%)
Frame = +3
Query: 1 MASEQEFSELNRNLYPHIEPYSTGLLKVSDLHTIYWEQSGNPSGHPVVFLHGGPGGGTSP 60
MASE EFSELNRNLYP IEPYSTG LKVSDLHTIYWEQSGNPSGHPVVF+HGGPGGGTSP
Sbjct: 171 MASENEFSELNRNLYPQIEPYSTGFLKVSDLHTIYWEQSGNPSGHPVVFIHGGPGGGTSP 350
Query: 61 SNRRFFDPEFYRIILFDQRGAGKSTPHACLEHNTTWDLIDDIEKLREHLEIPEWQVFGGS 120
SNR+FFDP+FYRI+LFDQR AGKSTPHACLE NTTWDLIDDI+KLREHLEI +WQVFGGS
Sbjct: 351 SNRKFFDPDFYRIVLFDQRSAGKSTPHACLEQNTTWDLIDDIDKLREHLEIADWQVFGGS 530
Query: 121 WGSTLALAYSQSHPDKVTGIILRGIFLLRKKEI 153
WGSTLALAY QS+ DKVTG+ILR +FLLRKKE+
Sbjct: 531 WGSTLALAYRQSYHDKVTGMILRRMFLLRKKEM 629
>BI471614
Length = 424
Score = 92.4 bits (228), Expect = 2e-19
Identities = 42/45 (93%), Positives = 42/45 (93%)
Frame = +2
Query: 1 MASEQEFSELNRNLYPHIEPYSTGLLKVSDLHTIYWEQSGNPSGH 45
MASE EFSELNRNLYP IEPYSTG LKVSDLHTIYWEQSGNPSGH
Sbjct: 134 MASENEFSELNRNLYPQIEPYSTGFLKVSDLHTIYWEQSGNPSGH 268
>TC230419 weakly similar to UP|Q9ZP87 (Q9ZP87) Epoxide hydrolase, partial
(35%)
Length = 536
Score = 47.4 bits (111), Expect = 9e-06
Identities = 34/100 (34%), Positives = 49/100 (49%), Gaps = 1/100 (1%)
Frame = +2
Query: 47 VVFLHGGPGGGTSPSNRRFFDPEF-YRIILFDQRGAGKSTPHACLEHNTTWDLIDDIEKL 105
VVFLHG P + ++ YR I FD RG G S A E T +DL+D+I L
Sbjct: 191 VVFLHGFPEIWYTWRHQMIAAANAGYRAIAFDFRGYGLSEHPAEPEKETMYDLVDEIVGL 370
Query: 106 REHLEIPEWQVFGGSWGSTLALAYSQSHPDKVTGIILRGI 145
+ L I + + G +G+ + HP++V +I GI
Sbjct: 371 LDALNITQAFLVGKDFGAIPGYLTAAVHPERVAAVITLGI 490
>BI973866 weakly similar to GP|1354849|gb| epoxide hydrolase {Nicotiana
tabacum}, partial (33%)
Length = 420
Score = 47.4 bits (111), Expect = 9e-06
Identities = 31/103 (30%), Positives = 53/103 (51%), Gaps = 1/103 (0%)
Frame = +1
Query: 44 GHPVVFLHGGPGGGTSPSNRRF-FDPEFYRIILFDQRGAGKSTPHACLEHNTTWDLIDDI 102
G+ VVFLHG P S ++ +R + FD RG G S P T +DL++D+
Sbjct: 85 GNAVVFLHGFPEIWYSWRHQMIALADAGFRAVSFDYRGYGLSDPPPPGNKATWFDLLNDL 264
Query: 103 EKLREHLEIPEWQVFGGSWGSTLALAYSQSHPDKVTGIILRGI 145
+ + L + + + G +G+ A +S HP++V G++ G+
Sbjct: 265 LHILDALALSKVFLVGKDFGARPAHFFSILHPERVLGVVTLGV 393
>TC221106 similar to UP|Q9ZP87 (Q9ZP87) Epoxide hydrolase, partial (31%)
Length = 585
Score = 45.8 bits (107), Expect = 3e-05
Identities = 35/100 (35%), Positives = 49/100 (49%), Gaps = 1/100 (1%)
Frame = +1
Query: 47 VVFLHGGPGGGTSPSNRRFFDPEF-YRIILFDQRGAGKSTPHACLEHNTTWDLIDDIEKL 105
VVFLHG P + ++ YR I FD RG G S A E T +DL+ +I L
Sbjct: 94 VVFLHGFPEIWYTWRHQMISVANAGYRAIAFDFRGYGLSQQPAEPEKETMFDLVHEIVGL 273
Query: 106 REHLEIPEWQVFGGSWGSTLALAYSQSHPDKVTGIILRGI 145
+ L I + + G +G+ + HP++V GII GI
Sbjct: 274 LDALNISKAFLVGKDFGAIPGHLTTAVHPERVAGIITLGI 393
>TC220763 weakly similar to UP|Q9ZP87 (Q9ZP87) Epoxide hydrolase, partial
(75%)
Length = 992
Score = 37.0 bits (84), Expect = 0.012
Identities = 22/66 (33%), Positives = 33/66 (49%)
Frame = +1
Query: 80 GAGKSTPHACLEHNTTWDLIDDIEKLREHLEIPEWQVFGGSWGSTLALAYSQSHPDKVTG 139
G G S A E DL+DD+ L + L I + + G +G+ A + HPDKV
Sbjct: 1 GYGLSEHPAEPEKANLLDLVDDVVGLLDSLSITKAVLVGKDFGAFPAYIVAALHPDKVDS 180
Query: 140 IILRGI 145
+I+ G+
Sbjct: 181 VIMLGV 198
>TC207323
Length = 1046
Score = 35.4 bits (80), Expect = 0.036
Identities = 31/104 (29%), Positives = 48/104 (45%), Gaps = 5/104 (4%)
Frame = +3
Query: 44 GHPVVFLHGGPGGGTSPSNRRFFDPEF---YRIILFDQRGAGKSTP-HACLEHNTTWD-L 98
G+ +V L G GT S + P Y++IL+D GAG + P + E T D
Sbjct: 135 GNEIVIL--AHGFGTDQSVWKHLVPHLVDDYQVILYDNMGAGTTNPDYFDFERYYTIDGF 308
Query: 99 IDDIEKLREHLEIPEWQVFGGSWGSTLALAYSQSHPDKVTGIIL 142
+ D+ + + L++ G S + + L S SHP T +IL
Sbjct: 309 VYDLLAILQELQVQSCIFVGHSLSAMVGLLASISHPHLFTKLIL 440
>TC216423 UP|O49857 (O49857) Epoxide hydrolase, complete
Length = 1343
Score = 34.7 bits (78), Expect = 0.062
Identities = 26/108 (24%), Positives = 45/108 (41%), Gaps = 1/108 (0%)
Frame = +1
Query: 44 GHPVVFLHGGPGGGTSPSNRRFFDPEF-YRIILFDQRGAGKSTPHACLEHNTTWDLIDDI 102
G V+FLHG P S ++ YR + D RG G + + + ++ D+
Sbjct: 217 GPVVLFLHGFPELWYSWRHQILSLSSLGYRAVAPDLRGYGDTEAPPSISSYNCFHIVGDL 396
Query: 103 EKLREHLEIPEWQVFGGSWGSTLALAYSQSHPDKVTGIILRGIFLLRK 150
L + L + + + WG+ + PDKV + + LLR+
Sbjct: 397 VALIDSLGVQQVFLVAHDWGAIIGWYLCMFRPDKVKAYVCLSVPLLRR 540
>TC224170
Length = 518
Score = 34.7 bits (78), Expect = 0.062
Identities = 29/106 (27%), Positives = 45/106 (42%), Gaps = 5/106 (4%)
Frame = +1
Query: 42 PSGHPVVFLHGGPGGGTSPSNRRFFDP---EFYRIILFDQRGAGKSTP--HACLEHNTTW 96
P +V HG GT S + P + YR+IL+D GAG + P H++
Sbjct: 154 PGTECIVLAHGF---GTDQSVWKHLVPYLVDDYRVILYDNMGAGTTNPDYFDFERHSSLE 324
Query: 97 DLIDDIEKLREHLEIPEWQVFGGSWGSTLALAYSQSHPDKVTGIIL 142
D+ + E L++ G S + + S S PD T +I+
Sbjct: 325 GYASDLLAILEELQVESCIFVGHSVSAMIGAIASISRPDLFTKLIM 462
>TC215081 weakly similar to UP|DAN4_YEAST (P47179) Cell wall protein DAN4
precursor, partial (3%)
Length = 597
Score = 33.9 bits (76), Expect = 0.11
Identities = 34/112 (30%), Positives = 48/112 (42%), Gaps = 6/112 (5%)
Frame = +2
Query: 55 GGGTSPSNRRFFDPEF---YRIILFDQRGAGKSTP-HACLEHNTTWDL-IDDIEKLREHL 109
G GT S + P F Y +IL+D AG P H TT D +DD+ + + L
Sbjct: 218 GFGTDQSAWQRVLPYFTRNYSVILYDLVCAGSVNPDHFDYRRYTTLDAYVDDLLNILDAL 397
Query: 110 EIPEWQVFGGSWGSTLALAYSQSHPDKVTGIILRGIFLLRKKEI-DWFYEGG 160
+P G S + + + S PD + +IL G K + D Y GG
Sbjct: 398 RVPRCAYVGHSISAMIGMLASIRRPDLFSKLILIGASPRYNKFLNDKDYHGG 553
>TC225312 similar to UP|Q76E11 (Q76E11) Soluble epoxide hydrolase, partial
(96%)
Length = 1213
Score = 33.9 bits (76), Expect = 0.11
Identities = 26/100 (26%), Positives = 44/100 (44%), Gaps = 1/100 (1%)
Frame = +2
Query: 43 SGHPVVFLHGGPGGGTSPSNRRF-FDPEFYRIILFDQRGAGKSTPHACLEHNTTWDLIDD 101
SG V+FLHG P + ++ YR I D RG G + + ++ D
Sbjct: 107 SGPVVLFLHGFPELWYTWRHQLLSLSAVGYRAIAPDLRGYGDTDAPPDASSYSALHIVAD 286
Query: 102 IEKLREHLEIPEWQVFGGSWGSTLALAYSQSHPDKVTGII 141
+ L + L I + G WG+++A + PD+V ++
Sbjct: 287 LVGLLDALGIERVFLVGHDWGASIAWHFCLLRPDRVKALV 406
>TC209233 similar to UP|Q39856 (Q39856) Epoxide hydrolase, partial (53%)
Length = 590
Score = 33.1 bits (74), Expect = 0.18
Identities = 30/122 (24%), Positives = 53/122 (42%), Gaps = 8/122 (6%)
Frame = +1
Query: 44 GHPVVFLHGGPGGGTSPSNRRFFDPEF-YRIILFDQRGAGKSTPHACLEHNTTWDLIDDI 102
G V+FLHG P S ++ YR + D RG G + + + T + L+ DI
Sbjct: 112 GAVVLFLHGFPELWYSWRHQILALSSLGYRAVAPDLRGYGDTEAPSTVNSYTCFHLVGDI 291
Query: 103 EKLREHLEIPEWQVFGGSWGSTLALAYSQSHPDKVTGIILRGI----FLLR---KKEIDW 155
L + L + + + WG+ + PD++ + + FL R +K +D+
Sbjct: 292 IALIDSLGVDKVFLVAHDWGAIIGWYLCLFRPDRIKAYVCLSVPFRPFLGRNPKQKTVDF 471
Query: 156 FY 157
F+
Sbjct: 472 FH 477
>TC225313 similar to UP|Q39856 (Q39856) Epoxide hydrolase, partial (51%)
Length = 1307
Score = 33.1 bits (74), Expect = 0.18
Identities = 27/112 (24%), Positives = 46/112 (40%), Gaps = 2/112 (1%)
Frame = +3
Query: 44 GHPVVFLHGGPGGGTSPSNRRFFDPEF-YRIILFDQRGAGKSTPHACLEHNTTWDLIDDI 102
G V+FLHG P S ++ YR + D RG G + A + T L+ DI
Sbjct: 696 GPVVLFLHGFPELWYSWRHQILALSNLGYRAVAPDLRGYGDTEAPASISSYTILHLVSDI 875
Query: 103 EKLREHLEIPEWQVFGGSWGSTLALAYSQSHPDKVTGIILRGI-FLLRKKEI 153
L L + + + WG+ + PD++ + + F+ R ++
Sbjct: 876 VALIHSLAVDQVFLVAHDWGAVIGWYLCLFRPDRIKAYVCLSVPFMPRNPKV 1031
>TC233337 similar to GB|AAO63446.1|28951045|BT005382 At1g64670 {Arabidopsis
thaliana;} , partial (42%)
Length = 658
Score = 32.7 bits (73), Expect = 0.23
Identities = 30/104 (28%), Positives = 47/104 (44%), Gaps = 4/104 (3%)
Frame = +2
Query: 43 SGHPVVFLHGGPGGGTSPSNRRF--FDPEFYRIILFDQRGAGKST-PHACLEHNTTWDLI 99
+G VVF+HG S F YR+ D G G+S P++ L T + +
Sbjct: 92 AGEDVVFIHGFISSSLFWSETVFPNLSKSCYRLFAVDLLGFGRSPKPNSSLY--TLREHL 265
Query: 100 DDIEK-LREHLEIPEWQVFGGSWGSTLALAYSQSHPDKVTGIIL 142
+ IE+ + E ++ + + S G LALA + HP V + L
Sbjct: 266 EMIERSVLEAHKVKSFHIVAHSLGCILALALAVKHPQSVKSLTL 397
>TC214636 similar to UP|Q39856 (Q39856) Epoxide hydrolase, partial (58%)
Length = 778
Score = 31.6 bits (70), Expect = 0.52
Identities = 23/99 (23%), Positives = 42/99 (42%), Gaps = 1/99 (1%)
Frame = +3
Query: 44 GHPVVFLHGGPGGGTSPSNRRFFDPEF-YRIILFDQRGAGKSTPHACLEHNTTWDLIDDI 102
G V+FLHG P S ++ YR + D RG G + ++ T + ++ D+
Sbjct: 129 GPVVLFLHGFPELWYSWRHQILSLSSLGYRAVAPDLRGYGDTEAPPSIDSYTCFHIVGDL 308
Query: 103 EKLREHLEIPEWQVFGGSWGSTLALAYSQSHPDKVTGII 141
L + L + + + WG+ + P+KV +
Sbjct: 309 VALIDSLGVQQVFLVAHDWGALMGWYLCMFRPEKVKAYV 425
>AW755775 similar to PIR|T07145|T07 epoxide hydrolase homolog - soybean,
partial (41%)
Length = 469
Score = 31.6 bits (70), Expect = 0.52
Identities = 30/125 (24%), Positives = 53/125 (42%), Gaps = 2/125 (1%)
Frame = +2
Query: 27 KVSDLHTIYWEQSGNPSGHPVVFLHGGPGGGTSPSNRRF-FDPEFYRIILFDQRGAGKST 85
K +++ I + G V+FLHG P S ++ F+ +R + + RG +
Sbjct: 17 KTVEVNGIKMHVAEKGEGPVVLFLHGFPELWYSWRHQILAFNNLGFRAVASELRGFRDTE 196
Query: 86 PHACLEHNTTWDLIDDIEKLREHLEIPEWQVFGGSWGSTLALAYSQSHPDKVTG-IILRG 144
A + T LI DI L L + + + WG+ + PD++ + L+G
Sbjct: 197 APASISSYTILHLISDIGALIHSLAVDQVFLVTHDWGAGIGWYLCLFRPDRIKAYVCLKG 376
Query: 145 IFLLR 149
F+ R
Sbjct: 377 PFMPR 391
>TC234020 similar to UP|Q9M314 (Q9M314) Prolyl aminopeptidase-like protein
(AT3g61540/F2A19_140), partial (22%)
Length = 436
Score = 31.2 bits (69), Expect = 0.68
Identities = 19/44 (43%), Positives = 24/44 (54%), Gaps = 4/44 (9%)
Frame = +3
Query: 47 VVFLHGGPG----GGTSPSNRRFFDPEFYRIILFDQRGAGKSTP 86
+++L GGPG T S E +R+IL DQRG G STP
Sbjct: 258 LLYLQGGPGFECRRPTESSGWTKKVCEEFRLILMDQRGTGLSTP 389
>TC223586
Length = 874
Score = 31.2 bits (69), Expect = 0.68
Identities = 32/144 (22%), Positives = 61/144 (42%), Gaps = 9/144 (6%)
Frame = +1
Query: 167 DAWEPFRDLIPENERGCFVDAYKKRLNSDDIQTQFAAARAWTKWEMMTAHLIPNEDNVKK 226
DAW L+ + + D+ + RL+SD +Q F + T+ + T + D V +
Sbjct: 61 DAWFSCMKLLEDED-----DSVRLRLSSD-VQKCFTTEK--TRSNLTTGSVPIQVDRVIR 216
Query: 227 GDDDHFSLAFARIENHY-FVNKGFFSSDSYLLDGVDKIRHINTTIVQGRYD-------VC 278
DH S F +++ ++ + ++S + D +R + + Y+ +C
Sbjct: 217 FCFDHLSSIFGHWIDYFDYLCQWVLRAESCVAPQGDLVRRVFDKEIDNHYEEKLLISQIC 396
Query: 279 CPMMSAWDLHKAWPEAD-FRVVAD 301
C M + K+W + D FR D
Sbjct: 397 CSNMEKLPILKSWADKDEFRSYLD 468
>TC214655 similar to UP|Q39856 (Q39856) Epoxide hydrolase, partial (90%)
Length = 1201
Score = 30.4 bits (67), Expect = 1.2
Identities = 24/108 (22%), Positives = 43/108 (39%), Gaps = 1/108 (0%)
Frame = +1
Query: 44 GHPVVFLHGGPGGGTSPSNRRFFDPEF-YRIILFDQRGAGKSTPHACLEHNTTWDLIDDI 102
G V+FLHG P N+ Y + D RG G + ++ T + ++ D+
Sbjct: 76 GPVVLFLHGFPELWHCWHNQIVALGSLGYHAVAPDLRGYGDTDAPPSIDSYTCFHIVADL 255
Query: 103 EKLREHLEIPEWQVFGGSWGSTLALAYSQSHPDKVTGIILRGIFLLRK 150
L + L + + WG+ + PDKV + + +R+
Sbjct: 256 VALIDSLGAEQVFLVAHDWGAIIGWYLCMFRPDKVKAYVCLSVPFIRR 399
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.320 0.138 0.439
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,357,389
Number of Sequences: 63676
Number of extensions: 241705
Number of successful extensions: 1009
Number of sequences better than 10.0: 49
Number of HSP's better than 10.0 without gapping: 1005
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1009
length of query: 333
length of database: 12,639,632
effective HSP length: 98
effective length of query: 235
effective length of database: 6,399,384
effective search space: 1503855240
effective search space used: 1503855240
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 59 (27.3 bits)
Lotus: description of TM0276.3