BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0269a.4
(70 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC215703 homologue to PIR|G96755|G96755 developmental protein ho... 91 9e-20
AW568166 similar to PIR|G96755|G967 developmental protein homolo... 54 2e-08
TC226460 similar to UP|Q9LQY6 (Q9LQY6) T24P13.4, partial (24%) 33 0.028
TC223844 similar to UP|Q84VY0 (Q84VY0) At1g70550, partial (30%) 31 0.11
TC214683 similar to UP|Q9M3Z0 (Q9M3Z0) 60S ribosomal protein L6,... 27 2.6
TC229923 weakly similar to UP|Q93Z11 (Q93Z11) AT5g37360/MNJ8_150... 27 2.6
CD416792 26 4.5
TC227832 similar to UP|TBCA_ARATH (O04350) Tubulin-specific chap... 25 5.8
CD408896 similar to GP|4164473|gb|A negatively light-regulated p... 25 5.8
TC223817 25 5.8
TC226623 similar to UP|Q9XH47 (Q9XH47) Small zinc finger-like pr... 25 7.6
CD416038 25 7.6
TC204491 homologue to UP|GCST_PEA (P49364) Aminomethyltransferas... 25 7.6
TC225583 weakly similar to UP|O94285 (O94285) Rpc25 protein (Spb... 25 9.9
TC208001 similar to UP|Q84LW7 (Q84LW7) Peptidylprolyl cis-trans ... 25 9.9
>TC215703 homologue to PIR|G96755|G96755 developmental protein homolog DG1118
[imported] - Arabidopsis thaliana {Arabidopsis
thaliana;} , complete
Length = 896
Score = 91.3 bits (225), Expect = 9e-20
Identities = 46/69 (66%), Positives = 51/69 (73%)
Frame = +2
Query: 1 MNRIMELEFTSKPLQEKPRKCEKQEKLEKLKVKSTTEKGNMGNIHIYDENTIRKHTEQMN 60
MN+IMEL+FTSK LQ + RKCEK+EK EKLKVK EKGNM IY EN IRK TEQMN
Sbjct: 110 MNQIMELKFTSKSLQRQSRKCEKEEKSEKLKVKKAIEKGNMDGARIYAENAIRKRTEQMN 289
Query: 61 YLHPSLYLD 69
YL + LD
Sbjct: 290 YLRLASRLD 316
>AW568166 similar to PIR|G96755|G967 developmental protein homolog DG1118
[imported] - Arabidopsis thaliana, partial (40%)
Length = 256
Score = 53.9 bits (128), Expect = 2e-08
Identities = 29/56 (51%), Positives = 33/56 (58%)
Frame = +2
Query: 7 LEFTSKPLQEKPRKCEKQEKLEKLKVKSTTEKGNMGNIHIYDENTIRKHTEQMNYL 62
L T K L RK +K++K EKL VK +K NM IY EN IR TEQMNYL
Sbjct: 2 LNVTLKSL*RLSRKGKKEDKSEKLNVKKANKKCNMDGARIYAENAIRNRTEQMNYL 169
>TC226460 similar to UP|Q9LQY6 (Q9LQY6) T24P13.4, partial (24%)
Length = 1129
Score = 33.1 bits (74), Expect = 0.028
Identities = 16/38 (42%), Positives = 22/38 (57%)
Frame = +1
Query: 18 PRKCEKQEKLEKLKVKSTTEKGNMGNIHIYDENTIRKH 55
P + + Q LEKLK+K TTEKG + +Y T+ H
Sbjct: 958 PERRKIQISLEKLKLKETTEKGQCSS*RVY*HPTLSPH 1071
>TC223844 similar to UP|Q84VY0 (Q84VY0) At1g70550, partial (30%)
Length = 596
Score = 31.2 bits (69), Expect = 0.11
Identities = 22/58 (37%), Positives = 32/58 (54%), Gaps = 1/58 (1%)
Frame = -1
Query: 12 KPLQEKPRKCE-KQEKLEKLKVKSTTEKGNMGNIHIYDENTIRKHTEQMNYLHPSLYL 68
KP+ KP+ + KQE+ +KL ST +G HI +TI K N ++PSLY+
Sbjct: 476 KPMSPKPKNAQKKQEEKKKLLYNSTP---*LGTSHISSRSTIVK-VIPPNIINPSLYV 315
>TC214683 similar to UP|Q9M3Z0 (Q9M3Z0) 60S ribosomal protein L6, partial
(92%)
Length = 972
Score = 26.6 bits (57), Expect = 2.6
Identities = 13/42 (30%), Positives = 23/42 (53%), Gaps = 7/42 (16%)
Frame = -1
Query: 27 LEKLKVKSTTEKGNMGNIHIYDENTIR-------KHTEQMNY 61
+E K K+T +K + N+H +DE T++ K E+ N+
Sbjct: 873 IELQKNKTTRKKKLLNNVHYHDEKTVQLLGTSSTKQNEENNF 748
>TC229923 weakly similar to UP|Q93Z11 (Q93Z11) AT5g37360/MNJ8_150, partial
(39%)
Length = 707
Score = 26.6 bits (57), Expect = 2.6
Identities = 12/42 (28%), Positives = 25/42 (58%)
Frame = +3
Query: 3 RIMELEFTSKPLQEKPRKCEKQEKLEKLKVKSTTEKGNMGNI 44
R++ TS + E+P++ + K +L+V+ T +K N+G +
Sbjct: 84 RVLTASSTSAVIVEEPKEEARVLKFGELQVEVTPDKANIGAV 209
>CD416792
Length = 579
Score = 25.8 bits (55), Expect = 4.5
Identities = 12/24 (50%), Positives = 18/24 (75%)
Frame = -3
Query: 5 MELEFTSKPLQEKPRKCEKQEKLE 28
MELEF K ++K +K +K++KLE
Sbjct: 376 MELEF*KKKKKKKKKKKKKKKKLE 305
>TC227832 similar to UP|TBCA_ARATH (O04350) Tubulin-specific chaperone A
(Tubulin-folding cofactor A) (CFA) (TCP1-chaperonin
cofactor A homolog), partial (96%)
Length = 785
Score = 25.4 bits (54), Expect = 5.8
Identities = 11/39 (28%), Positives = 22/39 (56%)
Frame = +3
Query: 16 EKPRKCEKQEKLEKLKVKSTTEKGNMGNIHIYDENTIRK 54
E+ R+C + LK+K++T K + +H Y++ R+
Sbjct: 81 ERYRRCIMATTVRSLKIKTSTCKRIVKELHSYEKEVERE 197
>CD408896 similar to GP|4164473|gb|A negatively light-regulated protein
{Vernicia fordii}, partial (15%)
Length = 608
Score = 25.4 bits (54), Expect = 5.8
Identities = 13/37 (35%), Positives = 19/37 (51%), Gaps = 2/37 (5%)
Frame = +3
Query: 29 KLKVKSTTEKGNMG--NIHIYDENTIRKHTEQMNYLH 63
KLKVKS T G+ G H Y ++ H++ + H
Sbjct: 219 KLKVKSITTNGSSGPEQYHFYHHQLLQWHSDALLMNH 329
>TC223817
Length = 731
Score = 25.4 bits (54), Expect = 5.8
Identities = 16/47 (34%), Positives = 22/47 (46%)
Frame = -1
Query: 19 RKCEKQEKLEKLKVKSTTEKGNMGNIHIYDENTIRKHTEQMNYLHPS 65
RK K +K+K+ S NIHI+ I+ Q NY+H S
Sbjct: 620 RKELKHATQKKIKIGSDCNT----NIHIFQYCAIKAEYRQSNYVHLS 492
>TC226623 similar to UP|Q9XH47 (Q9XH47) Small zinc finger-like protein,
partial (93%)
Length = 598
Score = 25.0 bits (53), Expect = 7.6
Identities = 9/16 (56%), Positives = 12/16 (74%)
Frame = -2
Query: 51 TIRKHTEQMNYLHPSL 66
+I KH++Q NY HP L
Sbjct: 336 SILKHSDQCNYFHPYL 289
>CD416038
Length = 370
Score = 25.0 bits (53), Expect = 7.6
Identities = 10/26 (38%), Positives = 19/26 (72%)
Frame = +3
Query: 4 IMELEFTSKPLQEKPRKCEKQEKLEK 29
+ME+EF+SK + PR+ E++ + E+
Sbjct: 258 LMEIEFSSKLICTVPRERERERERER 335
>TC204491 homologue to UP|GCST_PEA (P49364) Aminomethyltransferase,
mitochondrial precursor (Glycine cleavage system T
protein) (GCVT) , partial (16%)
Length = 393
Score = 25.0 bits (53), Expect = 7.6
Identities = 13/48 (27%), Positives = 24/48 (49%)
Frame = +2
Query: 21 CEKQEKLEKLKVKSTTEKGNMGNIHIYDENTIRKHTEQMNYLHPSLYL 68
C +Q +L + K ++ H+Y +NTI H + + YL+ +L
Sbjct: 92 CTRQAPK*RLLFEENPMKESLQKCHLYQQNTI-SHPDLLLYLYLKHFL 232
>TC225583 weakly similar to UP|O94285 (O94285) Rpc25 protein (Spbc2g5.07c
protein) , partial (11%)
Length = 1113
Score = 24.6 bits (52), Expect = 9.9
Identities = 10/38 (26%), Positives = 22/38 (57%)
Frame = +1
Query: 10 TSKPLQEKPRKCEKQEKLEKLKVKSTTEKGNMGNIHIY 47
+S+P +E PR+ + +L + +V++ + N H+Y
Sbjct: 28 SSQP*REAPRRSQGLRRLRRARVQT*RREQNHNQKHLY 141
>TC208001 similar to UP|Q84LW7 (Q84LW7) Peptidylprolyl cis-trans isomerase ,
partial (97%)
Length = 857
Score = 24.6 bits (52), Expect = 9.9
Identities = 17/57 (29%), Positives = 29/57 (50%), Gaps = 4/57 (7%)
Frame = +2
Query: 12 KPLQEKPRKCE---KQEKL-EKLKVKSTTEKGNMGNIHIYDENTIRKHTEQMNYLHP 64
K Q P C+ K +K+ E++KVK + + N+ +YD+N I + Q + P
Sbjct: 632 KQKQRLPHVCKPS*KPKKVKERVKVKQSRKLSNIYMSLVYDDNQIVMYYTQFSISLP 802
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.311 0.130 0.362
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,720,518
Number of Sequences: 63676
Number of extensions: 24879
Number of successful extensions: 167
Number of sequences better than 10.0: 30
Number of HSP's better than 10.0 without gapping: 166
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 167
length of query: 70
length of database: 12,639,632
effective HSP length: 46
effective length of query: 24
effective length of database: 9,710,536
effective search space: 233052864
effective search space used: 233052864
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 52 (24.6 bits)
Lotus: description of TM0269a.4